Q8BRT1

SS
Gene name
Clasp2 (Kiaa0627)
Protein name
CLIP-associating protein 2
Names
Cytoplasmic linker-associated protein 2
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:76499
EC number
Protein Class

Descriptions

CLASPs, major microtubule-stabilizing factors in interphase and mitosis, prevent microtubule from switching form growth to shortening by stabilizing growing microtubule ends, and thus suppress microtubule catastrophes. CLASP2a consists of three TOG-like domains (termed TOG1, 2, and 3) and a C-terminal domain, CLIP-interacting domain (CLIP-ID) responsible for interaction with CLIP-170 and other partners. CLASP2a TOG2 is necessary and sufficient for catastrophe inhibition. The inhibition function is suppressed by the C-terminal domain CLIP-ID while the TOG1 domain or the CLIP-ID partner binding to CLIP-ID can release the autoinhibition.

Autoinhibitory domains (AIDs)

1169-1278 (C-terminal domain CLIP-ID) SS

Target domain

640-877 (TOG2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q8BRT1

Entry ID Method Resolution Chain Position Source
3WOZ X-ray 220 A A/B/C/D 642-873 PDB
AF-Q8BRT1-F1 Predicted AlphaFoldDB

No variants for Q8BRT1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q8BRT1

No associated diseases with Q8BRT1

6 regional properties for Q8BRT1

Type Name Position InterPro Accession
domain Arf GTPase activating protein 1 - 124 IPR001164
repeat Ankyrin repeat 132 - 228 IPR002110
domain GIT, Spa2 homology (SHD) domain 273 - 303 IPR013724-1
domain GIT, Spa2 homology (SHD) domain 337 - 367 IPR013724-2
domain ARF GTPase-activating protein GIT1, C-terminal 652 - 764 IPR022018
domain Arf GTPase-activating protein GIT1/2, coiled-coil domain 418 - 482 IPR032352

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Chromosome, centromere, kinetochore
  • Cytoplasm, cytoskeleton, spindle
  • Cytoplasm, cytoskeleton, spindle pole
  • Golgi apparatus
  • Golgi apparatus, trans-Golgi network
  • Cell membrane
  • Cell projection, ruffle membrane
  • Localizes to microtubule plus ends (PubMed:11290329)
  • Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase
  • Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase
  • In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella
  • Localizes to the cell cortex and this requires ERC1 and PHLDB2 (By similarity)
  • Also localizes to meiotic spindle poles
  • The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

16 GO annotations of cellular component

Name Definition
basal cortex The region that lies just beneath the plasma membrane on the basal edge of a cell.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
cell leading edge The area of a motile cell closest to the direction of movement.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasmic microtubule Any microtubule in the cytoplasm of a cell.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
mitotic spindle A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
ruffle membrane The portion of the plasma membrane surrounding a ruffle.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.
spindle pole Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.
trans-Golgi network The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.

2 GO annotations of molecular function

Name Definition
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule plus-end binding Binding to the plus end of a microtubule.

