Q64727
SS
Gene name |
Vcl |
Protein name |
Vinculin |
Names |
Metavinculin |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:22330 |
EC number |
|
Protein Class |
|
Descriptions
Vinculin is a highly conserved intracellular protein with a crucial role in the maintenance and regulation of cell adhesion and migration. N-terminal globular head (Vh) is composed of subdomains (D1-D4). The C-terminal tail (Vt) binds F-actin. A strap sequence (878-890) between Pro-rich region and Vt packs against the Vt and thereby masks the F-actin-binding domain in Vt.
Autoinhibitory domains (AIDs)
Target domain |
896-997 (Vt domain) |
Relief mechanism |
Ligand binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
2 structures for Q64727
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| 5Y04 | X-ray | 285 A | A | 1-250 | PDB |
| AF-Q64727-F1 | Predicted | AlphaFoldDB |
40 variants for Q64727
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs3389309314 | 43 | P>S | No | EVA | |
| rs3389276824 | 82 | E>K | No | EVA | |
| rs3389303123 | 91 | A>E | No | EVA | |
| rs216444011 | 97 | S>A | No | EVA | |
| rs3389312984 | 114 | G>E | No | EVA | |
| rs3389276891 | 179 | Q>H | No | EVA | |
| rs3389276844 | 185 | Q>H | No | EVA | |
| rs3389309386 | 297 | Q>* | No | EVA | |
| rs49456828 | 304 | D>Y | No | EVA | |
| rs48351403 | 330 | Q>H | No | EVA | |
| rs3389323407 | 366 | K>I | No | EVA | |
| rs3389313016 | 384 | I>T | No | EVA | |
| rs3389323321 | 434 | S>F | No | EVA | |
| rs48379692 | 500 | A>V | No | EVA | |
| rs3389266108 | 612 | A>T | No | EVA | |
| rs3389266196 | 618 | D>H | No | EVA | |
| rs3389276827 | 626 | F>Y | No | EVA | |
| rs3389311999 | 630 | A>T | No | EVA | |
| rs3389313160 | 643 | T>I | No | EVA | |
| rs3389238248 | 670 | E>G | No | EVA | |
| rs3389303145 | 691 | A>T | No | EVA | |
| rs3389238238 | 716 | A>D | No | EVA | |
| rs3405172607 | 720 | K>R | No | EVA | |
| rs3404836493 | 727 | E>A | No | EVA | |
| rs3389276854 | 750 | V>I | No | EVA | |
| rs3389312960 | 764 | L>M | No | EVA | |
| rs3389312985 | 793 | T>K | No | EVA | |
| rs3404105176 | 796 | P>S | No | EVA | |
| rs3404861942 | 803 | A>P | No | EVA | |
| rs3389313022 | 810 | D>G | No | EVA | |
| rs3389306993 | 865 | L>P | No | EVA | |
| rs3389293979 | 868 | G>C | No | EVA | |
| rs3389309339 | 873 | P>L | No | EVA | |
| rs3389283740 | 875 | P>L | No | EVA | |
| rs3389294005 | 899 | M>I | No | EVA | |
| rs47101659 | 900 | M>L | No | EVA | |
| rs3389326599 | 916 | G>D | No | EVA | |
| rs3389307335 | 1004 | T>I | No | EVA | |
| rs3389311924 | 1037 | T>S | No | EVA | |
| rs3389313010 | 1049 | R>G | No | EVA |
No associated diseases with Q64727
Functions
22 GO annotations of cellular component
| Name | Definition |
|---|---|
| actin cytoskeleton | The part of the cytoskeleton (the internal framework of a cell) composed of actin and associated proteins. Includes actin cytoskeleton-associated complexes. |
| adherens junction | A cell-cell junction composed of the epithelial cadherin-catenin complex. The epithelial cadherins, or E-cadherins, of each interacting cell extend through the plasma membrane into the extracellular space and bind to each other. The E-cadherins bind to catenins on the cytoplasmic side of the membrane, where the E-cadherin-catenin complex binds to cytoskeletal components and regulatory and signaling molecules. |
| brush border | The dense covering of microvilli on the apical surface of an epithelial cell in tissues such as the intestine, kidney, and choroid plexus; the microvilli aid absorption by increasing the surface area of the cell. |
