AiPD: Autoinhibited Protein Database

Q852Q1

Gene name	OSK4 (OSK24, SNRK1B, LOC_Os08g37800, Os08g0484600, OsJ_27714, OSNPB_080484600, P0419H09.18)
Protein name	Serine/threonine protein kinase OSK4
Names	OsK4 , EC 2.7.11.1 , SUCROSE NON-FERMENTING-1 related protein kinase 1B , SNF1-related kinase 1B , SnRK1B , Serine/threonine protein kinase OSK24 , OsK24
Species	Oryza sativa subsp. japonica (Rice)
KEGG Pathway	osa:4345873
EC number	2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

AMP-activated protein kinase (AMPK) acts as an energy sensor, being activated by metabolic stresses and regulating cellular metabolism. AMPK is a heterotrimer consisting of a catalytic alpha subunit and two regulatory subunits, beta and gamma. The human AMPK alpha2 kinase domain adopts the autoinhibited structure of the DFG-out conformation. The autoinhibitory domain following the kinase domain contributes to the stabilization of the DFG-out autoinhibitory structure. AMPK alpha2 activation is mediated by the phosphorylation of Thr located in the activation loop. The phosphorylated and/or unphosphorylated Thr interacts with residues in the regulatory subunits which stabilize the kinase-domain conformation in either the active or inactive mode.

Autoinhibitory domains (AIDs)

292-334 (autoinhibitory region) SS

Target domain	17-269 (Protein kinase domain)
Relief mechanism	PTM, Ligand binding
Assay

AID

292-334 (autoinhibitory region)

Target domain

17-269 (Protein kinase domain)

Relief mechanism

PTM (Phosphorylation at Thr-172 within the activation loop), Ligand binding (AMP binding)

Assay

Accessory elements

157-179 (Activation loop from InterPro)

Target domain	17-269 (Protein kinase domain)
Relief mechanism
Assay

References

Pang T et al. (2007) "Conserved alpha-helix acts as autoinhibitory sequence in AMP-activated protein kinase alpha subunits", The Journal of biological chemistry, 282, 495-506

Hardie DG et al. (2012) "AMP-activated protein kinase: a target for drugs both ancient and modern", Chemistry & biology, 19, 1222-36

Handa N et al. (2011) "Structural basis for compound C inhibition of the human AMP-activated protein kinase α2 subunit kinase domain", Acta crystallographica. Section D, Biological crystallography, 67, 480-7

Littler DR et al. (2010) "A conserved mechanism of autoinhibition for the AMPK kinase domain: ATP-binding site and catalytic loop refolding as a means of regulation", Acta crystallographica. Section F, Structural biology and crystallization communications, 66, 143-51

Autoinhibited structure

Activated structure

1 structures for Q852Q1

Entry ID	Method	Resolution	Chain	Position	Source
AF-Q852Q1-F1	Predicted				AlphaFoldDB

No variants for Q852Q1

Variant ID(s)	Position	Change	Description	Diseaes Association	Provenance
No variants for Q852Q1

No associated diseases with Q852Q1

1 regional properties for Q852Q1

Type	Name	Position	InterPro Accession
domain	Small GTP-binding protein domain	16 - 141	IPR005225

Functions

		Description
EC Number	2.7.11.1	Protein-serine/threonine kinases
Subcellular Localization	Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category	No pathway information available

2 GO annotations of cellular component

Name	Definition
cytoplasm	The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus	A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name	Definition
ATP binding	Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
protein serine kinase activity	Catalysis of the reactions
protein serine/threonine kinase activity	Catalysis of the reactions

2 GO annotations of biological process

Name	Definition
intracellular signal transduction	The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
phosphorylation	The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

