Q62167

SS
Gene name
Ddx3x
Protein name
ATP-dependent RNA helicase DDX3X
Names
D1Pas1-related sequence 2, DEAD box RNA helicase DEAD3, mDEAD3, DEAD box protein 3, X-chromosomal, Embryonic RNA helicase
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:13205
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

DEAD-box proteins utilize ATP to bind and remodel RNA and RNA-protein complexes. The Ded1/DDX3 subfamily of DEAD-box proteins is of particular interest as their function during protein translation, are essential for viability, and are frequently altered in human malignancies. <br>There is a unique interdomain interaction between the two ATPase domains (DEAD box helicase domain and helicase domain) in which the C-terminal helicase domain clashes with the RNA-binding surface. Destabilizing this interaction accelerates RNA duplex unwinding, suggesting that it is present in solution and inhibitory for catalysis. The N-terminal extension to the DEAD box helicase interacts with DEAD box helicase domain and C-terminal helicase domain, and stabilizes the autoinhibited state of DDX3X.

Autoinhibitory domains (AIDs)

132-182 (N-terminal extension) SS

Target domain

204-391 (DEAD box helicase domain); 414-575 (C-terminal helicase domain)

Relief mechanism

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q62167

Entry ID Method Resolution Chain Position Source
AF-Q62167-F1 Predicted AlphaFoldDB

2 variants for Q62167

Variant ID(s) Position Change Description Diseaes Association Provenance
rs583709872 547 E>D No Ensembl
rs586229297 550 I>V No Ensembl

No associated diseases with Q62167

5 regional properties for Q62167

Type Name Position InterPro Accession
conserved_site ATP-dependent RNA helicase DEAD-box, conserved site 345 - 353 IPR000629
domain Helicase, C-terminal 414 - 575 IPR001650
domain DEAD/DEAH box helicase domain 204 - 391 IPR011545
domain Helicase superfamily 1/2, ATP-binding domain 199 - 418 IPR014001
domain RNA helicase, DEAD-box type, Q motif 180 - 208 IPR014014

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Cell membrane
  • Nucleus
  • Cytoplasm
  • Cytoplasm, Stress granule
  • Inflammasome
  • Cell projection, lamellipodium
  • Shuttles between the nucleus and the cytosol
  • Exported from the nucleus partly through the XPO1/CRM1 system and partly through NXF1/TAP
  • Localizes to nuclear pores on the outer side of the nuclear membrane
  • In the cytosol, partly colocalizes with mitochondria
  • At G0, predominantly located in nucleus
  • In G1/S phase, predominantly cytoplasmic
  • During prophase/prometaphase, localizes in close proximity to the condensing chromosomes
  • During telophase, localizes around the newly synthesized nuclear membrane and in the cytoplasm
  • Colocalizes with TRPV4 at the plasma membrane
  • When TRPV4 channel is activated, intracellular Ca(2+) levels increase and the calmodulin/CAMKII pathway is activated, relocalizes to the nucleus
  • WNT3A stimulation promotes DDX3 recruitment to the plasma membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

13 GO annotations of cellular component

Name Definition
cell leading edge The area of a motile cell closest to the direction of movement.
centrosome A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytoplasmic stress granule A dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
cytosolic small ribosomal subunit The small subunit of a ribosome located in the cytosol.
eukaryotic translation initiation factor 3 complex A complex of several polypeptides that plays at least two important roles in protein synthesis: First, eIF3 binds to the 40S ribosome and facilitates loading of the Met-tRNA/eIF2.GTP ternary complex to form the 43S preinitiation complex. Subsequently, eIF3 apparently assists eIF4 in recruiting mRNAs to the 43S complex. The eIF3 complex contains five conserved core subunits, and may contain several additional proteins; the non-core subunits are thought to mediate association of the complex with specific sets of mRNAs.
lamellipodium A thin sheetlike process extended by the leading edge of a migrating cell or extending cell process; contains a dense meshwork of actin filaments.
NLRP3 inflammasome complex An inflammasome complex that consists of three components, NLRP3 (NALP3), PYCARD and caspase-1. It is activated upon exposure to whole pathogens, as well as a number of structurally diverse pathogen- and danger-associated molecular patterns (PAMPs and DAMPs) and environmental irritants. Whole pathogens demonstrated to activate the NLRP3 inflammasome complex include the fungi Candida albicans and Saccharomyces cerevisiae, bacteria that produce pore-forming toxins, including Listeria monocytogenes and Staphylococcus aureus, and viruses such as Sendai virus, adenovirus, and influenza virus.
nucleoplasm That part of the nuclear content other than the chromosomes or the nucleolus.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
P granule A small cytoplasmic, non-membranous RNA/protein complex aggregate in the primordial germ cells of many higher eukaryotes.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

