Q62074

SS
Gene name
Prkci (Pkcl)
Protein name
Protein kinase C iota type
Names
EC 2.7.11.13 , Atypical protein kinase C-lambda/iota , aPKC-lambda/iota , nPKC-iota
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:18759
EC number
2.7.11.13: Protein-serine/threonine kinases
Protein Class

Descriptions

PRKCI, an atypical PKC (aPKC) isoform, is an important kinase in multiple cellular processes and regulated by the phosphatidylinositol 3-kinase product phosphatidylinositol 3,4,5-(PO4)3 (PIP3). In aPKC molecule, the arginine motif (123-140) in the pseudosubstrate region on the regulatory domain binds to acidic residues in the substrate-binding region on the catalytic domain, leading to the off state of aPKCs. The autoinhibition of aPKC is released by binding with the acidic ligand PIP3 and consequently exposing the substrate-binding site.

Autoinhibitory domains (AIDs)

123-140 (Pseudosubstrate region) SS

Target domain

232-595 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota)

Relief mechanism

Ligand binding

Assay

Accessory elements

394-417 (Activation loop from InterPro)

Target domain

232-595 (Catalytic domain of the Serine/Threonine Kinase, Atypical Protein Kinase C iota)

Relief mechanism

Assay

394-417 (Activation loop from InterPro)

Target domain

232-595 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

2 structures for Q62074

Entry ID Method Resolution Chain Position Source
4DC2 X-ray 240 A A 231-595 PDB
AF-Q62074-F1 Predicted AlphaFoldDB

11 variants for Q62074

Variant ID(s) Position Change Description Diseaes Association Provenance
rs3388622418 59 S>P No EVA
rs3388620499 59 S>Y No EVA
rs3392893913 157 C>G No EVA
rs3388627963 181 K>* No EVA
rs29846252 200 M>T No EVA
rs3392824043 316 G>F No EVA
rs3388625321 404 R>Q No EVA
rs3388628849 417 N>K No EVA
rs3388617992 422 E>K No EVA
rs3388622454 537 P>L No EVA
rs3388621501 556 T>N No EVA

No associated diseases with Q62074

14 regional properties for Q62074

Type Name Position InterPro Accession
domain PB1 domain 25 - 107 IPR000270
domain Protein kinase domain 253 - 521 IPR000719
domain AGC-kinase, C-terminal 522 - 593 IPR000961
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 140 - 192 IPR002219
active_site Serine/threonine-protein kinase, active site 373 - 385 IPR008271
binding_site Protein kinase, ATP binding site 259 - 286 IPR017441
domain Protein kinase, C-terminal 545 - 583 IPR017892
domain Diacylglycerol/phorbol-ester binding 138 - 152 IPR020454-1
domain Diacylglycerol/phorbol-ester binding 154 - 163 IPR020454-2
domain Diacylglycerol/phorbol-ester binding 167 - 178 IPR020454-3
domain Diacylglycerol/phorbol-ester binding 179 - 191 IPR020454-4
domain Atypical protein kinase C iota type, catalytic domain 232 - 595 IPR034661
domain Protein kinase C, PB1 domain 26 - 108 IPR034877
domain PB1-like domain 25 - 108 IPR053793

Functions

Description
EC Number 2.7.11.13 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Membrane
  • Endosome
  • Nucleus
  • Transported into the endosome through interaction with SQSTM1/p62
  • After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1
  • Colocalizes with CDK7 in the cytoplasm and nucleus
  • Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

