Q7TPK6

SS
Gene name
Wnk4 (Prkwnk4)
Protein name
Serine/threonine-protein kinase WNK4
Names
EC 2.7.11.1 , Protein kinase lysine-deficient 4 , Protein kinase with no lysine 4
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:287715
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other protein kinases. The activity of the Wnk1 kinase domain is controlled by its autoinhibitory domain, which is relieved through specific mutations of key residues in the domain. Activation of Wnk1 requires autophosphorylaiton of Ser382 within its activation loop, and phosphorylation of Ser378 enhances its kinase activity.

Autoinhibitory domains (AIDs)

463-486 (Autoinhibitory region) SS

Target domain

171-429 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

317-338 (Activation loop from InterPro)

Target domain

171-429 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q7TPK6

Entry ID Method Resolution Chain Position Source
AF-Q7TPK6-F1 Predicted AlphaFoldDB

1 variants for Q7TPK6

Variant ID(s) Position Change Description Diseaes Association Provenance
rs13452276 1107 S>I No EVA

No associated diseases with Q7TPK6

5 regional properties for Q7TPK6

Type Name Position InterPro Accession
domain Protein kinase domain 89 - 382 IPR000719
domain AGC-kinase, C-terminal 383 - 455 IPR000961
active_site Serine/threonine-protein kinase, active site 208 - 220 IPR008271
binding_site Protein kinase, ATP binding site 95 - 118 IPR017441
domain Protein kinase, C-terminal 401 - 445 IPR017892

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cell junction, tight junction
  • Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct
  • WNK4 is part of the tight junction complex
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
bicellular tight junction An occluding cell-cell junction that is composed of a branching network of sealing strands that completely encircles the apical end of each cell in an epithelial sheet; the outer leaflets of the two interacting plasma membranes are seen to be tightly apposed where sealing strands are present. Each sealing strand is composed of a long row of transmembrane adhesion proteins embedded in each of the two interacting plasma membranes.
cell body The portion of a cell bearing surface projections such as axons, dendrites, cilia, or flagella that includes the nucleus, but excludes all cell projections.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
protein-containing complex A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chloride ion binding Binding to a chloride ion (Cl-).
ion channel inhibitor activity Binds to and stops, prevents, or reduces the activity of an ion channel.
potassium channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a potassium channel.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

