Q69ZK0
SS
Gene name |
Prex1 (Kiaa1415) |
Protein name |
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein |
Names |
P-Rex1 , PtdIns, 3,4,5-dependent Rac exchanger 1 |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:277360 |
EC number |
|
Protein Class |
|
Descriptions
Phosphatidylinositol (3,4,5)-trisphosphate (PIP3)-dependent Rac exchanger 1 (P-Rex1) catalyzes the exchange of GDP for GTP on Rac GTPases, thereby triggering changes in the actin cytoskeleton and in transcription. P-Rex1 contains a DH domain, a PH domain, two DEP domains, and two PDZ domains. The DEP1 domain alone can autoinhibit the activity of P-Rex1 by interacting with the DH/PH domains.
Autoinhibitory domains (AIDs)
Target domain |
33-403 (DH/PH domains) |
Relief mechanism |
Ligand binding, Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Ravala SK et al. (2020) "The first DEP domain of the RhoGEF P-Rex1 autoinhibits activity and contributes to membrane binding", The Journal of biological chemistry, 295, 12635-12647
- Chang YG et al. (2022) "Structure of the metastatic factor P-Rex1 reveals a two-layered autoinhibitory mechanism", Nature structural & molecular biology, 29, 767-773
Autoinhibited structure
Activated structure
1 structures for Q69ZK0
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q69ZK0-F1 | Predicted | AlphaFoldDB |
76 variants for Q69ZK0
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs233395411 | 7 | G>S | No | EVA | |
| rs3392568512 | 135 | D>G | No | EVA | |
| rs3388610676 | 150 | A>V | No | EVA | |
| rs3388599058 | 152 | R>C | No | EVA | |
| rs3388611714 | 218 | V>L | No | EVA | |
| rs3388605655 | 240 | E>G | No | EVA | |
| rs3388605655 | 240 | E>V | No | EVA | |
| rs3388614441 | 241 | K>SDG* | No | EVA | |
| rs3388610630 | 254 | G>D | No | EVA | |
| rs3388605663 | 278 | A>V | No | EVA | |
| rs3388615478 | 287 | F>Y | No | EVA | |
| rs3388609987 | 305 | S>C | No | EVA | |
| rs3388611704 | 316 | N>D | No | EVA | |
| rs3388610674 | 409 | E>V | No | EVA | |
| rs3388615563 | 423 | I>N | No | EVA | |
| rs3507696647 | 433 | V>I | No | EVA | |
| rs3388614572 | 468 | N>I | No | EVA | |
| rs3411762468 | 469 | G>D | No | EVA | |
| rs3388615489 | 472 | H>Y | No | EVA | |
| rs3388615614 | 476 | D>N | No | EVA | |
| rs3388614455 | 506 | M>K | No | EVA | |
| rs3388606771 | 521 | A>T | No | EVA | |
| rs3388599028 | 538 | V>F | No | EVA | |
| rs3388610697 | 546 | W>R | No | EVA | |
| rs3388608914 | 550 | Q>* | No | EVA | |
| rs3392354330 | 555 | T>K | No | EVA | |
| rs3388615481 | 560 | V>M | No | EVA | |
| rs3388614583 | 570 | G>S | No | EVA | |
| rs3388613547 | 601 | S>N | No | EVA | |
| rs3388615462 | 603 | N>K | No | EVA | |
| rs3540879257 | 624 | P>L | No | EVA | |
| rs3388615564 | 653 | E>D | No | EVA | |
| rs262018289 | 686 | F>S | No | EVA | |
| rs3388608897 | 716 | F>S | No | EVA | |
| rs3388608897 | 716 | F>Y | No | EVA | |
| rs3388615636 | 726 | V>F | No | EVA | |
| rs3388616271 | 729 | V>A | No | EVA | |
| rs3388613576 | 730 | G>S | No | EVA | |
| rs3388611683 | 780 | Y>C | No | EVA | |
| rs3388613580 | 781 | Q>P | No | EVA | |
| rs3388605622 | 782 | W>* | No | EVA | |
| rs3392213568 | 814 | L>Q | No | EVA | |
| rs3388610027 | 815 | P>S | No | EVA | |
