Descriptions
MCF2 protein is the prototype member of a large family of guanine nucleotide exchange factors (GEFs) that modulates the Rho family of GTPases. Removal of the N-terminal region results in a significant increase of MCF2 activity. Specifically, the autoinhibitory region binds to the PH domain, and limits the access of Rho GTPases to the catalytic DH domain and masks the intracellular targeting function of the PH domain.
Autoinhibitory domains (AIDs)
Target domain |
824-948 (PH domain) |
Relief mechanism |
Cleavage |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q63406
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q63406-F1 | Predicted | AlphaFoldDB |
No variants for Q63406
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q63406 | |||||
No associated diseases with Q63406
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| extrinsic component of membrane | The component of a membrane consisting of gene products and protein complexes that are loosely bound to one of its surfaces, but not integrated into the hydrophobic region. |
| lamellipodium | A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments. |
| membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. |
| plasma membrane | The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| 1-phosphatidylinositol binding | Binding to a phosphatidylinositol, a glycophospholipid with its sn-glycerol 3-phosphate residue is esterified to the 1-hydroxyl group of 1D-myo-inositol. |
| guanyl-nucleotide exchange factor activity | Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions. |
| phosphatidylinositol binding | Binding to an inositol-containing glycerophospholipid, i.e. phosphatidylinositol (PtdIns) and its phosphorylated derivatives. |
4 GO annotations of biological process
| Name | Definition |
|---|---|
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| positive regulation of Rho protein signal transduction | Any process that activates or increases the frequency, rate or extent of Rho protein signal transduction. |
| positive regulation of transcription by RNA polymerase II | Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter. |
| Rho protein signal transduction | The series of molecular signals within the cell that are mediated by a member of the Rho family of proteins switching to a GTP-bound active state. |
12 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| O60229 | KALRN | Kalirin | Homo sapiens (Human) | SS |
| Q86YR7 | MCF2L2 | Probable guanine nucleotide exchange factor MCF2L2 | Homo sapiens (Human) | SS |
| O75962 | TRIO | Triple functional domain protein | Homo sapiens (Human) | EV |
| P10911 | MCF2 | Proto-oncogene DBL | Homo sapiens (Human) | EV |
| O15068 | MCF2L | Guanine nucleotide exchange factor DBS | Homo sapiens (Human) | SS |
| Q0KL02 | Trio | Triple functional domain protein | Mus musculus (Mouse) | SS |
| A2CG49 | Kalrn | Kalirin | Mus musculus (Mouse) | SS |
| Q64096 | Mcf2l | Guanine nucleotide exchange factor DBS | Mus musculus (Mouse) | SS |
| P97924 | Kalrn | Kalirin | Rattus norvegicus (Rat) | SS |
| Q6P720 | Arhgef25 | Rho guanine nucleotide exchange factor 25 | Rattus norvegicus (Rat) | SS |
| F1M0Z1 | Trio | Triple functional domain protein | Rattus norvegicus (Rat) | SS |
| Q1LUA6 | trio | Triple functional domain protein | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSNCWCFIFC | KERVRSNSSS | PQHDGTSREE | ADHQVDVSDG | IRLVPDKAEA | TMATASDEIM |
| 70 | 80 | 90 | 100 | 110 | 120 |
| HQDIVPLCAA | DIQEQLKKRF | AYLSGGRGQD | GSPVITFPDY | PAFSEIPDKE | FQNVMTYLTS |
| 130 | 140 | 150 | 160 | 170 | 180 |
| IPSLQDAGIG | FILVIDRRQD | KWTSVKASVL | RIAASFPANL | QLVLVLRPTG | FFQRTLSDIA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| FKFNRDEFKM | KVPVMMLSSV | PELHGYIDKS | QLTEDLGGTL | DYCHSRWLCH | RTAIESFALM |
| 250 | 260 | 270 | 280 | 290 | 300 |
| VKQTAQMLQA | FGTELAETEL | PNDVQSTSLV | LSAHTEKKAK | VKEDLQLALT | EGNSILESLR |
| 310 | 320 | 330 | 340 | 350 | 360 |
| EPLAESIVHS | VNQDQLDNQA | TVKRLLTQLN | ETEAAFDEFW | AKHQQKLEQC | LQLRHFEQGF |
| 370 | 380 | 390 | 400 | 410 | 420 |
| REVKTALDSM | SQKIAAFTDV | GNSLAHVQHL | LKDLTTFEEK | SSVAVDKARA | LSLEGQQLIE |
| 430 | 440 | 450 | 460 | 470 | 480 |
| NRHYAVDSIH | PKCEELQHLC | DHFASEVTRR | RDLLSKSLEL | HSLLETSMKW | SDEGIFLLAS |
| 490 | 500 | 510 | 520 | 530 | 540 |
| QPVDKCQSQD | GAEAALQEIE | KFLETGAENK | IQELNKIYKE | YECILNQDLL | EHVQKVFQKQ |
| 550 | 560 | 570 | 580 | 590 | 600 |
| ESTEEMFHRR | QASLKKLAAK | QTRPVQPVAP | RPEALTKSPS | PSPGSWRSSE | NSSSEGNALR |
| 610 | 620 | 630 | 640 | 650 | 660 |
| RGPYRRAKSE | MSEPRQGRTS | STGEEEESLA | ILRRHVMNEL | LDTERAYVEE | LLCVLEGYAA |
| 670 | 680 | 690 | 700 | 710 | 720 |
| EMDNPLMAHL | ISTGLQNKKN | ILFGNMEEIY | HFHNRIFLRE | LESCIDCPEL | VGRCFLERME |
| 730 | 740 | 750 | 760 | 770 | 780 |
| EFQIYEKYCQ | NKPRSESLWR | QCSDCPFFQE | CQKKLDHKLS | LDSYLLKPVQ | RITKYQLLLK |
| 790 | 800 | 810 | 820 | 830 | 840 |
| EMLKYSKHCE | GAEDLQEALS | SILGILKAVN | DSMHLIAITG | YDGNLGDLGK | LLMQGSFSVW |
| 850 | 860 | 870 | 880 | 890 | 900 |
| TDHKKGHTKV | KELARFKPMQ | RHLFLHEKAV | LFCKKREENG | EGYEKAPSYS | YKQSLNMTAV |
| 910 | 920 | 930 | 940 | 950 | 960 |
| GITENVKGDT | KKFEIWYNAR | EEVYIIQAPT | PEIKAAWVNE | IRKVLTSQLQ | ACREASQHRA |
| 970 | 980 | 990 | 1000 | 1010 | 1020 |
| LEQSHSLPLP | TPASTSPTKG | STRNVKKLED | RKTDPLCLEG | CVSSSLPKPP | EKGKGWSKTS |
| 1030 | 1040 | 1050 | 1060 | 1070 | 1080 |
| HSLEAPEEDG | GWSSAEELIN | SSDAEEDGGV | GPRKLVPGKY | TVLMDGEKGG | SDTLAMRSGD |
| 1090 | 1100 | 1110 | 1120 | 1130 | 1140 |
| MVEVVEEGTE | GLWYVRDLTS | SKEGWVPASS | LATLLGKSSS | AQCLSSSGKT | HCARQLCPEP |
| AKILSPEPV |