Q70AM4

EV
Gene name
Kif13b (kif13B)
Protein name
Kinesin 13B
Names
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:305967
EC number
Protein Class
STAR-RELATED LIPID TRANSFER PROTEIN 9 (PTHR47117)

Descriptions

Kinesin-3 KIF13B autoinhibition involves the coiled-coil 1 (CC1) segment, which interacts with both the neck coil (NC) and the motor domain (MD). This interaction not only inhibits the NC-mediated dimerization, but also prevents the ADP release from the MD, effectively inhibiting the motor activity.

Autoinhibitory domains (AIDs)

390-432 (Coiled-coil 1) EV

Target domain

1-389 (Motor domain and neck coil)

Relief mechanism

Partner binding

Assay

Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

0 structures for Q70AM4

Entry ID Method Resolution Chain Position Source

No variants for Q70AM4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q70AM4

No associated diseases with Q70AM4

8 regional properties for Q70AM4

Type Name Position InterPro Accession
domain Forkhead-associated (FHA) domain 472 - 535 IPR000253
domain CAP Gly-rich domain 1643 - 1708 IPR000938
domain Kinesin motor domain 3 - 361 IPR001752
conserved_site Kinesin motor domain, conserved site 247 - 258 IPR019821
domain Kinesin-like KIF1-type 756 - 802 IPR022140
domain Kinesin-like 1003 - 1061 IPR022164-1
domain Kinesin-like 1214 - 1278 IPR022164-2
domain Kinesin-associated 390 - 469 IPR032405

Functions

Description
EC Number
Subcellular Localization
PANTHER Family PTHR47117 STAR-RELATED LIPID TRANSFER PROTEIN 9
PANTHER Subfamily PTHR47117:SF3 KINESIN FAMILY MEMBER 14-LIKE
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
axon The long process of a neuron that conducts nerve impulses, usually away from the cell body to the terminals and varicosities, which are sites of storage and release of neurotransmitter.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinesin complex Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.

6 GO annotations of molecular function

Name Definition
14-3-3 protein binding Binding to a 14-3-3 protein. A 14-3-3 protein is any of a large family of approximately 30kDa acidic proteins which exist primarily as homo- and heterodimers within all eukaryotic cells, and have been implicated in the modulation of distinct biological processes by binding to specific phosphorylated sites on diverse target proteins, thereby forcing conformational changes or influencing interactions between their targets and other molecules. Each 14-3-3 protein sequence can be roughly divided into three sections
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule motor activity A motor activity that generates movement along a microtubule, driven by ATP hydrolysis.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.

3 GO annotations of biological process

Name Definition
cytoskeleton-dependent intracellular transport The directed movement of substances along cytoskeletal fibers such as microfilaments or microtubules within a cell.
microtubule-based movement A microtubule-based process that results in the movement of organelles, other microtubules, or other cellular components. Examples include motor-driven movement along microtubules and movement driven by polymerization or depolymerization of microtubules.
regulation of axonogenesis Any process that modulates the frequency, rate or extent of axonogenesis, the generation of an axon, the long process of a neuron.

