Q63803

SS
Gene name
Gnas (Gnas1)
Protein name
Guanine nucleotide-binding protein G
Names
s subunit alpha isoforms XLas , Adenylate cyclase-stimulating G alpha protein , Extra large alphas protein , XLalphas , G-alpha-8
Species
Rattus norvegicus (Rat)
KEGG Pathway
EC number
Protein Class

Descriptions

The Gα subunits of heterotrimeric G proteins are GTP hydrolyases which, upon receptor activation, bind to GTP and regulate effector molecules. Gα-catalyzed hydrolysis of GTP to GDP releases the Gα-GDP product complex from effector and allows its sequestration by inhibitory Gβγ subunits. In Gia1-GppNHp complex, Gln204 of Gia1 interacts with nucleophilic water (Wnuc), participating directly in the Gia1-GTP ground state but must reorient to stabilize the transition state. RGS4, a member of the RGS family of G protein stimulatory factors, accelerates hydrolysis of GTP by Gia1 by destabilizing the ground-state conformation of Gln204. Insertion of Asn128 of RGS4 into the active site enhances catalysis by aiding in binding, orienting, and polarizing Wnuc in the pre-transition state complex. Asn128 of RGS4 displaces the side chain of Gln204 from its anti-catalytic position in the ground state, freeing it to participate in stabilization of the transition state.

Autoinhibitory domains (AIDs)

977-978 (Gln204) SS

Target domain

952-1139 (P-loop containing nucleoside triphosphate hydrolase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q63803

Entry ID Method Resolution Chain Position Source
AF-Q63803-F1 Predicted AlphaFoldDB

No variants for Q63803

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q63803

No associated diseases with Q63803

3 regional properties for Q63803

Type Name Position InterPro Accession
domain Protein kinase domain 43 - 304 IPR000719
active_site Serine/threonine-protein kinase, active site 162 - 174 IPR008271
binding_site Protein kinase, ATP binding site 50 - 73 IPR017441

Functions

Description
EC Number
Subcellular Localization
  • Cell membrane ; Peripheral membrane protein
  • Apical cell membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

15 GO annotations of cellular component

Name Definition
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
COPI-coated Golgi to ER transport vesicle A vesicle that mediates transport from the Golgi to the endoplasmic reticulum.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
dendrite A neuron projection that has a short, tapering, morphology. Dendrites receive and integrate signals from other neurons or from sensory stimuli, and conduct nerve impulses towards the axon or the cell body. In most neurons, the impulse is conveyed from dendrites to axon via the cell body, but in some types of unipolar neuron, the impulse does not travel via the cell body.
endosome A vacuole to which materials ingested by endocytosis are delivered.
heterotrimeric G-protein complex Any of a family of heterotrimeric GTP-binding and hydrolyzing proteins; they belong to a superfamily of GTPases that includes monomeric proteins such as EF-Tu and RAS. Heterotrimeric G-proteins consist of three subunits; the alpha subunit contains the guanine nucleotide binding site and possesses GTPase activity; the beta and gamma subunits are tightly associated and function as a beta-gamma heterodimer; extrinsic plasma membrane proteins (cytoplasmic face) that function as a complex to transduce signals from G protein-coupled receptors to an effector protein.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it.
neuronal cell body The portion of a neuron that includes the nucleus, but excludes cell projections such as axons and dendrites.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
recycling endosome An organelle consisting of a network of tubules that functions in targeting molecules, such as receptors transporters and lipids, to the plasma membrane.
ruffle Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.
trans-Golgi network membrane The lipid bilayer surrounding any of the compartments that make up the trans-Golgi network.

