Q6TUI4

SS
Gene name
DICER1 (DICER)
Protein name
Endoribonuclease Dicer
Names
EC 3.1.26.3
Species
Bos taurus (Bovine)
KEGG Pathway
bta:337871
EC number
3.1.26.3: Endoribonucleases producing 5'-phosphomonoesters
Protein Class

Descriptions

Dicer, a member of the Ribonuclease III family of enzymes, processes double-stranded RNA substrates into ~21-27 nucleotide products that trigger sequence-directed gene silencing by RNA interference (RNAi). The N-terminal domain of Dicer, which is homologous to DExD/H-box helicases, substantially attenuates the rate of substrate cleavage. Deletion or mutation of this domain activates human Dicer. Modest stimulation of catalysis by the full-length Dicer enzyme was observed in the presence of the TAR-RNA binding protein (TRBP), which physically interacts with the DExD/H-box domain. Thus, the DExD/H-box domain likely disrupts the functionality of the Dicer active site until a structural rearrangement occurs, perhaps upon assembly with its molecular partners

Autoinhibitory domains (AIDs)

1-604 (DExD/H-box helicase domain) SS

Target domain

1276-1404 (RNase IIIa domain);1667-1825 (RNase IIIb domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q6TUI4

Entry ID Method Resolution Chain Position Source
AF-Q6TUI4-F1 Predicted AlphaFoldDB

No variants for Q6TUI4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q6TUI4

No associated diseases with Q6TUI4

11 regional properties for Q6TUI4

Type Name Position InterPro Accession
domain Ribonuclease III domain 1284 - 1597 IPR000999-1
domain Ribonuclease III domain 1667 - 1848 IPR000999-2
domain Helicase, C-terminal domain-like 433 - 602 IPR001650
domain PAZ domain 890 - 1065 IPR003100
domain Dicer dimerisation domain 629 - 721 IPR005034
domain DEAD/DEAH box helicase domain 46 - 209 IPR011545
domain Helicase superfamily 1/2, ATP-binding domain 40 - 249 IPR014001
domain Double-stranded RNA-binding domain 1851 - 1915 IPR014720
domain Dicer, double-stranded RNA-binding domain 1831 - 1919 IPR044441
domain Dicer, platform domain 760 - 885 IPR048512
domain Dicer, partner-binding domain 268 - 366 IPR048513

Functions

Description
EC Number 3.1.26.3 Endoribonucleases producing 5'-phosphomonoesters
Subcellular Localization
  • Cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

5 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
RISC complex A ribonucleoprotein complex that contains members of the Argonaute family of proteins, small interfering RNAs (siRNAs) or microRNAs (miRNAs), and miRNA or siRNA-complementary mRNAs, in addition to a number of accessory factors. The RISC complex is involved in posttranscriptional repression of gene expression through downregulation of translation or induction of mRNA degradation.
RISC-loading complex A trimeric protein complex required for the formation of a mature RNA-induced silencing complex (RISC). In humans the complex is composed of the endonuclease Dicer (DICER1), TRBP (TARBP2) and the Argonaute protein Ago2 (EIF2C2/AGO2). Within the complex, Dicer and TRBP are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto Ago2. Ago2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from Dicer and TRBP. This complex has endoribonuclease activity.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
deoxyribonuclease I activity Catalysis of the endonucleolytic cleavage of DNA to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end products.
helicase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
ribonuclease III activity Catalysis of the endonucleolytic cleavage of RNA with 5'-phosphomonoesters and 3'-OH termini; makes two staggered cuts in both strands of dsRNA, leaving a 3' overhang of 2 nt.
RNA binding Binding to an RNA molecule or a portion thereof.