16 GO annotations of cellular component

Name Definition
apical part of cell The region of a polarized cell that forms a tip or is distal to a base. For example, in a polarized epithelial cell, the apical region has an exposed surface and lies opposite to the basal lamina that separates the epithelium from other tissue.
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
bicellular tight junction An occluding cell-cell junction that is composed of a branching network of sealing strands that completely encircles the apical end of each cell in an epithelial sheet; the outer leaflets of the two interacting plasma membranes are seen to be tightly apposed where sealing strands are present. Each sealing strand is composed of a long row of transmembrane adhesion proteins embedded in each of the two interacting plasma membranes.
brush border The dense covering of microvilli on the apical surface of an epithelial cell in tissues such as the intestine, kidney, and choroid plexus; the microvilli aid absorption by increasing the surface area of the cell.
cell leading edge The area of a motile cell closest to the direction of movement.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
endosome A vacuole to which materials ingested by endocytosis are delivered.
glutamatergic synapse A synapse that uses glutamate as a neurotransmitter.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.
intercellular bridge A direct connection between the cytoplasm of two cells that is formed following the completion of cleavage furrow ingression during cell division. They are usually present only briefly prior to completion of cytokinesis. However, in some cases, such as the bridges between germ cells during their development, they become stabilised.
microtubule cytoskeleton The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
PAR polarity complex A protein kinase complex that is required for the establishment of a cell polarity axis during the cell division cycle. Binds directly to activated CDC42 GTPase and is required for orchestrating a cellular gradient of CDC42. In S. cerevisiae components are
Schaffer collateral - CA1 synapse A synapse between the Schaffer collateral axon of a CA3 pyramidal cell and a CA1 pyramidal cell.
Schmidt-Lanterman incisure Regions within compact myelin in which the cytoplasmic faces of the enveloping myelin sheath are not tightly juxtaposed, and include cytoplasm from the cell responsible for making the myelin. Schmidt-Lanterman incisures occur in the compact myelin internode, while lateral loops are analogous structures found in the paranodal region adjacent to the nodes of Ranvier.

7 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
diacylglycerol-dependent serine/threonine kinase activity Catalysis of the reaction
metal ion binding Binding to a metal ion.
phospholipid binding Binding to a phospholipid, a class of lipids containing phosphoric acid as a mono- or diester.
protein kinase activity Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction
protein serine kinase activity Catalysis of the reactions
protein serine/threonine kinase activity Catalysis of the reactions

21 GO annotations of biological process

Name Definition
actin filament organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of cytoskeletal structures comprising actin filaments. Includes processes that control the spatial distribution of actin filaments, such as organizing filaments into meshworks, bundles, or other structures, as by cross-linking.
cell migration The controlled self-propelled movement of a cell from one site to a destination guided by molecular cues.
cell-cell junction organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a cell-cell junction. A cell-cell junction is a specialized region of connection between two cells.
cellular response to insulin stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an insulin stimulus. Insulin is a polypeptide hormone produced by the islets of Langerhans of the pancreas in mammals, and by the homologous organs of other organisms.
establishment of apical/basal cell polarity The specification and formation of the polarity of a cell along its apical/basal axis.
establishment or maintenance of epithelial cell apical/basal polarity Any cellular process that results in the specification, formation or maintenance of the apicobasal polarity of an epithelial cell.
eye photoreceptor cell development Development of a photoreceptor, a sensory cell in the eye that reacts to the presence of light. They usually contain a pigment that undergoes a chemical change when light is absorbed, thus stimulating a nerve.
Golgi vesicle budding The evagination of the Golgi membrane, resulting in formation of a vesicle.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
negative regulation of glial cell apoptotic process Any process that stops, prevents, or reduces the frequency, rate, or extent of glial cell apoptotic process.
negative regulation of neuron apoptotic process Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process in neurons.
positive regulation of endothelial cell apoptotic process Any process that activates or increases the frequency, rate or extent of endothelial cell apoptotic process.
positive regulation of glial cell proliferation Any process that activates or increases the rate or extent of glial cell proliferation.
positive regulation of glucose import Any process that activates or increases the frequency, rate or extent of the import of the hexose monosaccharide glucose into a cell or organelle.
positive regulation of neuron projection development Any process that increases the rate, frequency or extent of neuron projection development. Neuron projection development is the process whose specific outcome is the progression of a neuron projection over time, from its formation to the mature structure. A neuron projection is any process extending from a neural cell, such as axons or dendrites (collectively called neurites).
positive regulation of NF-kappaB transcription factor activity Any process that activates or increases the frequency, rate or extent of activity of the transcription factor NF-kappaB.
positive regulation of protein localization to plasma membrane Any process that activates or increases the frequency, rate or extent of protein localization to plasma membrane.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of postsynaptic membrane neurotransmitter receptor levels Any process that regulates the the local concentration of neurotransmitter receptor at the postsynaptic membrane.
response to interleukin-1 Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-1 stimulus.
response to peptide hormone Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a peptide hormone stimulus. A peptide hormone is any of a class of peptides that are secreted into the blood stream and have endocrine functions in living animals.

