Q8AYC9

PR
Gene name
CHEK1 (CHK1)
Protein name
Serine/threonine-protein kinase Chk1
Names
CHK1 checkpoint homolog, Checkpoint kinase-1
Species
Gallus gallus (Chicken)
KEGG Pathway
gga:374260
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

(Annotation based on sequence homology with O14757)
CHEK1 is a serine/threonine protein kinase and is a key mediator that links the DNA stress responses to the cell cycle. Full-length CHEK1 has only low basal activity when expressed. Truncation of the C-terminal regulatory region activates CHEK1 even in the absence of stress.

Autoinhibitory domains (AIDs)

245-446 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

147-172 (Activation loop from InterPro)

Target domain

9-265 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q8AYC9

Entry ID Method Resolution Chain Position Source
AF-Q8AYC9-F1 Predicted AlphaFoldDB

38 variants for Q8AYC9

Variant ID(s) Position Change Description Diseaes Association Provenance
rs732764600 24 Q>R No Ensembl
rs736470190 47 D>G No Ensembl
rs734403832 48 C>G No Ensembl
rs732304398 66 N>T No Ensembl
rs737056156 76 E>G No Ensembl
rs735329243 81 Y>* No Ensembl
rs732373230 119 V>G No Ensembl
rs741214714 127 T>P No Ensembl
rs733665222 128 H>P No Ensembl
rs737184686 142 D>A No Ensembl
rs740224115 156 K>N No Ensembl
rs736045480 168 C>G No Ensembl
rs739446303 168 C>W No Ensembl
rs739049862 173 Y>S No Ensembl
rs731503203 174 V>G No Ensembl
rs738256463 198 L>R No Ensembl
rs732083534 199 T>A No Ensembl
rs741264589 201 M>L No Ensembl
rs733727526 202 L>R No Ensembl
rs737876280 215 C>R No Ensembl
rs730896566 247 T>P No Ensembl
rs741373133 256 I>T No Ensembl
rs736991015 328 W>G No Ensembl
rs736308298 329 D>G No Ensembl
rs739663479 334 S>G No Ensembl
rs738429396 345 S>P No Ensembl
rs741087558 346 Q>H No Ensembl
rs730947771 346 Q>P No Ensembl
rs735072434 374 V>G No Ensembl
rs739778892 375 R>G No Ensembl
rs737769601 377 M>V No Ensembl
rs731579686 409 T>P No Ensembl
rs740868575 415 S>P No Ensembl
rs734694470 417 T>P No Ensembl
rs732598908 420 R>G No Ensembl
rs737707079 434 E>G No Ensembl
rs735568913 440 D>A No Ensembl
rs733501777 445 K>Q No Ensembl

No associated diseases with Q8AYC9

4 regional properties for Q8AYC9

Type Name Position InterPro Accession
domain Forkhead-associated (FHA) domain 55 - 128 IPR000253
domain Protein kinase domain 200 - 480 IPR000719
active_site Serine/threonine-protein kinase, active site 324 - 336 IPR008271
binding_site Protein kinase, ATP binding site 206 - 229 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Chromosome
  • Cytoplasm
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
chromatin The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.
condensed nuclear chromosome A highly compacted molecule of DNA and associated proteins resulting in a cytologically distinct nuclear chromosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
histone kinase activity (H3-T11 specific) Catalysis of the transfer of a phosphate group to the threonine-11 residue of the N-terminal tail of histone H3.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.

14 GO annotations of biological process

Name Definition
cellular response to DNA damage stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.
DNA damage checkpoint signaling A signal transduction process that contributes to a DNA damage checkpoint.
DNA damage induced protein phosphorylation The widespread phosphorylation of various molecules, triggering many downstream processes, that occurs in response to the detection of DNA damage.
DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
epigenetic maintenance of chromatin in transcription-competent conformation An epigenetic process that capacitates gene expression by remodelling of chromatin by either modifying the chromatin fiber, the nucleosomal histones, or the DNA.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
mitotic G2 DNA damage checkpoint signaling A mitotic cell cycle checkpoint that detects and negatively regulates progression through the G2/M transition of the cell cycle in response to DNA damage.
negative regulation of mitotic nuclear division Any process that stops, prevents or reduces the rate or extent of mitosis. Mitosis is the division of the eukaryotic cell nucleus to produce two daughter nuclei that, usually, contain the identical chromosome complement to their mother.
peptidyl-threonine phosphorylation The phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of double-strand break repair via homologous recombination Any process that modulates the frequency, rate or extent of the error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequences.
regulation of histone H3-K9 acetylation Any process that modulates the frequency, rate or extent of histone H3-K9 acetylation.
regulation of mitotic centrosome separation Any process that modulates the frequency, rate or extent of the separation of duplicated centrosome components at the beginning of mitosis.
regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage Any process that modulates the frequency, rate or extent of transcription from an RNA polymerase II promoter as a result of a UV damage stimulus.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P28708 PRR1 Serine/threonine-protein kinase PRR1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P47116 PTK2 Serine/threonine-protein kinase PTK2/STK2 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
O61661 grp Serine/threonine-protein kinase grp Drosophila melanogaster (Fruit fly) SS
O14757 CHEK1 Serine/threonine-protein kinase Chk1 Homo sapiens (Human) EV
O35280 Chek1 Serine/threonine-protein kinase Chk1 Mus musculus (Mouse) PR
Q91ZN7 Chek1 Serine/threonine-protein kinase Chk1 Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MAVPFVEDWD LVQTLGEGAY GEVQLAVNRR TEEAVAVKIV DMKRAADCPE NIKKEICINK
70 80 90 100 110 120
MLNHENVVKF YGHRREGATQ YLFLEYCSGG ELFDRIEPDI GMPEPEAQRF FQQLIAGVVY
130 140 150 160 170 180
LHSMGITHRD LKPENLLLDE RDNLKISDFG LATVFKHNGR ERLLNKMCGT LPYVAPELLR
190 200 210 220 230 240
RPEFRAEPVD VWACGVVLTA MLAGELPWDQ PSDSCQEYSD WKERKTYLAP WRKIDSAPLA
250 260 270 280 290 300
LLHKILTENP TARITIPDIK KDRWYCRPLK KGTKRGRVSS GGVTESPGAL PKHIRSDTDF
310 320 330 340 350 360
SPVKSALGED KASYSTSQPE PGTGGALWDS STGSIDRLVQ GISFSQPACP EHMLLNSQLL
370 380 390 400 410 420
GTPGSSQSPW QRLVRRMTRF FTKLDADGSY RSLRDVCEKM GYGWKQSCTN QVTISTTDRR
430 440 450 460 470
NNKLIFKVNL LEMESRILVD FRLSKGDGLE FKRHFLKIKG KLSDVVSTQK VWLPPP