Q66HR2
SS
Gene name |
Mapre1 |
Protein name |
Microtubule-associated protein RP/EB family member 1 |
Names |
APC-binding protein EB1, End-binding protein 1, EB1 |
Species |
Rattus norvegicus (Rat) |
KEGG Pathway |
rno:114764 |
EC number |
|
Protein Class |
|
Descriptions
EB1 including a CH domain and the C-terminal dimerization domain is a plus-end tracking protein that binds to the plus-end of microtubules (MT) and regulates the dynamics of the microtubule cytoskeleton. The CH domain is responsible for MT-binding and inhibited by the binding of the CH domain and the central region of the C-terminal domain at the FYF motif. SxIP competes with the CH domain and binds on the FYF motif of the C-terminal domain, and activates the CH domain.
Autoinhibitory domains (AIDs)
Target domain |
14-117 (CH domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
References
- Kanaba T et al. (2013) "Microtubule-binding sites of the CH domain of EB1 and its autoinhibition revealed by NMR", Biochimica et biophysica acta, 1834, 499-507
- Komaki S et al. (2010) "Nuclear-localized subtype of end-binding 1 protein regulates spindle organization in Arabidopsis", Journal of cell science, 123, 451-9
Autoinhibited structure
Activated structure
1 structures for Q66HR2
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q66HR2-F1 | Predicted | AlphaFoldDB |
No variants for Q66HR2
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q66HR2 | |||||
No associated diseases with Q66HR2
5 regional properties for Q66HR2
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | CRIB domain | 69 - 126 | IPR000095 |
| active_site | Serine/threonine-protein kinase, active site | 383 - 395 | IPR008271 |
| binding_site | Protein kinase, ATP binding site | 274 - 297 | IPR017441 |
| domain | p21 activated kinase binding domain | 68 - 113 | IPR033923 |
| domain | p21-activated kinase 3, catalytic domain | 248 - 544 | IPR035063 |
Functions
14 GO annotations of cellular component
| Name | Definition |
|---|---|
| cell projection | A prolongation or process extending from a cell, e.g. a flagellum or axon. |
| cell projection membrane | The portion of the plasma membrane surrounding a plasma membrane bounded cell surface projection. |
| centrosome | A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle. |
| ciliary basal body | A membrane-tethered, short cylindrical array of microtubules and associated proteins found at the base of a eukaryotic cilium (also called flagellum) that is similar in structure to a centriole and derives from it. The cilium basal body is the site of assembly and remodelling of the cilium and serves as a nucleation site for axoneme growth. As well as anchoring the cilium, it is thought to provide a selective gateway regulating the entry of ciliary proteins and vesicles by intraflagellar transport. |
| cortical microtubule cytoskeleton | The portion of the microtubule cytoskeleton that lies just beneath the plasma membrane. |
| cytoplasmic microtubule | Any microtubule in the cytoplasm of a cell. |
| focal adhesion | A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ). |
| Golgi apparatus | A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways. |
| microtubule | Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle. |
| microtubule cytoskeleton | The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins. |
| microtubule organizing center | An intracellular structure that can catalyze gamma-tubulin-dependent microtubule nucleation and that can anchor microtubules by interacting with their minus ends, plus ends or sides. |
| microtubule plus-end | The growing (plus) end of a microtubule. In vitro, microtubules polymerize more quickly at the plus end than at the minus end. In vivo, microtubule growth occurs only at the plus end, and the plus end switches between periods of growth and shortening, a behavior known as dynamic instability. |
| mitotic spindle astral microtubule end | Any microtubule end that is part of a mitotic spindle astral microtubule. |
| spindle midzone | The area in the center of the spindle where the spindle microtubules from opposite poles overlap. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| identical protein binding | Binding to an identical protein or proteins. |
