Q61768
Gene name |
Kif5b (Khcs, Kns1) |
Protein name |
Kinesin-1 heavy chain |
Names |
Conventional kinesin heavy chain , Ubiquitous kinesin heavy chain , UKHC |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:16573 |
EC number |
|
Protein Class |
CENTROMERE PROTEIN E (PTHR47968) |

Descriptions
Kinesin-1 heavy chain (Kif5b) is a member of the kinesin family of motor proteins and plays an essential role in the normal distribution of mitochondria and lysosomes. Kif5b is autoinhibited by the phosphorylation at Ser-175, which reduces the motor's stall force and modifies the ATPase activity, potentially affecting the microtubule-based transport, especially under loads. Both phosphorylation and S175D mutation decrease the stall force of kinesin, thereby stabilizing the autoinhibition of kinesin.
Autoinhibitory domains (AIDs)
Target domain |
8-325 (Kinesin motor domain) |
Relief mechanism |
PTM |
Assay |
Structural analysis, Deletion assay, Mutagenesis experiment |
Accessory elements
No accessory elements
Autoinhibited structure

Activated structure

3 structures for Q61768
Entry ID | Method | Resolution | Chain | Position | Source |
---|---|---|---|---|---|
6IGN | X-ray | 345 A | A | 444-566 | PDB |
6IGV | X-ray | 300 A | A | 446-563 | PDB |
AF-Q61768-F1 | Predicted | AlphaFoldDB |
36 variants for Q61768
Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
---|---|---|---|---|---|
rs13464374 | 8 | N>D | No | EVA | |
rs3389480852 | 239 | S>DG* | No | EVA | |
rs3389397489 | 240 | K>R | No | EVA | |
rs3408029296 | 335 | L>F | No | EVA | |
rs3407555574 | 338 | E>A | No | EVA | |
rs3408246669 | 338 | E>Q | No | EVA | |
rs3389489960 | 367 | R>L | No | EVA | |
rs260197391 | 392 | A>T | No | EVA | |
rs29879768 | 396 | I>T | No | EVA | |
rs3389478314 | 403 | P>L | No | EVA | |
rs3389397552 | 449 | L>M | No | EVA | |
rs3389490000 | 503 | D>E | No | EVA | |
rs3389454131 | 506 | S>F | No | EVA | |
rs3408201313 | 539 | K>M | No | EVA | |
rs3389454089 | 539 | K>N | No | EVA | |
rs3407555634 | 539 | K>Q | No | EVA | |
rs3389478313 | 542 | E>V | No | EVA | |
rs3389454149 | 555 | M>K | No | EVA | |
rs3411999166 | 562 | L>I | No | EVA | |
rs3389486031 | 621 | K>* | No | EVA | |
rs3389444756 | 645 | S>P | No | EVA | |
rs3389493534 | 697 | V>A | No | EVA | |
rs3389493492 | 718 | S>G | No | EVA | |
rs3389498054 | 736 | Q>P | No | EVA | |
rs3389434452 | 743 | E>V | No | EVA | |
rs3389486093 | 749 | V>* | No | EVA | |
rs3408100552 | 798 | N>T | No | EVA | |
rs3408276204 | 805 | Q>* | No | EVA | |
rs3389478312 | 812 | K>T | No | EVA | |
rs3389486973 | 860 | L>F | No | EVA | |
rs3389489986 | 872 | E>Q | No | EVA | |
rs3389487046 | 877 | L>S | No | EVA | |
rs3389444752 | 913 | R>G | No | EVA | |
rs3389454111 | 917 | S>P | No | EVA | |
rs3389434451 | 938 | G>D | No | EVA | |
rs3389465806 | 938 | G>R | No | EVA |
1 associated diseases with Q61768
[MIM: 277590]: Weaver syndrome (WVS)
A syndrome of accelerated growth and osseous maturation, unusual craniofacial appearance, hoarse and low-pitched cry, and hypertonia with camptodactyly. Distinguishing features of Weaver syndrome include broad forehead and face, ocular hypertelorism, prominent wide philtrum, micrognathia, deep horizontal chin groove, and deep-set nails. In addition, carpal bone development is advanced over the rest of the hand. . Note=The disease is caused by variants affecting the gene represented in this entry.
Without disease ID
- A syndrome of accelerated growth and osseous maturation, unusual craniofacial appearance, hoarse and low-pitched cry, and hypertonia with camptodactyly. Distinguishing features of Weaver syndrome include broad forehead and face, ocular hypertelorism, prominent wide philtrum, micrognathia, deep horizontal chin groove, and deep-set nails. In addition, carpal bone development is advanced over the rest of the hand. . Note=The disease is caused by variants affecting the gene represented in this entry.