17 GO annotations of molecular function

Name Definition
adenylate cyclase activator activity Binds to and increases the activity of adenylate cyclase.
adenylate cyclase regulator activity Binds to and modulates the activity of adenylate cyclase.
alpha-tubulin binding Binding to the microtubule constituent protein alpha-tubulin.
beta-2 adrenergic receptor binding Binding to a beta-2 adrenergic receptor.
corticotropin-releasing hormone receptor 1 binding Binding to a corticotropin-releasing hormone receptor 1 (CRHR1). CRHR1 is the major subtype in the pituitary corticotroph, and mediates the stimulatory actions of corticotropin-releasing hormone on corticotropin hormone secretion. CRHR1 are also located in cortical areas of the brain, cerebellum and limbic system.
D1 dopamine receptor binding Binding to a D1 dopamine receptor.
G protein activity A molecular function regulator that cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular processes. Intrinsic GTPase activity returns the G protein to its GDP-bound state. The return to the GDP-bound state can be accelerated by the action of a GTPase-activating protein (GAP).
G-protein alpha-subunit binding Binding to a G-protein alpha subunit. The alpha subunit binds a guanine nucleotide.
G-protein beta-subunit binding Binding to a G-protein beta subunit.
G-protein beta/gamma-subunit complex binding Binding to a complex of G-protein beta/gamma subunits.
GTP binding Binding to GTP, guanosine triphosphate.
GTPase activity Catalysis of the reaction
insulin-like growth factor receptor binding Binding to an insulin-like growth factor receptor.
ionotropic glutamate receptor binding Binding to an ionotropic glutamate receptor. Ionotropic glutamate receptors bind glutamate and exert an effect through the regulation of ion channels.
metal ion binding Binding to a metal ion.
mu-type opioid receptor binding Binding to a mu-type opioid receptor.
protein domain specific binding Binding to a specific domain of a protein.

