AiPD: Autoinhibited Protein Database

Q86TA1

Gene name	MOB3B (C9orf35, MOBKL2B)
Protein name	MOB kinase activator 3B
Names	Mob1 homolog 2b , Mps one binder kinase activator-like 2B , MOB kinase activator-like 2B
Species	Homo sapiens (Human)
KEGG Pathway	hsa:79817
EC number
Protein Class

Descriptions

MOB1A is a multifunctional protein involved in key signaling pathways like Hippo and NDR pathway signaling in metazoans and the Mitotic Exit Network in yeast. It regulates protein interactions by binding to upstream kinases MST1 and MST2 and downstream AGC group kinases LATS1, LATS2, NDR1, and NDR2. The autoinhibition of MOB1A occurs when its nonphosphorylated N-terminal extension binds to the core domain, blocking the kinase-binding site. This inhibitory interaction is relieved upon phosphorylation at specific sites (T12 and T35), which induces a conformational change that exposes the kinase-binding surface. Phosphorylation of MOB1A at T12 and T35 is crucial for regulating its interactions with both upstream and downstream kinases. The phosphorylation status dictates the binding affinity of MOB1A, enabling or inhibiting interactions with other proteins. The phosphorylation-dependent conformational changes in MOB1A are essential for the proper functioning of the Hippo signaling pathway, which is critical for cell proliferation and tissue growth regulation.

Autoinhibitory domains (AIDs)

26-41 (N-terminal extension) SS

Target domain	55-216 (MOB1A core domain)
Relief mechanism	PTM
Assay

AID

26-41 (N-terminal extension)

Target domain

55-216 (MOB1A core domain)

Relief mechanism

PTM (Phosphorylation of Thr12 and Thr35)

Assay

Accessory elements

No accessory elements

References

Xiong S et al. (2017) "Regulation of Protein Interactions by Mps One Binder (MOB1) Phosphorylation", Molecular & cellular proteomics : MCP, 16, 1111-1125

