Q5ZM14
SS
Gene name |
NHERF1 (NHERF, SLC9A3R1, RCJMB04_3g21) |
Protein name |
Na |
Names |
+/H, + exchange regulatory cofactor NHE-RF1 , NHERF-1 , Ezrin-radixin-moesin-binding phosphoprotein 50 , EBP50 , Regulatory cofactor of Na, +/H, + exchanger , Sodium-hydrogen exchanger regulatory factor 1 , Solute carrier family 9 isoform A3 regulatory factor 1 |
Species |
Gallus gallus (Chicken) |
KEGG Pathway |
gga:422108 |
EC number |
|
Protein Class |
|
Descriptions
The mammalian Na+/H+ exchange regulatory factor 1 (NHERF1) is a multidomain scaffolding protein essential for regulating the intracellular trafficking and macromolecular assembly of transmembrane ion channels and receptors. NHERF1 is held in an autoinhibited state through intramolecular interactions between PDZ2 and the CT domain that also includes a C-terminal PDZ-binding motif (-SNL). A central feature of NHERF1 is its binding to ezrin and to other ezrin-radixin-moesin (ERM) proteins, forming a communication bridge between plasma membrane proteins and the actin cytoskeleton ezrin modulates the PDZ domains of NHERF1 to assemble multiprotein complexes in a cooperative fashion. Upon binding to ezrin, the intramolecular domain-domain interaction is released.
Autoinhibitory domains (AIDs)
Target domain |
13-93 (PDZ1 domain);135-215 (PDZ2 domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q5ZM14
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q5ZM14-F1 | Predicted | AlphaFoldDB |
No variants for Q5ZM14
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q5ZM14 | |||||
No associated diseases with Q5ZM14
6 regional properties for Q5ZM14
| Type | Name | Position | InterPro Accession |
|---|---|---|---|
| domain | SNF2, N-terminal | 49 - 327 | IPR000330 |
| domain | Helicase, C-terminal domain-like | 348 - 513 | IPR001650 |
| conserved_site | DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site | 169 - 178 | IPR002464 |
| domain | Macro domain | 704 - 897 | IPR002589 |
| domain | Helicase superfamily 1/2, ATP-binding domain | 42 - 230 | IPR014001 |
| domain | SNF2/RAD5-like, C-terminal helicase domain | 346 - 470 | IPR049730 |
8 GO annotations of cellular component
| Name | Definition |
|---|---|
| apical plasma membrane | The region of the plasma membrane located at the apical end of the cell. |
| cell periphery | The broad region around and including the plasma membrane of a cell, encompassing the cell cortex (inside the cell), the plasma membrane, and any external encapsulating structures. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| endomembrane system | A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles. |
| filopodium | Thin, stiff, actin-based protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal or dendritic growth cone, or a dendritic shaft. |
| microvillus | Thin cylindrical membrane-covered projections on the surface of an animal cell containing a core bundle of actin filaments. Present in especially large numbers on the absorptive surface of intestinal cells. |
| microvillus membrane | The portion of the plasma membrane surrounding a microvillus. |
| ruffle | Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| chloride channel regulator activity | Binds to and modulates the activity of a chloride channel. |
| protein-membrane adaptor activity | The binding activity of a molecule that brings together a protein or a protein complex with a membrane, or bringing together two membranes, either via membrane lipid binding or by interacting with a membrane protein, to establish or maintain the localization of the protein, protein complex or organelle. |
| signaling receptor binding | Binding to one or more specific sites on a receptor molecule, a macromolecule that undergoes combination with a hormone, neurotransmitter, drug or intracellular messenger to initiate a change in cell function. |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| bile acid secretion | The regulated release of bile acid, composed of any of a group of steroid carboxylic acids occurring in bile, by a cell or a tissue. |
| glutathione transport | The directed movement of glutathione, the tripeptide glutamylcysteinylglycine, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| negative regulation of cell motility | Any process that stops, prevents, or reduces the frequency, rate or extent of cell motility. |
| negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction | Any process that stops, prevents, or reduces the frequency, rate or extent of protein kinase B signaling, a series of reactions mediated by the intracellular serine/threonine kinase protein kinase B. |
| negative regulation of platelet-derived growth factor receptor signaling pathway | Any process that stops, prevents, or reduces the frequency, rate or extent of the platelet-derived growth factor receptor signaling pathway. |
| protein localization to plasma membrane | A process in which a protein is transported to, or maintained in, a specific location in the plasma membrane. |
| regulation of protein kinase activity | Any process that modulates the frequency, rate or extent of protein kinase activity. |
| renal absorption | A renal system process in which water, ions, glucose and proteins are taken up from the collecting ducts, glomerulus and proximal and distal loops of the nephron. In non-mammalian species, absorption may occur in related structures (e.g. protein absorption is observed in nephrocytes in Drosophila, see PMID:23264686). |
| Wnt signaling pathway | The series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state. |
11 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q3SZK8 | NHERF1 | Na | Bos taurus (Bovine) | SS |
| Q24008 | inaD | Inactivation-no-after-potential D protein | Drosophila melanogaster (Fruit fly) | PR |
| Q9Y6N9 | USH1C | Harmonin | Homo sapiens (Human) | PR |
| Q86UT5 | PDZD3 | Na(+)/H(+) exchange regulatory cofactor NHE-RF4 | Homo sapiens (Human) | PR |
| O14745 | SLC9A3R1 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | Homo sapiens (Human) | EV |
| Q99MJ6 | Pdzd3 | Na(+)/H(+) exchange regulatory cofactor NHE-RF4 | Mus musculus (Mouse) | PR |
| Q9JIL4 | Pdzk1 | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 | Mus musculus (Mouse) | PR |
| Q0QWG9 | Grid2ip | Delphilin | Mus musculus (Mouse) | PR |
| P70441 | Slc9a3r1 | Na(+)/H(+) exchange regulatory cofactor NHE-RF1 | Mus musculus (Mouse) | PR |
| Q9JJ40 | Pdzk1 | Na(+)/H(+) exchange regulatory cofactor NHE-RF3 | Rattus norvegicus (Rat) | PR |
| Q9JJ19 | Nherf1 | Na | Rattus norvegicus (Rat) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSSAPPGPAA | PRLCCMEKGP | DGYGFHLHGE | KGKPGQYIRL | VEAGSPAERS | GLRAGDRLLE |
| 70 | 80 | 90 | 100 | 110 | 120 |
| VDGTNVERES | HQQVVERIRA | AAGAVRLLVV | QPQPEEQPPK | THSDPDGEAQ | REPPAAETPA |
| 130 | 140 | 150 | 160 | 170 | 180 |
| AERSGPEERE | LRPRLCRIKK | GPNGYGFNLH | SEKSRPGQYV | RAVDPDSPAE | AAGLRAQDRI |
| 190 | 200 | 210 | 220 | 230 | 240 |
| VEVNGTSVEG | KQHADVVAAI | KAGGDETKLL | VVGVLADEFF | KKCRVVPSEA | HLAGPLPEPM |
| 250 | 260 | 270 | 280 | 290 | 300 |
| ANGDVEKENG | GEPRLNSVSE | RPPSPALATS | PEGSETHSEP | DTQEGDKRSS | APSSLLDLDI |
| 310 | 320 | 330 | |||
| PLAVAKERAH | QKRTSKRAPQ | MDWSKKNELF | SNL |