20 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
CTPase activity Catalysis of the reaction: CTP + H2O = CDP + H+ + phosphate. May or may not be coupled to another reaction.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA helicase activity Unwinding of a DNA helix, driven by ATP hydrolysis.
eukaryotic initiation factor 4E binding Binding to eukaryotic initiation factor 4E, a polypeptide factor involved in the initiation of ribosome-mediated translation.
gamma-tubulin binding Binding to the microtubule constituent protein gamma-tubulin.
GTPase activity Catalysis of the reaction: GTP + H2O = GDP + H+ + phosphate.
mRNA 5'-UTR binding Binding to an mRNA molecule at its 5' untranslated region.
mRNA binding Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.
nucleoside-triphosphatase activity Catalysis of the reaction: a ribonucleoside triphosphate + H2O = a ribonucleoside diphosphate + H+ + phosphate.
poly(A) binding Binding to a sequence of adenylyl residues in an RNA molecule, such as the poly(A) tail, a sequence of adenylyl residues at the 3' end of eukaryotic mRNA.
protein serine/threonine kinase activator activity Binds to and increases the activity of a protein serine/threonine kinase.
ribosomal small subunit binding Binding to a small ribosomal subunit.
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.
RNA stem-loop binding Binding to a stem-loop in an RNA molecule. An RNA stem-loop is a secondary RNA structure consisting of a double-stranded RNA (dsRNA) stem and a terminal loop.
RNA strand annealing activity An activity that facilitates the formation of a complementary double-stranded RNA molecule.
transcription factor binding Binding to a transcription factor, a protein required to initiate or regulate transcription.
translation initiation factor binding Binding to a translation initiation factor, any polypeptide factor involved in the initiation of ribosome-mediated translation.