3 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q9H1H9 KIF13A Kinesin-like protein KIF13A Homo sapiens (Human) SS
Q9NQT8 KIF13B Kinesin-like protein KIF13B Homo sapiens (Human) EV
Q9EQW7 Kif13a Kinesin-like protein KIF13A Mus musculus (Mouse) SS
10 20 30 40 50 60
MGDSKVKVAV RVRPMNRREI DLHTKCVVDV EANKVILNPI NTNLSKGDAR GQPKIFAYDH
70 80 90 100 110 120
CFWSMDESVR EKYAGQDDVF KCLGENILQN AFDGYNACIF AYGQTGSGKS YTMMGTADQP
130 140 150 160 170 180
GLIPRLCSGL FERTQKEENE EQSFKVEVSY MEIYNEKVRD LLDPKGSRQT LKVREHSVLG
190 200 210 220 230 240
PYVDGLSKLA VTSYKDIESL MSEGNKSRTV AATNMNEESS RSHAVFKITL THTLYDVKSG
250 260 270 280 290 300
TSGEKVGKLS LVDLAGSERA TKTGAAGDRL KEGSNINKSL TTLGLVISAL ADQGAGKNKN
310 320 330 340 350 360
KFVPYRDSVL TWLLKDSLGG NSKTAMVATV SPAADNYDET LSTLRYADRA KHIINHAVVN
370 380 390 400 410 420
EDPNARIIRD LREEVEKLRE QLTKAEAMKS PELKDRLEES EKLIQEMTVT WEEKLRKTEE
430 440 450 460 470 480
IAQERQKQLE SLGISLQTSG IKVGDDKCFL VNLNADPALN ELLVYYLKEH TLIGSANSQD
490 500 510 520 530 540
IQLCGMGILP EHGIIDIMPE GQVMLTPQKN TRTFVNGSSV SSPIQLHHGD RILWGNNHFF
550 560 570 580 590 600
RLNLPKKKKK AEREDEEREA SLKNDSSSEQ LDADGDSSSE VSSEINFNFE YAQMEVTMKA
610 620 630 640 650 660
LGSNDPMQSI LSSLEQQHEE EKRSALERQR LMYEHELEQL RRRLSPERQN CRGVDRLSFH
670 680 690 700 710 720
SPSAQQRLRQ WAEEREATLN NSLMRLREQI VKANLLVREA SYIAEELDKR TEYKVTLQIP
730 740 750 760 770 780
ASSLDANRKR GSLLSEPAIQ VRRKGKGKQI WSLEKLENRL LDMRDLYQEW KECEEDSPVS
790 800 810 820 830 840
RSYFKRADPF YDEQENHSLI GVANVFLETL FYDVKLQYAV PIINQKGEVA GRLHVEVMRL
850 860 870 880 890 900
SGAIGERIAG GDDPTEVSSE KEVQENRLVC MVKILQATGL PQHLCHFVFC KYDFWDQQEP
910 920 930 940 950 960
VTVAPEVDTS SSPTSKEPQC MVVFDHCSEF SVNITEDFIE YLSEGALAIE VYGHKMNDPR
970 980 990 1000 1010 1020
KNPALWDLGI IQAKTRSLRD RWSEVTRKLE FWVQILEQNE NGEYCPVEVI AAKDVPTGGI
1030 1040 1050 1060 1070 1080
FQLRQGQSRR VQVEVKSVQE SGTLPLMEEC ILSVGIGCVK VRPLRSPKTH ENIHEEEEDM
1090 1100 1110 1120 1130 1140
DSYQDRDLER LRRKWLNALT KRQEYLDQQL QKLVSKHDKT EDDADREAQL LEMRLTLTEE
1150 1160 1170 1180 1190 1200
RNAVMVPSAG SGIPGAPAEW TPVPGMEAHI PVVFLDLNAD DFSSQDNLDD PEAGWDATLT
1210 1220 1230 1240 1250 1260
GEEEEEFFEL QIVKHHDGEV KAEASWDSAV HNCPQLSKGT PADERVFLIL RVAVQLSHPA
1270 1280 1290 1300 1310 1320
DMQLVLRKRI CVHVHGRQGF AQSLLKKMTH RSSIPGCGVT FEIVSNIPEV DAQGVEEREA
1330 1340 1350 1360 1370 1380
LARMAANVEN AASADSEACI EKYLRSVLAV ENLLTLDRLR QEVAVKEQLT GKGKLNRRSI
1390 1400 1410 1420 1430 1440
SSPSMNRLSG SRQELSPSHS LSSNKPVPRI LVQPTFSDAR ATRTEEAQQG SPGPSGALES
1450 1460 1470 1480 1490 1500
MVKMAAPTVK ICDKPVRVSS PPSTMVVTQP QEGQDGPPSP LSEASSGYFS HSVSTATLSE
1510 1520 1530 1540 1550 1560
TLTLGLDTTG LGSQTPGSPP ALCQVTQEPE LAFLSCTQSH PTDPEEPHIP PATPTQSTEL
1570 1580 1590 1600 1610 1620
EVPRPPLLSD PTPAVPTSPF RIRKVRPSEL TSFTGMLGGA SSGAQEDPVV SEDPSHARGQ
1630 1640 1650 1660 1670 1680
TLGRLEVTSD SEDASEVPEW LREGEYVVVG TNKTGIVRYI GPTDFQEGTW IGVELDLPAG
1690 1700 1710 1720 1730 1740
KNDGSIGGKQ YFRCNPGYGL LVRPGRVRRA AGTGRRRSSG LQPQGAPEAR RSATISGSAT
1750 1760
NLASLTAALA KGDRSYKNPE NRKSWAS