AiPD: Autoinhibited Protein Database

Q86VW2

Gene name	ARHGEF25 (GEFT)
Protein name	Rho guanine nucleotide exchange factor 25
Names	Guanine nucleotide exchange factor GEFT, Rac/Cdc42/Rho exchange factor GEFT, RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT, p63RhoGEF
Species	Homo sapiens (Human)
KEGG Pathway	hsa:115557
EC number
Protein Class	RHO GUANINE EXCHANGE FACTOR-RELATED (PTHR22826)

Descriptions

Dbl-family GEFs are the major class of exchange factors for Rho GTPases. The Dbl-family GEF p63RhoGEF, called ARHGEF25, is a mediator of Gαq/11 signaling to RhoA based on its ability to synergize with Gαq/11 resulting in enhanced RhoA signaling. Activated Gαq directly engages and stimulates ARHGEF25. Basically, ARHGEF25 is autoinhibited by the Dbl homology (DH)-associated pleckstrin homology (PH) domain. Activated Gαq relieves this autoinhibition by interacting with a highly conserved C-terminal extension of the PH domain.

Autoinhibitory domains (AIDs)

Target domain	155-347 (DH domain)
Relief mechanism	Partner binding
Assay	Deletion assay, Mutagenesis experiment, Structural analysis

AID

348-466 (PH domain)

Target domain

155-347 (DH domain)

Relief mechanism

Partner binding (Binding of activated Gαq to the C-terminal extension of the PH domain)

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Rojas RJ et al. (2007) "Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain", The Journal of biological chemistry, 282, 29201-10

Lutz S et al. (2007) "Structure of Galphaq-p63RhoGEF-RhoA complex reveals a pathway for the activation of RhoA by GPCRs", Science (New York, N.Y.), 318, 1923-7

Autoinhibited structure

Activated structure

2 structures for Q86VW2

Entry ID	Method	Resolution	Chain	Position	Source
2RGN	X-ray	350 A	B/E	149-502	PDB
AF-Q86VW2-F1	Predicted				AlphaFoldDB

