Q6NYW6

SS
Gene name
clasp2
Protein name
CLIP-associating protein 2
Names
Cytoplasmic linker-associated protein 2
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
KEGG Pathway
dre:404604
EC number
Protein Class

Descriptions

CLASPs, major microtubule-stabilizing factors in interphase and mitosis, prevent microtubule from switching form growth to shortening by stabilizing growing microtubule ends, and thus suppress microtubule catastrophes. CLASP2a consists of three TOG-like domains (termed TOG1, 2, and 3) and a C-terminal domain, CLIP-interacting domain (CLIP-ID) responsible for interaction with CLIP-170 and other partners. CLASP2a TOG2 is necessary and sufficient for catastrophe inhibition. The inhibition function is suppressed by the C-terminal domain CLIP-ID while the TOG1 domain or the CLIP-ID partner binding to CLIP-ID can release the autoinhibition.

Autoinhibitory domains (AIDs)

1170-1279 (C-terminal domain CLIP-ID) SS

Target domain

636-893 (TOG2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q6NYW6

Entry ID Method Resolution Chain Position Source
AF-Q6NYW6-F1 Predicted AlphaFoldDB

No variants for Q6NYW6

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q6NYW6

6 associated diseases with Q6NYW6

[MIM: 600880]: Budd-Chiari syndrome (BDCHS)

A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. {ECO:0000269|PubMed:16707754}. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry.

[MIM: 263300]: Polycythemia vera (PV)

A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly. {ECO:0000269|PubMed:15781101, ECO:0000269|PubMed:15793561, ECO:0000269|PubMed:15858187, ECO:0000269|PubMed:16603627}. Note=The disease is caused by variants affecting the gene represented in this entry.

[MIM: 614521]: Thrombocythemia 3 (THCYT3)

A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes. {ECO:0000269|PubMed:16325696, ECO:0000269|PubMed:22397670}. Note=The disease may be caused by variants affecting the gene represented in this entry.

[MIM: 254450]: Myelofibrosis (MYELOF)

A disorder characterized by replacement of the bone marrow by fibrous tissue, occurring in association with a myeloproliferative disorder. Clinical manifestations may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension. Note=The disease is caused by variants affecting the gene represented in this entry.

[MIM: 601626]: Leukemia, acute myelogenous (AML)

A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. {ECO:0000269|PubMed:16247455}. Note=The disease is caused by variants affecting the gene represented in this entry.

Without disease ID
  • A syndrome caused by obstruction of hepatic venous outflow involving either the hepatic veins or the terminal segment of the inferior vena cava. Obstructions are generally caused by thrombosis and lead to hepatic congestion and ischemic necrosis. Clinical manifestations observed in the majority of patients include hepatomegaly, right upper quadrant pain and abdominal ascites. Budd-Chiari syndrome is associated with a combination of disease states including primary myeloproliferative syndromes and thrombophilia due to factor V Leiden, protein C deficiency and antithrombin III deficiency. Budd-Chiari syndrome is a rare but typical complication in patients with polycythemia vera. {ECO:0000269|PubMed:16707754}. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry.
  • A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly. {ECO:0000269|PubMed:15781101, ECO:0000269|PubMed:15793561, ECO:0000269|PubMed:15858187, ECO:0000269|PubMed:16603627}. Note=The disease is caused by variants affecting the gene represented in this entry.
  • A myeloproliferative disorder characterized by excessive platelet production, resulting in increased numbers of circulating platelets. It can be associated with spontaneous hemorrhages and thrombotic episodes. {ECO:0000269|PubMed:16325696, ECO:0000269|PubMed:22397670}. Note=The disease may be caused by variants affecting the gene represented in this entry.
  • A disorder characterized by replacement of the bone marrow by fibrous tissue, occurring in association with a myeloproliferative disorder. Clinical manifestations may include anemia, pallor, splenomegaly, hypermetabolic state, petechiae, ecchymosis, bleeding, lymphadenopathy, hepatomegaly, portal hypertension. Note=The disease is caused by variants affecting the gene represented in this entry.
  • A subtype of acute leukemia, a cancer of the white blood cells. AML is a malignant disease of bone marrow characterized by maturational arrest of hematopoietic precursors at an early stage of development. Clonal expansion of myeloid blasts occurs in bone marrow, blood, and other tissue. Myelogenous leukemias develop from changes in cells that normally produce neutrophils, basophils, eosinophils and monocytes. {ECO:0000269|PubMed:16247455}. Note=The disease is caused by variants affecting the gene represented in this entry.

