Q80UE6

SS
Gene name
Wnk4
Protein name
Serine/threonine-protein kinase WNK4
Names
Protein kinase lysine-deficient 4, Protein kinase with no lysine 4
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:69847
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other protein kinases. Rat WNK1 (Q9JIH7) contains an autoinhibitory region C-terminal to the catalytic kinase domain.

Autoinhibitory domains (AIDs)

463-486 (Autoinhibitory region) SS

Target domain

171-429 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

317-338 (Activation loop from InterPro)

Target domain

171-429 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q80UE6

Entry ID Method Resolution Chain Position Source
AF-Q80UE6-F1 Predicted AlphaFoldDB

15 variants for Q80UE6

Variant ID(s) Position Change Description Diseaes Association Provenance
rs217080242 109 M>T No Ensembl
rs213942067 133 S>F No Ensembl
rs250224787 543 L>M No Ensembl
rs232403658 611 E>D No Ensembl
rs235569209 661 V>A No Ensembl
rs27073460 742 T>S No Ensembl
rs256215640 766 A>T No Ensembl
rs27073455 782 I>V No Ensembl
rs234187710 839 S>L No Ensembl
rs254823119 844 Q>R No Ensembl
rs216388261 859 V>A No Ensembl
rs52181149 870 A>S No Ensembl
rs214688602 940 G>D No Ensembl
rs230203846 942 L>R No Ensembl
rs242671939 970 R>C No Ensembl

No associated diseases with Q80UE6

3 regional properties for Q80UE6

Type Name Position InterPro Accession
domain Protein kinase domain 171 - 429 IPR000719
active_site Serine/threonine-protein kinase, active site 295 - 307 IPR008271
domain Serine/threonine-protein kinase OSR1/WNK, CCT domain 450 - 512 IPR024678

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cell junction, tight junction
  • Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct (PubMed:11498583)
  • WNK4 is part of the tight junction complex (PubMed:11498583)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
bicellular tight junction An occluding cell-cell junction that is composed of a branching network of sealing strands that completely encircles the apical end of each cell in an epithelial sheet; the outer leaflets of the two interacting plasma membranes are seen to be tightly apposed where sealing strands are present. Each sealing strand is composed of a long row of transmembrane adhesion proteins embedded in each of the two interacting plasma membranes.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chloride channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a chloride channel.
potassium channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a potassium channel.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

14 GO annotations of biological process

Name Definition
cellular cation homeostasis Any process involved in the maintenance of an internal steady state of cations at the level of a cell.
cellular chloride ion homeostasis Any process involved in the maintenance of an internal steady state of chloride ions at the level of a cell.
chloride transport The directed movement of chloride into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
distal tubule morphogenesis The process in which the anatomical structures of a distal tubule are generated and organized. The distal tubule is a nephron tubule that begins at the macula densa and extends to the connecting tubule.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
ion homeostasis Any process involved in the maintenance of an internal steady state of ions within an organism or cell.
ion transport The directed movement of charged atoms or small charged molecules into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
negative regulation of pancreatic juice secretion Any process that decreases the rate, frequency or extent of pancreatic juice secretion, the regulated release of pancreatic juice by the exocrine pancreas into the upper part of the intestine.
negative regulation of sodium ion transport Any process that decreases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
positive regulation of potassium ion import across plasma membrane Any process that activates or increases the frequency, rate or extent of potassium ion import across the plasma membrane.
positive regulation of sodium ion transmembrane transporter activity Any process that activates or increases the frequency, rate or extent of sodium ion transmembrane transporter activity.
protein localization Any process in which a protein is transported to, or maintained in, a specific location.
protein phosphorylation The process of introducing a phosphate group on to a protein.
renal sodium ion absorption A renal system process in which sodium ions are taken up from the collecting ducts and proximal and distal loops of the nephron. In non-mammalian species, absorption may occur in related structures.

