Q6TV19

SS
Gene name
dicer1
Protein name
Endoribonuclease Dicer
Names
EC 3.1.26.3
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
KEGG Pathway
dre:324724
EC number
3.1.26.3: Endoribonucleases producing 5'-phosphomonoesters
Protein Class

Descriptions

Dicer, a member of the Ribonuclease III family of enzymes, processes double-stranded RNA substrates into ~21-27 nucleotide products that trigger sequence-directed gene silencing by RNA interference (RNAi). The N-terminal domain of Dicer, which is homologous to DExD/H-box helicases, substantially attenuates the rate of substrate cleavage. Deletion or mutation of this domain activates human Dicer. Modest stimulation of catalysis by the full-length Dicer enzyme was observed in the presence of the TAR-RNA binding protein (TRBP), which physically interacts with the DExD/H-box domain. Thus, the DExD/H-box domain likely disrupts the functionality of the Dicer active site until a structural rearrangement occurs, perhaps upon assembly with its molecular partners

Autoinhibitory domains (AIDs)

0-590 (DExD/H-box helicase domain) SS

Target domain

1258-1385 (RNase IIIa domain);1609-1767 (RNase IIIb domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q6TV19

Entry ID Method Resolution Chain Position Source
AF-Q6TV19-F1 Predicted AlphaFoldDB

No variants for Q6TV19

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q6TV19

No associated diseases with Q6TV19

11 regional properties for Q6TV19

Type Name Position InterPro Accession
domain Ribonuclease III domain 1277 - 1572 IPR000999-1
domain Ribonuclease III domain 1609 - 1790 IPR000999-2
domain Helicase, C-terminal domain-like 419 - 588 IPR001650
domain PAZ domain 877 - 1052 IPR003100
domain Dicer dimerisation domain 616 - 708 IPR005034
domain DEAD/DEAH box helicase domain 36 - 195 IPR011545
domain Helicase superfamily 1/2, ATP-binding domain 30 - 218 IPR014001
domain Double-stranded RNA-binding domain 1792 - 1857 IPR014720
domain Dicer, double-stranded RNA-binding domain 1773 - 1861 IPR044441
domain Dicer, platform domain 747 - 872 IPR048512
domain Dicer, partner-binding domain 254 - 352 IPR048513

Functions

Description
EC Number 3.1.26.3 Endoribonucleases producing 5'-phosphomonoesters
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
RISC complex A ribonucleoprotein complex that contains members of the Argonaute family of proteins, small interfering RNAs (siRNAs) or microRNAs (miRNAs), and miRNA or siRNA-complementary mRNAs, in addition to a number of accessory factors. The RISC complex is involved in posttranscriptional repression of gene expression through downregulation of translation or induction of mRNA degradation.
RISC-loading complex A trimeric protein complex required for the formation of a mature RNA-induced silencing complex (RISC). In humans the complex is composed of the endonuclease Dicer (DICER1), TRBP (TARBP2) and the Argonaute protein Ago2 (EIF2C2/AGO2). Within the complex, Dicer and TRBP are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto Ago2. Ago2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from Dicer and TRBP. This complex has endoribonuclease activity.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
deoxyribonuclease I activity Catalysis of the endonucleolytic cleavage of DNA to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end products.
helicase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
pre-miRNA binding Binding to a precursor microRNA (pre-miRNA) transcript, a stem-loop-containing precursor of microRNA.
ribonuclease III activity Catalysis of the endonucleolytic cleavage of RNA with 5'-phosphomonoesters and 3'-OH termini; makes two staggered cuts in both strands of dsRNA, leaving a 3' overhang of 2 nt.
RNA binding Binding to an RNA molecule or a portion thereof.

