Q7TSU1

PR
Gene name
Arfgef2 (Arfgep2, Big2)
Protein name
Brefeldin A-inhibited guanine nucleotide-exchange protein 2
Names
Brefeldin A-inhibited GEP 2, ADP-ribosylation factor guanine nucleotide-exchange factor 2
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:296380
EC number
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

181-381 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q7TSU1

Entry ID Method Resolution Chain Position Source
AF-Q7TSU1-F1 Predicted AlphaFoldDB

1 variants for Q7TSU1

Variant ID(s) Position Change Description Diseaes Association Provenance
rs8166373 1763 R>L No EVA

No associated diseases with Q7TSU1

11 regional properties for Q7TSU1

Type Name Position InterPro Accession
domain B-box-type zinc finger 84 - 125 IPR000315
domain Zinc finger, RING-type 16 - 57 IPR001841
domain B30.2/SPRY domain 276 - 475 IPR001870
domain SPRY domain 346 - 474 IPR003877
domain Butyrophylin-like, SPRY domain 292 - 309 IPR003879-1
domain Butyrophylin-like, SPRY domain 331 - 355 IPR003879-2
domain Butyrophylin-like, SPRY domain 361 - 374 IPR003879-3
domain Butyrophylin-like, SPRY domain 405 - 429 IPR003879-4
domain SPRY-associated 293 - 345 IPR006574
conserved_site Zinc finger, RING-type, conserved site 31 - 40 IPR017907
domain Zinc finger, RING-type, eukaryotic 16 - 54 IPR027370

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Membrane
  • Golgi apparatus
  • Cytoplasm, perinuclear region
  • Golgi apparatus, trans-Golgi network
  • Endosome
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Cell projection, dendrite
  • Cytoplasmic vesicle
  • Synapse
  • Cytoplasm, cytoskeleton
  • Translocates from cytoplasm to membranes upon cAMP treatment
  • Localized in recycling endosomes (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

14 GO annotations of cellular component

Name Definition
anchoring junction A cell junction that mechanically attaches a cell (and its cytoskeleton) to neighboring cells or to the extracellular matrix.
asymmetric synapse A type of synapse occurring between an axon and a dendritic spine or dendritic shaft. Asymmetric synapses, the most abundant synapse type in the central nervous system, involve axons that contain predominantly spherical vesicles and contain a thickened postsynaptic density. Most or all synapses of this type are excitatory.
axonemal microtubule A microtubule in the axoneme of a eukaryotic cilium or flagellum; an axoneme contains nine modified doublet microtubules, which may or may not surround a pair of single microtubules.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic vesicle A vesicle found in the cytoplasm of a cell.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendritic spine A small, membranous protrusion from a dendrite that forms a postsynaptic compartment, typically receiving input from a single presynapse. They function as partially isolated biochemical and an electrical compartments. Spine morphology is variable:they can be thin, stubby, mushroom, or branched, with a continuum of intermediate morphologies. They typically terminate in a bulb shape, linked to the dendritic shaft by a restriction. Spine remodeling is though to be involved in synaptic plasticity.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
recycling endosome An organelle consisting of a network of tubules that functions in targeting molecules, such as receptors transporters and lipids, to the plasma membrane.
symmetric synapse A synapse that lacks an electron dense postsynaptic specialization. In vertebtrates, these occur primarily on dendrite shafts and neuronal cell bodies and involve persynapses containing clusters of predominantly flattened or elongated vesicles and are typcially inhibitory.
trans-Golgi network The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.

4 GO annotations of molecular function

Name Definition
GABA receptor binding Binding to a gamma-aminobutyric acid (GABA, 4-aminobutyrate) receptor.
guanyl-nucleotide exchange factor activity Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions.
myosin binding Binding to a myosin; myosins are any of a superfamily of molecular motor proteins that bind to actin and use the energy of ATP hydrolysis to generate force and movement along actin filaments.
protein kinase A regulatory subunit binding Binding to one or both of the regulatory subunits of protein kinase A.