| cell projection | A prolongation or process extending from a cell, e.g. a flagellum or axon. |
| cell-cell contact zone | Extended zone of intimate apposition between two cells containing one or more types of intercellular junctions, e.g., the intercalated disk of muscle. |
| cell-cell junction | A cell junction that forms a connection between two or more cells of an organism; excludes direct cytoplasmic intercellular bridges, such as ring canals in insects. |
| costamere | Regular periodic sub membranous arrays of vinculin in skeletal and cardiac muscle cells, these arrays link Z-discs to the sarcolemma and are associated with links to extracellular matrix. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytoskeleton | A cellular structure that forms the internal framework of eukaryotic and prokaryotic cells. The cytoskeleton includes intermediate filaments, microfilaments, microtubules, the microtrabecular lattice, and other structures characterized by a polymeric filamentous nature and long-range order within the cell. The various elements of the cytoskeleton not only serve in the maintenance of cellular shape but also have roles in other cellular functions, including cellular movement, cell division, endocytosis, and movement of organelles. |
| fascia adherens | A cell-cell junction that contains the transmembrane protein N-cadherin, which interacts with identical molecules from neighbouring cells to form a tight mechanical intercellular link; forms a large portion of the intercalated disc, the structure at which myofibrils terminate in cardiomyocytes. |
| focal adhesion | A cell-substrate junction that anchors the cell to the extracellular matrix and that forms a point of termination of actin filaments. In insects focal adhesion has also been referred to as hemi-adherens junction (HAJ). |
| inner dense plaque of desmosome | The desmosomal part containing the C-termini of desmoplakins which interact with the keratin intermediate filaments, serving to tether the intermediate filaments to the plasma membrane. |
| intercalated disc | A complex cell-cell junction at which myofibrils terminate in cardiomyocytes; mediates mechanical and electrochemical integration between individual cardiomyocytes. The intercalated disc contains regions of tight mechanical attachment (fasciae adherentes and desmosomes) and electrical coupling (gap junctions) between adjacent cells. |
| outer dense plaque of desmosome | The desmosomal part containing plakoglobins, plakophilins, the N-termini of desmoplakins, as well as the cytoplasmic tails of the desmosomal cadherins, which together attach the plaque to the plasma membrane. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
| podosome | An actin-rich adhesion structure characterized by formation upon cell substrate contact and localization at the substrate-attached part of the cell, contain an F-actin-rich core surrounded by a ring structure containing proteins such as vinculin and talin, and have a diameter of 0.5 mm. |
| podosome ring | The ring structure surrounding the podosome core, containing proteins such as vinculin and talin. |
| protein-containing complex | A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. |
| sarcolemma | The outer membrane of a muscle cell, consisting of the plasma membrane, a covering basement membrane (about 100 nm thick and sometimes common to more than one fiber), and the associated loose network of collagen fibers. |