| rs3388609973 | 840 | H>Q | No | EVA | |
| rs3392520602 | 841 | L>APAVLIYS* | No | EVA | |
| rs3388612519 | 841 | L>M | No | EVA | |
| rs3388615497 | 854 | G>R | No | EVA | |
| rs3388614442 | 926 | C>* | No | EVA | |
| rs3540877429 | 938 | V>A | No | EVA | |
| rs29874544 | 946 | S>P | No | EVA | |
| rs3388615628 | 950 | H>N | No | EVA | |
| rs3388606835 | 976 | P>L | No | EVA | |
| rs27298680 | 1103 | A>G | No | EVA | |
| rs3388610794 | 1150 | S>L | No | EVA | |
| rs3392560197 | 1224 | H>N | No | EVA | |
| rs27298695 | 1229 | G>A | No | EVA | |
| rs27298695 | 1229 | G>E | No | EVA | |
| rs3392213598 | 1247 | S>R | No | EVA | |
| rs3392353294 | 1249 | Q>R | No | EVA | |
| rs3388599065 | 1265 | I>F | No | EVA | |
| rs3392361651 | 1293 | D>A | No | EVA | |
| rs3388610625 | 1336 | A>T | No | EVA | |
| rs3540890268 | 1395 | S>L | No | EVA | |
| rs3388615623 | 1411 | S>A | No | EVA | |
| rs3388615532 | 1443 | P>A | No | EVA | |
| rs3388612477 | 1455 | H>R | No | EVA | |
| rs3388604824 | 1458 | L>F | No | EVA | |
| rs3388616335 | 1468 | G>R | No | EVA | |
| rs219845673 | 1470 | P>L | No | EVA | |
| rs3388610785 | 1471 | P>H | No | EVA | |
| rs3388614459 | 1542 | H>Q | No | EVA | |
| rs248877587 | 1547 | A>T | No | EVA | |
| rs262357175 | 1558 | V>I | No | EVA | |
| rs3388599029 | 1578 | M>I | No | EVA | |
| rs3388604815 | 1578 | M>L | No | EVA | |
| rs3388611758 | 1640 | Q>* | No | EVA |
No associated diseases with Q69ZK0
10 regional properties for Q69ZK0
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | Protein kinase domain | 935 - 1211 | IPR000719 |
| domain | MAM domain | 51 - 221 | IPR000998-1 |
| domain | MAM domain | 272 - 436 | IPR000998-2 |
| domain | Serine-threonine/tyrosine-protein kinase, catalytic domain | 935 - 1201 | IPR001245 |
| conserved_site | Tyrosine-protein kinase, receptor class II, conserved site | 1095 - 1103 | IPR002011 |
| repeat | Low-density lipoprotein (LDL) receptor class A repeat | 231 - 270 | IPR002172 |
| active_site | Tyrosine-protein kinase, active site | 1064 - 1076 | IPR008266 |
| binding_site | Protein kinase, ATP binding site | 941 - 969 | IPR017441 |
| domain | Tyrosine-protein kinase, catalytic domain | 935 - 1202 | IPR020635 |
| conserved_site | Low-density lipoprotein (LDL) receptor class A, conserved site | 245 - 268 | IPR023415 |
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| dendritic shaft | Cylindric portion of the dendrite, directly stemming from the perikaryon, and carrying the dendritic spines. |
| growth cone | The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic. |
| perinuclear region of cytoplasm | Cytoplasm situated near, or occurring around, the nucleus. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| GTPase activator activity | Binds to and increases the activity of a GTPase, an enzyme that catalyzes the hydrolysis of GTP. |
| guanyl-nucleotide exchange factor activity | Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions. |
| phospholipid binding | Binding to a phospholipid, a class of lipids containing phosphoric acid as a mono- or diester. |
11 GO annotations of biological process
| Name | Definition |
|---|---|
