Q61161

SS
Gene name
Map4k2 (Rab8ip)
Protein name
Mitogen-activated protein kinase kinase kinase kinase 2
Names
Germinal center kinase, GCK, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, MEKKK 2, Rab8-interacting protein
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:26412
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

MAP4K2 is a serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway.?In human MAP4K2 (Q12851), its autoinhibitory domain is within the Leu-rich domain. It inhibits MAPK/ERK kinase binding (reversible, stimulus-induced membrane translocation, which is required for activation) possibly via structural motifs.

Autoinhibitory domains (AIDs)

467-671 (Autoinhibitory region) SS

Target domain

16-273 (Protein kinase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

153-176 (Activation loop from InterPro)

Target domain

16-273 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q61161

Entry ID Method Resolution Chain Position Source
AF-Q61161-F1 Predicted AlphaFoldDB

4 variants for Q61161

Variant ID(s) Position Change Description Diseaes Association Provenance
rs243692821 66 R>P No Ensembl
rs13472963 292 P>H No Ensembl
rs37226783 418 G>D No Ensembl
rs246302175 651 M>L No Ensembl

No associated diseases with Q61161

5 regional properties for Q61161

Type Name Position InterPro Accession
domain Protein kinase domain 406 - 663 IPR000719
domain Doublecortin domain 52 - 143 IPR003533-1
domain Doublecortin domain 181 - 269 IPR003533-2
active_site Serine/threonine-protein kinase, active site 523 - 535 IPR008271
binding_site Protein kinase, ATP binding site 412 - 439 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Basolateral cell membrane ; Peripheral membrane protein
  • Golgi apparatus membrane ; Peripheral membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
basolateral plasma membrane The region of the plasma membrane that includes the basal end and sides of the cell. Often used in reference to animal polarized epithelial membranes, where the basal membrane is the part attached to the extracellular matrix, or in plant cells, where the basal membrane is defined with respect to the zygotic axis.
Golgi membrane The lipid bilayer surrounding any of the compartments of the Golgi apparatus.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
MAP kinase kinase kinase kinase activity Catalysis of the phosphorylation of serine and threonine residues in a mitogen-activated protein kinase kinase kinase (MAPKKK), resulting in activation of MAPKKK. MAPKKK signaling pathways relay, amplify and integrate signals from the plasma membrane to the nucleus in response to a diverse range of extracellular stimuli.
mitogen-activated protein kinase kinase kinase binding Binding to a mitogen-activated protein kinase kinase kinase, a protein that can phosphorylate a MAP kinase kinase.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

6 GO annotations of biological process

Name Definition
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
positive regulation of JNK cascade Any process that activates or increases the frequency, rate or extent of signal transduction mediated by the JNK cascade.
positive regulation of JUN kinase activity Any process that activates or increases the frequency, rate or extent of JUN kinase activity.
protein phosphorylation The process of introducing a phosphate group on to a protein.
vesicle targeting The process in which vesicles are directed to specific destination membranes. Targeting involves coordinated interactions among cytoskeletal elements (microtubules or actin filaments), motor proteins, molecules at the vesicle membrane and target membrane surfaces, and vesicle cargo.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q8IVH8 MAP4K3 Mitogen-activated protein kinase kinase kinase kinase 3 Homo sapiens (Human) SS
Q92918 MAP4K1 Mitogen-activated protein kinase kinase kinase kinase 1 Homo sapiens (Human) EV
Q9Y4K4 MAP4K5 Mitogen-activated protein kinase kinase kinase kinase 5 Homo sapiens (Human) PR
Q12851 MAP4K2 Mitogen-activated protein kinase kinase kinase kinase 2 Homo sapiens (Human) EV
P70218 Map4k1 Mitogen-activated protein kinase kinase kinase kinase 1 Mus musculus (Mouse) PR
Q99JP0 Map4k3 Mitogen-activated protein kinase kinase kinase kinase 3 Mus musculus (Mouse) SS
O54988 Slk STE20-like serine/threonine-protein kinase Mus musculus (Mouse) PR
O55098 Stk10 Serine/threonine-protein kinase 10 Mus musculus (Mouse) PR
Q6P9R2 Oxsr1 Serine/threonine-protein kinase OSR1 Mus musculus (Mouse) PR
Q9Z1W9 Stk39 STE20/SPS1-related proline-alanine-rich protein kinase Mus musculus (Mouse) PR
Q99JT2 Stk26 Serine/threonine-protein kinase 26 Mus musculus (Mouse) PR
Q99KH8 Stk24 Serine/threonine-protein kinase 24 Mus musculus (Mouse) PR
Q9Z2W1 Stk25 Serine/threonine-protein kinase 25 Mus musculus (Mouse) PR
Q8BPM2 Map4k5 Mitogen-activated protein kinase kinase kinase kinase 5 Mus musculus (Mouse) PR
Q924I2 Map4k3 Mitogen-activated protein kinase kinase kinase kinase 3 Rattus norvegicus (Rat) PR
10 20 30 40 50 60
MALLRDVSLQ DPRDRFELLQ RVGAGTYGDV YKARDTVTSE LAAVKIVKLD PGDDISSLQQ
70 80 90 100 110 120
EITILRECRH PNVVAYIGSY LRNDRLWICM EFCGGGSLQE IYHATGPLEE RQIAYVCREA
130 140 150 160 170 180
LKGLHHLHSQ GKIHRDIKGA NLLLTLQGDV KLADFGVSGE LTASVAKRRS FIGTPYWMAP
190 200 210 220 230 240
EVAAVERKGG YNELCDVWAL GITAIELGEL QPPLFHLHPM RALMLMSKSS FQPPKLRDKT
250 260 270 280 290 300
RWTQNFHHFL KLALTKNPKK RPTAERLLQH PFTTQHLPPA LLTQLLDKAS DPHLGTLSPE
310 320 330 340 350 360
DSELETHDMF PDTIHSRSHH GPAERTPSEI QFHQVKFGAP RRKETDPLNE PWEEEWTLLG
370 380 390 400 410 420
KEELSGSLLQ SVQEALEERS LTIRPALELQ ELDSPDDAIG TIKRAPFLGL PHTESTSGDN
430 440 450 460 470 480
AQSCSPGTLS APPAGPGSPA LLPTAWATLK QQEDRERSSC HGLPPTPKVH MGACFSKVFN
490 500 510 520 530 540
GCPLQIHAAV TWVHPVTRDQ FLVVGAEEGI YTLNLHELHE DTMEKLISQR CSWLYCVNNV
550 560 570 580 590 600
LLSLSGKSTH IWAHDLPGLF EQRRLQHQAP LSIPTNRITQ RIIPRRFALS TKIPDTKGCL
610 620 630 640 650 660
QCRVVRNPYT GSTFLLAALP ASLLLLQWYE PLQKFLLLKN FSSPLPSPAG MLEPLVLDGK
670 680 690 700 710 720
ELPQVCVGAE GPEGPGCRVL FHVLPLEAGL TPDILIPPEG IPGSAQQVIQ VDRDTVLVSF
730 740 750 760 770 780
ERCVRIVNLQ GEPTAALAPE LTFDFTIETV VCLQDSVLAF WSHGMQGRSL DTNEVTQEIT
790 800 810 820
DETRIFRVLG AHRDIILESI PTDNPGAHSN LYILTGHQSS Y