Q63433

SS
Gene name
Pkn1 (Pkn, Prk1, Prkcl1)
Protein name
Serine/threonine-protein kinase N1
Names
EC 2.7.11.13 , Protease-activated kinase 1 , PAK-1 , Protein kinase C-like 1 , Protein kinase C-like PKN , Protein-kinase C-related kinase 1 , Serine-threonine protein kinase N
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:29355
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

Serine/threonine-protein kinase N1 (PKN1) is a PKC-related serine/threonine protein kinase involved in specific signal transduction responses such as regulation of intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion, and transcription regulation in cells. Its kinase activity is enhanced by fatty acids such as arachidonic acid or the small GTP-binding protein Rho. Residues 455-511 of PKN selectively inhibit the kinase activity of the catalytic fragment of PKN1. Arachidonic acid relieves the catalytic activity of wild-type PKN from autoinhibition by residues 455-511 of PKN.

Autoinhibitory domains (AIDs)

458-514 (autoinhibitory domain) SS

Target domain

619-878 (Protein kinase domain)

Relief mechanism

Ligand binding, PTM

Assay

Accessory elements

761-784 (Activation loop from InterPro)

Target domain

619-878 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q63433

Entry ID Method Resolution Chain Position Source
AF-Q63433-F1 Predicted AlphaFoldDB

3 variants for Q63433

Variant ID(s) Position Change Description Diseaes Association Provenance
rs8160378 650 L>V No EVA
rs8164149 712 H>N No EVA
rs3323410770 931 A>V No EVA

No associated diseases with Q63433

12 regional properties for Q63433

Type Name Position InterPro Accession
domain C2 domain 310 - 473 IPR000008
domain Protein kinase domain 619 - 878 IPR000719
domain AGC-kinase, C-terminal 879 - 946 IPR000961
active_site Serine/threonine-protein kinase, active site 740 - 752 IPR008271
domain HR1 rho-binding domain 25 - 101 IPR011072-1
domain HR1 rho-binding domain 114 - 194 IPR011072-2
domain HR1 rho-binding domain 202 - 284 IPR011072-3
binding_site Protein kinase, ATP binding site 625 - 648 IPR017441
domain Protein kinase, C-terminal 899 - 941 IPR017892
domain Serine/threonine-protein kinase N, first HR1 domain 32 - 98 IPR037313
domain Serine/threonine-protein kinase N1, second HR1 domain 121 - 192 IPR037317
domain Serine/threonine-protein kinase N, C2 domain 392 - 479 IPR037784

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Endosome
  • Cell membrane ; Peripheral membrane protein
  • Cleavage furrow
  • Midbody
  • Associates with chromatin in a ligand-dependent manner (By similarity)
  • Localization to endosomes is mediated via its interaction with RHOB
  • Accumulates during telophase at the cleavage furrow and finally concentrates around the midbody in cytokinesis (By similarity)
  • Association to the cell membrane is dependent on Ser-377 phosphorylation
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
cleavage furrow The cleavage furrow is a plasma membrane invagination at the cell division site. The cleavage furrow begins as a shallow groove and eventually deepens to divide the cytoplasm.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic vesicle A vesicle found in the cytoplasm of a cell.
endosome A vacuole to which materials ingested by endocytosis are delivered.
midbody A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

13 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
histone binding Binding to a histone, any of a group of water-soluble proteins found in association with the DNA of eukaryotic or archaeal chromosomes. They are involved in the condensation and coiling of chromosomes during cell division and have also been implicated in gene regulation and DNA replication. They may be chemically modified (methylated, acetlyated and others) to regulate gene transcription.
histone deacetylase binding Binding to histone deacetylase.
histone H3T11 kinase activity Catalysis of the reaction
nuclear androgen receptor binding Binding to a nuclear androgen receptor.
nuclear receptor coactivator activity A transcription coactivator activity that activates or increases the transcription of specific gene sets via binding to a DNA-bound nuclear receptor, either on its own or as part of a complex. Coactivators often act by altering chromatin structure and modifications. For example, one class of transcription coregulators modifies chromatin structure through covalent modification of histones. A second class remodels the conformation of chromatin in an ATP-dependent fashion. A third class modulates interactions of DNA-bound DNA-binding transcription factors with other transcription coregulators. A fourth class of coactivator activity is the bridging of a DNA-binding transcription factor to the general (basal) transcription machinery. The Mediator complex, which bridges sequence-specific DNA binding transcription factors and RNA polymerase, is also a transcription coactivator.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein kinase C binding Binding to protein kinase C.
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions
small GTPase binding Binding to a small monomeric GTPase.

