Q84VX4

SS
Gene name
MPS1 (PPK1, At1g77720, T32E8.5)
Protein name
Serine/threonine-protein kinase MPS1
Names
EC 2.7.12.2 , Monopolar spindle protein 1 homolog
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT1G77720
EC number
2.7.12.2: Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Protein Class

Descriptions

Monopolar spindle 1 (MPS1 or TTK) is a conserved apical kinase in the spindle assembly checkpoint (SAC) that ensures accurate segregation of chromosomes during mitosis. MPS1 undergoes extensive auto- and transphosphorylation. The N-terminal extension harbors multiple phosphorylation sites and is involved in dimerization and catalytic activation. Phosphorylation within the N-terminal extension contributes to MPS1 activation through relief of catalytic autoinhibition that is mediated by the N-terminal extension itself.

Autoinhibitory domains (AIDs)

22-31 (N-terminal extension) SS

Target domain

400-693 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

567-592 (Activation loop from InterPro)

Target domain

400-693 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q84VX4

Entry ID Method Resolution Chain Position Source
AF-Q84VX4-F1 Predicted AlphaFoldDB

58 variants for Q84VX4

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH05162937 2 D>N No 1000Genomes
ENSVATH14488774 15 P>T No 1000Genomes
tmp_1_29210796_T_G 23 L>V No 1000Genomes
ENSVATH13828170 68 S>T No 1000Genomes
tmp_1_29211277_G_T 75 A>S No 1000Genomes
tmp_1_29211284_G_A 77 R>K No 1000Genomes
ENSVATH05162948 86 G>R No 1000Genomes
ENSVATH05162948 86 G>W No 1000Genomes
tmp_1_29211368_C_T 105 T>I No 1000Genomes
ENSVATH00146568 116 S>F No 1000Genomes
tmp_1_29211419_C_T 122 A>V No 1000Genomes
ENSVATH05162949 128 T>A No 1000Genomes
tmp_1_29211486_A_C 144 K>N No 1000Genomes
ENSVATH13828171 149 S>N No 1000Genomes
ENSVATH01550455 159 K>T No 1000Genomes
tmp_1_29211551_C_A 166 T>K No 1000Genomes
tmp_1_29211709_G_C 172 W>C No 1000Genomes
ENSVATH05162956 174 A>P No 1000Genomes
tmp_1_29211734_T_A 181 S>T No 1000Genomes
ENSVATH05162957 183 L>I No 1000Genomes
ENSVATH00146569 198 D>N No 1000Genomes
tmp_1_29211795_T_G 201 V>G No 1000Genomes
tmp_1_29211830_C_G 213 L>V No 1000Genomes
tmp_1_29211843_A_T 217 Q>L No 1000Genomes
tmp_1_29211864_C_G 224 P>R No 1000Genomes
tmp_1_29211945_C_T 251 S>L No 1000Genomes
ENSVATH05162958 267 E>K No 1000Genomes
tmp_1_29212005_A_G 271 N>S No 1000Genomes
tmp_1_29212053_G_A 287 C>Y No 1000Genomes
ENSVATH05162959 302 A>D No 1000Genomes
ENSVATH01550458 316 V>L No 1000Genomes
ENSVATH13828174 322 D>Y No 1000Genomes
ENSVATH00146570 323 T>M No 1000Genomes
tmp_1_29212184_A_T 331 T>S No 1000Genomes
tmp_1_29212233_A_G 347 Q>R No 1000Genomes
tmp_1_29212326_G_C 378 G>A No 1000Genomes
ENSVATH05162962 423 T>I No 1000Genomes
ENSVATH13828176 436 D>E No 1000Genomes
ENSVATH05162963 438 A>G No 1000Genomes
tmp_1_29212545_A_G 451 K>R No 1000Genomes
tmp_1_29212548_A_G 452 K>R No 1000Genomes
tmp_1_29212756_A_G 482 S>G No 1000Genomes
tmp_1_29212779_G_C 489 K>N No 1000Genomes
tmp_1_29212884_T_G 524 D>E No 1000Genomes
tmp_1_29213355_C_A 604 S>R No 1000Genomes
tmp_1_29213457_T_G 638 D>E No 1000Genomes
ENSVATH05162972 638 D>N No 1000Genomes
ENSVATH05162973 654 H>N No 1000Genomes
ENSVATH05162974 654 H>P No 1000Genomes
ENSVATH01550462 660 Q>E No 1000Genomes
ENSVATH00146575 700 E>K No 1000Genomes
ENSVATH05162975 739 P>A No 1000Genomes
ENSVATH05162975 739 P>T No 1000Genomes
ENSVATH00146577 740 L>R No 1000Genomes
tmp_1_29213774_A_T 744 D>V No 1000Genomes
ENSVATH05162976 750 D>V No 1000Genomes
tmp_1_29213807_T_G 755 L>R No 1000Genomes
ENSVATH05162977 755 L>V No 1000Genomes