| G protein-coupled receptor signaling pathway | The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane. |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| neutrophil chemotaxis | The directed movement of a neutrophil cell, the most numerous polymorphonuclear leukocyte found in the blood, in response to an external stimulus, usually an infection or wounding. |
| positive regulation of cell adhesion | Any process that activates or increases the frequency, rate or extent of cell adhesion. |
| positive regulation of cell migration | Any process that activates or increases the frequency, rate or extent of cell migration. |
| positive regulation of substrate adhesion-dependent cell spreading | Any process that activates or increases the frequency, rate or extent of substrate adhesion-dependent cell spreading. |
| reactive oxygen species metabolic process | The chemical reactions and pathways involving a reactive oxygen species, any molecules or ions formed by the incomplete one-electron reduction of oxygen. They contribute to the microbicidal activity of phagocytes, regulation of signal transduction and gene expression, and the oxidative damage to biopolymers. |
| regulation of actin filament polymerization | Any process that modulates the frequency, rate or extent of the assembly of actin filaments by the addition of actin monomers to a filament. |
| regulation of dendrite development | Any process that modulates the frequency, rate or extent of dendrite development. |
| regulation of signaling | Any process that modulates the frequency, rate or extent of a signaling process. |
| T cell differentiation | The process in which a precursor cell type acquires characteristics of a more mature T-cell. A T cell is a type of lymphocyte whose definin characteristic is the expression of a T cell receptor complex. |
13 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q58DL7 | ARHGEF9 | Rho guanine nucleotide exchange factor 9 | Bos taurus (Bovine) | SS |
| O43307 | ARHGEF9 | Rho guanine nucleotide exchange factor 9 | Homo sapiens (Human) | SS |
| Q6XZF7 | DNMBP | Dynamin-binding protein | Homo sapiens (Human) | PR |
| Q9NR80 | ARHGEF4 | Rho guanine nucleotide exchange factor 4 | Homo sapiens (Human) | EV |
| A1IGU5 | ARHGEF37 | Rho guanine nucleotide exchange factor 37 | Homo sapiens (Human) | PR |
| Q96N96 | SPATA13 | Spermatogenesis-associated protein 13 | Homo sapiens (Human) | EV |
| Q70Z35 | PREX2 | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein | Homo sapiens (Human) | SS |
| Q8TCU6 | PREX1 | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein | Homo sapiens (Human) | EV |
| Q5DU57 | Spata13 | Spermatogenesis-associated protein 13 | Mus musculus (Mouse) | SS |
| Q7TNR9 | Arhgef4 | Rho guanine nucleotide exchange factor 4 | Mus musculus (Mouse) | EV |
| Q3UTH8 | Arhgef9 | Rho guanine nucleotide exchange factor 9 | Mus musculus (Mouse) | SS |
| Q570Y9 | Deptor | DEP domain-containing mTOR-interacting protein | Mus musculus (Mouse) | PR |
| Q3LAC4 | Prex2 | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein | Mus musculus (Mouse) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MEAPGSGGGD | GGGDPGGDGA | HPDARGPVSG | PCAAARDSER | QLRLRLCVLN | EILGTERDYV |
| 70 | 80 | 90 | 100 | 110 | 120 |