17 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9Y3S1 WNK2 Serine/threonine-protein kinase WNK2 Homo sapiens (Human) SS
Q9H4A3 WNK1 Serine/threonine-protein kinase WNK1 Homo sapiens (Human) SS
Q9BYP7 WNK3 Serine/threonine-protein kinase WNK3 Homo sapiens (Human) SS
Q96J92 WNK4 Serine/threonine-protein kinase WNK4 Homo sapiens (Human) SS
Q99J45 Nrbp1 Nuclear receptor-binding protein Mus musculus (Mouse) PR
Q80XP9 Wnk3 Serine/threonine-protein kinase WNK3 Mus musculus (Mouse) SS
P83741 Wnk1 Serine/threonine-protein kinase WNK1 Mus musculus (Mouse) SS
Q3UH66 Wnk2 Serine/threonine-protein kinase WNK2 Mus musculus (Mouse) SS
Q6R2V0 WNK1 Serine/threonine-protein kinase WNK1 Sus scrofa (Pig) SS
Q9JIH7 Wnk1 Serine/threonine-protein kinase WNK1 Rattus norvegicus (Rat) EV
Q7TPK6 Wnk4 Serine/threonine-protein kinase WNK4 Rattus norvegicus (Rat) SS
X5M5N0 wnk-1 Serine/threonine-protein kinase WNK Caenorhabditis elegans SS
Q8RXE5 WNK10 Probable serine/threonine-protein kinase WNK10 Arabidopsis thaliana (Mouse-ear cress) PR
Q944Q0 WNK8 Serine/threonine-protein kinase WNK8 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LVL5 WNK4 Probable serine/threonine-protein kinase WNK4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STK6 WNK3 Probable serine/threonine-protein kinase WNK3 Arabidopsis thaliana (Mouse-ear cress) PR
Q8LST2 WNK7 Probable serine/threonine-protein kinase WNK7 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MLAPRNTETG VPMSQTEADL ALRPSPALTS TGPTRLGPPP RRVRRFSGKA EPRPRSSRPS
70 80 90 100 110 120
RRSSVDLGLL SSWSQPASLL PEPPDPPDSA GPTRSPPSSS KEPPEGTWMG AAPVKAVDSA
130 140 150 160 170 180
CPELTGSSGG PGSREPPRVP DAAARERRRE QEEKEDTETQ AVATSPDGRY LKFDIEIGRG
190 200 210 220 230 240
SFKTVYRGLD TDTTVEVAWC ELQTRKLSRA ERQRFSEEVE MLKGLQHPNI VRFYDSWKSV
250 260 270 280 290 300
LRGQVCIVLV TELMTSGTLK TYLRRFREMK PRVLQRWSRQ ILRGLHFLHS RVPPILHRDL
310 320 330 340 350 360
KCDNVFITGP SGSVKIGDLG LATLKRASFA KSVIGTPEFM APEMYEEKYD EAVDVYAFGM
370 380 390 400 410 420
CMLEMATSEY PYSECQNAAQ IYRKVTSGTK PNSFYKVKMP EVKEIIEGCI RTDKNERFTI
430 440 450 460 470 480
QDLLAHAFFR EERGVHVELA EEDDGEKPGL KLWLRMEDAR RGGRPRDNQA IEFLFQLGRD
490 500 510 520 530 540
AAEEVAQEMV ALGLVCEADY QPVARAVRER VAAIQRKREK LRKARELEVL PPDSGPPPAT
550 560 570 580 590 600
VSLAPGPPSA FPPEPEEPEA DQHQSFLFRH ASYSSTTSDC ETDGYLSSSG FLDASDPALQ
610 620 630 640 650 660
PPGGLPSSPA ESHLCLPSGF ALSIPRSGPG SDFSPGDSYA SDAASGLSDM GEGGQMRKNP
670 680 690 700 710 720
VKTLRRRPRS RLRVTSVSDQ SDRVVECQLQ THNSKMVTFR FDLDGDSPEE IAAAMVYNEF
730 740 750 760 770 780
ILPSERDGFL SRIREIIQRV ETLLKRDAGP PEAAEDALSP QEEPAALPAL PGPPNAEPQR
790 800 810 820 830 840
SISPEQRSWA AFSTSPSSPG TPLSPGAPFS PGTPPVFPCP IFPITSPSCY PCPFSQVSSN
850 860 870 880 890 900
PYPQAPSSLL PLSSSASQVP LPSSSLPISA PLPFSPSYPQ DPLSPTSLPV CPSPPSLPST
910 920 930 940 950 960
TAAPLLSLAS AFSLAVMTVA QSLLSPSPGL LSQSPPAPPG PLPSLPLSLA SCDQESLSAQ
970 980 990 1000 1010 1020
TAETENEASR NPAQPLLGDA RLAPISEEGK PQLVGRFQVT SSKEPAEPPL QPASPTLSRS
1030 1040 1050 1060 1070 1080
LKLPSPPLTS ESSDTEDSAA GGPETREALA ESDRAAEGLG VAVDDEKDEG KEPLLGGSSP
1090 1100 1110 1120 1130 1140
ILSHPSPVWM NYSYSSLCLS SEESESSGED EEFWAELQNL RQKHLSEVEA LQTLQKKEIE
1150 1160 1170 1180 1190 1200
DLYSRLGKQP PPGIVAPAAM LSCRQRRLSK GSFPTSRRNS LQRSDLPGPG IMRRNSLSGS
1210 1220
STGSQEQRAS KGVTFAGDIG RM