27 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P24583 PKC1 Protein kinase C-like 1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
A1Z9X0 aPKC Atypical protein kinase C Drosophila melanogaster (Fruit fly) SS
Q05513 PRKCZ Protein kinase C zeta type Homo sapiens (Human) SS
P41743 PRKCI Protein kinase C iota type Homo sapiens (Human) EV
P31750 Akt1 RAC-alpha serine/threonine-protein kinase Mus musculus (Mouse) PR
Q60823 Akt2 RAC-beta serine/threonine-protein kinase Mus musculus (Mouse) PR
Q9Z2A0 Pdpk1 3-phosphoinositide-dependent protein kinase 1 Mus musculus (Mouse) SS
Q02956 Prkcz Protein kinase C zeta type Mus musculus (Mouse) SS
P70268 Pkn1 Serine/threonine-protein kinase N1 Mus musculus (Mouse) SS
Q8K045 Pkn3 Serine/threonine-protein kinase N3 Mus musculus (Mouse) SS
Q8BWW9 Pkn2 Serine/threonine-protein kinase N2 Mus musculus (Mouse) SS
P23298 Prkch Protein kinase C eta type Mus musculus (Mouse) PR
P16054 Prkce Protein kinase C epsilon type Mus musculus (Mouse) PR
Q02111 Prkcq Protein kinase C theta type Mus musculus (Mouse) PR
P28867 Prkcd Protein kinase C delta type Mus musculus (Mouse) PR
P20444 Prkca Protein kinase C alpha type Mus musculus (Mouse) SS
P63318 Prkcg Protein kinase C gamma type Mus musculus (Mouse) SS
P68404 Prkcb Protein kinase C beta type Mus musculus (Mouse) SS
Q9WUA6 Akt3 RAC-gamma serine/threonine-protein kinase Mus musculus (Mouse) PR
Q9ERE3 Sgk3 Serine/threonine-protein kinase Sgk3 Mus musculus (Mouse) PR
Q8QZV4 Stk32c Serine/threonine-protein kinase 32C Mus musculus (Mouse) PR
Q91VJ4 Stk38 Serine/threonine-protein kinase 38 Mus musculus (Mouse) SS
Q7TSE6 Stk38l Serine/threonine-protein kinase 38-like Mus musculus (Mouse) SS
P09217 Prkcz Protein kinase C zeta type Rattus norvegicus (Rat) SS
F1M7Y5 Prkci Protein kinase C iota type Rattus norvegicus (Rat) SS
Q19266 pkc-3 Protein kinase C-like 3 Caenorhabditis elegans SS
Q90XF2 prkci Protein kinase C iota type Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MPTQRDSSTM SHTVACGGGG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CSEVRDMCSF
70 80 90 100 110 120
DNEQPFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE
130 140 150 160 170 180
DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK
190 200 210 220 230 240
KCHKLVTIEC GRHSLPPEPM MPMDQTMHPD HTQTVIPYNP SSHESLDQVG EEKEAMNTRE
250 260 270 280 290 300
SGKASSSLGL QDFDLLRVIG RGSYAKVLLV RLKKTDRIYA MKVVKKELVN DDEDIDWVQT
310 320 330 340 350 360
EKHVFEQASN HPFLVGLHSC FQTESRLFFV IEYVNGGDLM FHMQRQRKLP EEHARFYSAE
370 380 390 400 410 420
ISLALNYLHE RGIIYRDLKL DNVLLDSEGH IKLTDYGMCK EGLRPGDTTS TFCGTPNYIA
430 440 450 460 470 480
PEILRGEDYG FSVDWWALGV LMFEMMAGRS PFDIVGSSDN PDQNTEDYLF QVILEKQIRI
490 500 510 520 530 540
PRSLSVKAAS VLKSFLNKDP KERLGCHPQT GFADIQGHPF FRNVDWDMME QKQVVPPFKP
550 560 570 580 590
NISGEFGLDN FDSQFTNEPV QLTPDDDDIV RKIDQSEFEG FEYINPLLMS AEECV