19 regional properties for Q6NYW6

Type Name Position InterPro Accession
domain FERM domain 37 - 380 IPR000299
domain Protein kinase domain 545 - 809 IPR000719-1
domain Protein kinase domain 849 - 1126 IPR000719-2
domain SH2 domain 397 - 487 IPR000980
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 546 - 805 IPR001245-1
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 849 - 1120 IPR001245-2
active_site Tyrosine-protein kinase, active site 972 - 984 IPR008266
binding_site Protein kinase, ATP binding site 855 - 883 IPR017441
domain FERM central domain 152 - 261 IPR019748
domain Band 4.1 domain 33 - 270 IPR019749
domain Tyrosine-protein kinase, catalytic domain 545 - 805 IPR020635-1
domain Tyrosine-protein kinase, catalytic domain 849 - 1123 IPR020635-2
domain Janus kinase 2, pseudokinase domain 545 - 806 IPR035588
domain Janus kinase 2, catalytic domain 844 - 1127 IPR035589
domain Tyrosine-protein kinase JAK2, SH2 domain 386 - 482 IPR035860
domain JAK2, FERM domain C-lobe 266 - 386 IPR037838
domain JAK, FERM F2 lobe domain 143 - 261 IPR041046
domain FERM F1 lobe ubiquitin-like domain 39 - 134 IPR041155
domain JAK1-3/TYK2, pleckstrin homology-like domain 302 - 381 IPR041381

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm, cytoskeleton
  • Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
  • Chromosome, centromere, kinetochore
  • Cytoplasm, cytoskeleton, spindle
  • Golgi apparatus
  • Golgi apparatus, trans-Golgi network
  • Cell membrane
  • Cell projection, ruffle membrane
  • Localizes to microtubule plus ends
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

12 GO annotations of cellular component

Name Definition
basal cortex The region that lies just beneath the plasma membrane on the basal edge of a cell.
cell leading edge The area of a motile cell closest to the direction of movement.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasmic microtubule Any microtubule in the cytoplasm of a cell.
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
kinetochore A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.
microtubule Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle.
microtubule organizing center An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides.
mitotic spindle A spindle that forms as part of mitosis. Mitotic and meiotic spindles contain distinctive complements of proteins associated with microtubules.
ruffle membrane The portion of the plasma membrane surrounding a ruffle.
spindle microtubule Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.
trans-Golgi network The network of interconnected tubular and cisternal structures located within the Golgi apparatus on the side distal to the endoplasmic reticulum, from which secretory vesicles emerge. The trans-Golgi network is important in the later stages of protein secretion where it is thought to play a key role in the sorting and targeting of secreted proteins to the correct destination.

2 GO annotations of molecular function

Name Definition
microtubule binding Binding to a microtubule, a filament composed of tubulin monomers.
microtubule plus-end binding Binding to the plus end of a microtubule.