28 GO annotations of biological process

Name Definition
aldosterone secretion The regulated release of aldosterone into the circulatory system. Aldosterone is a pregnane-based steroid hormone produced by the outer-section (zona glomerulosa) of the adrenal cortex in the adrenal gland, and acts on the distal tubules and collecting ducts of the kidney to cause the conservation of sodium, secretion of potassium, increased water retention, and increased blood pressure. The overall effect of aldosterone is to increase reabsorption of ions and water in the kidney.
calcium ion homeostasis Any process involved in the maintenance of an internal steady state of calcium ions within an organism or cell.
cellular response to xenobiotic stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organism exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
chloride transport The directed movement of chloride into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
distal tubule morphogenesis The process in which the anatomical structures of a distal tubule are generated and organized. The distal tubule is a nephron tubule that begins at the macula densa and extends to the connecting tubule.
ERK1 and ERK2 cascade An intracellular protein kinase cascade containing at least ERK1 or ERK2 (MAPKs), a MEK (a MAPKK) and a MAP3K. The cascade may involve 4 different kinases, as it can also contain an additional tier
gene expression The process in which a gene's sequence is converted into a mature gene product (protein or RNA). This includes the production of an RNA transcript and its processing, translation and maturation for protein-coding genes.
inflammatory response The immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents. The process is characterized by local vasodilation, extravasation of plasma into intercellular spaces and accumulation of white blood cells and macrophages.
intracellular chloride ion homeostasis A homeostatic process involved in the maintenance of a steady state level of chloride ions within a cell.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
macrophage activation A change in morphology and behavior of a macrophage resulting from exposure to a cytokine, chemokine, cellular ligand, or soluble factor.
monoatomic ion homeostasis Any process involved in the maintenance of an internal steady state of monoatomic ions within an organism or cell. Monatomic ions (also called simple ions) are ions consisting of exactly one atom.
negative regulation of pancreatic juice secretion Any process that decreases the rate, frequency or extent of pancreatic juice secretion, the regulated release of pancreatic juice by the exocrine pancreas into the upper part of the intestine.
negative regulation of protein localization to plasma membrane Any process that stops, prevents or reduces the frequency, rate or extent of protein localization to plasma membrane.
negative regulation of sodium ion transport Any process that decreases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
positive regulation of potassium ion import across plasma membrane Any process that activates or increases the frequency, rate or extent of potassium ion import across the plasma membrane.
potassium ion transmembrane transport A process in which a potassium ion is transported from one side of a membrane to the other.
protein kinase C signaling A series of reactions, mediated by the intracellular serine/threonine kinase protein kinase C, which occurs as a result of a single trigger reaction or compound.
protein localization Any process in which a protein is transported to, or maintained in, a specific location.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of blood pressure Any process that modulates the force with which blood travels through the circulatory system. The process is controlled by a balance of processes that increase pressure and decrease pressure.
regulation of potassium ion export across plasma membrane Any process that modulates the frequency, rate or extent of potassium ion export across the plasma membrane.
renal sodium ion absorption A renal system process in which sodium ions are taken up from the collecting ducts and proximal and distal loops of the nephron. In non-mammalian species, absorption may occur in related structures.
renal sodium ion transport The directed movement of sodium ions (Na+) by the renal system.
response to dietary excess The physiological process in which dietary excess is sensed by the central nervous system, resulting in a reduction in food intake and increased energy expenditure.
response to xenobiotic stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
signal transduction The cellular process in which a signal is conveyed to trigger a change in the activity or state of a cell. Signal transduction begins with reception of a signal (e.g. a ligand binding to a receptor or receptor activation by a stimulus such as light), or for signal transduction in the absence of ligand, signal-withdrawal or the activity of a constitutively active receptor. Signal transduction ends with regulation of a downstream cellular process, e.g. regulation of transcription or regulation of a metabolic process. Signal transduction covers signaling from receptors located on the surface of the cell and signaling via molecules located within the cell. For signaling between cells, signal transduction is restricted to events at and within the receiving cell.
sodium ion transmembrane transport A process in which a sodium ion is transported from one side of a membrane to the other by means of some agent such as a transporter or pore.