9 regional properties for Q61768
Type | Name | Position | InterPro Accession |
---|---|---|---|
domain | SANT/Myb domain | 159 - 250 | IPR001005-1 |
domain | SANT/Myb domain | 428 - 476 | IPR001005-2 |
domain | SET domain | 612 - 733 | IPR001214 |
domain | CXC domain | 503 - 605 | IPR026489 |
domain | Tesmin/TSO1-like CXC domain | 555 - 592 | IPR033467 |
domain | Polycomb repressive complex 2 subunit EZH1/EZH2, tri-helical domain | 158 - 249 | IPR041343 |
domain | Pre-SET CXC domain | 559 - 590 | IPR041355 |
domain | EZH2, SET domain | 609 - 728 | IPR044439 |
domain | EZH1/2, MCSS domain | 257 - 309 | IPR048358 |
Functions
Description | ||
---|---|---|
EC Number | ||
Subcellular Localization |
|
|
PANTHER Family | PTHR47968 | CENTROMERE PROTEIN E |
PANTHER Subfamily | PTHR47968:SF73 | KINESIN HEAVY CHAIN ISOFORM X1 |
PANTHER Protein Class | ||
PANTHER Pathway Category | No pathway information available |
18 GO annotations of cellular component
Name | Definition |
---|---|
axon cytoplasm | Any cytoplasm that is part of a axon. |
axonal growth cone | The migrating motile tip of a growing nerve cell axon. |
centriolar satellite | A small (70-100 nm) cytoplasmic granule that contains a number of centrosomal proteins; centriolar satellites traffic toward microtubule minus ends and are enriched near the centrosome. |
ciliary rootlet | A cytoskeleton-like structure, originating from the basal body at the proximal end of a cilium, and extending proximally toward the cell nucleus. Rootlets are typically 80-100 nm in diameter and contain cross striae distributed at regular intervals of approximately 55-70 nm. |
cytolytic granule membrane | The lipid bilayer surrounding the cytolytic granule. |
cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
dendrite cytoplasm | All of the contents of a dendrite, excluding the surrounding plasma membrane. |
endocytic vesicle | A membrane-bounded intracellular vesicle formed by invagination of the plasma membrane around an extracellular substance. Endocytic vesicles fuse with early endosomes to deliver the cargo for further sorting. |
kinesin complex | Any complex that includes a dimer of molecules from the kinesin superfamily, a group of related proteins that contain an extended region of predicted alpha-helical coiled coil in the main chain that likely produces dimerization. The native complexes of several kinesin family members have also been shown to contain additional peptides, often designated light chains as all of the noncatalytic subunits that are currently known are smaller than the chain that contains the motor unit. Kinesin complexes generally possess a force-generating enzymatic activity, or motor, which converts the free energy of the gamma phosphate bond of ATP into mechanical work. |
membrane-bounded organelle | Organized structure of distinctive morphology and function, bounded by a single or double lipid bilayer membrane. Includes the nucleus, mitochondria, plastids, vacuoles, and vesicles. Excludes the plasma membrane. |
microtubule | Any of the long, generally straight, hollow tubes of internal diameter 12-15 nm and external diameter 24 nm found in a wide variety of eukaryotic cells; each consists (usually) of 13 protofilaments of polymeric tubulin, staggered in such a manner that the tubulin monomers are arranged in a helical pattern on the microtubular surface, and with the alpha/beta axes of the tubulin subunits parallel to the long axis of the tubule; exist in equilibrium with pool of tubulin monomers and can be rapidly assembled or disassembled in response to physiological stimuli; concerned with force generation, e.g. in the spindle. |
microtubule cytoskeleton | The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins. |
mitochondrion | A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration. |
neuron projection | A prolongation or process extending from a nerve cell, e.g. an axon or dendrite. |
perinuclear region of cytoplasm | Cytoplasm situated near, or occurring around, the nucleus. |
phagocytic vesicle | A membrane-bounded intracellular vesicle that arises from the ingestion of particulate material by phagocytosis. |
vesicle | Any small, fluid-filled, spherical organelle enclosed by membrane. |