466 variants for Q86VW2

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
CA385539375 rs1594958073	2	R>G		No	ClinGen Ensembl
CA385539381 rs60586224	2	R>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA6654103 rs60586224	2	R>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs60586224 CA6654102	2	R>Q		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs751372589 CA6654107	3	G>E		No	ClinGen ExAC TOPMed gnomAD
CA237810061 rs539901025	3	G>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA6654106 rs539901025	3	G>W		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs770018674 CA6654111	4	G>A		No	ClinGen ExAC TOPMed gnomAD
rs770018674 CA385539413	4	G>E		No	ClinGen ExAC TOPMed gnomAD
CA237810066 rs748042670	4	G>R		No	ClinGen ExAC TOPMed gnomAD
rs770018674 CA6654112	4	G>V		No	ClinGen ExAC TOPMed gnomAD
CA6654110 rs748042670	4	G>W		No	ClinGen ExAC TOPMed gnomAD
rs374654393 CA6654113	7	G>E		No	ClinGen ESP ExAC TOPMed gnomAD
CA385539527 rs1425569555	9	R>H		No	ClinGen gnomAD
rs1476701414 CA385539559	11	A>T		No	ClinGen gnomAD
CA385539617 rs1466619016	13	P>L		No	ClinGen gnomAD
rs866562446 CA237810072	14	R>H		No	ClinGen Ensembl
CA385539741 rs1565725857	18	K>Q		No	ClinGen Ensembl
CA6654118 rs772410817	19	V>L		No	ClinGen ExAC gnomAD
CA385539811 rs1472024007	21	A>T		No	ClinGen TOPMed
rs761119691 CA6654120	21	A>V		No	ClinGen ExAC gnomAD
rs909572575 CA237810081	24	G>C		No	ClinGen TOPMed
rs909572575 CA385539917	24	G>S		No	ClinGen TOPMed
rs941002090 CA237810083	24	G>V		No	ClinGen Ensembl
CA385540002 rs1594958193	25	C>W		No	ClinGen Ensembl
rs141989565 CA237810086	28	A>P		No	ClinGen TOPMed
CA385540092 rs141989565	28	A>S		No	ClinGen TOPMed
CA6654124 rs749987642	29	R>Q		No	ClinGen ExAC gnomAD
rs764755815 CA6654123	29	R>W		No	ClinGen ExAC TOPMed
CA385540170 rs1223901092	30	G>V		No	ClinGen TOPMed
rs1277199432 CA385540157	30	G>W		No	ClinGen gnomAD
CA6654126 rs766016732	31	G>R		No	ClinGen ExAC gnomAD
CA385540219 rs1322353675	31	G>V		No	ClinGen TOPMed
CA385540234 rs1237013003	32	R>H		No	ClinGen TOPMed
CA385540235 rs1237013003	32	R>L		No	ClinGen TOPMed
rs927689400 CA237810094	32	R>S		No	ClinGen TOPMed gnomAD
CA385541426 rs1193170599	34	S>F		No	ClinGen gnomAD
rs759188636 CA6654147	34	S>T		No	ClinGen ExAC gnomAD
CA6654148 rs144451436	35	Y>C		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs936283782 CA237810310	36	S>F		No	ClinGen TOPMed
rs1179265081 CA385541461	37	I>F		No	ClinGen gnomAD
rs200472423 CA6654150	38	A>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA6654149 rs200472423	38	A>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs139793201 CA6654153	41	E>K		No	ClinGen ESP ExAC gnomAD
rs1412663943 CA385541522	42	G>R		No	ClinGen TOPMed
CA6654155 rs746105629	44	I>M		No	ClinGen ExAC
rs1594959035 CA385541551	44	I>V		No	ClinGen Ensembl
CA6654156 rs376912565	45	S>L		No	ClinGen ESP ExAC TOPMed gnomAD
CA6654157 rs780245693	47	S>F		No	ClinGen ExAC gnomAD
rs1310888018 CA385541598	48	A>S		No	ClinGen gnomAD
rs529397542 CA6654158	49	A>T		No	ClinGen 1000Genomes ExAC gnomAD
rs1313286392 CA385541627	50	S>F		No	ClinGen gnomAD
rs768946799 CA6654159	50	S>P		No	ClinGen ExAC TOPMed gnomAD
COSM1512825 COSM1512824 rs369276230 CA6654162	51	G>C	lung [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA6654161 rs369276230	51	G>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA385541673 rs1468260729	54	A>D		No	ClinGen gnomAD
rs1468260729 CA385541675	54	A>V		No	ClinGen gnomAD
CA6654166 rs151122035	55	P>H		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA6654167 rs151122035	55	P>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs151122035 CA6654164	55	P>R		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA385541783 rs1425941614	63	S>R		No	ClinGen gnomAD
CA385541780 rs1415098293	63	S>T		No	ClinGen gnomAD
CA6654169 rs775275007	66	P>A		No	ClinGen ExAC TOPMed gnomAD
CA6654170 rs377531308	67	C>S		No	ClinGen ESP ExAC TOPMed gnomAD
rs1332916743 CA385541857	69	P>Q		No	ClinGen gnomAD
rs763923142 CA6654171	69	P>S		No	ClinGen ExAC gnomAD
rs753845308 CA6654172	70	G>S		No	ClinGen ExAC TOPMed gnomAD
rs533686624 CA6654174	71	P>S		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs750562465 CA6654175	72	P>Q		No	ClinGen ExAC TOPMed gnomAD
rs551752562 CA6654176	73	G>A		No	ClinGen 1000Genomes ExAC gnomAD
rs551752562 CA385541909	73	G>E		No	ClinGen 1000Genomes ExAC gnomAD
CA6654179 rs140959163	75	V>I		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA6654180 rs781496619	76	S>C		No	ClinGen ExAC gnomAD
rs1260194034 CA385541966	77	G>D		No	ClinGen gnomAD
rs748430628 CA6654181	78	L>Q		No	ClinGen ExAC gnomAD
CA385541991 rs1352426065	79	R>K		No	ClinGen gnomAD
rs770383336 CA6654182	80	R>K		No	ClinGen ExAC TOPMed gnomAD