13 GO annotations of biological process

Name Definition
cell division The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells.
establishment of mitotic spindle localization The cell cycle process in which the directed movement of the mitotic spindle to a specific location in the cell occurs.
exit from mitosis The cell cycle transition where a cell leaves M phase and enters a new G1 phase. M phase is the part of the mitotic cell cycle during which mitosis and cytokinesis take place.
Golgi organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the Golgi apparatus.
hematopoietic stem cell homeostasis Any biological process involved in the maintenance of the steady-state number of hematopoietic stem cells within a population of cells.
microtubule anchoring Any process in which a microtubule is maintained in a specific location in a cell.
microtubule cytoskeleton organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising microtubules and their associated proteins.
microtubule nucleation The process in which tubulin alpha-beta heterodimers begin aggregation to form an oligomeric tubulin structure (a microtubule seed). Microtubule nucleation is the initiating step in the formation of a microtubule in the absence of any existing microtubules ('de novo' microtubule formation).
microtubule organizing center organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a microtubule organizing center, a structure from which microtubules grow.
mitotic spindle assembly Mitotic bipolar spindle assembly begins with spindle microtubule nucleation from the separated spindle pole body, includes spindle elongation during prometaphase, and is complete when all kinetochores are stably attached the spindle, and the spindle assembly checkpoint is satisfied.
mitotic spindle organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the microtubule spindle during a mitotic cell cycle.
regulation of microtubule polymerization or depolymerization Any process that modulates the frequency, rate or extent of microtubule polymerization or depolymerization by the addition or removal of tubulin heterodimers from a microtubule.
regulation of microtubule-based process Any process that modulates the frequency, rate or extent of any cellular process that depends upon or alters the microtubule cytoskeleton.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P38198 STU1 Protein STU1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q9NBD7 chb CLIP-associating protein Drosophila melanogaster (Fruit fly) SS
Q7Z460 CLASP1 CLIP-associating protein 1 Homo sapiens (Human) SS
O75122 CLASP2 CLIP-associating protein 2 Homo sapiens (Human) EV
Q80TV8 Clasp1 CLIP-associating protein 1 Mus musculus (Mouse) SS
Q8BRT1 Clasp2 CLIP-associating protein 2 Mus musculus (Mouse) SS
Q99JD4 Clasp2 CLIP-associating protein 2 Rattus norvegicus (Rat) SS
10 20 30 40 50 60
MALSLSQDRS FDDDDSVDGS RPSSAQAAFK VPKVPKKPAE SASSSRRPSA TGAAKSGASK
70 80 90 100 110 120
EGAGAVDEED FIKAFTDVPT VQIYSTRDLE DNLNKIREVL SDDKHDWDHR ANALKKIRSL
130 140 150 160 170 180
LVAGATDYDC FYQHLRLLDG AFKLSAKDLR SQVVREACIT VAYLSTLLGN KFDHGAEGIV
190 200 210 220 230 240
PVLFNLIPNC AKVMATSGTA AIRIIIRHTH VPRLIPLIAS NCTSKSVAVR RRCYEFLDLL
250 260 270 280 290 300
LQEWQTHSLE RHAAVLVESI KKGIRDADAE ARVEARKAYW GLRAHFPGEA ESLYNSLESS
310 320 330 340 350 360
YQRTLQSCLK SSGSVASLPQ SDRSSSSSQE SLNRPLSKWS AAPGRVPAGS KSSGSPASLQ
370 380 390 400 410 420
RSRSDVDVNA AAGAKARHSG QAGGAGRVTT GLTPGSYASL GRLRTKQTLS TASSVGSSQV
430 440 450 460 470 480
DSRGRTRSKM ASQSQRSDDS DCTPGSQSAT PVGAGSRSGS PGRVLASTAL STLSTGAQRV
490 500 510 520 530 540
SAAPGSHRRS RIPRSQGCSR DSSPTRLSVA PSNISHIYNG SKGARGSRIP RPSVSQGCSR
550 560 570 580 590 600
EASRESSRDT SPVRSFTPLG SGLGMSQSSR LSSSVSAMRV LNTGSDVEEA LADALKKPAR
610 620 630 640 650 660
RRYDTYGMYS DDDANSDASS ACSERSYSSR NGSIPTYMRQ TEDVAEVLNR CASANWSERK
670 680 690 700 710 720
EGLMGLQALL KNHRTLSRVE LKRLCEIFTR MFADPHSKRD PRGFGTVESG ISSASFKRVF
730 740 750 760 770 780
SMFLETLVDF IAVHKEDLQD WLFVLLTQLL KKMGADLLGS VQAKVQKALD VTRESFPNDL
790 800 810 820 830 840
QFTILMRFTV DQTQTPNLKV KVAILKYIET LTLQMEPQDF VNTGETRLAV SRIITWTTEP
850 860 870 880 890 900
KSSDVRKAAQ SVLISLFQLN TPEFTMLLGA LPKTFQDGAT KLLQNHLRNT GNTAQASIGS
910 920 930 940 950 960
PLTRHTPRSP ASWSSPLTSP TNTSQNTPSP SAFDYDTENM NSEEIYSSLR GVSQAIQNFS
970 980 990 1000 1010 1020
VRSQEDMTEP PRKREGDGGE ETVDSGRTAL DNKTSLLNTM PLLSSSPRPS RDYQPVNYSD
1030 1040 1050 1060 1070 1080
SSFGSSSFNK SLKDADQEES LTDDSGVDQS EVVAELLKEL SNHSERVEER KAALCELMRL
1090 1100 1110 1120 1130 1140
IRETQLHVWD EHFKTILLLL LETLGDGEHV IRALALRVLK EILNRQPWRF KNYAELTIMK
1150 1160 1170 1180 1190 1200
ALEAHKDPHK EVVRAAEEAA SMLATSISPD QCIKVLCPII QSADYPINLA AIKMLTKVID
1210 1220 1230 1240 1250 1260
RLPKEGLIQM LPEIVPGLIQ GYDNSESSVR KACVFCLVAI YAVIGEDLKP HLSQLSGSKL
1270 1280
KLLNLYIKRA QSGSSGSDQS SDVGGQGL