13 GO annotations of biological process

Name Definition
apoptotic DNA fragmentation The cleavage of DNA during apoptosis, which usually occurs in two stages
cranial skeletal system development The process whose specific outcome is the progression of a cranial skeletal system over time, from its formation to the mature structure. The cranial skeletal system is the skeletal subdivision of the head, and includes the skull (cranium plus mandible), pharyngeal and/or hyoid apparatus.
global gene silencing by mRNA cleavage A posttranscriptional gene silencing pathway that involves the cleavage of mRNAs in a non-gene-specific manner.
heterochromatin formation An epigenetic gene silencing mechanism in which chromatin is compacted into heterochromatin, resulting in a chromatin conformation refractory to transcription. This process starts with heterochromatin nucleation, its spreading, and ends with heterochromatin boundary formation.
melanocyte differentiation The process in which a relatively unspecialized cell acquires specialized features of a melanocyte.
miRNA processing A process leading to the generation of a functional miRNA. Includes the cleavage of stem-loop RNA precursors into microRNAs (miRNAs). miRNAs are a class of small RNAs that primarily silence genes by blocking the translation of mRNA transcripts into protein, or by increasing the degradation of non-protein-coding RNA transcripts.
miRNA-mediated gene silencing by mRNA destabilization An RNA interference pathway in which microRNAs (miRNAs) direct the cleavage of target mRNAs. Once incorporated into a RNA-induced silencing complex (RISC), a miRNA base pairing with near-perfect complementarity to the target mRNA will typically direct targeted endonucleolytic cleavage of the mRNA. Many plant miRNAs downregulate gene expression through this mechanism.
miRNA-mediated post-transcriptional gene silencing A post-transcriptional gene silencing pathway in which regulatory microRNAs (miRNAs) elicit silencing of specific target genes. miRNAs are endogenous 21-24 nucleotide small RNAs processed from stem-loop RNA precursors (pre-miRNAs). Once incorporated into a RNA-induced silencing complex (RISC), miRNAs can downregulate protein production by either of two posttranscriptional mechanisms
neural crest cell development The process aimed at the progression of a neural crest cell over time, from initial commitment of the cell to its specific fate, to the fully functional differentiated cell.
pre-miRNA processing A process involved in the conversion of a pre-microRNA transcript into a mature microRNA molecule.
regulation of neurogenesis Any process that modulates the frequency, rate or extent of neurogenesis, the generation of cells in the nervous system.
siRNA processing A process leading to the generation of a functional small interfering RNA (siRNA). Includes the cleavage of double-stranded RNA to form small interfering RNA molecules (siRNAs) of 21-23 nucleotides. May also include amplification of the siRNA by RNA-directed RNA polymerase.
ventricular system development The process whose specific outcome is the progression of the brain ventricular system over time, from its formation to the mature structure. The brain ventricular system consists of four communicating cavities within the brain that are continuous with the central canal of the spinal cord. These cavities include two lateral ventricles, the third ventricle and the fourth ventricle. Cerebrospinal fluid fills the ventricles and is produced by the choroid plexus.