Autoinhibited structure

Activated structure

0 structures for Q86TA1

Entry ID	Method	Resolution	Chain	Position	Source
No available structures

208 variants for Q86TA1

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
COSM3656766	2	S>F	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs767376313	3	I>M		No	ExAC TOPMed gnomAD
rs750225512	3	I>V		No	ExAC TOPMed gnomAD
rs898672213	10	N>S		No	TOPMed
rs1822856230	11	K>E		No	TOPMed
rs1037651757	12	D>N		No	TOPMed gnomAD
rs1176354876	15	F>L		No	TOPMed
rs991096104	16	R>*		No	TOPMed gnomAD
rs138812584	16	R>Q		No	ESP TOPMed gnomAD
rs1361591859	19	R>K		No	TOPMed
TCGA novel	19	R>M	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs149640116	20	K>R		No	1000Genomes ESP ExAC TOPMed gnomAD
rs377129841	21	F>L		No	ESP TOPMed gnomAD
rs1376217122	21	F>S		No	gnomAD
TCGA novel	22	E>*	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA
rs1221388542	23	P>R		No	TOPMed gnomAD
rs763076499	23	P>S		No	ExAC TOPMed gnomAD
rs1277912071	26	Q>*		No	TOPMed
rs746279677	26	Q>H		No	ExAC gnomAD
rs781559561	27	R>G		No	ExAC gnomAD
rs771242832	27	R>K		No	ExAC gnomAD
rs747518330	27	R>S		No	ExAC TOPMed gnomAD
rs778281749	29	E>D		No	ExAC gnomAD
rs1822855374	31	H>R		No	TOPMed gnomAD
rs773515192	33	R>G		No	ExAC TOPMed gnomAD
rs779608912	33	R>Q		No	ExAC TOPMed gnomAD
rs773515192	33	R>W		No	ExAC TOPMed gnomAD
rs755857148	34	A>T		No	ExAC gnomAD
rs1822855210	35	Q>E		No	Ensembl
rs767427574	40	S>L		No	ExAC TOPMed gnomAD
TCGA novel	40	S>P	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1270521192	41	G>C		No	TOPMed
rs1822854873	41	G>D		No	Ensembl
COSM1701016 rs1270521192	41	G>S	skin [Cosmic]	No	cosmic curated TOPMed
rs764088305	43	D>E		No	ExAC gnomAD
rs1822854849	43	D>N		No	Ensembl
rs762983513	45	K>Q		No	ExAC TOPMed gnomAD
rs1190980245	46	A>V		No	TOPMed
TCGA novel	47	A>S	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs759783096	49	Q>*		No	ExAC gnomAD
rs771295786	49	Q>H		No	ExAC TOPMed gnomAD
rs1353710156	50	L>S		No	TOPMed gnomAD
rs1822854452	51	P>S		No	TOPMed
rs1360003062	55	D>E		No	TOPMed
rs1822854332	57	N>T		No	TOPMed
rs1822854287	59	W>*		No	gnomAD
rs1822854287	59	W>C		No	gnomAD
rs1425310332	61	A>S		No	TOPMed gnomAD
rs1425310332	61	A>T		No	TOPMed gnomAD
rs1416475746	63	H>P		No	gnomAD
rs1359463807	63	H>Y		No	TOPMed gnomAD
rs1822854083	64	V>A		No	TOPMed gnomAD
rs1822854083	64	V>G		No	TOPMed gnomAD
rs773655912	64	V>L		No	ExAC gnomAD
COSM3906742	65	V>A	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1402864867	66	D>E		No	gnomAD
rs1822854030	66	D>Y		No	gnomAD
rs1343869111	67	F>L		No	Ensembl
rs376544672	69	N>I		No	ESP ExAC TOPMed gnomAD
rs376544672	69	N>S		No	ESP ExAC TOPMed gnomAD
rs1021917104 COSM1461901	70	R>Q	large_intestine [Cosmic]	No	cosmic curated gnomAD
COSM28369 rs748559122	70	R>W	Variant assessed as Somatic; MODERATE impact. pleura [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated ExAC NCI-TCGA TOPMed gnomAD
TCGA novel	71	I>T	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs779649858	72	N>K		No	ExAC gnomAD
TCGA novel	73	L>H	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs755621400	74	I>F		No	ExAC
rs745531879	75	Y>C		No	ExAC TOPMed gnomAD
rs1822853586	76	G>D		No	TOPMed
COSM1108014	77	T>I	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs138589657	78	I>T		No	1000Genomes ExAC gnomAD
rs1245649385	79	C>F		No	gnomAD
TCGA novel rs1822853419	81	F>L	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	TOPMed gnomAD NCI-TCGA
COSM753693	83	T>I	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1489512365	83	T>N		No	gnomAD
rs1187401903	83	T>P		No	TOPMed gnomAD
rs1187401903	83	T>S		No	TOPMed gnomAD
rs202151673	84	E>K		No	ExAC TOPMed gnomAD
rs1822853189	84	E>V		No	TOPMed
rs79546166	85	R>L		No	1000Genomes ESP ExAC TOPMed gnomAD
rs79546166	85	R>Q		No	1000Genomes ESP ExAC TOPMed gnomAD
rs752536593	85	R>W		No	ExAC TOPMed gnomAD
rs1305337873	87	C>R		No	gnomAD
rs1822852884	89	V>A		No	Ensembl
rs1822852924	89	V>M		No	TOPMed gnomAD
rs137917626	91	S>*		No	ESP ExAC gnomAD
rs137917626	91	S>L		No	ESP ExAC gnomAD
TCGA novel	93	G>A	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA
rs1214929618	93	G>S		No	TOPMed gnomAD
rs773601227	94	P>H		No	ExAC TOPMed gnomAD
rs773601227	94	P>L		No	ExAC TOPMed gnomAD
rs1822852697	94	P>S		No	gnomAD
rs996773968	95	K>R		No	TOPMed gnomAD
rs996773968	95	K>T		No	TOPMed gnomAD
rs748695460	96	Y>F		No	ExAC gnomAD
rs769487918	97	E>*		No	ExAC gnomAD
rs745368772	98	Y>D		No	ExAC gnomAD
TCGA novel rs756949269	99	R>L	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA ExAC TOPMed gnomAD
rs756949269	99	R>P		No	ExAC TOPMed gnomAD
rs756949269	99	R>Q	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	ExAC NCI-TCGA TOPMed gnomAD
COSM5106265 rs781031539	99	R>W	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic ExAC NCI-TCGA TOPMed gnomAD
rs746592960	100	W>*		No	ExAC gnomAD
rs746592960	100	W>C		No	ExAC gnomAD
