Q8H0U8

SS
Gene name
RH42 (RCF1, At1g20920, F9H16.10)
Protein name
DEAD-box ATP-dependent RNA helicase 42
Names
EC 3.6.4.13 , DEAD-box RNA helicase RCF1 , REGULATOR OF CBF GENE EXPRESSION 1
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT1G20920
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The DEAD-box adenosine triphosphatase (ATPase) Prp5p facilitates U2 small nuclear ribonucleoprotein particle (snRNP) binding to the intron branch site region during spliceosome assembly. Motifs in the DEAD box helicase domain are involved in ATP binding and ATP hydrolysis, whereas motifs in C-terminal helicase domain, potentially responsible for RNA-binding or ATP coordination.<br>There is a unique interdomain interaction between the DEAD box helicase domain and helicase domain in which the C-terminal helicase domain clashes with the RNA-binding surface. Destabilizing this interaction accelerates RNA duplex unwinding, suggesting that it is present in solution and inhibitory for catalysis. The N-terminal flanking sequence to the DEAD box helicase interacts with DEAD box helicase domain and C-terminal helicase domain, specifically the linker region between the two domains, and stabilizes the autoinhibited state.

Autoinhibitory domains (AIDs)

530-741 (DEAD box helicase domain) SS

Target domain

751-880 (C-terminal helicase domain)

Relief mechanism

Assay

751-880 (C-terminal helicase domain) SS

Target domain

530-741 (DEAD box helicase domain)

Relief mechanism

Assay

481-529 (N-terminal flanking sequence) SS

Target domain

530-741 (DEAD box helicase domain);751-880 (C-terminal helicase domain)

Relief mechanism

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q8H0U8

Entry ID Method Resolution Chain Position Source
AF-Q8H0U8-F1 Predicted AlphaFoldDB

46 variants for Q8H0U8

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH01078704 8 Y>F No 1000Genomes
ENSVATH00034836 8 Y>H No 1000Genomes
ENSVATH01078705 32 S>I No 1000Genomes
tmp_1_7285455_T_G 38 D>E No 1000Genomes
tmp_1_7285463_C_T 41 S>L No 1000Genomes
tmp_1_7285577_G_C 79 R>T No 1000Genomes
ENSVATH13935252 101 S>F No 1000Genomes
tmp_1_7285666_G_A 109 D>N No 1000Genomes
ENSVATH13935253 111 R>K No 1000Genomes
ENSVATH13935254 136 R>K No 1000Genomes
ENSVATH11666803 154 R>S No 1000Genomes
ENSVATH11666804 177 R>Q No 1000Genomes
ENSVATH04612563 208 E>A No 1000Genomes
ENSVATH11666925 252 V>L No 1000Genomes
tmp_1_7286120_A_T 260 E>V No 1000Genomes
tmp_1_7286204_A_G 288 E>G No 1000Genomes
ENSVATH13935265 301 S>N No 1000Genomes
ENSVATH04612564 305 G>V No 1000Genomes
ENSVATH11666928 307 A>G No 1000Genomes
ENSVATH11666927 307 A>S No 1000Genomes
tmp_1_7286389_G_T 350 D>Y No 1000Genomes
tmp_1_7286461_G_A 374 A>T No 1000Genomes
ENSVATH04612565 390 M>I No 1000Genomes
ENSVATH11666929 393 P>H No 1000Genomes
ENSVATH11666929 393 P>L No 1000Genomes
ENSVATH11666930 396 E>D No 1000Genomes
ENSVATH13935266 403 P>S No 1000Genomes
ENSVATH11666931 416 M>T No 1000Genomes
ENSVATH11666932 418 G>W No 1000Genomes
ENSVATH00034837 421 S>N No 1000Genomes
ENSVATH04612566 449 P>S No 1000Genomes
tmp_1_7286792_T_C 484 I>T No 1000Genomes
ENSVATH04612567 488 P>S No 1000Genomes
tmp_1_7287112_A_G 591 I>V No 1000Genomes
ENSVATH00034838 678 R>Q No 1000Genomes
ENSVATH13935268 762 R>W No 1000Genomes
ENSVATH04612572 984 A>T No 1000Genomes
tmp_1_7288294_A_G 985 T>A No 1000Genomes
ENSVATH13935269 991 A>T No 1000Genomes
tmp_1_7288403_C_T 1021 A>V No 1000Genomes
tmp_1_7288409_T_C 1023 M>T No 1000Genomes
ENSVATH00034839 1031 V>G No 1000Genomes
ENSVATH04612574 1035 P>S No 1000Genomes
tmp_1_7288682_C_T 1114 P>L No 1000Genomes
ENSVATH11667001 1116 P>S No 1000Genomes
ENSVATH11667002 1135 H>Q No 1000Genomes

No associated diseases with Q8H0U8

5 regional properties for Q8H0U8

Type Name Position InterPro Accession
conserved_site ATP-dependent RNA helicase DEAD-box, conserved site 684 - 692 IPR000629
domain Helicase, C-terminal domain-like 749 - 910 IPR001650
domain DEAD/DEAH box helicase domain 554 - 726 IPR011545
domain Helicase superfamily 1/2, ATP-binding domain 548 - 753 IPR014001
domain RNA helicase, DEAD-box type, Q motif 529 - 557 IPR014014

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

1 GO annotations of biological process

Name Definition
mRNA splicing, via spliceosome The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.