19 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
establishment of cell polarity The specification and formation of anisotropic intracellular organization or cell growth patterns.
establishment of mitotic spindle localization The cell cycle process in which the directed movement of the mitotic spindle to a specific location in the cell occurs.
establishment or maintenance of cell polarity Any cellular process that results in the specification, formation or maintenance of anisotropic intracellular organization or cell growth patterns.
exit from mitosis The cell cycle transition where a cell leaves M phase and enters a new G1 phase. M phase is the part of the mitotic cell cycle during which mitosis and cytokinesis take place.
fibroblast migration Cell migration that is accomplished by extension and retraction of a fibroblast pseudopodium. A fibroblast is a connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules.
Golgi organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the Golgi apparatus.
meiotic cell cycle Progression through the phases of the meiotic cell cycle, in which canonically a cell replicates to produce four offspring with half the chromosomal content of the progenitor cell via two nuclear divisions.
microtubule anchoring Any process in which a microtubule is maintained in a specific location in a cell.
microtubule cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
microtubule depolymerization The removal of tubulin heterodimers from one or both ends of a microtubule.
microtubule nucleation The process in which tubulin alpha-beta heterodimers begin aggregation to form an oligomeric tubulin structure (a microtubule seed). Microtubule nucleation is the initiating step in the formation of a microtubule in the absence of any existing microtubules ('de novo' microtubule formation).
microtubule organizing center organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a microtubule organizing center, a structure from which microtubules grow.
mitotic spindle assembly Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
negative regulation of microtubule depolymerization Any process that stops, prevents, or reduces the frequency, rate or extent of microtubule depolymerization; prevention of depolymerization of a microtubule can result from binding by 'capping' at the plus end (e.g. by interaction with another cellular protein of structure) or by exposing microtubules to a stabilizing drug such as taxol.
positive regulation of protein localization to membrane Any process that activates or increases the frequency, rate or extent of protein localization to membrane.
regulation of microtubule-based process Any process that modulates the frequency, rate or extent of any cellular process that depends upon or alters the microtubule cytoskeleton.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38198 STU1 Protein STU1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9NBD7 chb CLIP-associating protein Drosophila melanogaster (Fruit fly) SS
Q7Z460 CLASP1 CLIP-associating protein 1 Homo sapiens (Human) SS
O75122 CLASP2 CLIP-associating protein 2 Homo sapiens (Human) EV
Q80TV8 Clasp1 CLIP-associating protein 1 Mus musculus (Mouse) SS
Q99JD4 Clasp2 CLIP-associating protein 2 Rattus norvegicus (Rat) SS
Q6NYW6 clasp2 CLIP-associating protein 2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MRRLICKRIC DYKSFDDEES VDGNRPSSAA SAFKVPAPKT PGNPVSSARK PGSAGGPKVG
70 80 90 100 110 120
GPSKEGGAGA VDEDDFIKAF TDVPSVQIYS SRELEETLNK IREILSDDKH DWDQRANALK
130 140 150 160 170 180
KIRSLLVAGA AQYDCFFQHL RLLDGALKLS AKDLRSQVVR EACITVAHLS TVLGNKFDHG
190 200 210 220 230 240
AEAIVPTLFN LVPNSAKVMA TSGCAAIRFI IRHTHVPRLI PLITSNCTSK SVPVRRRSFE
250 260 270 280 290 300
FLDLLLQEWQ THSLERHAAV LVETIKKGIH DADAEARVEA RKTYMGLRNH FPGEAETLYN
310 320 330 340 350 360
SLEPSYQKSL QTYLKSSGSV ASLPQSDRSS SSSQESLNRP FSSKWSTANP STVAGRVSVG
370 380 390 400 410 420
GSKANPLPGS LQRSRSDIDV NAAAGAKAHH AAGQAVRSGR LGAGALNPGS YASLEDTSDK
430 440 450 460 470 480
MDGTASDDGR VRAKLSTPLV AVGNAKTDSR GRSRTKMVSQ SQPGSRSGSP GRVLTTTALS
490 500 510 520 530 540
TVSSGAQRVL VNSASAQKRS KIPRSQGCSR EASPSRLSVA RSSRIPRPSV SQGCSREASR
550 560 570 580 590 600
ESSRDTSPVR SFQPLGPGYG ISQSSRLSSS VSAMRVLNTG SDVEEAVADA LLLGDIRTKK
610 620 630 640 650 660
KPARRRYESY GMHSDDDANS DASSACSERS YSSRNGSIPT YMRQTEDVAE VLNRCASSNW
670 680 690 700 710 720
SERKEGLLGL QNLLKNQRTL SRIELKRLCE IFTRMFADPH GKVFSMFLET LVDFIQVHKD
730 740 750 760 770 780
DLQDWLFVLL TQLLKKMGAD LLGSVQAKVQ KALDITRESF PNDLQFNILM RFTVDQTQTP
790 800 810 820 830 840
SLKVKVAILK YIETLAKQMD PRDFTNSSET RLAVSRVITW TTEPKSSDVR KAAQSVLISL
850 860 870 880 890 900
FELNTPEFTM LLGALPKTFQ DGATKLLHNH LRNTGNGTQS SMGSPLTRPT PRSPANWSSP
910 920 930 940 950 960
LTSPTNTSQN TLSPSAFDYD TENMNSEDIY SSLRGVTEAI QNFSFRSQED MSEPVRRDPK
970 980 990 1000 1010 1020
KEDGDTICSG PGMSDPRAGG DAADGSQPAL DNKASLLHSM PLHSSPRSRD YNPYNYSDSI
1030 1040 1050 1060 1070 1080
SPFNKSALKE AMFDDDADQF PDDLSLDHSD LVAELLKELS NHNERIEERK IALYELMKLT
1090 1100 1110 1120 1130 1140
QEESFSVWDE HFKTILLLLL ETLGDKEPTI RALALKVLKE ILRHQPARFK NYAELTVMKT
1150 1160 1170 1180 1190 1200
LEAHKDPHKE VVRSAEEAAS VLATSISPEQ CIKVLCPIIQ TADYPINLAA IKMQTKVIER
1210 1220 1230 1240 1250 1260
VSKETLNMLL PEIMPGLIQG YDNSESSVRK ACVFCLVAVH AVIGDELKPH LSQLTGSKMK
1270 1280
LLNLYIKRAQ TGSAGADPTA DVSGQS