9 GO annotations of biological process

Name Definition
endomembrane system organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the endomembrane system.
endosome organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of endosomes.
exocytosis A process of secretion by a cell that results in the release of intracellular molecules (e.g. hormones, matrix proteins) contained within a membrane-bounded vesicle. Exocytosis can occur either by full fusion, when the vesicle collapses into the plasma membrane, or by a kiss-and-run mechanism that involves the formation of a transient contact, a pore, between a granule (for exemple of chromaffin cells) and the plasma membrane. The latter process most of the time leads to only partial secretion of the granule content. Exocytosis begins with steps that prepare vesicles for fusion with the membrane (tethering and docking) and ends when molecules are secreted from the cell.
Golgi to plasma membrane transport The directed movement of substances from the Golgi to the plasma membrane in transport vesicles that move from the trans-Golgi network to the plasma membrane, where they fuse and release their contents by exocytosis.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
positive regulation of tumor necrosis factor production Any process that activates or increases the frequency, rate or extent of tumor necrosis factor production.
protein transport The directed movement of proteins into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
receptor recycling The process that results in the return of receptor molecules to an active state and an active cellular location after they have been stimulated by a ligand. An active state is when the receptor is ready to receive a signal.
regulation of ARF protein signal transduction Any process that modulates the frequency, rate or extent of ARF protein signal transduction.