| microtubule plus-end binding | Binding to the plus end of a microtubule. |
| protein kinase binding | Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate. |
13 GO annotations of biological process
| Name | Definition |
|---|---|
| cell division | The process resulting in division and partitioning of components of a cell to form more cells; may or may not be accompanied by the physical separation of a cell into distinct, individually membrane-bounded daughter cells. |
| cell migration | The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues. Cell migration is a central process in the development and maintenance of multicellular organisms. |
| microtubule bundle formation | A process that results in a parallel arrangement of microtubules. |
| microtubule polymerization | The addition of tubulin heterodimers to one or both ends of a microtubule. |
| negative regulation of microtubule polymerization | Any process that stops, prevents, or reduces the frequency, rate or extent of microtubule polymerization. |
| non-motile cilium assembly | The aggregation, arrangement and bonding together of a set of components to form a non-motile cilium. |
| positive regulation of microtubule plus-end binding | Any process that activates or increases the frequency, rate or extent of microtubule plus-end binding. |
| positive regulation of microtubule polymerization | Any process that activates or increases the frequency, rate or extent of microtubule polymerization. |
| protein localization to centrosome | A process in which a protein is transported to, or maintained at, the centrosome. |
| protein localization to microtubule | A process in which a protein is transported to, or maintained at, a microtubule. |
| protein localization to microtubule plus-end | A process in which a protein is transported to, or maintained in, a location at a microtubule plus-end. |
| regulation of microtubule polymerization or depolymerization | Any process that modulates the frequency, rate or extent of microtubule polymerization or depolymerization by the addition or removal of tubulin heterodimers from a microtubule. |
| spindle assembly | The aggregation, arrangement and bonding together of a set of components to form the spindle, the array of microtubules and associated molecules that serves to move duplicated chromosomes apart. |
12 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q3ZBD9 | MAPRE1 | Microtubule-associated protein RP/EB family member 1 | Bos taurus (Bovine) | SS |
| Q5ZLC7 | MAPRE1 | Microtubule-associated protein RP/EB family member 1 | Gallus gallus (Chicken) | SS |
| Q15555 | MAPRE2 | Microtubule-associated protein RP/EB family member 2 | Homo sapiens (Human) | PR |
| Q9UPY8 | MAPRE3 | Microtubule-associated protein RP/EB family member 3 | Homo sapiens (Human) | SS |
| Q15691 | MAPRE1 | Microtubule-associated protein RP/EB family member 1 | Homo sapiens (Human) | EV |
| Q8R001 | Mapre2 | Microtubule-associated protein RP/EB family member 2 | Mus musculus (Mouse) | PR |
| Q61166 | Mapre1 | Microtubule-associated protein RP/EB family member 1 | Mus musculus (Mouse) | SS |
| Q6PER3 | Mapre3 | Microtubule-associated protein RP/EB family member 3 | Mus musculus (Mouse) | SS |
| Q5XIT1 | Mapre3 | Microtubule-associated protein RP/EB family member 3 | Rattus norvegicus (Rat) | SS |
| Q7XJ60 | EB1A | Microtubule-associated protein RP/EB family member 1A | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FJJ5 | EB1B | Microtubule-associated protein RP/EB family member 1B | Arabidopsis thaliana (Mouse-ear cress) | EV |
| Q6P848 | mapre1 | Microtubule-associated protein RP/EB family member 1 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAVNVYSTSV | TSDNLSRHDM | LAWINESLQL | NLTKIEQLCS | GAAYCQFMDM | LFPGSIALKK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VKFQAKLEHE | YIQNFKILQA | GFKRMGVDKI | IPVDKLVKGK | FQDNFEFVQW | FKKFFDANYD |
| 130 | 140 | 150 | 160 | 170 | 180 |
| GKEYDPVAAR | QGQETAVAPS | LVAPALSKPK | KPLGSGSAAP | QRPIATQRTT | AAPKAGPGMV |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RKNPGMGNGD | DEAAELMQQV | KVLKLTVEDL | EKERDFYFGK | LRNIELICQE | NEGENDPVLQ |
| 250 | 260 | ||||
| RIVDILYATD | EGFVIPDEGG | PQEEQEEY |