Q6RT24

SS
Gene name
Cenpe
Protein name
Centromere-associated protein E
Names
Centromere protein E , CENP-E , Kinesin superfamily protein 10 , KIF10 , Motor domain of KIF10
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:229841
EC number
Protein Class

Descriptions

Autoinhibition of the Kinesin-7 family member CENPE involves direct interactions of the C-terminal tail domains with their motor domains. The autoinhibition can be relieved by phosphorylation of the inhibitory tail domain by the kinases monopolar spindle protein 1 (MPS1) and cyclin-dependent kinase 1 (CDK1)-cyclin B, resulting in processive motility on microtubules.

Autoinhibitory domains (AIDs)

2168-2474 (C-terminal tail) SS

Target domain

4-337 (Kinesin motor domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q6RT24

Entry ID Method Resolution Chain Position Source
AF-Q6RT24-F1 Predicted AlphaFoldDB

No variants for Q6RT24

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q6RT24

No associated diseases with Q6RT24

3 regional properties for Q6RT24

Type Name Position InterPro Accession
conserved_site 14-3-3 protein, conserved site 44 - 54 IPR023409-1
conserved_site 14-3-3 protein, conserved site 217 - 236 IPR023409-2
domain 14-3-3 domain 5 - 248 IPR023410

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton, spindle
  • Chromosome, centromere, kinetochore
  • Chromosome, centromere
  • Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division (PubMed:12361599, PubMed:12925705)
  • Recruited to the kinetochore in a SEPT7, CENPQ and TRAPPC12-dependent manner
  • Recruited to the pericentromeric/centromeric regions of the chromosome in a CTCF-dependent manner (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
chromosome, centromeric region The region of a chromosome that includes the centromeric DNA and associated proteins. In monocentric chromosomes, this region corresponds to a single area of the chromosome, whereas in holocentric chromosomes, it is evenly distributed along the chromosome.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
outer kinetochore The region of a kinetochore most external to centromeric DNA; this outer region mediates kinetochore-microtubule interactions.
spindle The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
kinetochore binding Binding to a kinetochore, a proteinaceous structure on a condensed chromosome, beside the centromere, to which the spindle fibers are attached.
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule motor activity A motor activity that generates movement along a microtubule, driven by ATP hydrolysis.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.

18 GO annotations of biological process

Name Definition
attachment of mitotic spindle microtubules to kinetochore The cell cycle process in which spindle microtubules become physically associated with the proteins making up the kinetochore complex as part of mitotic metaphase plate congression.
attachment of spindle microtubules to kinetochore The process in which spindle microtubules become physically associated with the proteins making up the kinetochore complex.
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
establishment of protein localization The directed movement of a protein to a specific location.
lateral attachment of mitotic spindle microtubules to kinetochore The cell cycle process in which sister chromatids become laterally attached to spindle microtubules as part of mitotic metaphase plate congression. Attachment precedes migration along microtubules towards the spindle equator (metaphase plate).
metaphase chromosome alignment The alignment of chromosomes at the metaphase plate (spindle equator), a plane halfway between the poles of the spindle.
microtubule-based movement A microtubule-based process that results in the movement of organelles, other microtubules, or other cellular components. Examples include motor-driven movement along microtubules and movement driven by polymerization or depolymerization of microtubules.
mitotic cell cycle Progression through the phases of the mitotic cell cycle, the most common eukaryotic cell cycle, which canonically comprises four successive phases called G1, S, G2, and M and includes replication of the genome and the subsequent segregation of chromosomes into daughter cells. In some variant cell cycles nuclear replication or nuclear division may not be followed by cell division, or G1 and G2 phases may be absent.
mitotic metaphase chromosome alignment The cell cycle process in which chromosomes are aligned at the metaphase plate, a plane halfway between the poles of the mitotic spindle, during mitosis.
mitotic spindle assembly checkpoint signaling A mitotic cell cycle checkpoint that delays mitotic sister chromatid separation and consequently the mitotic metaphase/anaphase transition until the spindle is correctly assembled and chromosomes are attached to the spindle. Spindle assembly checkpoint signalling begins with the activated Mph family kinase, and results in the inhibition of the Anaphase Promoting Complex and its activator Sleepy/Cdc20 by the mitotic checkpoint complex (MCC).
positive regulation of attachment of spindle microtubules to kinetochore Any process that activates or increases the frequency, rate or extent of the attachment of spindle microtubules to the kinetochore.
positive regulation of chromosome segregation Any process that activates or increases the frequency, rate or extent of chromosome segregation, the process in which genetic material, in the form of chromosomes, is organized and then physically separated and apportioned to two or more sets.
positive regulation of mitotic metaphase/anaphase transition Any process that activates or increases the frequency, rate or extent of the cell cycle process in which a cell progresses from metaphase to anaphase during mitosis, triggered by the activation of the anaphase promoting complex by Cdc20/Sleepy homolog which results in the degradation of Securin.
positive regulation of protein kinase activity Any process that activates or increases the frequency, rate or extent of protein kinase activity.
regulation of developmental process Any process that modulates the frequency, rate or extent of development, the biological process whose specific outcome is the progression of a multicellular organism over time from an initial condition (e.g. a zygote, or a young adult) to a later condition (e.g. a multicellular animal or an aged adult).
regulation of mitotic nuclear division Any process that modulates the frequency, rate or extent of mitosis.
regulation of protein stability Any process that affects the structure and integrity of a protein, altering the likelihood of its degradation or aggregation.
spindle assembly involved in female meiosis I The aggregation, arrangement and bonding together of a set of components to form the spindle during meiosis I of a meiotic cell cycle in females. An example of this is found in Drosophila melanogaster.