rs770383336 CA6654183	80	R>T		No	ClinGen ExAC TOPMed gnomAD
rs1436539191 CA385542031	82	L>S		No	ClinGen TOPMed
CA385542027 rs1238120392	82	L>V		No	ClinGen TOPMed gnomAD
rs199703624 CA6654184	83	D>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
COSM107020 rs150735183 CA237810352	85	S>F	skin [Cosmic]	No	ClinGen cosmic curated Ensembl
rs979136030 CA237810350	85	S>P		No	ClinGen Ensembl
CA385542090 rs1325767391	87	H>Y		No	ClinGen TOPMed
rs369675789 CA385542118	88	C>F		No	ClinGen ESP ExAC TOPMed gnomAD
COSM1363380 COSM1363381 rs771640862 CA6654185	88	C>G	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs369675789 CA6654186	88	C>Y		No	ClinGen ESP ExAC TOPMed gnomAD
CA237810358 rs926144701	90	S>T		No	ClinGen Ensembl
rs1420944941 CA385542223	95	A>T		No	ClinGen gnomAD
CA6654188 rs145779061	96	D>E		No	ClinGen ESP ExAC TOPMed gnomAD
CA385542236 rs1464651792	96	D>N		No	ClinGen gnomAD
CA385542277 rs1594959310	98	G>S		No	ClinGen Ensembl
CA385542308 rs1417595663	99	Q>H		No	ClinGen TOPMed
CA237810363 rs921491978	100	A>T		No	ClinGen TOPMed gnomAD
rs531612180 CA237810366	100	A>V		No	ClinGen Ensembl
rs1445801462 CA385542349	101	G>E		No	ClinGen gnomAD
rs1445801462 CA385542351	101	G>V		No	ClinGen gnomAD
CA6654190 rs761488499	102	P>S		No	ClinGen ExAC TOPMed gnomAD
CA385542404 rs1340929966	104	E>K		No	ClinGen gnomAD
CA6654206 rs776131100	106	W>C		No	ClinGen ExAC TOPMed gnomAD
rs1038444571 CA237810390	111	A>T		No	ClinGen Ensembl
rs773228709 CA6654209	116	E>K		No	ClinGen ExAC TOPMed gnomAD
rs773228709 CA237810398	116	E>Q		No	ClinGen ExAC TOPMed gnomAD
CA6654211 rs766273748	117	E>K		No	ClinGen ExAC gnomAD
CA6654212 rs201573584	118	L>M		No	ClinGen 1000Genomes ExAC
CA6654215 rs140645509	119	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
CA6654214 rs767849431	119	P>T		No	ClinGen ExAC gnomAD
rs1594959482 CA385542740	120	E>K		No	ClinGen Ensembl
rs1192390644 CA385542751	121	L>P		No	ClinGen gnomAD
CA385542765 rs1565726678	122	T>S		No	ClinGen Ensembl
rs757876691 CA6654219	128	L>S		No	ClinGen ExAC TOPMed gnomAD
rs1475675956 CA385542850	129	E>G		No	ClinGen gnomAD
CA385542866 rs1384011904	130	G>C		No	ClinGen gnomAD
rs1405689214 CA385542873	130	G>V		No	ClinGen gnomAD
CA6654222 rs754473725	132	G>*		No	ClinGen ExAC gnomAD
CA237810419 rs780840561	133	D>E		No	ClinGen TOPMed
CA385542918 rs1434570805	133	D>N		No	ClinGen gnomAD
COSM1363383 rs138453741 CA6654223 COSM1363382	135	T>M	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA385542983 rs577533598	136	Q>*		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA6654225 rs577533598	136	Q>K		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs143068244 CA6654241	138	P>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs544973692 CA6654240	138	P>S		No	ClinGen ExAC TOPMed gnomAD
CA237810447 rs868770859	141	E>*		No	ClinGen Ensembl
CA237810449 rs368208007	141	E>D		No	ClinGen ESP TOPMed
rs747667238 CA6654242	142	T>I		No	ClinGen ExAC gnomAD
rs777469251 CA6654245	144	S>F		No	ClinGen ExAC TOPMed
COSM1363384 COSM1363385 rs755771922 CA6654243	144	S>P	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
CA385543308 rs1565726783	145	Q>E		No	ClinGen Ensembl
rs1315643782 CA385543355	146	A>D		No	ClinGen TOPMed
CA385543417 rs1565726789	148	E>D		No	ClinGen Ensembl
CA6654247 rs749148752	149	S>I		No	ClinGen ExAC gnomAD
CA6654249 rs770647857	154	K>N		No	ClinGen ExAC TOPMed gnomAD
rs1189502842 CA385543629	155	K>*		No	ClinGen TOPMed gnomAD
rs1189502842 CA385543633	155	K>Q		No	ClinGen TOPMed gnomAD
CA237810468 rs894952649	156	K>E		No	ClinGen TOPMed gnomAD
CA237810470 rs947921217	156	K>T		No	ClinGen TOPMed gnomAD
CA6654251 rs561061153	157	A>G		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA237810473 rs898453360	157	A>T		No	ClinGen TOPMed
CA6654252 rs561061153	157	A>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA6654253 rs775468569	160	R>G		No	ClinGen ExAC gnomAD
CA6654272 rs780023911	162	M>I		No	ClinGen ExAC TOPMed gnomAD
CA385543827 rs1165254996	162	M>L		No	ClinGen TOPMed
rs1565726843 CA385543852	162	M>T		No	ClinGen Ensembl
rs527974498 CA6654274	163	Y>*		No	ClinGen 1000Genomes ExAC gnomAD
CA6654273 rs747122399	163	Y>C		No	ClinGen ExAC TOPMed gnomAD
CA385544009 rs1331203984	163	Y>N		No	ClinGen gnomAD
CA6654275 rs777018043	166	S>N		No	ClinGen ExAC TOPMed gnomAD
CA6654276 rs762090159	167	E>K		No	ClinGen ExAC gnomAD
CA385544201 rs1333168249	171	T>A		No	ClinGen TOPMed
rs773765910 CA6654279	174	M>T		No	ClinGen ExAC gnomAD
rs770113774 CA6654278	174	M>V		No	ClinGen ExAC TOPMed gnomAD
CA385544356 rs1433843689	176	V>L		No	ClinGen TOPMed gnomAD
CA385544354 rs1433843689	176	V>M		No	ClinGen TOPMed gnomAD