16 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9Y3S1 WNK2 Serine/threonine-protein kinase WNK2 Homo sapiens (Human) SS
Q9H4A3 WNK1 Serine/threonine-protein kinase WNK1 Homo sapiens (Human) SS
Q9BYP7 WNK3 Serine/threonine-protein kinase WNK3 Homo sapiens (Human) SS
Q96J92 WNK4 Serine/threonine-protein kinase WNK4 Homo sapiens (Human) SS
P83741 Wnk1 Serine/threonine-protein kinase WNK1 Mus musculus (Mouse) SS
Q3UH66 Wnk2 Serine/threonine-protein kinase WNK2 Mus musculus (Mouse) SS
Q80XP9 Wnk3 Serine/threonine-protein kinase WNK3 Mus musculus (Mouse) SS
Q80UE6 Wnk4 Serine/threonine-protein kinase WNK4 Mus musculus (Mouse) SS
Q6R2V0 WNK1 Serine/threonine-protein kinase WNK1 Sus scrofa (Pig) SS
Q9JIH7 Wnk1 Serine/threonine-protein kinase WNK1 Rattus norvegicus (Rat) EV
X5M5N0 wnk-1 Serine/threonine-protein kinase WNK Caenorhabditis elegans SS
Q8RXE5 WNK10 Probable serine/threonine-protein kinase WNK10 Arabidopsis thaliana (Mouse-ear cress) PR
Q944Q0 WNK8 Serine/threonine-protein kinase WNK8 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LVL5 WNK4 Probable serine/threonine-protein kinase WNK4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STK6 WNK3 Probable serine/threonine-protein kinase WNK3 Arabidopsis thaliana (Mouse-ear cress) PR
Q8LST2 WNK7 Probable serine/threonine-protein kinase WNK7 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MLAPRNTETG VHMSQTEADL ALRPSPSLTS MGPTRLGPPP RRVRRFSGKA EPRPRSSRPS
70 80 90 100 110 120
RRSSVDLGLL SSWSQPASLL PEPPDPPDSA GPMRSPPSNS KEHPEGTWTG AAPVKAADSA
130 140 150 160 170 180
CPELTVSSGG PGSREPPRVP DAAARERRRE QEEKEDTETQ AVATSPDGRY LKFDIEIGRG
190 200 210 220 230 240
SFKTVYRGLD TDTTVEVAWC ELQTRKLSRA ERQRFSEEVE MLKGLQHPNI VRFYDSWKSV
250 260 270 280 290 300
LRGQVCIVLV TELMTSGTLK TYLRRFREMK PRVLQRWSRQ ILRGLHFLHS RVPPILHRDL
310 320 330 340 350 360
KCDNVFITGP SGSVKIGDLG LATLKRASFA KSVIGTPEFM APEMYEEKYD EAVDVYAFGM
370 380 390 400 410 420
CMLEMATSEY PYSECQNAAQ IYRKVTSGTK PNSFYKVKMP EVKEIIEGCI RTDKNERFTI
430 440 450 460 470 480
QDLLTHAFFR EERGVHVELA EEDDGEKPGL KLWLRMEDAR RGGRPRDNQA IEFLFQLGRD
490 500 510 520 530 540
AAEEVAQEMV ALGLVCEADY QPVARAVRER VAAIQRKREK LRKARELEVL PPDSGPPPAT
550 560 570 580 590 600
VSMTPGPPSA FPPEPEEPEA DQHQSFLFRH ASYSSTTSDC ETDGYLSSSG FLDASDPALQ
610 620 630 640 650 660
PPGGMPSSPA EPHLCLPSGF ALSIPRSGPG SDFSPGDSYA SDAASGLSDM GEGGQMRKNP
670 680 690 700 710 720
VKTLRRRPRS RLRVTSVSDQ SDRVVECQLQ THNSKMVTFR FDLDGDSPEE IAAAMVYNEF
730 740 750 760 770 780
ILPSERDGFL SRIREIIQRV ETLLKRDAGP SEATEDALSP QEEPAAMPAL PGPSDAELQR
790 800 810 820 830 840
SISPEQRSWA AFSTSPSSPG TPLSPGTPFS PGTPPVFPCP IFPITSPSCH PYPFSQVSSN
850 860 870 880 890 900
PCPQAPSSLL PSSSGASQVP FPSPSLPTSS PLPFSPSYPQ VPLHPASLPT CPSPPPLPST
910 920 930 940 950 960
TAAPLLSLAS AFSLAVMTVA QSLLSPSPGL LSQSPPAPPG PLPSMPLPLA SCDQESLSAQ
970 980 990 1000 1010 1020
TAETENEASR NPAQPLLGDA RLAPISEEGK PQLVGRFQVT SSKEPAEPPL QPASPTLSRS
1030 1040 1050 1060 1070 1080
LKLPTPQLTS ESSDTEDSAA GGPETREALA ESDRAAEGLG VAIDEEKDEG KEPQIGGSSP
1090 1100 1110 1120 1130 1140
ILSQPSPVWM NYSYSSLCLS SEESESSGED EEFWAELQNL RQKHLSEVEA LQTLQKKEIE
1150 1160 1170 1180 1190 1200
DLYSRLGKQP PPGIVAPAAM LSCRQRRLSK GSFPTSRRNS LQRSDLPGPG IMRRNSLSGS
1210 1220
STGSQEQRAS KGVTFAGDVG RM