| terminal web | An actin-rich cytoskeletal network located beneath the microvilli of the apical plasma membrane of polarized epithelial cells. In addition to actin filaments, the terminal web may contain actin-binding proteins, myosin motor proteins, and intermediate filaments. The terminal web can function as a contractile structure that influences the spatial distribution of microvilli as well as the development and morphogenesis of tissues containing polarized epithelial cells. |
| Z disc | Platelike region of a muscle sarcomere to which the plus ends of actin filaments are attached. |
| zonula adherens | A cell-cell adherens junction which forms a continuous belt near the apex of epithelial cells. |
7 GO annotations of molecular function
| Name | Definition |
|---|---|
| actin filament binding | Binding to an actin filament, also known as F-actin, a helical filamentous polymer of globular G-actin subunits. |
| alpha-catenin binding | Binding to catenin complex alpha subunit. |
| beta-catenin binding | Binding to a catenin beta subunit. |
| dystroglycan binding | Binding to dystroglycan, a glycoprotein found in non-muscle tissues as well as in muscle tissues, often in association with dystrophin. The native dystroglycan cleaved into two non-covalently associated subunits, alpha (N-terminal) and beta (C-terminal). |
| small GTPase binding | Binding to a small monomeric GTPase. |
| structural molecule activity | The action of a molecule that contributes to the structural integrity of a complex. |
| ubiquitin protein ligase binding | Binding to a ubiquitin protein ligase enzyme, any of the E3 proteins. |
12 GO annotations of biological process
| Name | Definition |
|---|---|
| adherens junction assembly | The aggregation, arrangement and bonding together of a set of components to form an adherens junction. An adherens junction is a cell-cell junction composed of the epithelial cadherin-catenin complex at which the cytoplasmic face of the plasma membrane is attached to actin filaments. |
| apical junction assembly | The formation of an apical junction, a functional unit located near the cell apex at the points of contact between epithelial cells composed of the tight junction, the zonula adherens junction and the desmosomes, by the aggregation, arrangement and bonding together of its constituents. |
| axon extension | Long distance growth of a single axon process involved in cellular development. |
| cell adhesion | The attachment of a cell, either to another cell or to an underlying substrate such as the extracellular matrix, via cell adhesion molecules. |
| epithelial cell-cell adhesion | The attachment of an epithelial cell to another epithelial cell via adhesion molecules. |
| lamellipodium assembly | Formation of a lamellipodium, a thin sheetlike extension of the surface of a migrating cell. |
| morphogenesis of an epithelium | The process in which the anatomical structures of epithelia are generated and organized. An epithelium consists of closely packed cells arranged in one or more layers, that covers the outer surfaces of the body or lines any internal cavity or tube. |
| protein localization to cell surface | A process in which a protein is transported to, or maintained in, a location within the external part of the cell wall and/or plasma membrane. |
| regulation of cell migration | Any process that modulates the frequency, rate or extent of cell migration. |
| regulation of establishment of endothelial barrier | Any process that modulates the frequency, rate or extent of establishment of endothelial barrier. |
| regulation of focal adhesion assembly | Any process that modulates the frequency, rate or extent of focal adhesion formation, the establishment and maturation of focal adhesions. |