85 homologous proteins in AiPD

UniProt AC	Gene Name	Protein Name	Species	Evidence Code
Q02066		Abscisic acid-inducible protein kinase	Triticum aestivum (Wheat)	PR
P06782	SNF1	Carbon catabolite-derepressing protein kinase	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	EV
Q5GLH2	TRIB2	Tribbles homolog 2	Bos taurus (Bovine)	SS
Q0VCE3	TRIB3	Tribbles homolog 3	Bos taurus (Bovine)	SS
Q3SZW1	TSSK1B	Testis-specific serine/threonine-protein kinase 1	Bos taurus (Bovine)	PR
Q92519	TRIB2	Tribbles homolog 2	Homo sapiens (Human)	SS
Q96RU7	TRIB3	Tribbles homolog 3	Homo sapiens (Human)	PR
Q14680	MELK	Maternal embryonic leucine zipper kinase	Homo sapiens (Human)	EV
Q96RU8	TRIB1	Tribbles homolog 1	Homo sapiens (Human)	EV
Q15831	STK11	Serine/threonine-protein kinase STK11	Homo sapiens (Human)	PR
Q96RG2	PASK	PAS domain-containing serine/threonine-protein kinase	Homo sapiens (Human)	EV
Q9BXA7	TSSK1B	Testis-specific serine/threonine-protein kinase 1	Homo sapiens (Human)	PR
P54646	PRKAA2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Homo sapiens (Human)	EV
Q7KZI7	MARK2	Serine/threonine-protein kinase MARK2	Homo sapiens (Human)	SS
Q9P0L2	MARK1	Serine/threonine-protein kinase MARK1	Homo sapiens (Human)	EV
P27448	MARK3	MAP/microtubule affinity-regulating kinase 3	Homo sapiens (Human)	SS
Q96L34	MARK4	MAP/microtubule affinity-regulating kinase 4	Homo sapiens (Human)	SS
Q9NRH2	SNRK	SNF-related serine/threonine-protein kinase	Homo sapiens (Human)	SS
P57059	SIK1	Serine/threonine-protein kinase SIK1	Homo sapiens (Human)	PR
O60285	NUAK1	NUAK family SNF1-like kinase 1	Homo sapiens (Human)	PR
Q13131	PRKAA1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Homo sapiens (Human)	EV
Q8K4K3	Trib2	Tribbles homolog 2	Mus musculus (Mouse)	SS
Q61241	Tssk1b	Testis-specific serine/threonine-protein kinase 1	Mus musculus (Mouse)	PR
Q8K4K2	Trib3	Tribbles homolog 3	Mus musculus (Mouse)	SS
Q61846	Melk	Maternal embryonic leucine zipper kinase	Mus musculus (Mouse)	PR
O54863	Tssk2	Testis-specific serine/threonine-protein kinase 2	Mus musculus (Mouse)	PR
Q8K4K4	Trib1	Tribbles homolog 1	Mus musculus (Mouse)	SS
Q5EG47	Prkaa1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Mus musculus (Mouse)	SS
Q8BRK8	Prkaa2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Mus musculus (Mouse)	SS
Q05512	Mark2	Serine/threonine-protein kinase MARK2	Mus musculus (Mouse)	SS
Q8VHJ5	Mark1	Serine/threonine-protein kinase MARK1	Mus musculus (Mouse)	SS
Q03141	Mark3	MAP/microtubule affinity-regulating kinase 3	Mus musculus (Mouse)	SS
Q8CIP4	Mark4	MAP/microtubule affinity-regulating kinase 4	Mus musculus (Mouse)	SS
Q8C0X8		Sperm motility kinase X	Mus musculus (Mouse)	PR
A0AUV4	Gm7168	Sperm motility kinase Y	Mus musculus (Mouse)	PR
Q8C0N0	Gm4922	Sperm motility kinase Z	Mus musculus (Mouse)	PR
Q641K5	Nuak1	NUAK family SNF1-like kinase 1	Mus musculus (Mouse)	PR
A2KF29	Smoktcr	Sperm motility kinase Tcr mutant form	Mus musculus (Mouse)	PR
Q28948	PRKAA2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Sus scrofa (Pig)	SS
Q09136	PRKAA1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Sus scrofa (Pig)	SS
Q9WTQ6	Trib3	Tribbles homolog 3	Rattus norvegicus (Rat)	SS
P54645	Prkaa1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Rattus norvegicus (Rat)	EV
O08679	Mark2	Serine/threonine-protein kinase MARK2	Rattus norvegicus (Rat)	SS
O08678	Mark1	Serine/threonine-protein kinase MARK1	Rattus norvegicus (Rat)	SS
Q8VHF0	Mark3	MAP/microtubule affinity-regulating kinase 3	Rattus norvegicus (Rat)	SS
Q09137	Prkaa2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Rattus norvegicus (Rat)	EV
Q5QNM6	CIPK13	Putative CBL-interacting protein kinase 13	Oryza sativa subsp japonica (Rice)	PR
Q5JLQ9	CIPK30	CBL-interacting protein kinase 30	Oryza sativa subsp japonica (Rice)	PR
Q852Q0	OSK3	Serine/threonine protein kinase OSK3	Oryza sativa subsp. japonica (Rice)	SS
Q6ERS4	CIPK16	CBL-interacting protein kinase 16	Oryza sativa subsp japonica (Rice)	PR
Q0D4J7	SAPK2	Serine/threonine-protein kinase SAPK2	Oryza sativa subsp japonica (Rice)	PR
Q5N942	SAPK4	Serine/threonine-protein kinase SAPK4	Oryza sativa subsp japonica (Rice)	PR
Q75H77	SAPK10	Serine/threonine-protein kinase SAPK10	Oryza sativa subsp japonica (Rice)	PR
Q75LR7	SAPK1	Serine/threonine-protein kinase SAPK1	Oryza sativa subsp japonica (Rice)	PR
Q75V57	SAPK9	Serine/threonine-protein kinase SAPK9	Oryza sativa subsp japonica (Rice)	PR
Q7XQP4	SAPK7	Serine/threonine-protein kinase SAPK7	Oryza sativa subsp japonica (Rice)	PR
Q7Y0B9	SAPK8	Serine/threonine-protein kinase SAPK8	Oryza sativa subsp japonica (Rice)	PR
Q852Q2	OSK1	Serine/threonine protein kinase OSK1	Oryza sativa subsp. japonica (Rice)	SS
Q8LIG4	CIPK3	CBL-interacting protein kinase 3	Oryza sativa subsp japonica (Rice)	PR
Q6ZLP5	CIPK23	CBL-interacting protein kinase 23	Oryza sativa subsp japonica (Rice)	PR
P0C5D6	SAPK3	Serine/threonine-protein kinase SAPK3	Oryza sativa subsp japonica (Rice)	PR
Q2RAX3	CIPK33	CBL-interacting protein kinase 33	Oryza sativa subsp japonica (Rice)	PR
Q2QY53	CIPK32	CBL-interacting protein kinase 32	Oryza sativa subsp japonica (Rice)	PR
Q21017	kin-29	Serine/threonine-protein kinase kin-29	Caenorhabditis elegans	SS
P45894	aak-1	5'-AMP-activated protein kinase catalytic subunit alpha-1	Caenorhabditis elegans	SS
Q95ZQ4	aak-2	5'-AMP-activated protein kinase catalytic subunit alpha-2	Caenorhabditis elegans	SS
P43291	SRK2A	Serine/threonine-protein kinase SRK2A	Arabidopsis thaliana (Mouse-ear cress)	PR
Q93VD3	CIPK23	CBL-interacting serine/threonine-protein kinase 23	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9C958	SRK2B	Serine/threonine-protein kinase SRK2B	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9M9E9	SRK2C	Serine/threonine-protein kinase SRK2C	Arabidopsis thaliana (Mouse-ear cress)	PR
O64812	SRK2J	Serine/threonine-protein kinase SRK2J	Arabidopsis thaliana (Mouse-ear cress)	PR
Q2V452	CIPK3	CBL-interacting serine/threonine-protein kinase 3	Arabidopsis thaliana (Mouse-ear cress)	SS
O22971	CIPK13	CBL-interacting serine/threonine-protein kinase 13	Arabidopsis thaliana (Mouse-ear cress)	PR
Q39192	SRK2D	Serine/threonine-protein kinase SRK2D	Arabidopsis thaliana (Mouse-ear cress)	PR
O65554	CIPK6	CBL-interacting serine/threonine-protein kinase 6	Arabidopsis thaliana (Mouse-ear cress)	PR
Q940H6	SRK2E	Serine/threonine-protein kinase SRK2E	Arabidopsis thaliana (Mouse-ear cress)	PR
P43292	SRK2G	Serine/threonine-protein kinase SRK2G	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9LDI3	CIPK24	CBL-interacting serine/threonine-protein kinase 24	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FJ54	CIPK20	CBL-interacting serine/threonine-protein kinase 20	Arabidopsis thaliana (Mouse-ear cress)	PR
Q94CG0	CIPK21	CBL-interacting serine/threonine-protein kinase 21	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FFP9	SRK2H	Serine/threonine-protein kinase SRK2H	Arabidopsis thaliana (Mouse-ear cress)	PR
Q39193	SRK2I	Serine/threonine-protein kinase SRK2I	Arabidopsis thaliana (Mouse-ear cress)	PR
Q9FLZ3	KIN12	SNF1-related protein kinase catalytic subunit alpha KIN12	Arabidopsis thaliana (Mouse-ear cress)	SS
P92958	KIN11	SNF1-related protein kinase catalytic subunit alpha KIN11	Arabidopsis thaliana (Mouse-ear cress)	PR
Q38997	KIN10	SNF1-related protein kinase catalytic subunit alpha KIN10	Arabidopsis thaliana (Mouse-ear cress)	SS