1 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q02224 CENPE Centromere-associated protein E Homo sapiens (Human) EV
10 20 30 40 50 60
MAEEASVAVC VRVRPLNSRE EELGEATHIY WKTDKNAIYQ SDGGKSFQFD RVFDSNETTK
70 80 90 100 110 120
NVYEEIAVPI ISSAIQGYNG TIFAYGQTAS GKTHTMMGSE DCLGVIPRAI HDIFQRIKKF
130 140 150 160 170 180
PEREFLLRVS YMEIYNETIT DLLCNAQKMK PLIIREDTNR TVYVSDLTEE VVYTAEMALK
190 200 210 220 230 240
WLATGEKNRH YGITKMNQRS SRSHTIFRMI LESREKAEPS NCDGSVKVSH LNLVDLAGSE
250 260 270 280 290 300
RAAQTGAEGV RLKEGCFINR NLFILGQVIK KLSDGQVGGF INYRDSKLTR ILQNSLGGNA
310 320 330 340 350 360
KTRIICTITP ASLDETLTTL QFASTAKYMK NTPYVNEVSN DEALLKRYRR EIADLRKQLE
370 380 390 400 410 420
EVNTKTRAQE MEKDQLAQLL DEKDLLQKVQ DEKINNLKRM LVTSSSIALQ QELEIKRKRR
430 440 450 460 470 480
VTWCYGKMKD SNYEKEFKVP TSITTRKRKT SVTSLRENSL MKFGESAASS EFEMLNNTLE
490 500 510 520 530 540
SLAEVEWSSA TTLLSEENVE SELNSLNAQY NDLVLDYEQL RRENEDLKLK LKEKNELEEF
550 560 570 580 590 600
ELLEQKEERD QEMQLMHEVS NLKNLIKHAE EYNQDLENDL SSKVKLLKEK EEQIKNLQEY
610 620 630 640 650 660
IDAQKSEKMK IDLSYTSDAT EDLKQAMRTL SDLDTVALDA KKESAFLRSE NLELKEKINE
670 680 690 700 710 720
LSDSRKQMES DIQMYQRQLE AKKKMQTDLD KELQLAFQEI SKLSALVDGK GLLSNLELEK
730 740 750 760 770 780
RITDLQKELN KEAEEKQTLQ EEVNLLSELK SLPSEVETLR RELYEKSEEL HIITTEREKL
790 800 810 820 830 840
FSEMAHKDSR IQGLLEEIGN TRDDLATSQL SRRGSDGEWQ ALESLHAELE HRHAGVLEER
850 860 870 880 890 900
ERLKQEIGAL SKEAESLAFS LDSVKAELSH KTQELEQKTV EGQERLNKME ALREELESRD
910 920 930 940 950 960
SSLQSVEKEK VLLTEKLQQA LKEVKALTQE KKNLKQLQES LQTERDQLRS DIQDTVNMNI
970 980 990 1000 1010 1020
DTQEQLLNAL ESLKQHQETI NMLKMKAAEE LSDNLHVKDR GGARDEAQQK MDGIDEQNES
1030 1040 1050 1060 1070 1080
AHTLLGGGKD NEVTEEQRKI DSLMQENSGL QQTLESVRAE KEQLKMDLKE NIEMSIENQE
1090 1100 1110 1120 1130 1140
ELRILRDELK RQQEVAAQEK DHATEKTQEL SRTQERLAKT EEKLEEKNQK LQETQQQLLS
1150 1160 1170 1180 1190 1200
TQEAMSKLQA KVIDMESLQN EFRNQGLALE RVETEKLELA QRLHESYEEV KSITKERNDL
1210 1220 1230 1240 1250 1260
