Q5ZM36

PR
Gene name
EIF4A3 (DDX48, RCJMB04_3e17)
Protein name
Eukaryotic initiation factor 4A-III
Names
eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic translation initiation factor 4A isoform 3
Species
Gallus gallus (Chicken)
KEGG Pathway
gga:416704
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

1-68 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q5ZM36

Entry ID Method Resolution Chain Position Source
AF-Q5ZM36-F1 Predicted AlphaFoldDB

7 variants for Q5ZM36

Variant ID(s) Position Change Description Diseaes Association Provenance
rs739677239 221 T>P No Ensembl
rs312473107 259 V>G No Ensembl
rs741081594 260 E>G No Ensembl
rs730914877 262 E>G No Ensembl
rs739086962 279 T>P No Ensembl
rs317481850 285 C>* No Ensembl
rs734306047 292 D>E No Ensembl

No associated diseases with Q5ZM36

5 regional properties for Q5ZM36

Type Name Position InterPro Accession
conserved_site ATP-dependent RNA helicase DEAD-box, conserved site 186 - 194 IPR000629
domain Helicase, C-terminal 251 - 412 IPR001650
domain DEAD/DEAH box helicase domain 64 - 226 IPR011545
domain Helicase superfamily 1/2, ATP-binding domain 58 - 255 IPR014001
domain RNA helicase, DEAD-box type, Q motif 39 - 67 IPR014014

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
  • Nucleus
  • Nucleus speckle
  • Cytoplasm
  • Nucleocytoplasmic shuttling protein
  • Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

6 GO annotations of cellular component

Name Definition
catalytic step 2 spliceosome A spliceosomal complex that contains three snRNPs, including U5, bound to a splicing intermediate in which the first catalytic cleavage of the 5' splice site has occurred. The precise subunit composition differs significantly from that of the catalytic step 1, or activated, spliceosome, and includes many proteins in addition to those found in the associated snRNPs.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nuclear speck A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy.
nucleolus A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
U2-type catalytic step 1 spliceosome A spliceosomal complex that is formed by the displacement of the U1 and U4 snRNPs from the precatalytic spliceosome; the U2, U5 and U6 snRNPs remain associated with the mRNA. This complex, sometimes called the activated spliceosome, is the catalytically active form of the spliceosome, and includes many proteins in addition to those found in the U2, and U5 and U6 snRNPs.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient.
mRNA binding Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.
RNA binding Binding to an RNA molecule or a portion thereof.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

5 GO annotations of biological process

Name Definition
mRNA splicing, via spliceosome The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.
mRNA transport The directed movement of mRNA, messenger ribonucleic acid, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay The nonsense-mediated decay pathway for nuclear-transcribed mRNAs degrades mRNAs in which an amino-acid codon has changed to a nonsense codon; this prevents the translation of such mRNAs into truncated, and potentially harmful, proteins.
regulation of alternative mRNA splicing, via spliceosome Any process that modulates the frequency, rate or extent of alternative splicing of nuclear mRNAs.
regulation of translation Any process that modulates the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA.

14 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q12099 FAL1 ATP-dependent RNA helicase FAL1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
Q2NL22 EIF4A3 Eukaryotic initiation factor 4A-III Bos taurus (Bovine) PR
Q8JFP1 EIF4A2 Eukaryotic initiation factor 4A-II Gallus gallus (Chicken) PR
Q5ZHZ0 DDX39B Spliceosome RNA helicase DDX39B Gallus gallus (Chicken) PR
Q9VHS8 CG7483 Eukaryotic initiation factor 4A-III Drosophila melanogaster (Fruit fly) PR
P38919 EIF4A3 Eukaryotic initiation factor 4A-III Homo sapiens (Human) PR
Q91VC3 Eif4a3 Eukaryotic initiation factor 4A-III Mus musculus (Mouse) PR
A6M931 EIF4A3 Eukaryotic initiation factor 4A-III Sus scrofa (Pig) PR
Q3B8Q2 Eif4a3 Eukaryotic initiation factor 4A-III Rattus norvegicus (Rat) PR
Q10I26 EIF4A3B Eukaryotic initiation factor 4A-III homolog B Oryza sativa subsp japonica (Rice) PR
Q5VNM3 EIF4A3A Eukaryotic initiation factor 4A-III homolog A Oryza sativa subsp japonica (Rice) PR
Q94A52 EIF4A3 Eukaryotic initiation factor 4A-III homolog Arabidopsis thaliana (Mouse-ear cress) PR
B7ZTW1 eif4a3 Eukaryotic initiation factor 4A-III Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
Q7ZVA6 eif4a3 Eukaryotic initiation factor 4A-III Danio rerio (Zebrafish) (Brachydanio rerio) PR
10 20 30 40 50 60
MSGSAGSGGT TGSARKRIMK EEDMTKVEFE TSEEVDVTPT FDTMGLREDL LRGIYAYGFE
70 80 90 100 110 120
KPSAIQQRAI KQIIKGRDVI AQSQSGTGKT ATFSISVLQC LDIQVRETQA LILAPTRELA
130 140 150 160 170 180
VQIQKGLLAL GDYMNVQCHA CIGGTNVGED IRKLDYGQHV VAGTPGRVFD MIRRRSLRTR
190 200 210 220 230 240
AIKMLVLDEA DEMLNKGFKE QIYDVYRYLP PATQVVLISA TLPHEILEMT NKFMTDPIRI
250 260 270 280 290 300
LVKRDELTLE GIKQFFVAVE REEWKFDTLC DLYDTLTITQ AVIFCNTKRK VDWLTEKMRE
310 320 330 340 350 360
ANFTVSSMHG DMPQKERESI MKEFRSGASR VLISTDVWAR GLDVPQVSLI INYDLPNNRE
370 380 390 400 410
LYIHRIGRSG RYGRKGVAIN FVKNDDIRIL RDIEQYYSTQ IDEMPMNVAD LI