Q80TV8

SS
Gene name
Clasp1 (Kiaa0622)
Protein name
CLIP-associating protein 1
Names
Cytoplasmic linker-associated protein 1
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:76707
EC number
Protein Class

Descriptions

CLASPs, major microtubule-stabilizing factors in interphase and mitosis, prevent microtubule from switching form growth to shortening by stabilizing growing microtubule ends, and thus suppress microtubule catastrophes. CLASP2a consists of three TOG-like domains (termed TOG1, 2, and 3) and a C-terminal domain, CLIP-interacting domain (CLIP-ID) responsible for interaction with CLIP-170 and other partners. CLASP2a TOG2 is necessary and sufficient for catastrophe inhibition. The inhibition function is suppressed by the C-terminal domain CLIP-ID while the TOG1 domain or the CLIP-ID partner binding to CLIP-ID can release the autoinhibition.

Autoinhibitory domains (AIDs)

1418-1527 (C-terminal domain CLIP-ID) SS

Target domain

846-1084 (TOG2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q80TV8

Entry ID Method Resolution Chain Position Source
AF-Q80TV8-F1 Predicted AlphaFoldDB

No variants for Q80TV8

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q80TV8

No associated diseases with Q80TV8

5 regional properties for Q80TV8

Type Name Position InterPro Accession
domain 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain 494 - 818 IPR002073
domain GAF domain 122 - 282 IPR003018-1
domain GAF domain 304 - 471 IPR003018-2
domain HD/PDEase domain 568 - 745 IPR003607
conserved_site 3'5'-cyclic nucleotide phosphodiesterase, conserved site 611 - 622 IPR023174

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Chromosome, centromere, kinetochore
  • Cytoplasm, cytoskeleton, spindle
  • Golgi apparatus, trans-Golgi network
  • Localizes to microtubule plus ends
  • Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase
  • Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase
  • In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella
  • Localizes to the cell cortex and this requires ERC1 and PHLDB2
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

10 GO annotations of cellular component

Name Definition
basal cortex The region that lies just beneath the plasma membrane on the basal edge of a cell.
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasmic microtubule Any microtubule in the cytoplasm of a cell.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
mitotic spindle A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.

2 GO annotations of molecular function

Name Definition
kinetochore binding Binding to a kinetochore, a proteinaceous structure on a condensed chromosome, beside the centromere, to which the spindle fibers are attached.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.