11 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
O46382 ARFGEF1 Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Bos taurus (Bovine) PR
Q9Y6D5 ARFGEF2 Brefeldin A-inhibited guanine nucleotide-exchange protein 2 Homo sapiens (Human) PR
Q9Y6D6 ARFGEF1 Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Homo sapiens (Human) PR
A2A5R2 Arfgef2 Brefeldin A-inhibited guanine nucleotide-exchange protein 2 Mus musculus (Mouse) PR
G3X9K3 Arfgef1 Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Mus musculus (Mouse) PR
D4A631 Arfgef1 Brefeldin A-inhibited guanine nucleotide-exchange protein 1 Rattus norvegicus (Rat) PR
A0A0G2JUG7 Iqsec1 IQ motif and SEC7 domain-containing protein 1 Rattus norvegicus (Rat) SS
Q76M68 Iqsec3 IQ motif and SEC7 domain-containing protein 3 Rattus norvegicus (Rat) SS
P63035 Cyth2 Cytohesin-2 Rattus norvegicus (Rat) SS
P97696 Cyth3 Cytohesin-3 Rattus norvegicus (Rat) SS
P97694 Cyth1 Cytohesin-1 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MQESQTKSMF VSRALEKILA DKEVKRPQHS QLRRACQVAL DEIKAELEKQ RLGAAAPPKA
70 80 90 100 110 120
NFIEADKYFL PFELACQSKS PRVVSTSLDC LQKLIAYGHI TGNAPDSGAP GKRLIDRIVE
130 140 150 160 170 180
TVCNCFQGPQ TDEGVQLQII KALLTAVTSP HIEIHEGTIL QTVRTCYNIY LASKNLINQT
190 200 210 220 230 240
TAKATLTQML NVIFTRMENQ VLQEARELEK PIQSKPQSPV IQATAGSPKF SRLKQSQAQS
250 260 270 280 290 300
KPTTPEKTEL PNGDHARSSL GKVNSENGEA HRERGSSISG RAEPSGGSDN GAQEVVKDIL
310 320 330 340 350 360
EDVVTSAVKE AAEKQGLPEP DQAPGVPECQ ECTVPPAVDE NSQTNGIADD RQSLSSADNL
370 380 390 400 410 420
EPDAQGHPVA ARFSHILQKD AFLVFRSLCK LSMKPLGEGP PDPKSHELRS KVVSLQLLLS
430 440 450 460 470 480
VLQNAGPVFR SHEMFVTAIK QYLCVALSKN GVSSVPDVFE LSLAIFLTLL SNFKMHLKMQ
490 500 510 520 530 540
IEVFFKEIFL NILETSTSSF EHRWMVIQTL TRICADAQCV VDIYVNYDCD LNAANIFERL
550 560 570 580 590 600
VNDLSKIAQG RSGHELGMTP LQELSLRKKG LECLVSILKC MVEWSKDLYV NPNHQATLGQ
610 620 630 640 650 660
ERLPDQEMGD GKGLDMARRC SVTSVESTVS SGTQTAIPDD PEQFEVIKQQ KEIIEHGIEL
670 680 690 700 710 720
FNKKPKRGIQ FLQEQGMLGA AVEDIAQFLH QEERLDSTQV GEFLGDSTRF NKEVMYAYVD
730 740 750 760 770 780
QLDFCEKEFV SALRTFLEGF RLPGEAQKID RLMEKFAARY IECNQGQTLF ASADTAYVLA
790 800 810 820 830 840
YSIIMLTTDL HSPQVKNKMT KEQYIKMNRG INDSKDLPEE YLSSIYEEIE GKKIAMKETK
850 860 870 880 890 900
EHTMATKSTK QNVASEKQRR LLYNVEMEQM AKTAKALMEA VSHAKAPFTS ATHLDHVRPM
910 920 930 940 950 960
FKLVWTPLLA AYSIGLQNCD DTEVASLCLE GIRCAVRIAC IFGMQLERDA YVQALARFSL
970 980 990 1000 1010 1020
LTASSSITEM KQKNIDTIKT LITVAHTDGN YLGNSWHEIL KCISQLELAQ LIGTGVKTRY
1030 1040 1050 1060 1070 1080
LSGSGREREG SLKGHSLAGE EFMGLGLGNL VSGGVDKRQM ASFQESVGET SSQSVVVAVD
1090 1100 1110 1120 1130 1140
RIFTGSTRLD GNAIVDFVRW LCAVSMDELA SPHHPRMFSL QKIVEISYYN MNRIRLQWSR
1150 1160 1170 1180 1190 1200
IWHVIGDHFN KVGCNPNEDV AIFAVDSLRQ LSMKFLEKGE LANFRFQKDF LRPFEHIMKK
1210 1220 1230 1240 1250 1260
NRSPTIRDMV IRCIAQMVSS QAANIRSGWK NIFAVFHQAA SDHDGNIVEL AFQTTGHIVS
1270 1280 1290 1300 1310 1320
TIFQHHFPAA IDSFQDAVKC LSEFACNAAF PDTSMEAIRL IRFCGKYVSE RPRVLQEYTS
1330 1340 1350 1360 1370 1380
DDMNVAPGDR VWVRGWFPIL FELSCIINRC KLDVRTRGLT VMFEIMKSYG HTFAKHWWQD
1390 1400 1410 1420 1430 1440
LFRIVFRIFD NMKLPEQQSE KSEWMTTTCN HALYAICDVF TQFYEALHEV LLSDVFAQLQ
1450 1460 1470 1480 1490 1500
WCVKQDNEQL ARSGTNCLEN LVISNGEKFS PAVWDETCNC MLDIFRTTIP HVLLTWRPAG
1510 1520 1530 1540 1550 1560
MEEEVSDRHL DVDLDRQSLS SIDRNASERG QSQLSNPTDD SWKGAPYANQ KLLASLLIKC
1570 1580 1590 1600 1610 1620
VVQLELIQTI DNIVFYPATS KKEDAEHMVA AQQDTLDADI HIETENQGMY KFMSSQHLFK
1630 1640 1650 1660 1670 1680
LLDCLQESHS FSKAFNSNYE QRTVLWRAGF KGKSKPNLLK QETSSLACCL RILFRMYVDE
1690 1700 1710 1720 1730 1740
NRRDSWGEIQ QRLLTVCSEA LAYFITVNSE SHREAWTSLL LLLLTKTLKI NDEKFKAHAS
1750 1760 1770 1780 1790
VYYPYLCEMM QFDLIPELRA VLRKFFLRIG LVYKIWVPEE PSQVPAASTA W