38 GO annotations of biological process

Name Definition
adenylate cyclase-activating adrenergic receptor signaling pathway An adenylate cyclase-activating G protein-coupled receptor signaling pathway initiated by a ligand binding to an adrenergic receptor on the surface of the target cell, and ending with the regulation of a downstream cellular process.
adenylate cyclase-activating dopamine receptor signaling pathway An adenylate cyclase-activating G protein-coupled receptor signaling pathway initiated by dopamine binding to its receptor, and ending with the regulation of a downstream cellular process.
adenylate cyclase-activating G protein-coupled receptor signaling pathway A G protein-coupled receptor signaling pathway in which the signal is transmitted via the activation of adenylyl cyclase activity and a subsequent increase in the intracellular concentration of cyclic AMP (cAMP).
bone development The process whose specific outcome is the progression of bone over time, from its formation to the mature structure. Bone is the hard skeletal connective tissue consisting of both mineral and cellular components.
cartilage development The process whose specific outcome is the progression of a cartilage element over time, from its formation to the mature structure. Cartilage elements are skeletal elements that consist of connective tissue dominated by extracellular matrix containing collagen type II and large amounts of proteoglycan, particularly chondroitin sulfate.
cellular response to glucagon stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a glucagon stimulus.
cognition The operation of the mind by which an organism becomes aware of objects of thought or perception; it includes the mental activities associated with thinking, learning, and memory.
developmental growth The increase in size or mass of an entire organism, a part of an organism or a cell, where the increase in size or mass has the specific outcome of the progression of the organism over time from one condition to another.
embryonic cranial skeleton morphogenesis The process in which the anatomical structures of the cranial skeleton are generated and organized during the embryonic phase.
embryonic hindlimb morphogenesis The process, occurring in the embryo, by which the anatomical structures of the hindlimbs are generated and organized. The hindlimbs are the posterior limbs of an animal.
endochondral ossification Replacement ossification wherein bone tissue replaces cartilage.
energy reserve metabolic process The chemical reactions and pathways by which a cell derives energy from stored compounds such as fats or glycogen.
G protein-coupled receptor signaling pathway The series of molecular signals initiated by a ligand binding to its receptor, in which the activated receptor promotes the exchange of GDP for GTP on the alpha-subunit of an associated heterotrimeric G-protein complex. The GTP-bound activated alpha-G-protein then dissociates from the beta- and gamma-subunits to further transmit the signal within the cell. The pathway begins with receptor-ligand interaction, and ends with regulation of a downstream cellular process. The pathway can start from the plasma membrane, Golgi or nuclear membrane.
genomic imprinting The establishment of epigenetic modifications (imprints) during gametogenesis, leading to an asymmetry between the maternal and paternal alleles, leading to differential expression of the corresponding alleles. This can happen through heterochromatin formation or through differential chromatin loop formation.
hair follicle placode formation The developmental process in which a hair placode forms. An hair follicle placode is a thickening of the ectoderm that will give rise to the hair follicle bud.
multicellular organism growth The increase in size or mass of an entire multicellular organism, as opposed to cell growth.
negative regulation of blood pressure Any process in which the force of blood traveling through the circulatory system is decreased.
negative regulation of multicellular organism growth Any process that stops, prevents, or reduces the frequency, rate or extent of growth of an organism to reach its usual body size.
platelet aggregation The adhesion of one platelet to one or more other platelets via adhesion molecules.
positive regulation of cAMP-mediated signaling Any process which activates, maintains or increases the frequency, rate or extent of cAMP-mediated signaling.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
positive regulation of cold-induced thermogenesis Any process that activates or increases the frequency, rate or extent of cold-induced thermogenesis.
positive regulation of insulin secretion Any process that activates or increases the frequency, rate or extent of the regulated release of insulin.
positive regulation of osteoblast differentiation Any process that activates or increases the frequency, rate or extent of osteoblast differentiation.
positive regulation of osteoclast differentiation Any process that activates or increases the frequency, rate or extent of osteoclast differentiation.
positive regulation of protein phosphorylation Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein.
positive regulation of sodium ion transport Any process that increases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
post-embryonic body morphogenesis The process in which the anatomical structures of the post-embryonic soma are generated and organized.
post-embryonic development The process whose specific outcome is the progression of the organism over time, from the completion of embryonic development to the mature structure. See embryonic development.
regulation of parathyroid hormone secretion Any process that modulates the frequency, rate or extent of parathyroid hormone secretion.
regulation of signal transduction Any process that modulates the frequency, rate or extent of signal transduction.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to parathyroid hormone Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a parathyroid hormone stimulus.
response to xenobiotic stimulus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a xenobiotic, a compound foreign to the organim exposed to it. It may be synthesized by another organism (like ampicilin) or it can be a synthetic chemical.
sensory perception of chemical stimulus The series of events required for an organism to receive a sensory chemical stimulus, convert it to a molecular signal, and recognize and characterize the signal. This is a neurological process.
skeletal system development The process whose specific outcome is the progression of the skeleton over time, from its formation to the mature structure. The skeleton is the bony framework of the body in vertebrates (endoskeleton) or the hard outer envelope of insects (exoskeleton or dermoskeleton).
skin development The process whose specific outcome is the progression of the skin over time, from its formation to the mature structure. The skin is the external membranous integument of an animal. In vertebrates the skin generally consists of two layers, an outer nonsensitive and nonvascular epidermis (cuticle or skarfskin) composed of cells which are constantly growing and multiplying in the deeper, and being thrown off in the superficial layers, as well as an inner vascular dermis (cutis, corium or true skin) composed mostly of connective tissue.
tissue homeostasis A homeostatic process involved in the maintenance of an internal steady state within a defined tissue of an organism, including control of cellular proliferation and death and control of metabolic function.

