Q5R5M7

SS
Gene name
RAF1
Protein name
RAF proto-oncogene serine/threonine-protein kinase
Names
Proto-oncogene c-RAF, cRaf, Raf-1
Species
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
KEGG Pathway
pon:100173732
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

Raf proteins are Ras-regulated serine/threonine protein kinases that control the activation of the ERK/MARK cascade, and consists of three isoforms, A-Raf, B-Raf, and Raf-1 (C-Raf), which exhibit a high degree of homology within three conserved regions (CR) known as CR1, CR2, and CR3. In studies with human RAF1, when the catalytic domain of Raf-1 is expressed alone, it exhibits a constutive activity. Raf-1 is regulated by an N-terminal autoinhibitory domain including RBD and CRD domains. The autoinhibitory region blocks the catalytic kinase domain (CR3) and the autoinhibition is interrupted by the interaction with Ras.

Autoinhibitory domains (AIDs)

51-186 (RBD domain and CRD domain) SS

Target domain

349-609 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

485-510 (Activation loop from InterPro)

Target domain

349-609 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q5R5M7

Entry ID Method Resolution Chain Position Source
AF-Q5R5M7-F1 Predicted AlphaFoldDB

No variants for Q5R5M7

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q5R5M7

No associated diseases with Q5R5M7

6 regional properties for Q5R5M7

Type Name Position InterPro Accession
domain Protein kinase domain 349 - 609 IPR000719
domain Serine-threonine/tyrosine-protein kinase, catalytic domain 351 - 605 IPR001245
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 138 - 186 IPR002219
domain Raf-like Ras-binding 56 - 131 IPR003116
active_site Serine/threonine-protein kinase, active site 464 - 476 IPR008271
binding_site Protein kinase, ATP binding site 355 - 375 IPR017441

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
  • Mitochondrion
  • Nucleus
  • Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes
  • Phosphorylation at Ser-259 impairs its membrane accumulation
  • Recruited to the cell membrane by the active Ras protein
  • Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization
  • Retinoic acid-induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

4 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

5 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
metal ion binding Binding to a metal ion.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

1 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MEHIQGAWKT ISNGFGFKDA VFDGSSCISP TIVQQFGYQR RASDDGKLTD PSKTSNTIRV
70 80 90 100 110 120
FLPNKQRTVV NVRNGMSLHD CLMKALKVRG LQPECCAVFR LLHEHKGKKA RLDWNTDAAS
130 140 150 160 170 180
LIGEELQVDF LDHVPLTTHN FARKTFLKLA FCDICQKFLL NGFRCQTCGY KFHEHCSTKV
190 200 210 220 230 240
PTMCVDWSNI RQLLLFPNST IGDSGVPALP SLTMRRMRES VSRMPVSSQH RYSTPHAFTF
250 260 270 280 290 300
NTSSPSSEGS LSQRQRSTST PNVHMVSTTL PVDSRMIEDA IRSHSESASP SALSSSPNNL
310 320 330 340 350 360
SPTGWSQPKT PVPAQRERAP VSGTQEKNKI RPRGQRDSSY YWEIEASEVM LSTRIGSGSF
370 380 390 400 410 420
GTVYKGKWHG DVAVKILKVV DPTPEQFQAF RNEVAVLRKT RHVNILLFMG YMTKDNLAIV
430 440 450 460 470 480
TQWCEGSSLY KHLHVQETKF QMFQLIDIAR QTAQGMDYLH AKNIIHRDMK SNNIFLHEGL
490 500 510 520 530 540
TVKIGDFGLA TVKSRWSGSQ QVEQPTGSVL WMAPEVIRMQ DNNPFSFQSD VYSYGIVLYE
550 560 570 580 590 600
LMTGELPYSH INNRDQIIFM VGRGYASPDL SKLYKNCPKA MKRLVADCVK KVKEERPLFP
610 620 630 640
QILSSIELLQ HSLPKINRSA SEPSLHRAAH TEDINACTLT TSPRLPVF