rs758059560	105	K>T		No	ExAC gnomAD
rs1414945874	106	Y>H		No	gnomAD
rs901998161	108	K>Q		No	Ensembl
rs778805959	109	P>A		No	ExAC TOPMed gnomAD
rs1380150875	109	P>L		No	TOPMed
rs1479406758	110	T>A		No	TOPMed gnomAD
rs1250392384	110	T>I		No	TOPMed gnomAD
rs1250392384	110	T>R		No	TOPMed gnomAD
rs755115358	111	A>V		No	ExAC TOPMed gnomAD
rs766633815	113	P>S		No	ExAC gnomAD
rs1329745419	115	P>A		No	TOPMed gnomAD
rs750549062	115	P>H		No	ExAC TOPMed gnomAD
rs750549062	115	P>L		No	ExAC TOPMed gnomAD
rs1004991189	116	Q>H		No	TOPMed gnomAD
rs779954586	117	Y>D		No	Ensembl
rs767941389	118	M>V		No	ExAC TOPMed gnomAD
rs1822851454	119	N>S		No	TOPMed
rs762073422	120	L>F	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	ExAC NCI-TCGA gnomAD
TCGA novel	121	L>F	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1822851391	121	L>P		No	Ensembl
TCGA novel	124	W>*	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA
rs1185116481	125	I>T		No	TOPMed gnomAD
TCGA novel	126	E>G	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA
rs1822851283	130	N>D		No	TOPMed
rs369715922	130	N>K		No	ESP ExAC TOPMed gnomAD
rs775066044	130	N>S		No	ExAC gnomAD
rs759167100	131	N>S		No	ExAC TOPMed gnomAD
rs747633301 COSM1193121	132	E>K	lung [Cosmic]	No	cosmic curated ExAC TOPMed gnomAD
COSM3656765	133	E>D	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1379870171	133	E>G		No	gnomAD
rs746677952	134	I>L		No	ExAC gnomAD
rs1487075687	136	P>L		No	gnomAD
rs955968797	136	P>S		No	TOPMed
rs1416760834	137	T>P		No	gnomAD
rs930653947	138	C>S		No	gnomAD
rs1458774765	139	V>L		No	TOPMed gnomAD
rs1458774765	139	V>M		No	TOPMed gnomAD
rs1335732312	140	G>D		No	TOPMed gnomAD
COSM5173154	140	G>R	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs199832959	143	F>L		No	1000Genomes ExAC gnomAD
rs1821237278	144	P>L		No	Ensembl
rs1277273918	150	I>M		No	gnomAD
rs149013067	151	C>F		No	ESP ExAC TOPMed gnomAD
rs149013067	151	C>Y		No	ESP ExAC TOPMed gnomAD
COSM6183393	152	K>*	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs17853140	152	K>M		No	TOPMed
rs1821236491	152	K>N		No	TOPMed
rs17853140	152	K>T		No	TOPMed
rs1821236395	153	K>*		No	TOPMed
rs1009665462	154	I>F		No	Ensembl
rs2131356297	155	L>M		No	Ensembl
rs1281367641	157	R>C		No	TOPMed gnomAD
COSM4702806 rs201461180	157	R>H	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic 1000Genomes ExAC NCI-TCGA TOPMed gnomAD
rs1295392659	160	R>Q		No	gnomAD
COSM3367683 rs769593320	160	R>W	kidney [Cosmic]	No	cosmic curated ExAC gnomAD
rs745800217	161	V>A		No	ExAC gnomAD
rs956404831	161	V>I		No	TOPMed gnomAD
rs1821235815	162	F>S		No	TOPMed
COSM1461896	163	V>A	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1821235768	163	V>L		No	Ensembl
COSM3656764	164	H>Y	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1483766135	165	V>A		No	TOPMed
rs376064786	165	V>F		No	ESP ExAC TOPMed gnomAD
rs376064786	165	V>I		No	ESP ExAC TOPMed gnomAD
rs1821235390	169	H>P		No	gnomAD
TCGA novel rs1821235390	169	H>R	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA gnomAD
rs748349617	170	F>L		No	ExAC TOPMed gnomAD
rs778180076	170	F>S		No	ExAC gnomAD
COSM1701015 rs1192445543	171	D>N	skin [Cosmic]	No	cosmic curated gnomAD
rs1192445543	171	D>Y		No	gnomAD
rs779174669	172	R>G		No	ExAC TOPMed gnomAD
rs899665493	172	R>L		No	TOPMed
rs899665493	172	R>Q		No	TOPMed
COSM1108013 rs779174669	172	R>W	Variant assessed as Somatic; MODERATE impact. endometrium [NCI-TCGA, Cosmic]	No	NCI-TCGA Cosmic cosmic curated ExAC NCI-TCGA TOPMed gnomAD
rs372110318	174	I>T		No	ESP TOPMed gnomAD
rs200555457	174	I>V		No	Ensembl
rs79647103	175	V>E		No	Ensembl
rs1451321489	175	V>M		No	TOPMed gnomAD
rs982864456	176	M>T		No	TOPMed gnomAD
rs1232389629	177	G>D		No	TOPMed gnomAD
rs1312722921	178	A>T		No	gnomAD
rs1008135553	181	H>Q		No	Ensembl
rs1376430694	182	V>G		No	gnomAD
rs1332362998	183	N>H		No	gnomAD
rs1821234460	186	Y>C		No	Ensembl
rs1821234428	188	H>P		No	Ensembl
rs763546392	189	F>V		No	ExAC gnomAD
rs150259561	191	Y>F		No	1000Genomes ESP ExAC TOPMed gnomAD
rs1177599277	191	Y>H		No	gnomAD
rs981366339	192	F>L		No	TOPMed gnomAD
rs981366339	192	F>V		No	TOPMed gnomAD
rs1821233750	194	T>R		No	TOPMed
TCGA novel	195	E>*	Variant assessed as Somatic; HIGH impact. [NCI-TCGA]	No	NCI-TCGA
rs1345546013	196	M>K		No	gnomAD
rs1821233689	200	D>H		No	gnomAD
rs141372110	201	R>C		No	ESP ExAC TOPMed gnomAD
COSM245741 rs141477702	201	R>H	prostate [Cosmic]	No	cosmic curated 1000Genomes ExAC TOPMed gnomAD
rs141477702	201	R>L		No	1000Genomes ExAC TOPMed gnomAD
rs747424920	210	M>T		No	ExAC TOPMed gnomAD
COSM4913082	211	T>A	Variant assessed as Somatic; MODERATE impact. [NCI-TCGA]	No	NCI-TCGA Cosmic
rs1319091705	211	T>M		No	TOPMed gnomAD
rs146607219	212	S>I		No	ESP ExAC TOPMed
rs779837127	214	M>I		No	ExAC TOPMed gnomAD
rs1587135102	214	M>L		No	Ensembl
rs756016267	216	H>Q		No	ExAC gnomAD
rs1563841780	216	H>Y		No	Ensembl