19 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P21372 PRP5 Pre-mRNA-processing ATP-dependent RNA helicase PRP5 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) EV
Q9VNP5 BoYb Putative ATP-dependent RNA helicase BoYb Drosophila melanogaster (Fruit fly) PR
Q9VL52 SoYb Putative ATP-dependent RNA helicase SoYb Drosophila melanogaster (Fruit fly) PR
Q7L014 DDX46 Probable ATP-dependent RNA helicase DDX46 Homo sapiens (Human) PR
Q569Z5 Ddx46 Probable ATP-dependent RNA helicase DDX46 Mus musculus (Mouse) PR
Q62780 Ddx46 Probable ATP-dependent RNA helicase DDX46 Rattus norvegicus (Rat) PR
Q9SA27 RH36 DEAD-box ATP-dependent RNA helicase 36 Arabidopsis thaliana (Mouse-ear cress) PR
P41377 TIF4A-2 Eukaryotic initiation factor 4A-2 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FVV4 RH55 DEAD-box ATP-dependent RNA helicase 55 Arabidopsis thaliana (Mouse-ear cress) PR
Q9CAI7 TIF4A-3 Eukaryotic initiation factor 4A-3 Arabidopsis thaliana (Mouse-ear cress) PR
O80792 RH33 Putative DEAD-box ATP-dependent RNA helicase 33 Arabidopsis thaliana (Mouse-ear cress) PR
O22907 RH24 DEAD-box ATP-dependent RNA helicase 24 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LIH9 RH51 DEAD-box ATP-dependent RNA helicase 51 Arabidopsis thaliana (Mouse-ear cress) PR
Q56X76 RH39 DEAD-box ATP-dependent RNA helicase 39 Arabidopsis thaliana (Mouse-ear cress) PR
Q8GY84 RH10 DEAD-box ATP-dependent RNA helicase 10 Arabidopsis thaliana (Mouse-ear cress) PR
Q9FFQ1 RH31 DEAD-box ATP-dependent RNA helicase 31 Arabidopsis thaliana (Mouse-ear cress) PR
Q9SB89 RH27 DEAD-box ATP-dependent RNA helicase 27 Arabidopsis thaliana (Mouse-ear cress) PR
P41376 EIF4A1 Eukaryotic initiation factor 4A-1 Arabidopsis thaliana (Mouse-ear cress) PR
Q84TG1 RH57 DEAD-box ATP-dependent RNA helicase 57 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MEVEKSKYRS EDLDVVEEEA DLKKSRRDRD RSNERKKDKG SEKRREKDRR KKRVKSSDSE
70 80 90 100 110 120
DDYDRDDDEE REKRKEKERE RRRRDKDRVK RRSERRKSSD SEDDVEEEDE RDKRRVNEKE
130 140 150 160 170 180
RGHREHERDR GKDRKRDRER EERKDKERER EKDRERRERE REEREKERVK ERERREREDG
190 200 210 220 230 240
ERDRREREKE RGSRRNRERE RSREVGNEES DDDVKRDLKR RRKEGGERKE KEREKSVGRS
250 260 270 280 290 300
SRHEDSPKRK SVEDNGEKKE KKTREEELED EQKKLDEEVE KRRRRVQEWQ ELKRKKEEAE
310 320 330 340 350 360
SESKGDADGN EPKAGKAWTL EGESDDEEGH PEEKSETEMD VDEETKPEND GDAKMVDLEN
370 380 390 400 410 420
ETAATVSESG GDGAVDEEEI DPLDAFMNTM VLPEVEKFCN GAPPPAVNDG TLDSKMNGKE
430 440 450 460 470 480
SGDRPKKGFN KALGRIIQGE DSDSDYSEPK NDDDPSLDED DEEFMKRVKK TKAEKLSLVD
490 500 510 520 530 540
HSKIEYEPFR KNFYIEVKDI SRMTQEEVNT YRKELELKVH GKDVPRPIKF WHQTGLTSKI
550 560 570 580 590 600
LDTMKKLNYE KPMPIQTQAL PIIMSGRDCI GVAKTGSGKT LGFVLPMLRH IKDQPPVEAG
610 620 630 640 650 660
DGPIGLVMAP TRELVQQIHS DIRKFSKPLG IRCVPVYGGS GVAQQISELK RGTEIVVCTP
670 680 690 700 710 720
GRMIDILCTS SGKITNLRRV TFLVMDEADR MFDMGFEPQI TRIIQNIRPE RQTVLFSATF
730 740 750 760 770 780
PRQVETLARK VLNKPVEIQV GGRSVVNKDI TQLVEVRPES DRFLRLLELL GEWSEKGKIL
790 800 810 820 830 840
VFVQSQEKCD ALYRDMIKSS YPCLSLHGGK DQTDRESTIS DFKNDVCNLL IATSVAARGL
850 860 870 880 890 900
DVKELELVVN FDAPNHYEDY VHRVGRTGRA GRKGCAVTFI SEDDAKYAPD LVKALELSEQ
910 920 930 940 950 960
PVPDDLKALA DGFMVKVKQG IEQAHGTGYG GSGFKFNEEE EEVRKAAKKA QAKEYGFEED
970 980 990 1000 1010 1020
KSDSEDENDV VRKAGGGEIS QQQATFAQIA AIAAAAKAAA AAPVSAPVTA NQLLANGGGL
1030 1040 1050 1060 1070 1080
AAMPGVLPVT VPTLPSEGAG RAAAMVAAMN LQHNLAKIQA DAMPEHYEAE LEINDFPQNA
1090 1100 1110 1120 1130 1140
RWKVTHKETL GPISEWTGAA ITTRGQFYPT GRIPGPGERK LYLFIEGPSE KSVKHAKAEL
1150 1160
KRVLEDITNQ AMSSLPGGAS GRYSVL