23 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P04896 GNAS Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Bos taurus (Bovine) PR
P63091 GNAS Guanine nucleotide-binding protein G(s) subunit alpha Canis lupus familiaris (Dog) (Canis familiaris) PR
P38405 GNAL Guanine nucleotide-binding protein G Homo sapiens (Human) SS
P29797 GNAS Guanine nucleotide-binding protein G(s) subunit alpha Sus scrofa (Pig) PR
P93564 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum tuberosum (Potato) SS
P38406 Gnal Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q63210 Gna12 Guanine nucleotide-binding protein subunit alpha-12 Rattus norvegicus (Rat) SS
P19627 Gnaz Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P29348 Gnat3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
O88302 Gna15 Guanine nucleotide-binding protein subunit alpha-15 Rattus norvegicus (Rat) SS
Q9JID2 Gna11 Guanine nucleotide-binding protein subunit alpha-11 Rattus norvegicus (Rat) SS
P82471 Gnaq Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P04897 Gnai2 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P59215 Gnao1 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
P08753 Gnai3 Guanine nucleotide-binding protein G Rattus norvegicus (Rat) SS
Q6Q7Y5 Gna13 Guanine nucleotide-binding protein subunit alpha-13 Rattus norvegicus (Rat) SS
P10824 Gnai1 Guanine nucleotide-binding protein G subunit alpha-1 Rattus norvegicus (Rat) EV
Q0DJ33 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Oryza sativa subsp. japonica (Rice) SS
P49084 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Glycine max (Soybean) (Glycine hispida) SS
P93163 GPA2 Guanine nucleotide-binding protein alpha-2 subunit Glycine max (Soybean) (Glycine hispida) SS
O80462 XLG1 Extra-large guanine nucleotide-binding protein 1 Arabidopsis thaliana (Mouse-ear cress) PR
P18064 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Arabidopsis thaliana (Mouse-ear cress) SS
P26981 GPA1 Guanine nucleotide-binding protein alpha-1 subunit Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MGMLNCLHGN NMSGQHDIPP EVGDQPEQEP LEAQGAAAPG AGVGPAEEME TEPSNNEPIP
70 80 90 100 110 120
DETDSEVCGP PEDSKSDIQS PSQAFEEVQV GGDYSPPPEE AMPFEIQQPS LGDFWPTLEQ
130 140 150 160 170 180
PGPSGTPSGI KAFNPAILEP GTPTGAHPGL GAYSPPPEEA MPFEFNEPAQ EDRCQPPLQV
190 200 210 220 230 240
PDLAPGGPEA WVSRALPAEP GNLGFENTGF REDYSPPPEE SVPFQLDGEE FGGDSPPPGL
250 260 270 280 290 300
PRVTPQIGIG GEFPTVAVPS TLCLAPAANA PPLWVQGAIG RPFREAVRSP NFAYDISPME
310 320 330 340 350 360
ITRPLLEIGR ASTGVDDDTA VNMDSPPIAS DGPPIEVSGA PVKSEHAKRP PLERQAAETG
370 380 390 400 410 420
NSPISSTTAE EAKVPSLERG EGSPTQPETV HIKPAPVAES GTDSSKADPD SATHAVLQIG
430 440 450 460 470 480
PEEVGGVPTM PTDLPPASED AGPDVRAEPD GGTAPATPAE SEDNREPAAA AAAEPAAEPA
490 500 510 520 530 540
AEPAAEPAAE PAAEPAAEAV PDTEAESASG AVPDTQEEPA AAAASATPAE PAARAAPVTP
550 560 570 580 590 600
TEPATRAVPS ARAHPAAGAV PGASAMSAAA RAAAARAAYA GPLVWGARSL SATPAARASL
610 620 630 640 650 660
PARAAAAARA ASAARAVAAG RSASAAPSRA HLRPPSPEIQ VADPPTPRPA PRPSAWPDKY
670 680 690 700 710 720
ERGRSCCRYE AASGICEIES SSDESEEGAT GCFQWLLRRN RRPGQPRSHT VGSNPVRNFF
730 740 750 760 770 780
ARAFGSCFGL SECTRSRSLS PGKAKDPMEE RRKQMRKEAM EMREQKRADK KRSKLIDKQL
790 800 810 820 830 840
EEEKMDYMCT HRLLLLGAGE SGKSTIVKQM RILHVNGFNG EGGEEDPQAA RSNSDGEKAT
850 860 870 880 890 900
KVQDIKNNLK EAIETIVAAM SNLVPPVELA NPENQFRVDY ILSVMNVPNF DFPPEFYEHA
910 920 930 940 950 960
KALWEDEGVR ACYERSNEYQ LIDCAQYFLD KIDVIKQADY VPSDQDLPRC RVLTSGIFET
970 980 990 1000 1010 1020
KFQVDKVNFH MFDVGGQRDE RRKWIQCFND VTAIIFVVAS SSYNMVIRED NQTNRLQEAL
1030 1040 1050 1060 1070 1080
NLFKSIWNNR WLRTISVILF LNKQDLLAEK VLAGKSKIED YFPEFARYTT PEDATPEPGE
1090 1100 1110 1120 1130 1140
DPRVTRAKYF IRDEFLRIST ASGDGRHYCY PHFTCAVDTE NIRRVFNDCR DIIQRMHLRQ
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