12 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q6TUI4 DICER1 Endoribonuclease Dicer Bos taurus (Bovine) SS
Q25BN1 DICER1 Endoribonuclease Dicer Gallus gallus (Chicken) SS
Q9UPY3 DICER1 Endoribonuclease Dicer Homo sapiens (Human) EV
Q9BYX4 IFIH1 Interferon-induced helicase C domain-containing protein 1 Homo sapiens (Human) PR
Q96C10 DHX58 ATP-dependent RNA helicase DHX58 Homo sapiens (Human) PR
O95786 RIGI Antiviral innate immune response receptor RIG-I Homo sapiens (Human) EV
Q8R5F7 Ifih1 Interferon-induced helicase C domain-containing protein 1 Mus musculus (Mouse) PR
Q6Q899 Ddx58 Antiviral innate immune response receptor RIG-I Mus musculus (Mouse) SS
Q8R418 Dicer1 Endoribonuclease Dicer Mus musculus (Mouse) SS
Q6ATG6 Os05g0271300 Ribonuclease 3-like protein 2 Oryza sativa subsp japonica (Rice) PR
P34529 dcr-1 Endoribonuclease dcr-1 Caenorhabditis elegans PR
B3DLA6 dicer1 Endoribonuclease Dicer Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
10 20 30 40 50 60
MAGLQLVTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALEHNT IVCLNTGSGK
70 80 90 100 110 120
TFIAVLLIKE LSHQIRGENG KRTVFLVNAA SSVAQQASTV RTHSDLQVGD YMSEDMTSWP
130 140 150 160 170 180
EEMWNREMIE NQVLVMTCHI FLHVLKNGVL PLSKINLLVF DECHLAITGH PYREIMKICE
190 200 210 220 230 240
GCPSCPRILG LTASILNGKC DPCDLEEKIQ NLEKILQSNA ETATDLVVLD RYASQPREEV
250 260 270 280 290 300
LDCGQYQDQS GLSERLLNEL DEALNFLNDC NLSVHREDRD PTFISKQVLN DCRAVLTVLG
310 320 330 340 350 360
PWCADKAAGI MVRELQKYIK HEQEELNRKF LLFTDTILRK IHALCEEHFS PASLDLKFVT
370 380 390 400 410 420
PKVIRLLEIL HEYKPFERQQ FESVEWYNNR NQDNYVSWSD SEDDDEDEEA EAKEKTEANF
430 440 450 460 470 480
PSPFTNILCG IIFVERRYTA VVLNRLIKEA GKQDPELAYI SSNFITGHSI GKNQPRNKQM
490 500 510 520 530 540
EVEFRKQEEV LRKFRAHETN LLIATSIVEE GVDIPKCNLV VRFDLPTEYR SYVQSKGRAR
550 560 570 580 590 600
APVSNYIMLA DSERTKTFQE DLKTYKAIEK ILRNKCSKSA ECNDFELEPV TDDDNVLPPY
610 620 630 640 650 660
VLRSEDGGPR VTMNTAIGHV NRYCARLPSD PFTHLAPKCK TVEMNTGGYR STLFLPINSP
670 680 690 700 710 720
LRVPVTGPVM NCARLAEKAV ALLCCEKLHK IGELDDHLMP VGKETVKYEE ELDLHDEEET
730 740 750 760 770 780
SVPGRPGSTK RRQCSPKAIP ECLRGCYPVP EQPCYLYVIG MVLTTPLPDE LNFRRRKLYP
790 800 810 820 830 840
PEDTTRCFGI LTAKPIPRIP HFPVYTRSGE VTISIELQKS GFSLSAEQLE LITRLHQYIF
850 860 870 880 890 900
SHILRLEKPA LEFKPVEADS AYCVLPLNIV EDSNTLDLDF KFMEDIEKSE ARIGIPNTQY
910 920 930 940 950 960
TKQNPFIFKL EDYQDAVIIP RYRNFDQPHR FYVADVYTDL TPLSKFPSPE YETFAEYYKT
970 980 990 1000 1010 1020
KYNLDLSNVN QPLLDVDHTS SRLNLLTPRH LNQKGKALPL SSAEKRKAKW ESLQNKQILV
1030 1040 1050 1060 1070 1080
PELCAIHPIP ASLWRKAVCL PSILYRLHCL LTAEELRSQT AIDAGVGAQT LPPDFRYPNL
1090 1100 1110 1120 1130 1140
DFGWKKSIDS KSFISCPSAC MEEDDDHCKL GTSSDSNHTA PESCSMEVSQ PPEGAPNTPD
1150 1160 1170 1180 1190 1200
EKLETLTLPV TDLNKDCFPN LPNGTQADSD DLPHRSDVCQ CSQLGPLERD LSTQTTTSVS
1210 1220 1230 1240 1250 1260
VRPSPAGEPQ PWPSDECTGR SSDLCDPHVK KPTSKHCPKS ETATSTPAPS ETSSEDCRSA
1270 1280 1290 1300 1310 1320
CAGPAWDSPK TLGPNPGLIL QALTLSNASD GFNLERLEML GDSFLKHAIT TYLFCTYPDA
1330 1340 1350 1360 1370 1380
HEGRLSYMRS KKVSNCNLYR LGKKKGLPSR MVVSIFDPPV NWLPPGYVVN QDKSSTDKWD
1390 1400 1410 1420 1430 1440
SDENKDLANG KASDDEDEDD DDEPEEAEVE PSKEDVNVED DLEYYYEHIR FIDSMLIGSG
1450 1460 1470 1480 1490 1500
AFGKKISLQP TDPGYEWKAP KKAHNSHFSP DGGADEFDYS SWDAMCYLDP SKAGEEDDFV
1510 1520 1530 1540 1550 1560
VGFWNPSEEN CGTDIGKQSI SYDLHTEQCI ADKSIADCVE ALLGCYLTSC GERAAQLFLC
1570 1580 1590 1600 1610 1620
SLGLKVLPPE KQSSGGSAEL QYGWLKIPPR CMFEHPDAER TLNHLISGFL NFESKINYTF
1630 1640 1650 1660 1670 1680
KNKAYLLQAF THASYHYNTI TDCYQRLEFL GDAILDYLIT KHLYEDPRQH SPGVLTDLRS
1690 1700 1710 1720 1730 1740
ALVNNTIFAS LAVKYDYHKY FKAVSPELFH VIDDFVQFQL EKNEMQGMDS ELRRSEEDEE
1750 1760 1770 1780 1790 1800
KEEDIEVPKA MGDIFESLAG AIYMDSGMSL ETVWQVYYPM MRPLIEKFSA NVPRSPVREL
1810 1820 1830 1840 1850 1860
LEMEPETAKF SPAERTYDGK VRVTVEVVGK GKFKGVGRSY RIAKSAAARR ALRSLKANQP
QVQNN