Q8K1R7

SS
Gene name
Nek9 (Nercc)
Protein name
Serine/threonine-protein kinase Nek9
Names
Nercc1 kinase, Never in mitosis A-related kinase 9, NimA-related protein kinase 9
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:217718
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

Nek9 is a NIMA-related kinase essential for proper mitotic spindle assembly. A human homolog Nek7 (Q8TDX7) is subject to autoinhibition by a tyrosine motif, Tyr97, like human Nek6 (Tyr108). This is because the Y97A mutation is more active than wild-type Nek7. A similar tyrosine motif is also found in mouse Nek9.

Autoinhibitory domains (AIDs)

114-119 (Tyrosine motif) SS

Target domain

20-30 (N-terminal extension of kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

193-216 (Activation loop from InterPro)

Target domain

52-308 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

2 structures for Q8K1R7

Entry ID Method Resolution Chain Position Source
5DE2 X-ray 278 A C/D 817-835 PDB
AF-Q8K1R7-F1 Predicted AlphaFoldDB

8 variants for Q8K1R7

Variant ID(s) Position Change Description Diseaes Association Provenance
rs48069342 337 A>T No Ensembl
rs224928465 498 N>T No Ensembl
rs29171478 635 K>R No Ensembl
rs219134055 870 R>H No Ensembl
rs246500530 880 D>N No Ensembl
rs49811706 883 V>M No Ensembl
rs244302148 893 A>P No Ensembl
rs229439348 909 A>G No Ensembl

No associated diseases with Q8K1R7

2 regional properties for Q8K1R7

Type Name Position InterPro Accession
domain Cytidyltransferase-like domain 22 - 153 IPR004821
domain CTP:phosphocholine cytidylyltransferase domain 19 - 170 IPR041723

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
metal ion binding Binding to a metal ion.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

2 GO annotations of biological process

Name Definition
cell cycle The progression of biochemical and morphological phases and events that occur in a cell during successive cell replication or nuclear replication events. Canonically, the cell cycle comprises the replication and segregation of genetic material followed by the division of the cell, but in endocycles or syncytial cells nuclear replication or nuclear division may not be followed by cell division.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.

9 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P51957 NEK4 Serine/threonine-protein kinase Nek4 Homo sapiens (Human) PR
Q6P3R8 NEK5 Serine/threonine-protein kinase Nek5 Homo sapiens (Human) PR
Q8NE28 STKLD1 Serine/threonine kinase-like domain-containing protein STKLD1 Homo sapiens (Human) PR
Q7TSC3 Nek5 Serine/threonine-protein kinase Nek5 Mus musculus (Mouse) PR
Q10GB1 NEK1 Serine/threonine-protein kinase Nek1 Oryza sativa subsp japonica (Rice) PR
Q6ZEZ5 NEK3 Serine/threonine-protein kinase Nek3 Oryza sativa subsp japonica (Rice) PR
Q9SLI2 NEK1 Serine/threonine-protein kinase Nek1 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LT35 NEK6 Serine/threonine-protein kinase Nek6 Arabidopsis thaliana (Mouse-ear cress) PR
Q8RXT4 NEK4 Serine/threonine-protein kinase Nek4 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MSVLGEYERH CDSINSDFGS ESGGGGDSGP GPSAVPGPRA GGGAAEQEEL HYIPIRVLGR
70 80 90 100 110 120
GAFGEATLYR RTEDDSLVVW KEVDLTRLSE KERRDALNEI VILALLQHDN IIAYYNHFMD
130 140 150 160 170 180
NTTLLIELEY CNGGNLYDKI LRQKDKLFEE EMVVWYLFQI VSAVSCIHKA GILHRDIKTL
190 200 210 220 230 240
NIFLTKANLI KLGDYGLAKK LNSEYSMAET LVGTPYYMSP ELCQGVKYNF KSDIWAVGCV
250 260 270 280 290 300
IFELLTLKRT FDATNPLNLC VKIVQGIRAM EVDSSQYSLE LIQLVHACLD QDPEQRPAAD
310 320 330 340 350 360
ALLDLPLLRT RRREMEEKVT LLNAPTKRPR SSTVTEAPIA VVTSRTSEVY VWGGGKSTPQ
370 380 390 400 410 420
KLDVIKSGCS ARQVCAGNTH FAVVTVEKEL YTWVNMQGGT KLHGQLGHGD KASYRQPKHV
430 440 450 460 470 480
EKLQGKAIHQ VSCGDDFTVC VTDEGQLYAF GSDYYGCMGV DKVSGPEVLE PMQLNFFLSN
490 500 510 520 530 540
PVEQVSCGDN HVVVLTRNKE VYSWGCGEYG RLGLDSEEDY YTPQRVDVPK ALIIVAVQCG
550 560 570 580 590 600
CDGTFLLTQS GKVLACGLNE FNKLGLNQCM SGIINHEAYH EVPYTTSFTL AKQLSFYKIR
610 620 630 640 650 660
TIAPGKTHTA AIDERGRLLT FGCNKCGQLG VGNYKKRLGI NLLGGPLGGK QVIRVSCGDE
670 680 690 700 710 720
FTIAATDDNH IFAWGNGGNG RLAMTPTERP HGSDICTSWP RPIFGSLHHV PDLSCRGWHT
730 740 750 760 770 780
ILIVEKVLNS KTIRSNSSGL SIGTVVQSSS PGGGIGGGGG GGGGGGGEEE DSQQESETPD
790 800 810 820 830 840
PSGGFRGTME ADRGMEGLIS PTEAVGNSCG ASSSCPGWLR KELENAEFIP MPDSPAPLSA
850 860 870 880 890 900
AFSQSEKDTL PYEELQGLKV ASEVPPEPQR AAGAWPPRLD PAVPCVGKAL TSAACACSAL
910 920 930 940 950 960
QVEVDRLQAL VLKCLEEQQK LQQENLQMFT QLQKLNKKLE GGQQVGMHSR GTQTAKEEME
970 980
MDPKPDLDSE SWCLLGTDSC RPSL