49 GO annotations of biological process

Name Definition
cell differentiation The process in which relatively unspecialized cells, e.g. embryonic or regenerative cells, acquire specialized structural and/or functional features that characterize the cells, tissues, or organs of the mature organism or some other relatively stable phase of the organism's life history. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state.
cellular response to arsenic-containing substance Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an arsenic stimulus from compounds containing arsenic, including arsenates, arsenites, and arsenides.
cellular response to osmotic stress Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating an increase or decrease in the concentration of solutes outside the organism or cell.
cellular response to virus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a virus.
chromosome segregation The process in which genetic material, in the form of chromosomes, is organized into specific structures and then physically separated and apportioned to two or more sets. In eukaryotes, chromosome segregation begins with the condensation of chromosomes, includes chromosome separation, and ends when chromosomes have completed movement to the spindle poles.
extrinsic apoptotic signaling pathway via death domain receptors The series of molecular signals in which a signal is conveyed from the cell surface to trigger the apoptotic death of a cell. The pathway starts with a ligand binding to a death domain receptor on the cell surface, and ends when the execution phase of apoptosis is triggered.
gamete generation The generation and maintenance of gametes in a multicellular organism. A gamete is a haploid reproductive cell.
inflammatory response The immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents. The process is characterized by local vasodilation, extravasation of plasma into intercellular spaces and accumulation of white blood cells and macrophages.
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
intrinsic apoptotic signaling pathway The series of molecular signals in which an intracellular signal is conveyed to trigger the apoptotic death of a cell. The pathway starts with reception of an intracellular signal (e.g. DNA damage, endoplasmic reticulum stress, oxidative stress etc.), and ends when the execution phase of apoptosis is triggered. The intrinsic apoptotic signaling pathway is crucially regulated by permeabilization of the mitochondrial outer membrane (MOMP).
lipid homeostasis Any process involved in the maintenance of an internal steady state of lipid within an organism or cell.
mature ribosome assembly The aggregation, arrangement and bonding together of the large and small ribosomal subunits into a functional ribosome.
negative regulation of apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process.
negative regulation of cell growth Any process that stops, prevents, or reduces the frequency, rate, extent or direction of cell growth.
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of a cysteine-type endopeptidase activity involved in the apoptotic process.
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Any process that stops, prevents or reduces the frequency, rate or extent of extrinsic apoptotic signaling pathway via death domain receptors.
negative regulation of intrinsic apoptotic signaling pathway Any process that stops, prevents or reduces the frequency, rate or extent of intrinsic apoptotic signaling pathway.
negative regulation of NIK/NF-kappaB signaling Any process that stops, prevents or reduces the frequency, rate or extent of NIK/NF-kappaB signaling.
negative regulation of protein-containing complex assembly Any process that stops, prevents, or reduces the frequency, rate or extent of protein complex assembly.
negative regulation of translation Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.
positive regulation of apoptotic process Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process.
positive regulation of canonical Wnt signaling pathway Any process that increases the rate, frequency, or extent of the Wnt signaling pathway through beta-catenin, the series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell, followed by propagation of the signal via beta-catenin, and ending with a change in transcription of target genes.
positive regulation of cell growth Any process that activates or increases the frequency, rate, extent or direction of cell growth.
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Any process that activates or increases the activity of a cysteine-type endopeptidase involved in the apoptotic process.
positive regulation of G1/S transition of mitotic cell cycle Any signalling pathway that increases or activates a cell cycle cyclin-dependent protein kinase to modulate the switch from G1 phase to S phase of the mitotic cell cycle.
positive regulation of gene expression Any process that increases the frequency, rate or extent of gene expression. Gene expression is the process in which a gene's coding sequence is converted into a mature gene product (protein or RNA).
positive regulation of interferon-alpha production Any process that activates or increases the frequency, rate, or extent of interferon-alpha production.
positive regulation of interferon-beta production Any process that activates or increases the frequency, rate, or extent of interferon-beta production.
positive regulation of NIK/NF-kappaB signaling Any process that activates or increases the frequency, rate or extent of NIK/NF-kappaB signaling.
positive regulation of NLRP3 inflammasome complex assembly Any process that activates or increases the frequency, rate or extent of NLRP3 inflammasome complex assembly.
positive regulation of protein acetylation Any process that activates or increases the frequency, rate or extent of protein acetylation.
positive regulation of protein autophosphorylation Any process that activates or increases the frequency, rate or extent of the phosphorylation by a protein of one or more of its own residues.
positive regulation of protein K63-linked ubiquitination Any process that activates or increases the frequency, rate or extent of protein K63-linked ubiquitination.
positive regulation of protein serine/threonine kinase activity Any process that increases the rate, frequency, or extent of protein serine/threonine kinase activity.
positive regulation of toll-like receptor 7 signaling pathway Any process that activates or increases the frequency, rate, or extent of toll-like receptor 7 signaling pathway.
positive regulation of toll-like receptor 8 signaling pathway Any process that activates or increases the frequency, rate, or extent of toll-like receptor 8 signaling pathway.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
positive regulation of translation Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.
positive regulation of translation in response to endoplasmic reticulum stress Any process that activates, or increases the frequency, rate or extent of translation as a result of endoplasmic reticulum stress.
positive regulation of translational initiation Any process that activates or increases the frequency, rate or extent of translational initiation.
positive regulation of viral genome replication Any process that activates or increases the frequency, rate or extent of viral genome replication.
primary miRNA processing A process involved in the conversion of a primary microRNA transcript into a pre-microRNA molecule.
protein localization to cytoplasmic stress granule A process in which a protein is transported to, or maintained in, a location within a cytoplasmic stress granule.
response to virus Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a virus.
RNA secondary structure unwinding The process in which a secondary structure of RNA are broken or 'melted'.
stress granule assembly The aggregation, arrangement and bonding together of proteins and RNA molecules to form a stress granule.
translational initiation The process preceding formation of the peptide bond between the first two amino acids of a protein. This includes the formation of a complex of the ribosome, mRNA or circRNA, and an initiation complex that contains the first aminoacyl-tRNA.
Wnt signaling pathway The series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state.

