Q8INB9

SS
Gene name
Akt (Akt1, CG4006)
Protein name
RAC serine/threonine-protein kinase
Names
DAkt , DRAC-PK , Dakt1 , EC 2.7.11.1 , Protein kinase B , PKB
Species
Drosophila melanogaster (Fruit fly)
KEGG Pathway
dme:Dmel_CG4006
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

Akt is a serine/threonine kinase that belongs to a group of proteins called the AGC superfamily. It is involved in various biological responses through the phosphatidylinositol 3-kinase (PI3K) signal transduction pathway. Akt2, in particular, is regulated by different regions of the protein.<br>The N-terminal PH domain of Akt2 plays a regulatory role in its activation. Phosphorylation of Ser474 in the C-terminal regulatory domain is also necessary for full activation of Akt2. These modifications help activate the kinase and enable it to carry out its functions.<br>The linker region between the N-lobe and C-lobe of Akt2 occupies the ATP binding site. Additionally, the activation loop within Akt2 aids in autoinhibition by blocking the binding of peptide substrates, while still allowing access to the ATP binding site.

Autoinhibitory domains (AIDs)

260-554 (Linker region between N- and C-lobes) SS

Target domain

253-611 (Kinase domain)

Relief mechanism

PTM

Assay

108-209 (PH domain) SS

Target domain

266-523 (Protein kinase domain)

Relief mechanism

Ligand binding, PTM

Assay

Accessory elements

406-429 (Activation loop from InterPro)

Target domain

266-524 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q8INB9

Entry ID Method Resolution Chain Position Source
AF-Q8INB9-F1 Predicted AlphaFoldDB

No variants for Q8INB9

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8INB9

No associated diseases with Q8INB9

3 regional properties for Q8INB9

Type Name Position InterPro Accession
domain Pyruvate kinase, barrel 21 - 347 IPR015793
domain Pyruvate kinase, C-terminal 382 - 499 IPR015795
active_site Pyruvate kinase, active site 237 - 249 IPR018209

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm, cytosol
  • Cell membrane
  • Recruited to plasma membrane upon activation
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

36 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
cholesterol homeostasis Any process involved in the maintenance of an internal steady state of cholesterol within an organism or cell.
circadian rhythm Any biological process in an organism that recurs with a regularity of approximately 24 hours.
dendrite regeneration The regrowth of dendrites in response to their loss or damage.
epithelial cell migration, open tracheal system The orderly movement of epithelial cells during development of an open tracheal system. An example of this is found in Drosophila melanogaster.
insulin receptor signaling pathway The series of molecular signals generated as a consequence of the insulin receptor binding to insulin.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
lipid homeostasis Any process involved in the maintenance of an internal steady state of lipid within an organism or cell.
long-term synaptic depression A process that modulates synaptic plasticity such that synapses are changed resulting in the decrease in the rate, or frequency of synaptic transmission at the synapse.
multicellular organism growth The increase in size or mass of an entire multicellular organism, as opposed to cell growth.
negative regulation of apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process.
negative regulation of hippo signaling Any process that stops, prevents, or reduces the frequency, rate or extent of hippo signaling.
negative regulation of neuron apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process in neurons.
negative regulation of peptide hormone secretion Any process that decreases the rate, frequency, or extent of the regulated release of a peptide hormone from secretory granules.
negative regulation of synaptic assembly at neuromuscular junction Any process that stops, prevents, or reduces the frequency, rate or extent of synaptic assembly at neuromuscular junction.
negative regulation of triglyceride catabolic process Any process that decreases the frequency, rate, or extent of the chemical reactions and pathways resulting in the breakdown of triglyceride.
oogenesis The complete process of formation and maturation of an ovum or female gamete from a primordial female germ cell. Examples of this process are found in Mus musculus and Drosophila melanogaster.
open tracheal system development The process whose specific outcome is the progression of an open tracheal system over time, from its formation to the mature structure. An open tracheal system is a respiratory system, a branched network of epithelial tubes that supplies oxygen to target tissues via spiracles. An example of this is found in Drosophila melanogaster.
phosphatidylinositol 3-kinase/protein kinase B signal transduction A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase B (also called AKT), which occurs as a result of a single trigger reaction or compound.
positive regulation of axon regeneration Any process that activates, maintains or increases the rate of axon regeneration.
positive regulation of border follicle cell migration Any process that activates or increases the frequency, rate or extent of border follicle cell migration.
positive regulation of cell growth Any process that activates or increases the frequency, rate, extent or direction of cell growth.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
positive regulation of cell size Any process that increases cell size.
positive regulation of lipid storage Any process that increases the rate, frequency or extent of lipid storage. Lipid storage is the accumulation and maintenance in cells or tissues of lipids, compounds soluble in organic solvents but insoluble or sparingly soluble in aqueous solvents. Lipid reserves can be accumulated during early developmental stages for mobilization and utilization at later stages of development.
positive regulation of multicellular organism growth Any process that activates or increases the frequency, rate or extent of growth of an organism to reach its usual body size.
positive regulation of organ growth Any process that activates or increases the frequency, rate or extent of growth of an organ of an organism.
positive regulation of TORC1 signaling Any process that activates or increases the frequency, rate or extent of TORC1 signaling.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of dendrite development Any process that modulates the frequency, rate or extent of dendrite development.
regulation of hemocyte proliferation Any process that modulates the frequency, rate or extent of hemocyte proliferation. Hemocytes are blood cells associated with a hemocoel (the cavity containing most of the major organs of the arthropod body) which are involved in defense and clotting of hemolymph, but not involved in transport of oxygen. An example of this is found in Drosophila melanogaster.
regulation of multicellular organism growth Any process that modulates the frequency, rate or extent of growth of the body of an organism so that it reaches its usual body size.
response to oxidative stress Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of oxidative stress, a state often resulting from exposure to high levels of reactive oxygen species, e.g. superoxide anions, hydrogen peroxide (H2O2), and hydroxyl radicals.
vascular endothelial growth factor receptor signaling pathway The series of molecular signals initiated by a ligand binding to a vascular endothelial growth factor receptor (VEGFR) on the surface of the target cell, and ending with the regulation of a downstream cellular process, e.g. transcription.
wound healing, spreading of cells The migration of a cell along or through a wound gap that contributes to the reestablishment of a continuous surface.