rs61935728 COSM268700 CA237810535	178	D>N	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed
rs61935728 CA385544410	178	D>Y		No	ClinGen TOPMed
rs147980194 CA6654283	181	Q>K		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA385544461 rs1316851265	181	Q>L		No	ClinGen TOPMed gnomAD
rs1565727148 CA385544732	187	M>T		No	ClinGen Ensembl
rs758139060 CA6654306	190	M>I		No	ClinGen ExAC
CA6654307 rs766160305	191	A>T		No	ClinGen ExAC gnomAD
rs1341956486 CA385544832	192	A>S		No	ClinGen TOPMed
CA6654308 rs369247314	192	A>V		No	ClinGen ESP ExAC TOPMed gnomAD
rs1429355540 CA385544854	193	Q>P		No	ClinGen TOPMed
CA6654309 rs755018413	194	G>R		No	ClinGen ExAC TOPMed gnomAD
CA385544886 rs1594960352	195	V>G		No	ClinGen Ensembl
CA237810621 rs373514799	196	P>R		No	ClinGen ESP TOPMed gnomAD
rs1463355923 CA385544932	197	E>D		No	ClinGen TOPMed
rs902398268 CA237810623	197	E>K		No	ClinGen Ensembl
rs1177118328 CA385544944	198	S>N		No	ClinGen TOPMed gnomAD
CA6654311 rs748306195	198	S>R		No	ClinGen ExAC TOPMed gnomAD
COSM549364 rs1474370014 CA385544976 COSM1140232	200	R>*	lung [Cosmic]	No	ClinGen cosmic curated TOPMed
rs756197273 COSM1253174 CA6654313 COSM1253173	200	R>Q	oesophagus large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC gnomAD
rs1416622579 CA385544999	201	G>D		No	ClinGen gnomAD
CA385545016 rs1465810929	202	R>C		No	ClinGen gnomAD
rs777873296 CA6654314	204	R>K		No	ClinGen ExAC gnomAD
CA6654316 rs771228784	211	Q>H		No	ClinGen ExAC gnomAD
rs774815529 CA6654317	214	Y>C		No	ClinGen ExAC TOPMed gnomAD
rs1565727218 CA385545389 COSM694938 COSM1147081	217	H>Y	lung [Cosmic]	No	ClinGen cosmic curated Ensembl
CA6654320 rs141643619	219	D>N		No	ClinGen ESP ExAC TOPMed gnomAD
CA237810642 rs141643619	219	D>Y		No	ClinGen ESP ExAC TOPMed gnomAD
CA6654341 rs532231407	220	Y>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA385545650 rs1356502299	223	Q>R		No	ClinGen TOPMed
CA6654344 rs150532634	226	Q>L		No	ClinGen ESP TOPMed
rs150532634 CA6654343	226	Q>R		No	ClinGen ESP TOPMed
CA6654347 rs547082119	227	R>Q		No	ClinGen 1000Genomes ExAC gnomAD
CA6654346 rs377474244	227	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
rs1474798686 CA385545819	231	D>E		No	ClinGen gnomAD
rs759421652 CA6654349	232	P>S		No	ClinGen ExAC TOPMed gnomAD
CA237810725 rs951176649	234	W>*		No	ClinGen TOPMed
CA237810727 rs951176649	234	W>C		No	ClinGen TOPMed
CA385545895 rs1396348759	235	L>V		No	ClinGen gnomAD
CA6654350 rs767374507	236	A>G		No	ClinGen ExAC gnomAD
CA237810731 rs974306796	236	A>T		No	ClinGen Ensembl
CA6654351 rs752715781	238	L>V		No	ClinGen ExAC TOPMed gnomAD
rs1378382036 CA385546195	243	E>V		No	ClinGen gnomAD
CA385546226 rs1278195704	244	R>C		No	ClinGen gnomAD
rs1350372396 CA385546231	244	R>H		No	ClinGen gnomAD
CA385546215 rs1278195704	244	R>S		No	ClinGen gnomAD
rs1259114445 CA385546302	248	M>L		No	ClinGen TOPMed
CA6654380 rs374554426	249	Y>H		No	ClinGen ESP ExAC TOPMed gnomAD
CA237810850 rs900217679	253	C>G		No	ClinGen Ensembl
CA6654382 rs17857333	253	C>S		No	ClinGen ExAC TOPMed
CA237810853 VAR_039402 rs17857333	253	C>Y		No	ClinGen UniProt ExAC TOPMed dbSNP
CA385546459 rs1565727456	255	N>S		No	ClinGen Ensembl
rs1238235190 CA385546471	256	K>*		No	ClinGen gnomAD
rs748559108 CA6654383	257	P>S		No	ClinGen ExAC TOPMed gnomAD
rs756826304 CA6654384	258	K>T		No	ClinGen ExAC gnomAD
rs545434777 CA6654388	262	V>M		No	ClinGen 1000Genomes ExAC gnomAD
rs1404632656 CA385548556	264	S>P		No	ClinGen gnomAD
rs1412171634 CA385548648	270	Y>F		No	ClinGen gnomAD
rs1417038425 CA385548728	273	E>D		No	ClinGen TOPMed
rs866129970 CA237810910	273	E>K		No	ClinGen gnomAD
CA237810913 rs965201958	274	L>V		No	ClinGen TOPMed
rs1373751027 CA385548746	275	R>Q		No	ClinGen gnomAD
rs565431564 CA6654403	275	R>W		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs878927141 CA237810916	276	Q>R		No	ClinGen Ensembl
rs116792227 CA385548773	277	Q>H		No	ClinGen 1000Genomes gnomAD
CA237810918 rs116792227	277	Q>H		No	ClinGen 1000Genomes gnomAD
rs753076748 CA6654404	278	L>V		No	ClinGen ExAC gnomAD
rs756625592 CA6654405	279	G>V		No	ClinGen ExAC gnomAD
rs1485139707 CA385548801	280	H>L		No	ClinGen TOPMed
rs1485139707 CA385548805	280	H>P		No	ClinGen TOPMed
CA6654407 rs745434149	281	R>C		No	ClinGen ExAC gnomAD
CA385548818 rs1373989149	281	R>H		No	ClinGen gnomAD
CA385548819 rs1373989149	281	R>P		No	ClinGen gnomAD
rs1413819587 CA385548837	283	Q>K		No	ClinGen gnomAD
rs995795606 CA237810925	285	N>K		No	ClinGen gnomAD
rs757911470 CA6654408	285	N>S		No	ClinGen ExAC gnomAD
rs779865792 CA6654409	287	L>F		No	ClinGen ExAC gnomAD
rs547436770 CA237810931	288	L>V		No	ClinGen 1000Genomes TOPMed
rs746592951 CA6654411	289	I>T		No	ClinGen ExAC gnomAD
CA385548942 rs1212907781	291	P>H		No	ClinGen gnomAD
