Q64287

EV
Gene name
Irf4 (Spip)
Protein name
Interferon regulatory factor 4
Names
IRF-4, Lymphocyte-specific interferon regulatory factor, LSIRF, NF-EM5, PU.1 interaction partner, Transcriptional activator PIP
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:16364
EC number
Protein Class
INTERFERON REGULATORY FACTOR (PTHR11949)

Descriptions

IRF4 is a transcription factor regulating immune cell development, obesity-induced inflammation, and so forth. The low DNA binding affinity of IRF4 has been attributed to the presence of an C-terminal autoinhibitory region residing in the last 30 residues of the C-terminal interferon activation domain (IAD). It has been suggested that this region physically interacts with the N-terminal helix-turn-helix DNA-binding domain and maintains the protein in an autoinhibited state. Upon interaction with a binding partner, the inhibitory mechanism is relieved, allowing IRF4 to bind its recognition DNA sequence.

Autoinhibitory domains (AIDs)

420-450 (C-terminal autoinhibitory region) EV

Target domain

20-139 (DNA-binding domain)

Relief mechanism

Partner binding

Assay

Deletion assay, Mutagenesis experiment, Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q64287

Entry ID Method Resolution Chain Position Source
5BVI X-ray 260 A A/B 238-420 PDB
AF-Q64287-F1 Predicted AlphaFoldDB

2 variants for Q64287

Variant ID(s) Position Change Description Diseaes Association Provenance
rs265187539 186 S>A No Ensembl
rs217122175 269 T>S No Ensembl

No associated diseases with Q64287

3 regional properties for Q64287

Type Name Position InterPro Accession
domain Interferon regulatory factor, DNA-binding domain 17 - 130 IPR001346
domain Interferon regulatory factor-3 249 - 418 IPR019471
conserved_site Interferon regulatory factor, conserved site 42 - 75 IPR019817

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family PTHR11949 INTERFERON REGULATORY FACTOR
PANTHER Subfamily PTHR11949:SF6 INTERFERON REGULATORY FACTOR 4
PANTHER Protein Class DNA-binding transcription factor
helix-turn-helix transcription factor
winged helix/forkhead transcription factor
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleosome A complex comprised of DNA wound around a multisubunit core and associated proteins, which forms the primary packing unit of DNA into higher order structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

8 GO annotations of molecular function

Name Definition
cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by some RNA polymerase. The proximal promoter is in cis with and relatively close to the core promoter.
DNA-binding transcription activator activity, RNA polymerase II-specific A DNA-binding transcription factor activity that activates or increases transcription of specific gene sets transcribed by RNA polymerase II.
DNA-binding transcription factor activity A transcription regulator activity that modulates transcription of gene sets via selective and non-covalent binding to a specific double-stranded genomic DNA sequence (sometimes referred to as a motif) within a cis-regulatory region. Regulatory regions include promoters (proximal and distal) and enhancers. Genes are transcriptional units, and include bacterial operons.
DNA-binding transcription factor activity, RNA polymerase II-specific A DNA-binding transcription factor activity that modulates the transcription of specific gene sets transcribed by RNA polymerase II.
RNA polymerase II cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.
sequence-specific DNA binding Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding.
sequence-specific double-stranded DNA binding Binding to double-stranded DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA, e.g. promotor binding or rDNA binding.
transcription coactivator activity A transcription coregulator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound DNA-binding transcription factor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coactivators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator.

16 GO annotations of biological process

Name Definition
defense response to protozoan Reactions triggered in response to the presence of a protozoan that act to protect the cell or organism.
histone H3 acetylation The modification of histone H3 by the addition of an acetyl group.
histone H4 acetylation The modification of histone H4 by the addition of an acetyl group.
immune system process Any process involved in the development or functioning of the immune system, an organismal system for calibrated responses to potential internal or invasive threats.
myeloid dendritic cell differentiation The process in which a monocyte acquires the specialized features of a dendritic cell, an immunocompetent cell of the lymphoid and hemopoietic systems and skin.
negative regulation of toll-like receptor signaling pathway Any process that stops, prevents, or reduces the frequency, rate, or extent of toll-like receptor signaling pathway.
positive regulation of cold-induced thermogenesis Any process that activates or increases the frequency, rate or extent of cold-induced thermogenesis.
positive regulation of DNA binding Any process that increases the frequency, rate or extent of DNA binding. DNA binding is any process in which a gene product interacts selectively with DNA (deoxyribonucleic acid).
positive regulation of interleukin-10 production Any process that activates or increases the frequency, rate, or extent of interleukin-10 production.
positive regulation of interleukin-13 production Any process that activates or increases the frequency, rate, or extent of interleukin-13 production.
positive regulation of interleukin-2 production Any process that activates or increases the frequency, rate, or extent of interleukin-2 production.
positive regulation of interleukin-4 production Any process that activates or increases the frequency, rate, or extent of interleukin-4 production.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of transcription, DNA-templated Any process that activates or increases the frequency, rate or extent of cellular DNA-templated transcription.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
T-helper 17 cell lineage commitment The process in which a CD4-positive, alpha-beta T cell becomes committed to becoming a T-helper 17 cell, a CD4-positive, alpha-beta T cell with the phenotype RORgamma-t-positive that produces IL-17.

6 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q15306 IRF4 Interferon regulatory factor 4 Homo sapiens (Human) SS
Q61179 Irf9 Interferon regulatory factor 9 Mus musculus (Mouse) PR
P56477 Irf5 Interferon regulatory factor 5 Mus musculus (Mouse) SS
P70671 Irf3 Interferon regulatory factor 3 Mus musculus (Mouse) SS
P70434 Irf7 Interferon regulatory factor 7 Mus musculus (Mouse) SS
P23611 Irf8 Interferon regulatory factor 8 Mus musculus (Mouse) PR
10 20 30 40 50 60
MNLETGSRGS EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSVF RIPWKHAGKQ
70 80 90 100 110 120
DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA LNKSNDFEEL VERSQLDISD
130 140 150 160 170 180
PYKVYRIVPE GAKKGAKQLT LDDTQMAMGH PYPMTAPYGS LPAQQVHNYM MPPHDRSWRD
190 200 210 220 230 240
YAPDQSHPEI PYQCPVTFGP RGHHWQGPSC ENGCQVTGTF YACAPPESQA PGIPIEPSIR
250 260 270 280 290 300
SAEALALSDC RLHICLYYRD ILVKELTTTS PEGCRISHGH TYDVSNLDQV LFPYPDDNGQ
310 320 330 340 350 360
RKNIEKLLSH LERGLVLWMA PDGLYAKRLC QSRIYWDGPL ALCSDRPNKL ERDQTCKLFD
370 380 390 400 410 420
TQQFLSELQV FAHHGRPAPR FQVTLCFGEE FPDPQRQRKL ITAHVEPLLA RQLYYFAQQN
430 440
TGHFLRGYEL PEHVTTPDYH RSLRHSSIQE