Q66H84

SS
Gene name
Mapkapk3
Protein name
MAP kinase-activated protein kinase 3
Names
MAPK-activated protein kinase 3 , MAPKAP kinase 3 , MAPKAP-K3 , MAPKAPK-3 , MK-3 , EC 2.7.11.1
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:315994
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

MAPKAPK2 is a stress-activated serine/threonine-protein kinase. MAPKAPK2, contains an autoinhibitory α-helix overlapping with a leucine-rich nuclear export signal (NES), and also lies close to the nuclear localization signal (NLS). The closed conformation of MAPKAPK2 prevents exportin binding to NES. Phosphorylation of MAPKAPK2 at threonine 317 induces a conformational change, thus unmasks and increases the activity of NES.

Autoinhibitory domains (AIDs)

313-333 (Autoinhibitory A-helix) SS

Target domain

5-306 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

188-209 (Activation loop from InterPro)

Target domain

45-306 (Protein kinase domain)

Relief mechanism

Assay

188-209 (Activation loop from InterPro)

Target domain

45-306 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q66H84

Entry ID Method Resolution Chain Position Source
AF-Q66H84-F1 Predicted AlphaFoldDB

No variants for Q66H84

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q66H84

No associated diseases with Q66H84

7 regional properties for Q66H84

Type Name Position InterPro Accession
domain EF-hand domain 237 - 288 IPR002048
domain FAD-binding 8 615 - 729 IPR013112
domain Ferric reductase, NAD binding domain 736 - 903 IPR013121
domain Ferric reductase transmembrane component-like domain 416 - 572 IPR013130
domain NADPH oxidase Respiratory burst 157 - 256 IPR013623
domain FAD-binding domain, ferredoxin reductase-type 611 - 732 IPR017927
binding_site EF-Hand 1, calcium-binding site 266 - 278 IPR018247

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Predominantly located in the nucleus, when activated it translocates to the cytoplasm
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
calcium-dependent protein serine/threonine kinase activity Calcium-dependent catalysis of the reactions
calmodulin binding Binding to calmodulin, a calcium-binding protein with many roles, both in the calcium-bound and calcium-free states.
calmodulin-dependent protein kinase activity Calmodulin-dependent catalysis of the reactions
mitogen-activated protein kinase binding Binding to a mitogen-activated protein kinase.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

6 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
macropinocytosis An endocytosis process that results in the uptake of liquid material by cells from their external environment by the 'ruffling' of the cell membrane to form heterogeneously sized intracellular vesicles called macropinosomes, which can be up to 5 micrometers in size.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
response to cytokine Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cytokine stimulus.
response to lipopolysaccharide Any process that results in a change in state or activity of an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a lipopolysaccharide stimulus; lipopolysaccharide is a major component of the cell wall of gram-negative bacteria.
toll-like receptor signaling pathway The series of molecular signals initiated by a ligand binding to a toll-like receptor of a target cell. Toll-like receptors directly bind pattern motifs from a variety of microbial sources to initiate an innate immune response.

10 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3SYZ2 MAPKAPK3 MAP kinase-activated protein kinase 3 Bos taurus (Bovine) SS
P49071 MAPk-Ak2 MAP kinase-activated protein kinase 2 Drosophila melanogaster (Fruit fly) SS
P49137 MAPKAPK2 MAP kinase-activated protein kinase 2 Homo sapiens (Human) EV
Q8IW41 MAPKAPK5 MAP kinase-activated protein kinase 5 Homo sapiens (Human) SS
Q16644 MAPKAPK3 MAP kinase-activated protein kinase 3 Homo sapiens (Human) SS
O54992 Mapkapk5 MAP kinase-activated protein kinase 5 Mus musculus (Mouse) EV
P49138 Mapkapk2 MAP kinase-activated protein kinase 2 Mus musculus (Mouse) EV
Q3UMW7 Mapkapk3 MAP kinase-activated protein kinase 3 Mus musculus (Mouse) SS
P13234 Camk4 Calcium/calmodulin-dependent protein kinase type IV Rattus norvegicus (Rat) SS
Q965G5 mak-2 MAP kinase-activated protein kinase mak-2 Caenorhabditis elegans SS
10 20 30 40 50 60
MDGETAGEKG SLVPQPGALG APALGGAPAP GVRREPKKYA VTDDYQLSKQ VLGLGVNGKV
70 80 90 100 110 120
LECYHRRSGQ KCALKLLYDS PKARQEVDHH WQASGGPHIV RILDVYENMH HGKRCLLIVM
130 140 150 160 170 180
ECMEGGELFS RIQERGDQAF TEREAAEIMR DIGTAIQFLH SQNIAHRDVK PENLLYTSKE
190 200 210 220 230 240
KDAVLKLTDF GFAKETTQNA LQTPCYTPYY VAPEVLGPEK YDKSCDMWSL GVIMYILLCG
250 260 270 280 290 300
FPPFYSNTGQ AISPGMKRRI RLGQYGFPKP EWADVSEDAK QLIRLLLKTD PTERLTIMQF
310 320 330 340 350 360
MNHPWINQSM EVPQTPLHTA RVLEEDKDHW DDVKEEMTSA LATMRVDYDQ VKIKDLKTSN
370 380
NRLLNKRRKK QGGSSSASPG CNNQ