CA385548943 rs1212907781	291	P>R		No	ClinGen gnomAD
CA237810944 rs1027230570	294	R>Q		No	ClinGen gnomAD
rs768526101 CA6654412	294	R>W		No	ClinGen ExAC TOPMed gnomAD
rs776329504 CA6654413	295	I>T		No	ClinGen ExAC TOPMed gnomAD
rs1176031480 CA385549090	303	K>N		No	ClinGen gnomAD
rs1454216761 CA385549161	305	F>V		No	ClinGen gnomAD
CA385549189 rs1160362678	306	L>I		No	ClinGen gnomAD
rs754646825 CA6654432	308	Y>C		No	ClinGen ExAC gnomAD
rs965146652 CA237811057	310	N>D		No	ClinGen TOPMed
COSM1512818 rs1424455112 COSM1512819 CA385549289	310	N>S	lung [Cosmic]	No	ClinGen cosmic curated gnomAD
rs1157593756 CA385549302	311	R>T		No	ClinGen gnomAD
CA385549314 rs1448254356	312	A>T		No	ClinGen TOPMed
CA385549324 rs1565727756	312	A>V		No	ClinGen Ensembl
CA385549359 rs1394611832	314	M>T		No	ClinGen gnomAD
CA6654433 rs570006492	315	D>E		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs747900629 CA6654434	316	T>I		No	ClinGen ExAC gnomAD
rs747900629 CA237811066	316	T>N		No	ClinGen ExAC gnomAD
CA385549423 rs1391888500	317	A>E		No	ClinGen TOPMed gnomAD
CA6654435 rs769521282	317	A>T		No	ClinGen ExAC gnomAD
CA385549427 rs1391888500	317	A>V		No	ClinGen TOPMed gnomAD
CA6654449 rs751098474	321	Q>K		No	ClinGen ExAC gnomAD
CA385549559 rs1205861847	322	A>D		No	ClinGen gnomAD
CA6654450 rs754446081	324	E>D		No	ClinGen ExAC gnomAD
CA385549579 rs1253539267	324	E>K		No	ClinGen gnomAD
rs780721321 CA6654451	327	C>G		No	ClinGen ExAC gnomAD
rs989235653 CA237811167	329	V>A		No	ClinGen gnomAD
CA237811180 rs755574255	332	R>C		No	ClinGen TOPMed gnomAD
rs755574255 CA237811169	332	R>G		No	ClinGen TOPMed gnomAD
COSM294138 CA6654452 rs752298345	332	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated ExAC TOPMed gnomAD
rs1352758268 CA385549751	335	D>N		No	ClinGen gnomAD
rs749063493 CA6654456	336	M>I		No	ClinGen ExAC TOPMed gnomAD
CA6654455 rs749063493	336	M>I		No	ClinGen ExAC TOPMed gnomAD
rs777588792 CA6654454	336	M>L		No	ClinGen ExAC gnomAD
rs1476308162 CA385549791	337	M>L		No	ClinGen TOPMed
rs542655828 CA6654457	338	T>M		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs1291616071 CA385549877	340	G>R		No	ClinGen gnomAD
CA385549916 rs1378551862	341	R>I		No	ClinGen gnomAD
rs760938850 CA6654461	343	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA6654460 rs147406368	343	R>W		No	ClinGen ESP ExAC TOPMed gnomAD
rs554543726 CA6654462	344	G>E		No	ClinGen 1000Genomes ExAC gnomAD
CA385549962 rs554543726	344	G>V		No	ClinGen 1000Genomes ExAC gnomAD
CA6654463 rs777183338	346	E>G		No	ClinGen ExAC gnomAD
rs1452499132 CA385550123	348	K>Q		No	ClinGen gnomAD
CA6654482 rs139724074	351	A>D		No	ClinGen ESP ExAC TOPMed gnomAD
CA385550217 rs139724074	351	A>G		No	ClinGen ESP ExAC TOPMed gnomAD
CA385550192 rs1171624787	351	A>T		No	ClinGen gnomAD
CA237811283 rs1041053842	352	Q>K		No	ClinGen TOPMed
rs773625202 CA6654483	352	Q>L		No	ClinGen ExAC TOPMed gnomAD
rs773625202 CA385550239	352	Q>R		No	ClinGen ExAC TOPMed gnomAD
CA385550253 rs1448817776	353	G>V		No	ClinGen TOPMed
CA6654484 rs763559683	354	K>E		No	ClinGen ExAC
CA237811303 rs1025223101	355	L>P		No	ClinGen Ensembl
CA237811310 rs906562147	358	Q>E		No	ClinGen Ensembl
rs1594961976 CA385550558	364	T>P		No	ClinGen Ensembl
rs760177394 CA6654487	365	E>K		No	ClinGen ExAC TOPMed gnomAD
rs1308863835 CA385550635	367	E>V		No	ClinGen TOPMed
CA385550666 rs1273155882	369	G>A		No	ClinGen TOPMed
CA6654490 rs757021330	375	R>*		No	ClinGen ExAC TOPMed gnomAD
rs373576910 CA6654491	375	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
CA385550824 rs1281629612	376	G>V		No	ClinGen gnomAD
rs1293298145 CA385550843	377	R>*		No	ClinGen TOPMed
rs375478212 CA6654493	377	R>L		No	ClinGen ESP ExAC TOPMed gnomAD
rs375478212 CA385550846	377	R>Q		No	ClinGen ESP ExAC TOPMed gnomAD
CA385550884 rs1467122427	378	E>D		No	ClinGen gnomAD
rs780124033 CA6654494	378	E>G		No	ClinGen ExAC gnomAD
rs79131014 CA6654496	380	R>C		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA237811363 COSM1363393 COSM1363392 rs1029273163	380	R>H	large_intestine [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs369900388 CA6654497 COSM3688373 COSM3688374	381	V>I	large_intestine [Cosmic]	No	ClinGen cosmic curated ESP ExAC TOPMed gnomAD
CA6654498 rs748362162	385	E>*		No	ClinGen ExAC TOPMed gnomAD
rs1396747504 CA385551060	385	E>D		No	ClinGen TOPMed gnomAD
CA385551063 rs1396747504	385	E>D		No	ClinGen TOPMed gnomAD
CA385551118 rs1481423058	388	I>V		No	ClinGen TOPMed
rs1404126919 CA385551135	389	I>V		No	ClinGen gnomAD
rs200042882 CA6654499	392	E>G		No	ClinGen 1000Genomes ExAC gnomAD
rs17854492 VAR_039403 CA237811380	397	G>R		No	ClinGen UniProt Ensembl dbSNP
CA385551399 rs1336739484	398	V>M		No	ClinGen gnomAD