28 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q5W5U4 DDX4 Probable ATP-dependent RNA helicase DDX4 Bos taurus (Bovine) SS
Q6GVM6 DDX3Y ATP-dependent RNA helicase DDX3Y Pan troglodytes (Chimpanzee) SS
Q9NQI0 DDX4 Probable ATP-dependent RNA helicase DDX4 Homo sapiens (Human) SS
P17844 DDX5 Probable ATP-dependent RNA helicase DDX5 Homo sapiens (Human) PR
O15523 DDX3Y ATP-dependent RNA helicase DDX3Y Homo sapiens (Human) SS
O00571 DDX3X ATP-dependent RNA helicase DDX3X Homo sapiens (Human) EV
P16381 D1Pas1 Putative ATP-dependent RNA helicase Pl10 Mus musculus (Mouse) SS
Q61496 Ddx4 ATP-dependent RNA helicase DDX4 Mus musculus (Mouse) SS
Q62095 Ddx3y ATP-dependent RNA helicase DDX3Y Mus musculus (Mouse) SS
Q99MJ9 Ddx50 ATP-dependent RNA helicase DDX50 Mus musculus (Mouse) PR
Q91VC3 Eif4a3 Eukaryotic initiation factor 4A-III Mus musculus (Mouse) PR
Q9Z1N5 Ddx39b Spliceosome RNA helicase Ddx39b Mus musculus (Mouse) PR
Q9QY15 Ddx25 ATP-dependent RNA helicase DDX25 Mus musculus (Mouse) PR
Q61655 Ddx19a ATP-dependent RNA helicase DDX19A Mus musculus (Mouse) SS
Q501J6 Ddx17 Probable ATP-dependent RNA helicase DDX17 Mus musculus (Mouse) PR
Q8VDW0 Ddx39a ATP-dependent RNA helicase DDX39A Mus musculus (Mouse) PR
Q91VN6 Ddx41 Probable ATP-dependent RNA helicase DDX41 Mus musculus (Mouse) PR
Q61656 Ddx5 Probable ATP-dependent RNA helicase DDX5 Mus musculus (Mouse) PR
Q6GWX0 DDX4 Probable ATP-dependent RNA helicase DDX4 Sus scrofa (Pig) SS
Q2R1M8 Os11g0599500 DEAD-box ATP-dependent RNA helicase 52C Oryza sativa subsp. japonica (Rice) SS
Q6Z4K6 PL10B DEAD-box ATP-dependent RNA helicase 52B Oryza sativa subsp. japonica (Rice) SS
Q75HJ0 PL10A DEAD-box ATP-dependent RNA helicase 37 Oryza sativa subsp. japonica (Rice) SS
O01836 glh-3 ATP-dependent RNA helicase glh-3 Caenorhabditis elegans PR
D0PV95 laf-1 ATP-dependent RNA helicase laf-1 Caenorhabditis elegans SS
Q84W89 RH37 DEAD-box ATP-dependent RNA helicase 37 Arabidopsis thaliana (Mouse-ear cress) SS
Q8LA13 RH11 DEAD-box ATP-dependent RNA helicase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2F9 RH52 DEAD-box ATP-dependent RNA helicase 52 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C718 RH20 DEAD-box ATP-dependent RNA helicase 20 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MSHVAVENAL GLDQQFAGLD LNSSDNQSGG STASKGRYIP PHLRNREATK GFYDKDSSGW
70 80 90 100 110 120
SSSKDKDAYS SFGSRGDSRG KSSFFGDRGS GSRGRFDDRG RGDYDGIGGR GDRSGFGKFE
130 140 150 160 170 180
RGGNSRWCDK SDEDDWSKPL PPSERLEQEL FSGGNTGINF EKYDDIPVEA TGNNCPPHIE
190 200 210 220 230 240
SFSDVEMGEI IMGNIELTRY TRPTPVQKHA IPIIKEKRDL MACAQTGSGK TAAFLLPILS
250 260 270 280 290 300
QIYADGPGEA LRAMKENGRY GRRKQYPISL VLAPTRELAV QIYEEARKFS YRSRVRPCVV
310 320 330 340 350 360
YGGAEIGQQI RDLERGCHLL VATPGRLVDM MERGKIGLDF CKYLVLDEAD RMLDMGFEPQ
370 380 390 400 410 420
IRRIVEQDTM PPKGVRHTMM FSATFPKEIQ MLARDFLDEY IFLAVGRVGS TSENITQKVV
430 440 450 460 470 480
WVEEIDKRSF LLDLLNATGK DSLTLVFVET KKGADSLEDF LYHEGYACTS IHGDRSQRDR
490 500 510 520 530 540
EEALHQFRSG KSPILVATAV AARGLDISNV KHVINFDLPS DIEEYVHRIG RTGRVGNLGL
550 560 570 580 590 600
ATSFFNERNI NITKDLLDLL VEAKQEVPSW LENMAFEHHY KGSSRGRSKS SRFSGGFGAR
610 620 630 640 650 660
DYRQSSGASS SSFSSSRASS SRSGGGGHGG SRGFGGGGYG GFYNSDGYGG NYNSQGVDWW
GN