| GTLRFLQSAF | LQRIRQNVAD | SVEKGLTEEN | VKVLFSNIED | ILEVHKDFLA | ALEYCLHPEP |
| 130 | 140 | 150 | 160 | 170 | 180 |
| QSQHELGNVF | LKFKDKFCVY | EEYCSNHEKA | LRLLVELNKV | PAVRAFLLSC | MLLGGRKTTD |
| 190 | 200 | 210 | 220 | 230 | 240 |
| IPLEGYLLSP | IQRICKYPLL | LKELAKRTPG | KHPDHTAVQS | ALQAMKTVCS | NINETKRQME |
| 250 | 260 | 270 | 280 | 290 | 300 |
| KLEALEQLQS | HIEGWEGSNL | TDICTELLLQ | GNLLKISAGN | IQERAFFLFD | NLLVYCKRKS |
| 310 | 320 | 330 | 340 | 350 | 360 |
| RVTGSKKSTK | RTKSINGSLY | IFRGRINTEV | MEVENVEDGT | ADYHSNGYTV | TNGWKIHNTA |
| 370 | 380 | 390 | 400 | 410 | 420 |
| KNKWFVCMAK | TAEEKQKWLD | ALIREREQRE | SLKLGMERDA | YVMIAEKGEK | LYHMMMSKKV |
| 430 | 440 | 450 | 460 | 470 | 480 |
| NLIKDRRRKL | STVPKCFLGN | EFVAWLLEIG | EISKTEEGVN | LGQALLENGI | IHHVSDKHQF |
| 490 | 500 | 510 | 520 | 530 | 540 |
| KNEQVMYRFR | YDDGTYKARS | ELEDIMSKGV | RLYCRLHSLY | APVIKDRDYH | LKTYKSVVPG |
| 550 | 560 | 570 | 580 | 590 | 600 |
| SKLVDWLLAQ | GDCQTREEAV | ALGVGLCNNG | FMHHVLEKSE | FKDESQYFRF | HADEEMEGTS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| SKNKQLRNDF | KLVENILAKR | LLIPPQEDDY | GFDLEEKNKA | VVVKSVQRGS | LAEMAGLQAG |
| 670 | 680 | 690 | 700 | 710 | 720 |
| RKIYSINEDL | VFLRPFSEVE | TILNQFFCSR | RPLRLLVATK | AKETIKVPDH | PEALSFQIRG |
| 730 | 740 | 750 | 760 | 770 | 780 |
| TAPPCVFAVG | RGSEAVAAGL | CAGQCILKVN | GTSVANDGAL | EVLEHFQAFR | NHREEALGLY |
| 790 | 800 | 810 | 820 | 830 | 840 |
| QWVYHSHEDA | QLARASQGAP | DEDPQEDDQP | DSALPLLSLG | PQLSLHEDSA | VVSLTLDNVH |
| 850 | 860 | 870 | 880 | 890 | 900 |
| LEHGVVYEYM | STAGAKCHVL | EKIVEPRGCF | RLAAKILEAF | AVDDSIFVQN | CGRLMAMSSA |
| 910 | 920 | 930 | 940 | 950 | 960 |
| IVTMSHYEFH | NICDTKLESI | GQRIACYQEF | AAQLKSRVSP | PFKQASLEPH | PLCGLDFCPT |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| NCHVNLMEVS | YPKTTPSVGR | SFSIRFGRKP | SLIGLDPEQG | LNPMAYTQHC | ITTMAAPSWK |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| CSPAVDEDSQ | GQGLNDSSYG | SASGAPSQQD | RGLSFLLKQE | DREIQDAYLQ | LFTKLDVALK |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| EMKQYVTQIN | RLLSTITEPT | SAAPAPCDPS | LVEETSSSPP | VSEESEVDRT | DHSGIKKVCF |
| 1150 | 1160 | 1170 | 1180 | 1190 | 1200 |
| KVSEDEQEDS | GHDTMSYRDS | YSECNSNRDS | VLSYTSVRSN | SSYLGSDEMG | SGDELPCDMR |
| 1210 | 1220 | 1230 | 1240 | 1250 | 1260 |
| IPSDKQDKLH | GCLEHLFNQV | DSIHALLKGP | VMSRAFEETR | HFPMKHSWQE | FKQKEECTVR |
| 1270 | 1280 | 1290 | 1300 | 1310 | 1320 |
| GRNLIQISIQ | EDPWNLPSSI | RTLVDNIQQY | VEDGKNQLLL | ALLKCTDTEL | QLRRDAVFCQ |
| 1330 | 1340 | 1350 | 1360 | 1370 | 1380 |
| ALVAAVCTFS | EQLLAALDYR | YNNNGEYEES | SRDASRKWLE | QVAATGVLLH | WQSLLAPASV |
| 1390 | 1400 | 1410 | 1420 | 1430 | 1440 |
| KEERTMLEDI | WVTLSELDNV | TFSFKQLDEN | SVANTNVFYH | IEGSRQALKV | VFYLDGFHFS |
| 1450 | 1460 | 1470 | 1480 | 1490 | 1500 |
| RLPSRLEGGA | SLRLHTVLFT | KALESVEGPP | PPGNQAAEEL | QQEINAQSLE | KVQQYYRKLR |
| 1510 | 1520 | 1530 | 1540 | 1550 | 1560 |
| AFYLERSNLP | TDAGATAVKI | DQLIRPINAL | DELYRLMKTF | VHPKAGAAGS | LGAGLIPVSS |
| 1570 | 1580 | 1590 | 1600 | 1610 | 1620 |
| ELCYRLGACQ | ITMCGTGMQR | STLSVSLEQA | AILARSHGLL | PKCVMQATDI | MRKQGPRVEI |
| 1630 | 1640 | ||||
| LAKNLRIKDP | MPQGAPRLYQ | LCQPPVDGDL |