rs1233057398 CA385551440	399	R>I		No	ClinGen TOPMed
CA385551455 rs1444733437	400	G>R		No	ClinGen TOPMed gnomAD
rs1278144259 CA385551536	403	Q>*		No	ClinGen TOPMed gnomAD
rs987825856 CA237811383	403	Q>R		No	ClinGen gnomAD
CA6654500 rs142280351	404	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA385551627 rs1237227344	405	G>V		No	ClinGen TOPMed
CA385551668 rs1313264484	407	V>A		No	ClinGen TOPMed
rs771537977 CA6654502	408	Y>F		No	ClinGen ExAC gnomAD
CA385551678 rs1257043627	408	Y>H		No	ClinGen TOPMed gnomAD
rs151266618 CA6654504	410	N>K		No	ClinGen ESP ExAC gnomAD
rs1273563890 CA385551795	413	K>E		No	ClinGen gnomAD
CA385551914 rs1396636714	414	V>L		No	ClinGen TOPMed
CA237811452 rs948238996	415	S>N		No	ClinGen Ensembl
rs1440897750 CA385551989	416	C>S		No	ClinGen gnomAD
CA385552032 rs1373684608	418	G>*		No	ClinGen gnomAD
rs1471059559 CA385552135	421	G>E		No	ClinGen TOPMed gnomAD
rs370412283 CA6654521	421	G>R		No	ClinGen ESP ExAC TOPMed gnomAD
CA385552190 rs1167204700	423	L>I		No	ClinGen TOPMed gnomAD
CA6654523 rs746331043	423	L>P		No	ClinGen ExAC TOPMed gnomAD
CA6654525 rs565885022	424	Q>*		No	ClinGen 1000Genomes ExAC gnomAD
CA6654524 rs565885022	424	Q>E		No	ClinGen 1000Genomes ExAC gnomAD
CA385552269 rs1315168319	425	G>S		No	ClinGen gnomAD
rs761212971 CA385552294	426	D>E		No	ClinGen ExAC TOPMed gnomAD
rs1395062477 CA385552282	426	D>N		No	ClinGen gnomAD
rs749400140 CA6654527	427	P>T		No	ClinGen ExAC TOPMed gnomAD
rs1432852769 CA385552325	428	C>R		No	ClinGen gnomAD
CA6654529 rs762829220	429	R>C		No	ClinGen ExAC TOPMed gnomAD
CA385552353 rs762829220	429	R>G		No	ClinGen ExAC TOPMed gnomAD
CA6654530 rs766237649	429	R>H		No	ClinGen ExAC TOPMed gnomAD
CA6654531 rs751370716	431	A>V		No	ClinGen ExAC TOPMed gnomAD
rs759599421 CA6654533	432	L>V		No	ClinGen ExAC gnomAD
CA385552531 rs1269216994	435	R>I		No	ClinGen TOPMed
CA385552536 rs1208448297	436	G>R		No	ClinGen TOPMed
rs1262541164 CA385552548	437	P>S		No	ClinGen gnomAD
CA385552579 rs1187229812	439	G>D		No	ClinGen gnomAD
rs1327378426 CA385552620	442	Q>R		No	ClinGen TOPMed
RCV001261750 rs778066908 CA6654537	443	R>C		No	ClinGen ClinVar ExAC dbSNP gnomAD
CA237811488 rs1030933223	443	R>H		No	ClinGen TOPMed gnomAD
rs1030933223 CA385552637	443	R>L		No	ClinGen TOPMed gnomAD
CA6654538 rs778066908	443	R>S		No	ClinGen ExAC gnomAD
CA385552654 rs1386501253	444	Y>*		No	ClinGen TOPMed gnomAD
CA6654540 rs372798341	444	Y>C		No	ClinGen ESP ExAC TOPMed gnomAD
rs1465255981 CA385552640	444	Y>H		No	ClinGen gnomAD
rs1345808773 CA385552662	445	V>A		No	ClinGen TOPMed gnomAD
rs772623460 CA6654542	447	Q>*		No	ClinGen ExAC gnomAD
rs780420723 CA6654543	447	Q>L		No	ClinGen ExAC gnomAD
rs747697380 CA6654544	449	A>P		No	ClinGen ExAC gnomAD
CA237811493 rs1001249025	450	D>E		No	ClinGen TOPMed
rs1366246152 CA385552876	453	I>V		No	ClinGen TOPMed
rs370713793 CA6654546	454	S>R		No	ClinGen ESP ExAC gnomAD
rs1232845652 CA385552914	454	S>T		No	ClinGen gnomAD
rs760735071 CA237811496	455	Q>R		No	ClinGen Ensembl
CA385553006 rs1331332546	457	W>*		No	ClinGen gnomAD
CA6654547 rs762737367	458	I>F		No	ClinGen ExAC gnomAD
CA385553058 rs770686201	458	I>M		No	ClinGen ExAC gnomAD
rs189229272 CA6654549	459	K>R		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA385553151 rs1594962609	460	H>P		No	ClinGen Ensembl
CA6654550 rs759403266	460	H>Q		No	ClinGen ExAC gnomAD
rs201365302 CA6654552	462	A>V		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
CA6654553 rs760827052	467	S>N		No	ClinGen ExAC gnomAD
rs753921549 CA6654555	469	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA6654554 rs764036012	469	R>W		No	ClinGen ExAC TOPMed gnomAD
rs150749682 CA6654557	473	N>D		No	ClinGen 1000Genomes ExAC gnomAD
rs1308879969 CA385553524	474	A>T		No	ClinGen TOPMed gnomAD
CA385553771 rs1484019777	476	Q>R		No	ClinGen gnomAD
CA385553852 rs1223883211	478	P>S		No	ClinGen TOPMed gnomAD
rs755656909 CA6654581	479	I>T		No	ClinGen ExAC gnomAD
rs1429074041 CA385554009	481	Y>*		No	ClinGen gnomAD
CA237811658 rs1050942155	483	R>I		No	ClinGen Ensembl
CA385554105 rs766056217	484	R>L		No	ClinGen ExAC TOPMed gnomAD
CA6654583 rs766056217	484	R>Q		No	ClinGen ExAC TOPMed gnomAD
CA6654582 rs751932441	484	R>W		No	ClinGen ExAC TOPMed gnomAD
rs1594962930 CA385554164	486	S>G		No	ClinGen Ensembl
CA6654584 rs781721813	489	N>K		No	ClinGen ExAC gnomAD
CA385554268 rs1416045502	489	N>S		No	ClinGen TOPMed
CA6654585 rs748815135	491	L>P		No	ClinGen ExAC gnomAD
rs1293037090 CA385554381	492	G>E		No	ClinGen gnomAD
rs116051216 CA385554433	493	R>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs116051216 CA6654587 COSM171369	493	R>Q	large_intestine [Cosmic]	No	ClinGen cosmic curated 1000Genomes ESP ExAC TOPMed gnomAD
rs756773745 CA6654586	493	R>W		No	ClinGen ExAC TOPMed gnomAD