9 GO annotations of molecular function
Name | Definition |
---|---|
ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
ATP hydrolysis activity | Catalysis of the reaction |
identical protein binding | Binding to an identical protein or proteins. |
JUN kinase binding | Binding to JUN kinase, an enzyme that catalyzes the phosphorylation and activation of members of the JUN family. |
microtubule binding | Binding to a microtubule, a filament composed of tubulin monomers. |
microtubule lateral binding | Binding to the side of a microtubule. |
microtubule motor activity | A motor activity that generates movement along a microtubule, driven by ATP hydrolysis. |
plus-end-directed microtubule motor activity | A motor activity that generates movement along a microtubule toward the plus end, driven by ATP hydrolysis. |
protein-containing complex binding | Binding to a macromolecular complex. |
24 GO annotations of biological process
Name | Definition |
---|---|
anterograde axonal protein transport | The directed movement of proteins along microtubules from the cell body toward the cell periphery in nerve cell axons. |
anterograde dendritic transport of neurotransmitter receptor complex | The directed movement of a neurotransmitter receptor complex along microtubules in nerve cell dendrites towards the postsynapse. |
axon guidance | The chemotaxis process that directs the migration of an axon growth cone to a specific target site in response to a combination of attractive and repulsive cues. |
cellular response to type II interferon | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interferon-gamma stimulus. Interferon gamma is the only member of the type II interferon found so far. |
centrosome localization | Any process in which a centrosome is transported to, and/or maintained in, a specific location within the cell. |
cytoplasm organization | A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of the cytoplasm. The cytoplasm is all of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
lysosome localization | Any process in which a lysosome is transported to, and/or maintained in, a specific location. |
microtubule-based process | Any cellular process that depends upon or alters the microtubule cytoskeleton, that part of the cytoskeleton comprising microtubules and their associated proteins. |
mitochondrial transport | Transport of substances into, out of or within a mitochondrion. |
mitochondrion transport along microtubule | The directed movement of a mitochondrion along a microtubule, mediated by motor proteins. |
mitocytosis | A migrasome-mediated selective removal of damaged mitochondria process that maintains mitochondrion homeostasis in migrating cells. |
natural killer cell mediated cytotoxicity | The directed killing of a target cell by a natural killer cell through the release of granules containing cytotoxic mediators or through the engagement of death receptors. |
plus-end-directed vesicle transport along microtubule | The directed movement of a vesicle towards the plus end of a microtubule, mediated by motor proteins. This process begins with the attachment of a vesicle to a microtubule, and ends when the vesicle reaches its final destination. |
positive regulation of insulin secretion involved in cellular response to glucose stimulus | Any process that increases the frequency, rate or extent of the regulated release of insulin that contributes to the response of a cell to glucose. |
positive regulation of intracellular protein transport | Any process that activates or increases the frequency, rate or extent of the directed movement of proteins within cells. |
positive regulation of potassium ion transport | Any process that activates or increases the frequency, rate or extent of the directed movement of potassium ions (K+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
positive regulation of protein localization to plasma membrane | Any process that activates or increases the frequency, rate or extent of protein localization to plasma membrane. |
positive regulation of synaptic transmission, GABAergic | Any process that activates, maintains or increases the frequency, rate or extent of GABAergic synaptic transmission, the process of communication from a neuron to another neuron across a synapse using the neurotransmitter gamma-aminobutyric acid (GABA). |