10	20	30	40	50	60
MEGNARGGGH	SEALKNYNLG	RTLGIGSFGK	VKIAEHKLTG	HRVAIKILNR	RQMRNMEMEE
70	80	90	100	110	120
KAKREIKILR	LFIHPHIIRL	YEVIYTPTDI	YVVMEYCKFG	ELFDYIVEKG	RLQEDEARRI
130	140	150	160	170	180
FQQIISGVEY	CHRNMVVHRD	LKPENLLLDS	KYNVKLADFG	LSNVMHDGHF	LKTSCGSPNY
190	200	210	220	230	240
AAPEVISGKL	YAGPEVDVWS	CGVILYALLC	GTLPFDDENI	PNLFKKIKGG	IYTLPSHLSA
250	260	270	280	290	300
LARDLIPRML	VVDPMKRITI	REIREHQWFQ	IRLPRYLAVP	PPDTAQQAKM	IDEDTLQDVV
310	320	330	340	350	360
NLGYEKDHVC	ESLRNRLQNE	ATVAYYLLLD	NRFRATSGYL	GADYQESLER	NLNRFASSES
370	380	390	400	410	420
ASSNTRHYLP	GSSDPHASGL	RPHYPVERKW	ALGLQSRAQP	REIMIEVLKA	LEDLNVCWKK
430	440	450	460	470	480
NGQYNMKCRW	SVGYPQATDM	LDVNHSFVDD	SIIMDNGDVN	GRLPAVIKFE	IQLYKSRDEK
490	500
YLLDMQRVTG	PQLLFLDFCA	AFLTKLRVL