rs371753918 CA6654589	494	P>T		No	ClinGen ESP ExAC TOPMed gnomAD
rs79529064 CA6654590	496	G>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs375345701 CA237811690	497	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA6654591 rs375345701	497	P>T		No	ClinGen ESP ExAC TOPMed gnomAD
COSM1476790 COSM431599 CA385554577 rs1228199341	498	G>A	breast [Cosmic]	No	ClinGen cosmic curated TOPMed gnomAD
rs949857766 CA385554583	499	V>L		No	ClinGen TOPMed gnomAD
rs949857766 CA237811691	499	V>M		No	ClinGen TOPMed gnomAD
rs369392393 CA6654593	501	S>G		No	ClinGen ESP ExAC TOPMed gnomAD
CA6654594 rs115485566	501	S>R		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs536365007 CA6654595	504	R>K		No	ClinGen 1000Genomes ExAC gnomAD
CA385554806 rs1190449783	505	I>M		No	ClinGen gnomAD
rs773245828 CA6654596	506	Q>*		No	ClinGen ExAC gnomAD
CA6654598 rs766612522	506	Q>H		No	ClinGen ExAC gnomAD
rs1564374 CA237811714	506	Q>L		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
CA385554818 rs1564374	506	Q>P		No	ClinGen 1000Genomes ESP ExAC TOPMed gnomAD
rs1564374 VAR_039404 CA6654597	506	Q>R		No	ClinGen UniProt 1000Genomes ESP ExAC TOPMed dbSNP gnomAD
CA6654599 rs150264977	510	Q>R		No	ClinGen ESP ExAC TOPMed gnomAD
rs1027565197 CA237811716	511	A>T		No	ClinGen TOPMed
rs759881337 CA6654602	511	A>V		No	ClinGen ExAC gnomAD
rs768155733 CA6654603	513	G>C		No	ClinGen ExAC gnomAD
CA6654604 rs768155733	513	G>S		No	ClinGen ExAC gnomAD
rs115150056 CA6654607	515	T>K		No	ClinGen 1000Genomes ExAC TOPMed gnomAD
rs749927530 CA6654609	516	H>P		No	ClinGen ExAC TOPMed gnomAD
CA385555175 rs1370132779	518	P>A		No	ClinGen TOPMed gnomAD
CA385555182 rs1283058037	518	P>H		No	ClinGen TOPMed
CA385555188 rs1283058037	518	P>R		No	ClinGen TOPMed
CA385555176 rs1370132779	518	P>S		No	ClinGen TOPMed gnomAD
rs758040973 CA6654610	520	N>D		No	ClinGen ExAC TOPMed gnomAD
rs779751627 CA6654611	520	N>S		No	ClinGen ExAC gnomAD
rs1323007014 CA385555262	521	G>V		No	ClinGen TOPMed
rs199891478 CA6654612	522	S>C		No	ClinGen ExAC gnomAD
rs199891478 CA237811750	522	S>F		No	ClinGen ExAC gnomAD
rs1388200939 CA385555307	523	L>F		No	ClinGen TOPMed
rs759557573 CA6654613	524	P>H		No	ClinGen ExAC gnomAD
CA6654614 rs781129004	525	S>C		No	ClinGen ExAC gnomAD
rs1157287337 CA385555440	528	L>R		No	ClinGen TOPMed
CA385555482 rs1476879481	530	P>L		No	ClinGen gnomAD
CA385555495 rs1565728867	531	K>E		No	ClinGen Ensembl
CA385555599 rs1440230400	534	V>A		No	ClinGen Ensembl
rs748125313 CA6654618	535	A>D		No	ClinGen ExAC gnomAD
rs1161510073 CA385555686	540	P>T		No	ClinGen gnomAD
rs1565728893 CA385555749	543	K>E		No	ClinGen Ensembl
CA6654620 rs773340260	544	Q>*		No	ClinGen ExAC TOPMed gnomAD
CA385557096 rs1354209303	546	L>R		No	ClinGen gnomAD
rs749343142 CA6654639	547	G>D		No	ClinGen ExAC gnomAD
CA385557137 rs1180761727	548	D>N		No	ClinGen TOPMed
CA6654641 rs774472264	550	P>R		No	ClinGen ExAC gnomAD
rs1207581636 CA385557208	550	P>S		No	ClinGen gnomAD
rs1207581636 CA385557204	550	P>T		No	ClinGen gnomAD
CA385557232 rs1289593909	551	Q>L		No	ClinGen gnomAD
CA6654644 rs776051869	552	A>S		No	ClinGen ExAC gnomAD
CA385557258 rs370774821	553	P>S		No	ClinGen ESP ExAC TOPMed gnomAD
CA6654646 rs370774821	553	P>T		No	ClinGen ESP ExAC TOPMed gnomAD
CA385557279 rs1594963694	555	D>A		No	ClinGen Ensembl
rs1163143985 CA385557303	557	P>A		No	ClinGen gnomAD
rs981198557 CA237811909	558	P>L		No	ClinGen Ensembl
rs1206887266 CA385557313	558	P>S		No	ClinGen TOPMed gnomAD
CA6654647 rs777137013	560	S>Y		No	ClinGen ExAC gnomAD
rs751012060 CA6654650	562	T>N		No	ClinGen ExAC
CA6654649 rs765873004	562	T>P		No	ClinGen ExAC
CA385557390 rs1167084712	563	P>S		No	ClinGen gnomAD
rs752496393 CA6654654	564	K>E		No	ClinGen ExAC gnomAD
CA6654655 rs755847425	565	T>P		No	ClinGen ExAC TOPMed gnomAD
rs1338154582 CA385557458	567	P>H		No	ClinGen TOPMed gnomAD
rs1594963788 CA385557466	568	C>G		No	ClinGen Ensembl
rs757115464 CA6654660	569	Q>K		No	ClinGen ExAC
rs1031346385 CA237811966	570	A>G		No	ClinGen TOPMed gnomAD
rs779094951 CA6654661	570	A>T		No	ClinGen ExAC TOPMed gnomAD
CA385557508 rs1031346385	570	A>V		No	ClinGen TOPMed gnomAD
rs988243738 CA237811977	571	R>I		No	ClinGen TOPMed gnomAD
rs200996964 CA6654664	572	L>F		No	ClinGen 1000Genomes ExAC gnomAD
CA6654665 rs747388856	572	L>H		No	ClinGen ExAC gnomAD
rs1187477677 CA385557563	574	K>E		No	ClinGen TOPMed gnomAD
rs777034041 CA6654667	574	K>N		No	ClinGen ExAC gnomAD
rs1389917371 CA385557603	576	D>E		No	ClinGen gnomAD
rs1187106449 CA385557596	576	D>V		No	ClinGen gnomAD
COSM1476791 rs915505248 CA237811984 COSM431600	577	E>K	breast [Cosmic]	No	ClinGen cosmic curated Ensembl
rs1458507021 CA385557681	581	L>Q		No	ClinGen TOPMed