4 GO annotations of biological process

Name Definition
apoptotic DNA fragmentation The cleavage of DNA during apoptosis, which usually occurs in two stages
global gene silencing by mRNA cleavage A posttranscriptional gene silencing pathway that involves the cleavage of mRNAs in a non-gene-specific manner.
pre-miRNA processing A process involved in the conversion of a pre-microRNA transcript into a mature microRNA molecule.
siRNA processing A process leading to the generation of a functional small interfering RNA (siRNA). Includes the cleavage of double-stranded RNA to form small interfering RNA molecules (siRNAs) of 21-23 nucleotides. May also include amplification of the siRNA by RNA-directed RNA polymerase.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q25BN1 DICER1 Endoribonuclease Dicer Gallus gallus (Chicken) SS
Q9UPY3 DICER1 Endoribonuclease Dicer Homo sapiens (Human) EV
Q8R418 Dicer1 Endoribonuclease Dicer Mus musculus (Mouse) SS
Q6ATG6 Os05g0271300 Ribonuclease 3-like protein 2 Oryza sativa subsp japonica (Rice) PR
P34529 dcr-1 Endoribonuclease dcr-1 Caenorhabditis elegans PR
B3DLA6 dicer1 Endoribonuclease Dicer Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) SS
Q6TV19 dicer1 Endoribonuclease Dicer Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MKSPALQPLS MAGLQLMTPA SSPMGPFFGL PWQQEAIHDN IYTPRKYQVE LLEAALDHNT
70 80 90 100 110 120
IVCLNTGSGK TFIAVLLTKE LSYQIRGDFN RNGKRTVFLV NSANQVAQQV SAVRTHSDLK
130 140 150 160 170 180
VGEYSNLEVS ASWTKEKWNQ EFTKHQVLIM TCYVALNVLK NGYLSLSDIN LLVFDECHLA
190 200 210 220 230 240
ILDHPYREIM KLCENCPSCP RILGLTASIL NGKCDPEELE EKIQKLEKIL KSNAETATDL
250 260 270 280 290 300
VVLDRYTSQP CEIVVDCGPF TDRSGLYERL LMELEEALNF INDCNISVHS KERDSTLISK
310 320 330 340 350 360
QILSDCRAVL VVLGPWCADK VAGMMVRELQ KHIKHEQEEL HRKFLLFTDT FLRKIHALCE
370 380 390 400 410 420
EHFSPASLDL KFVTPKVIKL LEILRKYKPY ERQQFESVEW YNNRNQDNYV SWSDSEDDEE
430 440 450 460 470 480
DEEIEEKEKP ETNFPSPFTN ILCGIIFVER RYTAVVLNRL IKEAGKQDPE LAYISSNFIT
490 500 510 520 530 540
GHGIGKNQPR NKQMEAEFRK QEEVLRKFRA HETNLLIATS IVEEGVDIPK CNLVVRFDLP
550 560 570 580 590 600
TEYRSYVQSK GRARAPISNY VMLADTDKIK SFEEDLKTYK AIEKILRNKC SKSVDTGEAD
610 620 630 640 650 660
TEPVVDDDDV FPPYVLRPED GPRVTINTAI GHVNRYCARL PSDPFTHLAP KCRTRELPDG
670 680 690 700 710 720
TFYSTLYLPI NSPLRASIVG PPMSCIRLAE RVVALICCEK LHKIGELDDH LMPVGKETVK
730 740 750 760 770 780
YEEELDLHDE EETSVPGRPG STKRRQCYPK AIPECLRESY PRPGQPCYLY VIGMVLTTPL
790 800 810 820 830 840
PDELNFRRRK LYPPEDTTRC FGILTAKPIP QIPHFPVYTR SGEVTISIEL KKSGFTLSLQ
850 860 870 880 890 900
MLELITRLHQ YIFSHILRLE KPALEFKPTD ADSAYCVLPL NVVNDSSTLD IDFKFMEDIE
910 920 930 940 950 960
KSEARIGIPS TKYSKETPFV FKLEDYQDAV IIPRYRNFDQ PHRFYVADVY TDLTPLSKFP
970 980 990 1000 1010 1020
SPEYETFAEY YKTKYNLDLT NLNQPLLDVD HTSSRLNLLT PRHLNQKGKA LPLSSAEKRK
1030 1040 1050 1060 1070 1080
AKWESLQNKQ ILVPELCAIH PIPASLWRKA VCLPSILYRL HCLLTAEELR AQTASDAGVG
1090 1100 1110 1120 1130 1140
VRSLPVDFRY PNLDFGWKKS IDSKSFISIA NSSSAENENY CKHSTIVVPE NAAHQGANRT
1150 1160 1170 1180 1190 1200
SPLENHDQMS VNCRTLFSES PGKLQIEVST DLTAINGLSY NKSLANGSYD LANRDFCQGN
1210 1220 1230 1240 1250 1260
HLNYYKQEIP VQPTTSYPIQ NLYNYENQPK PSDECTLLSN KYLDGNANTS TSDGSPVTAA
1270 1280 1290 1300 1310 1320
VPGTTETGEA PPDRTASEQS PSPGYSSRTL GPNPGLILQA LTLSNASDGF NLERLEMLGD
1330 1340 1350 1360 1370 1380
SFLKHAITTV SLSALILDAH EGRLSYMRSK KVSNCNLYRL GKKKGLPSRM VVSIFDPPVN
1390 1400 1410 1420 1430 1440
GLPPGYVVNQ DKSNTEKWEK DEMTKDCMLA NGKLDDDFEE EEEEEEDLMW RAHKEDADDE
1450 1460 1470 1480 1490 1500
DDFLEYDQEH IKFIDNMLMG SGAFVKKISL SPFSATDSAY EWKMPKKSSL GSLPFSSDFE
1510 1520 1530 1540 1550 1560
DFDYSSWDAM CYLDPSKAVE EDDFVVGFWN PSEENCGVDT GKQSISYDLH TEQCIADKSI
1570 1580 1590 1600 1610 1620
ADCVEALLGC YLTSCGERAA QLFLCSLGLK VLPVIKRTDR EKAMCPTREN FTSQQKNLSG
1630 1640 1650 1660 1670 1680
SRAAASGAGY RASVLKDLEY GCLKIPPRCM FDHPEADRTL RHLISGFENF EKKINYRFKN
1690 1700 1710 1720 1730 1740
KAYLLQAFTH ASYHYNTITD CYQRLEFLGD AILDYLITKH LYEDPRQHSP GVLTDLRSAL
1750 1760 1770 1780 1790 1800
VNNTIFASLA VKYDYHKYFK AVSPELFHVI DDFVQFQLEK NEMQGMDSEL RRSEEDEEKE
1810 1820 1830 1840 1850 1860
EDIEVPKAMG DIFESLAGAI YMDSGMSLET VWQVYYPMMR PLIEKFSANV PRSPVRELLE
1870 1880 1890 1900 1910 1920
MEPEITKFSP AERTYDGKVR VTVEVVGKGK FKGVGRSYRI AKSAAARRAL RSLKANQPQV
PNS