22 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q01314 AKT1 RAC-alpha serine/threonine-protein kinase Bos taurus (Bovine) SS
Q9W0V1 Pdk1 3-phosphoinositide-dependent protein kinase 1 Drosophila melanogaster (Fruit fly) SS
A1Z7T0 Pkn Serine/threonine-protein kinase N Drosophila melanogaster (Fruit fly) SS
P83099 Pkcdelta Putative protein kinase C delta type homolog Drosophila melanogaster (Fruit fly) PR
P13677 inaC Protein kinase C, eye isozyme Drosophila melanogaster (Fruit fly) SS
P05130 Pkc53E Protein kinase C, brain isozyme Drosophila melanogaster (Fruit fly) SS
Q9NBK5 trc Serine/threonine-protein kinase tricornered Drosophila melanogaster (Fruit fly) SS
A1Z9X0 aPKC Atypical protein kinase C Drosophila melanogaster (Fruit fly) SS
P31749 AKT1 RAC-alpha serine/threonine-protein kinase Homo sapiens (Human) EV
P31751 AKT2 RAC-beta serine/threonine-protein kinase Homo sapiens (Human) EV SS
Q9Y243 AKT3 RAC-gamma serine/threonine-protein kinase Homo sapiens (Human) SS
Q9WUA6 Akt3 RAC-gamma serine/threonine-protein kinase Mus musculus (Mouse) PR
P31750 Akt1 RAC-alpha serine/threonine-protein kinase Mus musculus (Mouse) PR
Q60823 Akt2 RAC-beta serine/threonine-protein kinase Mus musculus (Mouse) PR
P47197 Akt2 RAC-beta serine/threonine-protein kinase Rattus norvegicus (Rat) SS
Q63484 Akt3 RAC-gamma serine/threonine-protein kinase Rattus norvegicus (Rat) PR
P47196 Akt1 RAC-alpha serine/threonine-protein kinase Rattus norvegicus (Rat) PR
Q17941 akt-1 Serine/threonine-protein kinase akt-1 Caenorhabditis elegans PR
Q9XTG7 akt-2 Serine/threonine-protein kinase akt-2 Caenorhabditis elegans SS
Q9SUA3 D6PKL1 Serine/threonine-protein kinase D6PKL1 Arabidopsis thaliana (Mouse-ear cress) PR
O64682 PID Protein kinase PINOID Arabidopsis thaliana (Mouse-ear cress) PR
Q9LUL2 WAG2 Serine/threonine-protein kinase WAG2 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MNYLPFVLQR RSTVVASAPA PGSASRIPES PTTTGSNIIN IIYSQSTHPN SSPTSGSAEK
70 80 90 100 110 120
FSWQQSWPSR TSAAPTHDSG TMSINTTFDL SSPSVTSGHA LTEQTQVVKE GWLMKRGEHI
130 140 150 160 170 180
KNWRQRYFVL HSDGRLMGYR SKPADSASTP SDFLLNNFTV RGCQIMTVDR PKPFTFIIRG
190 200 210 220 230 240
LQWTTVIERT FAVESELERQ QWTEAIRNVS SRLIDVGEVA MTPSEQTDMT DVDMATIAED
250 260 270 280 290 300
ELSEQFSVQG TTCNSSGVKK VTLENFEFLK VLGKGTFGKV ILCREKATAK LYAIKILKKE
310 320 330 340 350 360
VIIQKDEVAH TLTESRVLKS TNHPFLISLK YSFQTNDRLC FVMQYVNGGE LFWHLSHERI
370 380 390 400 410 420
FTEDRTRFYG AEIISALGYL HSQGIIYRDL KLENLLLDKD GHIKVADFGL CKEDITYGRT
430 440 450 460 470 480
TKTFCGTPEY LAPEVLDDND YGQAVDWWGT GVVMYEMICG RLPFYNRDHD VLFTLILVEE
490 500 510 520 530 540
VKFPRNITDE AKNLLAGLLA KDPKKRLGGG KDDVKEIQAH PFFASINWTD LVLKKIPPPF
550 560 570 580 590 600
KPQVTSDTDT RYFDKEFTGE SVELTPPDPT GPLGSIAEEP LFPQFSYQGD MASTLGTSSH
610
ISTSTSLASM Q