Q8MJ44

PR
Gene name
PRKACA
Protein name
cAMP-dependent protein kinase catalytic subunit alpha
Names
PKA C-alpha
Species
Canis lupus familiaris (Dog) (Canis familiaris)
KEGG Pathway
cfa:403556
EC number
2.7.11.11: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-63 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

183-203 (Activation loop from InterPro)

Target domain

43-297 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q8MJ44

Entry ID Method Resolution Chain Position Source
AF-Q8MJ44-F1 Predicted AlphaFoldDB

No variants for Q8MJ44

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8MJ44

No associated diseases with Q8MJ44

4 regional properties for Q8MJ44

Type Name Position InterPro Accession
domain Protein kinase domain 43 - 297 IPR000719
domain AGC-kinase, C-terminal 298 - 350 IPR000961
active_site Serine/threonine-protein kinase, active site 162 - 174 IPR008271
binding_site Protein kinase, ATP binding site 49 - 72 IPR017441

Functions

Description
EC Number 2.7.11.11 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
  • Membrane ; Lipid-anchor
  • Nucleus
  • Mitochondrion
  • Translocates into the nucleus (monomeric catalytic subunit)
  • The inactive holoenzyme is found in the cytoplasm
  • Distributed throughout the cytoplasm in meiotically incompetent oocytes
  • Associated to mitochondrion as meiotic competence is acquired
  • Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity)
  • Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (By similarity)
  • Recruited to the cell membrane through interaction with SMO (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
acrosomal vesicle A structure in the head of a spermatozoon that contains acid hydrolases, and is concerned with the breakdown of the outer membrane of the ovum during fertilization. It lies just beneath the plasma membrane and is derived from the lysosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
sperm flagellum A microtubule-based flagellum (or cilium) that is part of a sperm, a mature male germ cell that develops from a spermatid.

6 GO annotations of molecular function

Name Definition
AMP-activated protein kinase activity Catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein. This reaction requires the presence of AMP.
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
cAMP-dependent protein kinase activity cAMP-dependent catalysis of the reaction: ATP + a protein = ADP + a phosphoprotein.
protein kinase A regulatory subunit binding Binding to one or both of the regulatory subunits of protein kinase A.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.

3 GO annotations of biological process

Name Definition
cellular response to heat Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a heat stimulus, a temperature stimulus above the optimal temperature for that organism.
protein kinase A signaling A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase A, which occurs as a result of a single trigger reaction or compound.
protein phosphorylation The process of introducing a phosphate group on to a protein.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P00517 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Bos taurus (Bovine) PR
P16911 Pka-C2 cAMP-dependent protein kinase catalytic subunit 2 Drosophila melanogaster (Fruit fly) PR
P17612 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Homo sapiens (Human) PR
P05132 Prkaca cAMP-dependent protein kinase catalytic subunit alpha Mus musculus (Mouse) PR
P36887 PRKACA cAMP-dependent protein kinase catalytic subunit alpha Sus scrofa (Pig) PR
P21137 kin-1 cAMP-dependent protein kinase catalytic subunit Caenorhabditis elegans PR
10 20 30 40 50 60
MGNAAAKKGS EQESVKEFLA KAKEDFLKKW ENPAQNTAHL DQFERIKTLG TGSFGRVMLV
70 80 90 100 110 120
KHKETGNHFA MKILDKQKVV KLKQIEHTLN EKRILQAVNF PFLVKLEFSF KDNSNLYMVM
130 140 150 160 170 180
EYVPGGEMFS HLRRIGRFSE PHARFYAAQI VLTFEYLHSL DLIYRDLKPE NLLIDQQGYI
190 200 210 220 230 240
QVTDFGFAKR VKGRTWTLCG TPEYLAPEII LSKGYNKAVD WWALGVLIYE MAAGYPPFFA
250 260 270 280 290 300
DQPIQIYEKI VSGKVRFPSH FSSDLKDLLR NLLQVDLTKR FGNLKNGVND IKNHKWFATT
310 320 330 340
DWIAIYQRKV EAPFIPKFKG PGDTSNFDDY EEEEIRVSIN EKCGKEFCEF