15 GO annotations of biological process

Name Definition
B cell apoptotic process Any apoptotic process in a B cell, a lymphocyte of B lineage with the phenotype CD19-positive and capable of B cell mediated immunity.
B cell homeostasis The process of regulating the proliferation and elimination of B cells such that the total number of B cells within a whole or part of an organism is stable over time in the absence of an outside stimulus.
epithelial cell migration The orderly movement of an epithelial cell from one site to another, often during the development of a multicellular organism.
hyperosmotic response Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detection of, or exposure to, a hyperosmotic environment, i.e. an environment with a higher concentration of solutes than the organism or cell.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of B cell proliferation Any process that stops, prevents or reduces the rate or extent of B cell proliferation.
negative regulation of protein phosphorylation Any process that stops, prevents or reduces the rate of addition of phosphate groups to amino acids within a protein.
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.
post-translational protein modification The process of covalently altering one or more amino acids in a protein after the protein has been completely translated and released from the ribosome.
regulation of cell motility Any process that modulates the frequency, rate or extent of cell motility.
regulation of germinal center formation Any process that modulates the frequency, rate, or extent of germinal center formation.
regulation of immunoglobulin production Any process that modulates the frequency, rate, or extent of immunoglobulin production.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
renal system process A organ system process carried out by any of the organs or tissues of the renal system. The renal system maintains fluid balance, and contributes to electrolyte balance, acid/base balance, and disposal of nitrogenous waste products. In humans, the renal system comprises a pair of kidneys, a pair of ureters, urinary bladder, urethra, sphincter muscle and associated blood vessels; in other species, the renal system may comprise related structures (e.g., nephrocytes and malpighian tubules in Drosophila).
spleen development The process whose specific outcome is the progression of the spleen over time, from its formation to the mature structure. The spleen is a large vascular lymphatic organ composed of white and red pulp, involved both in hemopoietic and immune system functions.