No associated diseases with Q86VW2

2 regional properties for Q86VW2

Type	Name	Position	InterPro Accession
domain	Dbl homology (DH) domain	160 - 336	IPR000219
domain	Pleckstrin homology domain	348 - 466	IPR001849

Functions

		Description
EC Number
Subcellular Localization	Cell membrane Cytoplasm, myofibril, sarcomere	Highly colocalizes with actin regions
PANTHER Family	PTHR22826	RHO GUANINE EXCHANGE FACTOR-RELATED
PANTHER Subfamily	PTHR22826:SF117	PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 4B-RELATED
PANTHER Protein Class	guanyl-nucleotide exchange factor G-protein modulator
PANTHER Pathway Category	No pathway information available

6 GO annotations of cellular component

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol	The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
extrinsic component of membrane	The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region.
myofibril	The contractile element of skeletal and cardiac muscle; a long, highly organized bundle of actin, myosin, and other proteins that contracts by a sliding filament mechanism.
plasma membrane	The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
sarcomere	The repeating unit of a myofibril in a muscle cell, composed of an array of overlapping thick and thin filaments between two adjacent Z discs.

1 GO annotations of molecular function

Name	Definition
guanyl-nucleotide exchange factor activity	Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions.

3 GO annotations of biological process

Name	Definition
axon guidance	The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues.
regulation of Rho protein signal transduction	Any process that modulates the frequency, rate or extent of Rho protein signal transduction.
regulation of small GTPase mediated signal transduction	Any process that modulates the frequency, rate or extent of small GTPase mediated signal transduction.

7 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q86YR7	MCF2L2	Probable guanine nucleotide exchange factor MCF2L2	Homo sapiens (Human)	SS
O75962	TRIO	Triple functional domain protein	Homo sapiens (Human)	EV
O60229	KALRN	Kalirin	Homo sapiens (Human)	SS
P10911	MCF2	Proto-oncogene DBL	Homo sapiens (Human)	EV
O15068	MCF2L	Guanine nucleotide exchange factor DBS	Homo sapiens (Human)	SS
Q9CWR0	Arhgef25	Rho guanine nucleotide exchange factor 25	Mus musculus (Mouse)	SS
Q6P720	Arhgef25	Rho guanine nucleotide exchange factor 25	Rattus norvegicus (Rat)	SS

10	20	30	40	50	60
MRGGHKGGRC	ACPRVIRKVL	AKCGCCFARG	GRESYSIAGS	EGSISASAAS	GLAAPSGPSS
70	80	90	100	110	120
GLSSGPCSPG	PPGPVSGLRR	WLDHSKHCLS	VETEADSGQA	GPYENWMLEP	ALATGEELPE
130	140	150	160	170	180
LTLLTTLLEG	PGDKTQPPEE	ETLSQAPESE	EEQKKKALER	SMYVLSELVE	TEKMYVDDLG
190	200	210	220	230	240
QIVEGYMATM	AAQGVPESLR	GRDRIVFGNI	QQIYEWHRDY	FLQELQRCLK	DPDWLAQLFI
250	260	270	280	290	300
KHERRLHMYV	VYCQNKPKSE	HVVSEFGDSY	FEELRQQLGH	RLQLNDLLIK	PVQRIMKYQL
310	320	330	340	350	360
LLKDFLKYYN	RAGMDTADLE	QAVEVMCFVP	KRCNDMMTLG	RLRGFEGKLT	AQGKLLGQDT
370	380	390	400	410	420
FWVTEPEAGG	LLSSRGRERR	VFLFEQIIIF	SEALGGGVRG	GTQPGYVYKN	SIKVSCLGLE
430	440	450	460	470	480
GNLQGDPCRF	ALTSRGPEGG	IQRYVLQAAD	PAISQAWIKH	VAQILESQRD	FLNALQSPIE
490	500	510	520	530	540
YQRRESQTNS	LGRPRGPGVG	SPGRIQLGDQ	AQGSTHTPIN	GSLPSLLLSP	KGEVARALLP
550	560	570
LDKQALGDIP	QAPHDSPPVS	PTPKTPPCQA	RLAKLDEDEL