KELQESFEIE KKQLKEYARE IEAEGLQAKE ELNIAHANLK EYQEIITELR GSISENEAQG
1270 1280 1290 1300 1310 1320
ASTQDTAKSA PELQGEVPEL LEQELLPVVK EARHSAEKVN GLEPVGAHSR TVHSMTMEGI
1330 1340 1350 1360 1370 1380
EIGNLRLTKK LEESQMEISC LTREREDLRR TQETLQVECT QLKEDARRTL ANHLETEEEL
1390 1400 1410 1420 1430 1440
NLARCCLKEQ ENKIDTLITS LSQRETELSS VRGQLALTTA ELERKVQELC EKQEELTRKE
1450 1460 1470 1480 1490 1500
TSEAQGKMSE LEQLRELLLA QASALQNAES DRLRLNTQLE ESQEEMKTLR EEREELRRMQ
1510 1520 1530 1540 1550 1560
EALHVESEQQ KESMKEISSK LQELQNKEYE CLAMKTINET QGSRCEMDHL NQQLEAQKST
1570 1580 1590 1600 1610 1620
LEKVEMENVN LTQRLHETLE EMRSVAKERD ELWSMEERLT VERDQLKKSL EETVTKGMEK
1630 1640 1650 1660 1670 1680
EEELRVAHVH LEEHQETINK LRKMVSDYTD EISHTQGDLK HTNAVVEAQN QDLREKEHQL
1690 1700 1710 1720 1730 1740
SQVKADLRET VDQMEQLKKK LEAQSSTLES REIEKLELTQ QLNENLKKIT LVTKENDSLK
1750 1760 1770 1780 1790 1800
IMDEALREER DQLRKSLQQT EARDLENQEK LRIAHMNLKE HQETIDRLME TMSEKTEEIS
1810 1820 1830 1840 1850 1860
NMKMELENVN MKLQEKVQEL KTSERQRVKL KADASEAKKE LKEQGLTLSK IEMENLNLAQ
1870 1880 1890 1900 1910 1920
KIHENLEEMK SVRKERDDLK KLEEILRMER DQLKDNLREA MLKAHQNHEE TMKCGKGLLC
1930 1940 1950 1960 1970 1980
AGEYCTGRLR EKCFRIEKLL KRYSEMANDY ECLNKVSLDL ERETKTQKEL SVTVRTKLSL
1990 2000 2010 2020 2030 2040
PHTQTKEMEK LLTANQRCSL EFHRALKRLK YVLSSIARIK EEQHESINKR EMAFIQEVEK
2050 2060 2070 2080 2090 2100
QNELQIQIQS LSQTYRIPAR DLQIKLSQEM DLHIEEMLKD FSENDFLTIK TEVQQVLNNR
2110 2120 2130 2140 2150 2160
KEITEFLGKW LNTLFDTENL KSTIQKENKS IGLVNNFYHS RITAMINEST EFEERSATRS
2170 2180 2190 2200 2210 2220
KDLDQYLKSL KETTEQLSEV YQTLTASQSV VHLHPTVQPS TRDSERPQAA SGAEQLTSKN
2230 2240 2250 2260 2270 2280
KIALGAVPYK EEIEDLKMQL VKSDLEKKAT AKEFDKKILS LKATVEHQEE MIRLLRENLR
2290 2300 2310 2320 2330 2340
GHQQAQDTSM ISEQDSQLLS KPLTCGGGSG IVQSTKALIL KSEYKRMGSE ISKLKQQNEQ
2350 2360 2370 2380 2390 2400
LRKQNNQLLS DNSQLSNEVK TWEERTLKRD SYRETTCENS PKSPKVTRTD SKRRQNTTSQ
2410 2420 2430 2440 2450 2460
CRAQNLQDPV PKDSPKSWFF DNRSKSLPAP HPIRYFDNSS LGLCPEPDDV ENVEPKTDLC
2470
QASLEKDVSQ CKTQ