34 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
A1A4I4 PKN1 Serine/threonine-protein kinase N1 Bos taurus (Bovine) SS
P83099 Pkcdelta Putative protein kinase C delta type homolog Drosophila melanogaster (Fruit fly) PR
A1Z7T0 Pkn Serine/threonine-protein kinase N Drosophila melanogaster (Fruit fly) SS
Q04759 PRKCQ Protein kinase C theta type Homo sapiens (Human) PR
Q6P5Z2 PKN3 Serine/threonine-protein kinase N3 Homo sapiens (Human) SS
Q96LW2 RSKR Ribosomal protein S6 kinase-related protein Homo sapiens (Human) PR
Q02156 PRKCE Protein kinase C epsilon type Homo sapiens (Human) SS
P24723 PRKCH Protein kinase C eta type Homo sapiens (Human) SS
Q16513 PKN2 Serine/threonine-protein kinase N2 Homo sapiens (Human) EV
Q05655 PRKCD Protein kinase C delta type Homo sapiens (Human) SS
Q16512 PKN1 Serine/threonine-protein kinase N1 Homo sapiens (Human) EV
P23298 Prkch Protein kinase C eta type Mus musculus (Mouse) PR
Q02111 Prkcq Protein kinase C theta type Mus musculus (Mouse) PR
Q8BWW9 Pkn2 Serine/threonine-protein kinase N2 Mus musculus (Mouse) SS
Q8K045 Pkn3 Serine/threonine-protein kinase N3 Mus musculus (Mouse) SS
P16054 Prkce Protein kinase C epsilon type Mus musculus (Mouse) PR
P28867 Prkcd Protein kinase C delta type Mus musculus (Mouse) PR
P70268 Pkn1 Serine/threonine-protein kinase N1 Mus musculus (Mouse) SS
Q64617 Prkch Protein kinase C eta type Rattus norvegicus (Rat) PR
O55173 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Rattus norvegicus (Rat) SS
P09217 Prkcz Protein kinase C zeta type Rattus norvegicus (Rat) SS
F1M7Y5 Prkci Protein kinase C iota type Rattus norvegicus (Rat) SS
P09215 Prkcd Protein kinase C delta type Rattus norvegicus (Rat) PR
P63319 Prkcg Protein kinase C gamma type Rattus norvegicus (Rat) SS
P47197 Akt2 RAC-beta serine/threonine-protein kinase Rattus norvegicus (Rat) SS
P47196 Akt1 RAC-alpha serine/threonine-protein kinase Rattus norvegicus (Rat) PR
Q63484 Akt3 RAC-gamma serine/threonine-protein kinase Rattus norvegicus (Rat) PR
O08874 Pkn2 Serine/threonine-protein kinase N2 Rattus norvegicus (Rat) SS
P09216 Prkce Protein kinase C epsilon type Rattus norvegicus (Rat) PR
P05696 Prkca Protein kinase C alpha type Rattus norvegicus (Rat) SS
P68403 Prkcb Protein kinase C beta type Rattus norvegicus (Rat) SS
P34722 tpa-1 Protein kinase C-like 1 Caenorhabditis elegans PR
A7MBL8 pkn2 Serine/threonine-protein kinase N2 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MAGDAVQSEP RSWSLLEQLG LAGADLAAPG VQQQLELERE RLKREIRKEL KLKEGAENLR
70 80 90 100 110 120
RATTDLGRSL APVELLLRGS ARRLDLLHQQ LQELHAHVVL PDPTAGSDAP QSLAEGSPVC
130 140 150 160 170 180
SSTNLSRVAG LEKQLAIELK VKQGAENMIQ TYSNGSTKDR KLLLTAQQML QDSKTKIDII
190 200 210 220 230 240
RMQLRRALQA LQAGQLESQA APDEAHGDPD LGAVELRIEE LRHHFRVEHA VAEGAKNVLR
250 260 270 280 290 300
LLSAAKAPDR KAVSEAQEKL TESNQKLGLL RESLERRLGE LPADHPKGRL LREELTAASS
310 320 330 340 350 360
AAFSAILPGP FPATHYSTLS KPAPLTGTLE VRVVGCKNLP ETIPWSPPPS VGASGTPDSR
370 380 390 400 410 420
TPFLSRPARG LYNRSGSLSG RSSLKGEAEN STEVSTVLKL DNTVVGQTAW KPCGPNAWDQ
430 440 450 460 470 480
SFTLELERAR ELELAVFWRD QRGLCALKFL KLEDFLDNER HEVQLDMEPQ GCLVAEVTFR
490 500 510 520 530 540
NPIIERIPRL QRQKKIFSKQ QGQTFQRARQ MNIDVATWVR LLRRLIPNAV ATGSFSPNAS
550 560 570 580 590 600
PGSEIRSTGD ISMEKLNLGA DSDSSSQKSP AGLPSTSCSL SSPTHESTTS PELPSETQET
610 620 630 640 650 660
PGPGLCSPLR KSPLTLEDFK FLAVLGRGHF GKVLLSEFHS SGELFAIKAL KKGDIVARDE
670 680 690 700 710 720
VESLMCEKRI LATVTRAGHP FLVNLFGCFQ TPEHVCFVME YSAGGDLMLH IHSDVFSEPR
730 740 750 760 770 780
AVFYSACVVL GLQFLHEHKI VYRDLKLDNL LLDTEGYVKI ADFGLCKEGM GYGDRTSTFC
790 800 810 820 830 840
GTPEFLAPEV LTDTSYTRAV DWWGLGVLLY EMLVGESPFP GDDEEEVFDS IVNDEVRYPR
850 860 870 880 890 900
FLSAEAIGIM RRLLRRNPER RLGSTERDAE DVKKQPFFRT LDWDALLARR LPPPFVPTLS
910 920 930 940
GRTDVSNFDE EFTGEAPTLS PPRDARPLTA AEQAAFRDFD FVAGGY