| regulation of protein localization to adherens junction | Any process that modulates the frequency, rate or extent of protein localization to adherens junction. An adherens junction is a cell-cell junction composed of the epithelial cadherin-catenin complex at which the cytoplasmic face of the plasma membrane is attached to actin filaments. |
16 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q3MHM6 | CTNNA1 | Catenin alpha-1 | Bos taurus (Bovine) | SS |
| P30997 | CTNNA2 | Catenin alpha-2 | Gallus gallus (Chicken) | SS |
| P12003 | VCL | Vinculin | Gallus gallus (Chicken) | EV |
| P35220 | alpha-Cat | Catenin alpha | Drosophila melanogaster (Fruit fly) | SS |
| P26232 | CTNNA2 | Catenin alpha-2 | Homo sapiens (Human) | SS |
| P35221 | CTNNA1 | Catenin alpha-1 | Homo sapiens (Human) | EV |
| Q9UI47 | CTNNA3 | Catenin alpha-3 | Homo sapiens (Human) | SS |
| P18206 | VCL | Vinculin | Homo sapiens (Human) | SS |
| Q61301 | Ctnna2 | Catenin alpha-2 | Mus musculus (Mouse) | EV |
| Q65CL1 | Ctnna3 | Catenin alpha-3 | Mus musculus (Mouse) | EV |
| P26231 | Ctnna1 | Catenin alpha-1 | Mus musculus (Mouse) | EV |
| P26234 | VCL | Vinculin | Sus scrofa (Pig) | SS |
| P85972 | Vcl | Vinculin | Rattus norvegicus (Rat) | SS |
| P90947 | hmp-1 | Alpha-catenin-like protein hmp-1 | Caenorhabditis elegans | SS |
| A4IGI7 | ctnna2 | Catenin alpha-2 | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | SS |
| B7ZC77 | Ctnna2 | Catenin alpha-2 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MPVFHTRTIE | SILEPVAQQI | SHLVIMHEEG | EVDGKAIPDL | TAPVAAVQAA | VSNLVRVGKE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| TVQTTEDQIL | KRDMPPAFIK | VENACTKLVQ | AAQMLQSDPY | SVPARDYLID | GSRGILSGTS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| DLLLTFDEAE | VRKIIRVCKG | ILEYLTVAEV | VETMEDLVTY | TKNLGPGMTK | MAKMIDERQQ |
| 190 | 200 | 210 | 220 | 230 | 240 |
| ELTHQEHRVM | LVNSMNTVKE | LLPVLISAMK | IFVTTKNSKN | QGIEEALKNR | NFTVEKMSAE |
| 250 | 260 | 270 | 280 | 290 | 300 |
| INEIIRVLQL | TSWDEDAWAS | KDTEAMKRAL | ASIDSKLNQA | KGWLRDPNAS | PGDAGEQAIR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| QILDEAGKVG | ELCAGKERRE | ILGTCKMLGQ | MTDQVADLRA | RGQGASPVAM | QKAQQVSQGL |
| 370 | 380 | 390 | 400 | 410 | 420 |
| DVLTAKVENA | ARKLEAMTNS | KQSIAKKIDA | AQNWLADPNG | GPEGEEQIRG | ALAEARKIAE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| LCDDPKERDD | ILRSLGEIAA | LTSKLGDLRR | QGKGDSPEAR | ALAKQVATAL | QNLQTKTNRA |
| 490 | 500 | 510 | 520 | 530 | 540 |
| VANSRPAKAA | VHLEGKIEQA | QRWIDNPTVD | DRGVGQAAIR | GLVAEGHRLA | NVMMGPYRQD |
| 550 | 560 | 570 | 580 | 590 | 600 |
| LLAKCDRVDQ | LTAQLADLAA | RGEGESPQAR | ALASQLQDSL | KDLKAQMQEA | MTQEVSDVFS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| DTTTPIKLLA | VAATAPPDAP | NREEVFDERA | ANFENHSGRL | GATAEKAAAV | GTANKSTVEG |
| 670 | 680 | 690 | 700 | 710 | 720 |
| IQASVKTARE | LTPQVISAAR | ILLRNPGNQA | AYEHFETMKN | QWIDNVEKMT | GLVDEAIDTK |
| 730 | 740 | 750 | 760 | 770 | 780 |
| SLLDASEEAI | KKDLDKCKVA | MANIQPQMLV | AGATSIARRA | NRILLVAKRE | VENSEDPKFR |
| 790 | 800 | 810 | 820 | 830 | 840 |
| EAVKAASDEL | SKTISPMVMD | AKAVAGNISD | PGLQKSFLDS | GYRILGAVAK | VREAFQPQEP |
| 850 | 860 | 870 | 880 | 890 | 900 |
| DFPPPPPDLE | QLRLTDELAP | PKPPLPEGEV | PPPRPPPPEE | KDEEFPEQKA | GEVINQPMMM |
| 910 | 920 | 930 | 940 | 950 | 960 |
| AARQLHDEAR | KWSSKGNDII | AAAKRMALLM | AEMSRLVRGG | SGTKRALIQC | AKDIAKASDE |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| VTRLAKEVAK | QCTDKRIRTN | LLQVCERIPT | ISTQLKILST | VKATMLGRTN | ISDEESEQAT |
| 1030 | 1040 | 1050 | 1060 | ||
| EMLVHNAQNL | MQSVKETVRE | AEAASIKIRT | DAGFTLRWVR | KTPWYQ |