Q5XIN1

SS
Gene name
Mdm4 (Mdmx)
Protein name
Protein Mdm4
Names
Double minute 4 protein , Mdm2-like p53-binding protein , Protein Mdmx , p53-binding protein Mdm4
Species
Rattus norvegicus (Rat)
KEGG Pathway
rno:304798
EC number
Protein Class

Descriptions

MDM4 is a homologous protein that binds to p53 and regulates its activity. Residues 190-210 in that intrinsically disordered region constitute an inhibitory module. It competes with the p53TAD for binding to the N-terminal SWIB-like domain (NTD) of MDMX, thus lowering the affinity of MDMX for p53. Efficient binding of MDMX to p53 requires a mechanism to relieve the allosteric self-inhibition.

Autoinhibitory domains (AIDs)

191-211 (Tryptophan-rich segment) SS

Target domain

26-109 (N-terminal SWIB-like domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q5XIN1

Entry ID Method Resolution Chain Position Source
AF-Q5XIN1-F1 Predicted AlphaFoldDB

No variants for Q5XIN1

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q5XIN1

No associated diseases with Q5XIN1

3 regional properties for Q5XIN1

Type Name Position InterPro Accession
domain Pyruvate kinase, barrel 19 - 345 IPR015793
domain Pyruvate kinase, C-terminal 380 - 497 IPR015795
active_site Pyruvate kinase, active site 235 - 247 IPR018209

Functions

Description
EC Number
Subcellular Localization
  • Nucleus
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
transcription repressor complex A protein complex that possesses activity that prevents or downregulates transcription.

3 GO annotations of molecular function

Name Definition
enzyme binding Binding to an enzyme, a protein with catalytic activity.
metal ion binding Binding to a metal ion.
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.

17 GO annotations of biological process

Name Definition
atrial septum development The progression of the atrial septum over time, from its initial formation to the mature structure.
atrioventricular valve morphogenesis The process in which the structure of the atrioventricular valve is generated and organized.
cellular response to hypoxia Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
DNA damage response, signal transduction by p53 class mediator A cascade of processes induced by the cell cycle regulator phosphoprotein p53, or an equivalent protein, in response to the detection of DNA damage.
endocardial cushion morphogenesis The process in which the anatomical structure of the endocardial cushion is generated and organized. The endocardial cushion is a specialized region of mesenchymal cells that will give rise to the heart septa and valves.
heart valve development The progression of a heart valve over time, from its formation to the mature structure. A heart valve is a structure that restricts the flow of blood to different regions of the heart and forms from an endocardial cushion.
negative regulation of DNA-templated transcription Any process that stops, prevents, or reduces the frequency, rate or extent of cellular DNA-templated transcription.
negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator Any process that stops, prevents or reduces the frequency, rate or extent of intrinsic apoptotic signaling pathway by p53 class mediator.
negative regulation of protein catabolic process Any process that stops, prevents or reduces the frequency, rate or extent of protein catabolic process.
negative regulation of transcription by RNA polymerase II Any process that stops, prevents, or reduces the frequency, rate or extent of transcription mediated by RNA polymerase II.
positive regulation of cell population proliferation Any process that activates or increases the rate or extent of cell proliferation.
protein stabilization Any process involved in maintaining the structure and integrity of a protein and preventing it from degradation or aggregation.
protein ubiquitination The process in which one or more ubiquitin groups are added to a protein.
protein-containing complex assembly The aggregation, arrangement and bonding together of a set of macromolecules to form a protein-containing complex.
regulation of cell cycle Any process that modulates the rate or extent of progression through the cell cycle.
regulation of heart rate Any process that modulates the frequency or rate of heart contraction.
ventricular septum development The progression of the ventricular septum over time from its formation to the mature structure.

5 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2HJ21 MDM4 Protein Mdm4 Bos taurus (Bovine) SS
E1C4B0 MDM4 Double minute 4 protein Gallus gallus (Chicken) SS
O15151 MDM4 Protein Mdm4 Homo sapiens (Human) EV
O35618 Mdm4 Protein Mdm4 Mus musculus (Mouse) SS
Q7ZUW7 mdm4 Protein Mdm4 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MTSHSTSAQC SASDSACRIS SEQINQQVRP KLQLLKILQA AGAQGEVFTM KEVMHYLGQY
70 80 90 100 110 120
IMVKQLYDQQ EQHLVYCGGD LLGDLLGCQS FSVKDPSPLY DMLRKNLVTS ASVNTDAAQT
130 140 150 160 170 180
LALAQDHTMD IPSQDRLKHG ATECSNPRKR TEEDDTHTLP TSRRKCRDSR ADEDLIEHLS
190 200 210 220 230 240
QDETSRLDLD FEEWDVAGLP WWFLGNLRNN YIPRSNGSTD LQTNQDIGTA IVSDTTDDLW
250 260 270 280 290 300
FLNETVSEQL GVGVKVEAAN CEQPSEVGRT RDKKMVEGGK DDDLEDSKSL SDDTDVEVTS
310 320 330 340 350 360
EDEWQCTECK KFNSPSKRYC FRCWALRKDW YSDCSKLTHS LSTSNITAIP EKKDNEGIDV
370 380 390 400 410 420
PDCRRTISAP VVRPKDGYLK EEKPRFDPCN SVEFLDLAHG SESQEIISST REQTDIFSEQ
430 440 450 460 470 480
KTETESMEDF QNVLKPCSLC EKRPRDGNII HGKTSHLTTC FHCARRLKKS GASCPACKKE
IQLVIKVFIA