10 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
establishment of cell polarity The specification and formation of anisotropic intracellular organization or cell growth patterns.
establishment of mitotic spindle localization The cell cycle process in which the directed movement of the mitotic spindle to a specific location in the cell occurs.
establishment of spindle orientation Any process that set the alignment of spindle relative to other cellular structures.
microtubule anchoring Any process in which a microtubule is maintained in a specific location in a cell.
microtubule nucleation The process in which tubulin alpha-beta heterodimers begin aggregation to form an oligomeric tubulin structure (a microtubule seed). Microtubule nucleation is the initiating step in the formation of a microtubule in the absence of any existing microtubules ('de novo' microtubule formation).
microtubule organizing center organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a microtubule organizing center, a structure from which microtubules grow.
mitotic spindle assembly Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied.
negative regulation of microtubule depolymerization Any process that stops, prevents, or reduces the frequency, rate or extent of microtubule depolymerization; prevention of depolymerization of a microtubule can result from binding by 'capping' at the plus end (e.g. by interaction with another cellular protein of structure) or by exposing microtubules to a stabilizing drug such as taxol.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38198 STU1 Protein STU1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9NBD7 chb CLIP-associating protein Drosophila melanogaster (Fruit fly) SS
O75122 CLASP2 CLIP-associating protein 2 Homo sapiens (Human) EV
Q7Z460 CLASP1 CLIP-associating protein 1 Homo sapiens (Human) SS
Q8BRT1 Clasp2 CLIP-associating protein 2 Mus musculus (Mouse) SS
Q99JD4 Clasp2 CLIP-associating protein 2 Rattus norvegicus (Rat) SS
Q6NYW6 clasp2 CLIP-associating protein 2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MEPRMESCLA QVLQKDVGKR LQVGQELIDY FSDRQKSADL EHDQTLLDKL VDGLATSWVN
70 80 90 100 110 120
SSNYKVVLLG MDILSALVTR LQDRFKAQIG TVLPSLIDRL GDAKDSVREQ DQTLLLKIMD
130 140 150 160 170 180
QAANPQYVWD RMLGGFKHKN FRTREGICLC LIATLNASGA QTLTLSKIVP HICNLLGDPN
190 200 210 220 230 240
SQVRDAAINS LVEIYRHVGE RVRADLSKKG LPQSRLNVIF TKFDEVQKSG NMIQSANEKN
250 260 270 280 290 300
FDDEDSVDGN RPSSASSSSS KAPSSSRRNV NLGTTRRLMS SSLGSKSSAA KEGAGAVDEE
310 320 330 340 350 360
DFIKAFDDVP VVQIYSSRDL EESINKIREI LSDDKHDWEQ RVNALKKIRS LLLAGAAEYD
370 380 390 400 410 420
NFFQHLRLLD GAFKLSAKDL RSQVVREACI TLGHLSSVLG NKFDHGAEAI MPTIFNLIPN
430 440 450 460 470 480
SAKIMATSGV VAVRLIIRHT HIPRLIPVIT SNCTSKSVAV RRRCFEFLDL LLQEWQTHSL
490 500 510 520 530 540
ERHISVLAET IKKGIHDADS EARIEARKCY WGFHSHFSRE AEHLYHTLES SYQKALQSHL
550 560 570 580 590 600
KNSDSIVSLP QSDRSSSSSQ ESLNRPLSAK RSPTGSTASR GSTVSTKSVS TTGSLQRSRS
610 620 630 640 650 660
DIDVNAAASA KSKVSSSSGS PAFSSAAALP PGSYASLGRI RTRRQSSGST TNVASTPDSR
670 680 690 700 710 720
GRSRAKVVSQ SQRSRSANPA GAGSRSSSPG KLLGSGLAGG SSRGPPVTPS SEKRSKIPRS
730 740 750 760 770 780
QGCSRETSPN RIGLARSSRI PRPSMSQGCS RDTSRESSRD TSPARGFTPL DRFGLGQSGR
790 800 810 820 830 840
IPGSVNAMRV LSTSTDLEAA VADALKKPVR RRYEPYGMYS DDDANSDASS VCSERSYGSR
850 860 870 880 890 900
NGGIPHYLRQ TEDVAEVLNH CASSNWSERK EGLLGLQNLL KSQRTLSRVE LKRLCEIFTR
910 920 930 940 950 960
MFADPHSKRV FSMFLETLVD FIIIHKDDLQ DWLFVLLTQL LKKMGADLLG SVQAKVQKAL
970 980 990 1000 1010 1020
DVTRDSFPFD QQFNILMRFI VDQTQTPNLK VKVAILKYIE SLARQMDPTD FVNSSETRLA
1030 1040 1050 1060 1070 1080
VSRIITWTTE PKSSDVRKAA QIVLISLFEL NTPEFTMLLG ALPKTFQDGA TKLLHNHLKN
1090 1100 1110 1120 1130 1140
SSNTGVGSPS NTIGRTPSRH PSSRTSPLTS PTNCSHGGLS PSRLWGWSAD GLSKPPPPFS
1150 1160 1170 1180 1190 1200
QPNSIPTAPS HKTLRRSYSP SMLDYDTENL NSEEIYSSLR GVTEAIEKFS FRSQEDLNEP
1210 1220 1230 1240 1250 1260
IKRDGKKDCD IVSRDGGAAS PATEGRGGSE IEGGRMALDN KTSLLNTQPP RAFPGPRARE
1270 1280 1290 1300 1310 1320
YNPYPYSDTI NTYDKTALKE AVFDDDMEQL RDVPIDHSDL VADLLKELSN HNERVEERKG
1330 1340 1350 1360 1370 1380
ALLELLKITR EDSLGVWEEH FKTILLLLLE TLGDKDHSIR ALALRVLREI LRNQPARFKN
1390 1400 1410 1420 1430 1440
YAELTIMKTL EAHKDSHKEV VRAAEEAAST LASSIHPEQC IKVLCPIIQT ADYPINLAAI
1450 1460 1470 1480 1490 1500
KMQTKVVERI TKESLLQLLV DIIPGLLQGY DNTESSVRKA SVFCLVAIYS VIGEDLKPHL
1510 1520 1530
AQLTGSKMKL LNLYIKRAQT TNSNSSSSSD VSTHS