Descriptions

The Mst1/2 kinases are cytosolic Ste20-related kinases activated by autophosphorylation. Designated class II GC kinases, Mst1 and Mst2, share 76% sequence identity and contain an N-terminal catalytic domain, followed successively by an autoinhibitory segment and a coiled-coil SARAH domain that mediates hetero- and homo-dimerisation. Mst1 and Mst2 also become activated in cells undergoing apoptosis from a variety of stimuli. The activation mechanism is unclear, however, once activated, both kinases undergo cleavage by caspase3 at sites just carboxy terminal to their catalytic domains. The resultant catalytic polypeptides display an altered substrate specificity and lack their autoinhibitory domain. The caspase-cleaved catalytic fragments are highly and constitutively active with unrestricted nuclear access.

Autoinhibitory domains (AIDs)

Target domain

26-277 (Protein kinase domain)

Relief mechanism

Cleavage

Assay

Accessory elements

162-185 (Activation loop from InterPro)

Target domain

26-277 (Protein kinase domain)

Relief mechanism

Assay

162-185 (Activation loop from InterPro)

Target domain

26-277 (Protein kinase domain)

Relief mechanism

Assay

Autoinhibited structure

Activated structure

1 structures for Q7ZUQ3

Entry ID Method Resolution Chain Position Source
AF-Q7ZUQ3-F1 Predicted AlphaFoldDB

No variants for Q7ZUQ3

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q7ZUQ3

No associated diseases with Q7ZUQ3

5 regional properties for Q7ZUQ3

Type Name Position InterPro Accession
domain Protein kinase domain 26 - 277 IPR000719
domain SARAH domain 438 - 485 IPR011524
binding_site Protein kinase, ATP binding site 32 - 55 IPR017441
domain Mst1/2 SARAH domain 438 - 485 IPR024205
domain Mst2, SARAH domain 437 - 485 IPR049568

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • The caspase-cleaved form cycles between nucleus and cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
metal ion binding Binding to a metal ion.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

7 GO annotations of biological process

Name Definition
apoptotic process A programmed cell death process which begins when a cell receives an internal (e.g. DNA damage) or external signal (e.g. an extracellular death ligand), and proceeds through a series of biochemical events (signaling pathway phase) which trigger an execution phase. The execution phase is the last step of an apoptotic process, and is typically characterized by rounding-up of the cell, retraction of pseudopodes, reduction of cellular volume (pyknosis), chromatin condensation, nuclear fragmentation (karyorrhexis), plasma membrane blebbing and fragmentation of the cell into apoptotic bodies. When the execution phase is completed, the cell has died.
camera-type eye development The process whose specific outcome is the progression of the camera-type eye over time, from its formation to the mature structure. The camera-type eye is an organ of sight that receives light through an aperture and focuses it through a lens, projecting it on a photoreceptor field.
hippo signaling The series of molecular signals mediated by the serine/threonine kinase Hippo or one of its orthologs. In Drosophila, Hippo in complex with the scaffold protein Salvador (Sav), phosphorylates and activates Warts (Wts), which in turn phosphorylates and inactivates the Yorkie (Yki) transcriptional activator. The core fly components hippo, sav, wts and mats are conserved in mammals as STK4/3 (MST1/2), SAV1/WW45, LATS1/2 and MOB1.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
protein tetramerization The formation of a protein tetramer, a macromolecular structure consisting of four noncovalently associated identical or nonidentical subunits.
regulation of MAPK cascade Any process that modulates the frequency, rate or extent of signal transduction mediated by the MAP kinase (MAPK) cascade.

17 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q5E9L6 STK4 Serine/threonine-protein kinase 4 Bos taurus (Bovine) SS
Q3SWY6 STK25 Serine/threonine-protein kinase 25 Bos taurus (Bovine) PR
Q5ZJK4 STK4 Serine/threonine-protein kinase 4 Gallus gallus (Chicken) SS
Q13043 STK4 Serine/threonine-protein kinase 4 Homo sapiens (Human) EV
Q13188 STK3 Serine/threonine-protein kinase 3 Homo sapiens (Human) SS
Q9Y6E0 STK24 Serine/threonine-protein kinase 24 Homo sapiens (Human) PR
O00506 STK25 Serine/threonine-protein kinase 25 Homo sapiens (Human) PR
Q9JI11 Stk4 Serine/threonine-protein kinase 4 Mus musculus (Mouse) PR
Q9JI10 Stk3 Serine/threonine-protein kinase 3 Mus musculus (Mouse) SS
Q9Z2W1 Stk25 Serine/threonine-protein kinase 25 Mus musculus (Mouse) PR
Q99JT2 Stk26 Serine/threonine-protein kinase 26 Mus musculus (Mouse) PR
Q99KH8 Stk24 Serine/threonine-protein kinase 24 Mus musculus (Mouse) PR
O54748 Stk3 Serine/threonine-protein kinase 3 Rattus norvegicus (Rat) SS
A4K2T0 STK4 Serine/threonine-protein kinase 4 Macaca mulatta (Rhesus macaque) PR
H2L099 gck-1 Germinal center kinase 1 Caenorhabditis elegans PR
Q9NB31 cst-1 Serine/threonine-protein kinase cst-1 Caenorhabditis elegans PR
Q6P3Q4 stk4 Serine/threonine-protein kinase 4 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MEHSVPKNKL KKLSEDSLTK QPEEVFDVLE KLGEGSYGSV FKAIHKESGQ VVAIKQVPVE
70 80 90 100 110 120
SDLQEIIKEI SIMQQCDSPY VVKYYGSYFK NTDLWIVMEY CGAGSVSDII RLRNKTLTED
130 140 150 160 170 180
EIATVLKSTL KGLEYLHFMR KIHRDIKAGN ILLNTEGHAK LADFGVAGQL TDTMAKRNTV
190 200 210 220 230 240
IGTPFWMAPE VIQEIGYNCV ADIWSLGITS IEMAEGKPPY ADIHPMRAIF MIPTNPPPTF
250 260 270 280 290 300
RKPEHWSDDF TDFVKKCLVK NPEQRATATQ LLQHPFIVGA KPVSILRDLI TEAMDMKAKR
310 320 330 340 350 360
QQEQQRELEE DDENSEEEVE VDSHTMVKSG SESAGTMRAT GTMSDGAQTM IEHGSTMLES
370 380 390 400 410 420
NLGTMVINSD DEEEEEDLGS MRRNPTSQQI QRPSFMDYFD KQDSNKAQEG FNHNQQDPCL
430 440 450 460 470 480
ISKTAFPDNW KVPQDGDFDF LKNLDFEELQ MRLTALDPMM EREIEELRQR YTAKRQPILD
490
AMDAKKRRQQ NF