positive regulation of vesicle fusion | Any process that activates or increases the frequency, rate or extent of vesicle fusion. |
regulation of membrane potential | Any process that modulates the establishment or extent of a membrane potential, the electric potential existing across any membrane arising from charges in the membrane itself and from the charges present in the media on either side of the membrane. |
retrograde neuronal dense core vesicle transport | The directed movement of neuronal dense core vesicles along axonal microtubules towards the cell body. |
stress granule disassembly | The disaggregation of a stress granule into its constituent protein and RNA parts. |
synaptic vesicle transport | The directed movement of synaptic vesicles. |
vesicle transport along microtubule | The directed movement of a vesicle along a microtubule, mediated by motor proteins. This process begins with the attachment of a vesicle to a microtubule, and ends when the vesicle reaches its final destination. |
16 homologous proteins in AiPD
UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
---|---|---|---|---|
P17210 | Khc | Kinesin heavy chain | Drosophila melanogaster (Fruit fly) | EV |
O60282 | KIF5C | Kinesin heavy chain isoform 5C | Homo sapiens (Human) | EV |
P33176 | KIF5B | Kinesin-1 heavy chain | Homo sapiens (Human) | EV |
Q12840 | KIF5A | Kinesin heavy chain isoform 5A | Homo sapiens (Human) | EV |
P28738 | Kif5c | Kinesin heavy chain isoform 5C | Mus musculus (Mouse) | SS |
P33175 | Kif5a | Kinesin heavy chain isoform 5A | Mus musculus (Mouse) | SS |
Q99PW8 | Kif17 | Kinesin-like protein KIF17 | Mus musculus (Mouse) | SS |
Q7M6Z4 | Kif27 | Kinesin-like protein KIF27 | Mus musculus (Mouse) | SS |
Q9QXL2 | Kif21a | Kinesin-like protein KIF21A | Mus musculus (Mouse) | EV SS |
Q9QXL1 | Kif21b | Kinesin-like protein KIF21B | Mus musculus (Mouse) | SS |
Q9EQW7 | Kif13a | Kinesin-like protein KIF13A | Mus musculus (Mouse) | SS |
B7ZNG0 | Kif7 | Kinesin-like protein KIF7 | Mus musculus (Mouse) | SS |
Q6QLM7 | Kif5a | Kinesin heavy chain isoform 5A | Rattus norvegicus (Rat) | SS |
Q2PQA9 | Kif5b | Kinesin-1 heavy chain | Rattus norvegicus (Rat) | SS |
P34540 | unc-116 | Kinesin heavy chain | Caenorhabditis elegans | SS |
Q9SV36 | KINUC | Kinesin-like protein KIN-UC | Arabidopsis thaliana (Mouse-ear cress) | PR |
10 | 20 | 30 | 40 | 50 | 60 |
MADPAECNIK | VMCRFRPLNE | SEVNRGDKYV | AKFQGEDTVM | IASKPYAFDR | VFQSSTSQEQ |
70 | 80 | 90 | 100 | 110 | 120 |
VYNDCAKKIV | KDVLEGYNGT | IFAYGQTSSG | KTHTMEGKLH | DPEGMGIIPR | IVQDIFNYIY |
130 | 140 | 150 | 160 | 170 | 180 |
SMDENLEFHI | KVSYFEIYLD | KIRDLLDVSK | TNLSVHEDKN | RVPYVKGCTE | RFVCSPDEVM |
190 | 200 | 210 | 220 | 230 | 240 |
DTIDEGKSNR | HVAVTNMNEH | SSRSHSIFLI | NVKQENTQTE | QKLSGKLYLV | DLAGSEKVSK |
250 | 260 | 270 | 280 | 290 | 300 |
TGAEGAVLDE | AKNINKSLSA | LGNVISALAE | GSTYVPYRDS | KMTRILQDSL | GGNCRTTIVI |
310 | 320 | 330 | 340 | 350 | 360 |
CCSPSSYNES | ETKSTLLFGQ | RAKTIKNTVC | VNVELTAEQW | KKKYEKEKEK | NKTLRNTIQW |
370 | 380 | 390 | 400 | 410 | 420 |
LENELNRWRN | GETVPIDEQF | DKEKANLEAF | TADKDIAITS | DKPAAAVGMA | GSFTDAERRK |
430 | 440 | 450 | 460 | 470 | 480 |
CEEELAKLYK | QLDDKDEEIN | QQSQLVEKLK | TQMLDQEELL | ASTRRDQDNM | QAELNRLQAE |
490 | 500 | 510 | 520 | 530 | 540 |
NDASKEEVKE | VLQALEELAV | NYDQKSQEVE | DKTKEYELLS | DELNQKSATL | ASIDAELQKL |
550 | 560 | 570 | 580 | 590 | 600 |
KEMTNHQKKR | AAEMMASLLK | DLAEIGIAVG | NNDVKQPEGT | GMIDEEFTVA | RLYISKMKSE |
610 | 620 | 630 | 640 | 650 | 660 |
VKTMVKRCKQ | LESTQTESNK | KMEENEKELA | ACQLRISQHE | AKIKSLTEYL | QNVEQKKRQL |
670 | 680 | 690 | 700 | 710 | 720 |
EESVDSLGEE | LVQLRAQEKV | HEMEKEHLNK | VQTANEVKQA | VEQQIQSHRE | THQKQISSLR |
730 | 740 | 750 | 760 | 770 | 780 |
DEVEAKEKLI | TDLQDQNQKM | VLEQERLRVE | HERLKATDQE | KSRKLHELTV | MQDRREQARQ |
790 | 800 | 810 | 820 | 830 | 840 |
DLKGLEETVA | KELQTLHNLR | KLFVQDLATR | VKKSAEVDSD | DTGGSAAQKQ | KISFLENNLE |
850 | 860 | 870 | 880 | 890 | 900 |
QLTKVHKQLV | RDNADLRCEL | PKLEKRLRAT | AERVKALESA | LKEAKENASR | DRKRYQQEVD |
910 | 920 | 930 | 940 | 950 | 960 |
RIKEAVRSKN | MARRGHSAQI | AKPIRPGQHP | AASPTHPGTV | RGGGSFVQNN | QPVGLRGGGG |
KQS |