No associated diseases with Q84VX4

4 regional properties for Q84VX4

Type Name Position InterPro Accession
domain Protein kinase domain 400 - 693 IPR000719
active_site Serine/threonine-protein kinase, active site 547 - 559 IPR008271
binding_site Protein kinase, ATP binding site 406 - 428 IPR017441
domain Protein kinase Mps1 family, catalytic domain 398 - 693 IPR027084

Functions

Description
EC Number 2.7.12.2 Dual-specificity kinases (those acting on Ser/Thr and Tyr residues)
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
MAP kinase kinase activity Catalysis of the concomitant phosphorylation of threonine (T) and tyrosine (Y) residues in a Thr-Glu-Tyr (TEY) thiolester sequence in a MAP kinase (MAPK) substrate.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
protein tyrosine kinase activity Catalysis of the reaction

6 GO annotations of biological process

Name Definition
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
meiotic spindle assembly checkpoint signaling A signal transduction process that contributes to a meiotic spindle assembly checkpoint, that delays the metaphase/anaphase transition of a meiotic cell cycle until the spindle is correctly assembled and chromosomes are attached to the spindle.
mitotic spindle assembly checkpoint signaling A mitotic cell cycle checkpoint that delays mitotic sister chromatid separation and consequently the mitotic metaphase/anaphase transition until the spindle is correctly assembled and chromosomes are attached to the spindle. Spindle assembly checkpoint signalling begins with the activated Mph family kinase, and results in the inhibition of the Anaphase Promoting Complex and its activator Sleepy/Cdc20 by the mitotic checkpoint complex (MCC).
organelle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of an organelle within a cell. An organelle is an organized structure of distinctive morphology and function. Includes the nucleus, mitochondria, plastids, vacuoles, vesicles, ribosomes and the cytoskeleton. Excludes the plasma membrane.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein localization to kinetochore Any process in which a protein is transported to, or maintained at, the kinetochore.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P33981 TTK Dual specificity protein kinase TTK Homo sapiens (Human) EV
P35761 Ttk Dual specificity protein kinase TTK Mus musculus (Mouse) SS
Q8AYG3 ttk Dual specificity protein kinase Ttk Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MDREDNLPVQ PDKPPKSLVR PILNLETTSS SSSSSSSSPE LLRHLQAAFK RHRPLSKMQT
70 80 90 100 110 120
TSIGPRRSVA PQRQASRNTR LVTAEGQRSQ DVVTLSQSLA ANTLTQDTTN NLAITSVAGE
130 140 150 160 170 180
SASITQPTVS EHFNPSDRQM DFGKSAVTSL ESNLDVQRKS QSLIGTSQDM EWDATNQAEA
190 200 210 220 230 240
SHLDACIGSK HQNLPSVDSE VSLKSEYKDS SSLAKIQGQL GEFPNFLNQP RTRCSAVGSS
250 260 270 280 290 300
WATTTLIHSS SAPMLNATTH VSRSYVEADS NANPHAVQSQ GNLPSCCPSS KVSNILHPNK
310 320 330 340 350 360
DATASEMPAS TNDPEVRVKE TDTSKQQQIT TGLEAPVGSS IYGSDGQANA RLPEELHTSV
370 380 390 400 410 420
SSQPQKSDKH EKVASSKGPS APRKRNYDPD LFFKVNGKLY QRLGKIGSGG SSEVHKVISS
430 440 450 460 470 480
DCTIYALKKI KLKGRDYATA YGFCQEIGYL KKLKGKTNII QLIDYEVTDK TLLQEVLNGT
490 500 510 520 530 540
MSNKDGRVKE DGFIYMVLEY GEIDLAHMLS QKWREIEGSD RTIDENWLRF YWQQILQAVN
550 560 570 580 590 600
TIHEERIVHS DLKPANFLLV RGFLKLIDFG IAKAINSDTT NIQRDSQVGT LSYMSPEAFM
610 620 630 640 650 660
CNESDENGNT IKCGRPSDIW SLGCILYQMV YGRTPFADYK TFWAKFKVIT DPNHEITYNQ
670 680 690 700 710 720
LSNPWLIDLM KKCLAWDRNQ RWRIPELLQH PFLAPPIPHE PQVKTIKLFS LIAESCGSDD
730 740 750 760 770
DKANSMISQL EQLLSNPAPL PRNDVLDSRD QNQQLLSRVS ELCIQLQDRL QGLEGEN