No associated diseases with Q86TA1

No regional properties for Q86TA1

Type	Name	Position	InterPro Accession
No domain, repeats, and functional sites for Q86TA1

Functions

		Description
EC Number
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

2 GO annotations of cellular component

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

2 GO annotations of molecular function

Name	Definition
metal ion binding	Binding to a metal ion.
protein kinase activator activity	Binds to and increases the activity of a protein kinase, an enzyme which phosphorylates a protein.

3 GO annotations of biological process

Name	Definition
positive regulation of protein phosphorylation	Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein.
regulation of hippo signaling	Any process that modulates the frequency, rate or extent of hippo signaling.
signal transduction	The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.

11 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
P43563	MOB2	CBK1 kinase activator protein MOB2	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	PR
P40484	MOB1	DBF2 kinase activator protein MOB1	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	SS
Q70IA8	MOB3C	MOB kinase activator 3C	Homo sapiens (Human)	SS
Q96BX8	MOB3A	MOB kinase activator 3A	Homo sapiens (Human)	SS
Q9H8S9	MOB1A	MOB kinase activator 1A	Homo sapiens (Human)	EV
Q7L9L4	MOB1B	MOB kinase activator 1B	Homo sapiens (Human)	SS
Q8BSU7	Mob3a	MOB kinase activator 3A	Mus musculus (Mouse)	SS
Q8BJG4	Mob3c	MOB kinase activator 3C	Mus musculus (Mouse)	SS
Q8VE04	Mob3b	MOB kinase activator 3B	Mus musculus (Mouse)	SS
Q8GYX0	MOB1B	MOB kinase activator-like 1B	Arabidopsis thaliana (Mouse-ear cress)	SS
Q9FHI1	MOB1A	MOB kinase activator-like 1A	Arabidopsis thaliana (Mouse-ear cress)	SS

10	20	30	40	50	60
MSIALKQVFN	KDKTFRPKRK	FEPGTQRFEL	HKRAQASLNS	GVDLKAAVQL	PSGEDQNDWV
70	80	90	100	110	120
AVHVVDFFNR	INLIYGTICE	FCTERTCPVM	SGGPKYEYRW	QDDLKYKKPT	ALPAPQYMNL
130	140	150	160	170	180
LMDWIEVQIN	NEEIFPTCVG	VPFPKNFLQI	CKKILCRLFR	VFVHVYIHHF	DRVIVMGAEA
190	200	210
HVNTCYKHFY	YFVTEMNLID	RKELEPLKEM	TSRMCH