Q5EAB2

PR
Gene name
CDK9
Protein name
Cyclin-dependent kinase 9
Names
Cell division protein kinase 9
Species
Bos taurus (Bovine)
KEGG Pathway
bta:520580
EC number
2.7.11.22: Protein-serine/threonine kinases
Protein Class

Descriptions

The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.

Autoinhibitory domains (AIDs)

292-372 (Predicted disordered autoinhibitory region) PR

Target domain

Relief mechanism

Assay

cis-regPred

Accessory elements

166-193 (Activation loop from InterPro)

Target domain

19-315 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q5EAB2

Entry ID Method Resolution Chain Position Source
AF-Q5EAB2-F1 Predicted AlphaFoldDB

99 variants for Q5EAB2

Variant ID(s) Position Change Description Diseaes Association Provenance
rs457647393 2 A>G No EVA
rs439650930 5 Y>* No EVA
rs470945379 5 Y>D No EVA
rs470945379 5 Y>N No EVA
rs459768343 7 S>P No EVA
rs473477313 8 V>G No EVA
rs442146749 9 E>G No EVA
rs461100043 10 C>G No EVA
rs481266759 13 C>F No EVA
rs449862866 13 C>W No EVA
rs463501375 16 V>G No EVA
rs476779622 18 K>Q No EVA
rs445586260 20 E>* No EVA
rs434473857 21 K>N No EVA
rs448170359 22 L>F No EVA
rs468707024 22 L>P No EVA
rs437403855 23 A>D No EVA
rs467865870 33 V>G No EVA
rs481594107 50 V>A No EVA
rs448968392 53 E>G No EVA
rs438982744 66 E>* No EVA
rs452618258 66 E>A No EVA
rs472859515 66 E>D No EVA
rs461271661 91 P>L No EVA
rs447454603 91 P>T No EVA
rs481146943 122 T>P No EVA
rs449577950 135 N>K No EVA
rs466286348 152 A>P No EVA
rs455118961 157 I>N No EVA
rs475279524 160 D>N No EVA
rs439687943 174 F>I No EVA
rs459862508 176 L>P No EVA
rs440969035 178 K>R No EVA
rs461137246 179 N>K No EVA
rs481111843 180 S>C No EVA
rs443535372 180 S>N No EVA
rs463139349 183 N>H No EVA
rs476816765 183 N>T No EVA
rs465903213 187 N>K No EVA
rs445658555 187 N>T No EVA
rs479587371 189 V>E No EVA
rs448653847 190 V>G No EVA
rs468813949 191 T>P No EVA
rs437443347 193 W>C No EVA
rs457638300 196 P>T No EVA
rs464522711 199 L>R No EVA
rs433190962 201 L>V No EVA
rs460826386 203 E>G No EVA
rs480981143 204 R>G No EVA
rs449183806 204 R>Q No EVA
rs469252324 205 D>G No EVA
rs438057571 206 Y>S No EVA
rs432996842 208 P>S No EVA
rs453109119 210 I>L No EVA
rs455323592 216 G>W No EVA
rs475402169 217 C>S No EVA
rs444104401 222 M>L No EVA
rs451310536 224 T>A No EVA
rs451310536 224 T>P No EVA
rs471991082 224 T>S No EVA
rs440572807 228 I>L No EVA
rs460790059 229 M>R No EVA
rs480714927 234 E>Q No EVA
rs443133414 236 H>Q No EVA
rs462739198 237 Q>K No EVA
rs445270031 241 I>T No EVA
rs479190202 243 Q>R No EVA
rs446596512 245 C>R No EVA
rs466836735 247 S>P No EVA
rs435432139 248 I>L No EVA
rs455638151 250 P>H No EVA
rs468936376 251 E>G No EVA
rs433261891 255 N>H No EVA
rs473394353 258 K>Q No EVA
rs472183337 268 V>G No EVA
rs434518646 275 V>G No EVA
rs455878868 276 K>E No EVA
rs454592853 309 A>D No EVA
rs443488727 311 N>T No EVA
rs463360369 318 D>A No EVA
rs470611012 332 L>R No EVA
rs439268310 336 F>C No EVA
rs459404416 337 E>A No EVA
rs448661485 350 T>P No EVA
rs462349394 357 S>R No EVA
rs482515490 358 R>W No EVA
rs464550167 361 A>S No EVA
rs433201404 362 T>P No EVA
rs446862305 363 T>P No EVA
rs446862305 363 T>S No EVA
rs466868829 364 N>T No EVA
rs435661342 365 Q>* No EVA
rs454529275 367 E>G No EVA
rs437076533 368 F>L No EVA
rs474656360 368 F>V No EVA
rs457205255 369 E>G No EVA
rs470581761 371 V>F No EVA
rs439152949 372 F>L No EVA
rs459353433 373 F>L No EVA

No associated diseases with Q5EAB2

3 regional properties for Q5EAB2

Type Name Position InterPro Accession
domain Protein kinase domain 19 - 315 IPR000719
active_site Serine/threonine-protein kinase, active site 145 - 157 IPR008271
binding_site Protein kinase, ATP binding site 25 - 48 IPR017441

Functions

Description
EC Number 2.7.11.22 Protein-serine/threonine kinases
Subcellular Localization
  • Nucleus
  • Cytoplasm
  • Nucleus, PML body
  • Accumulates on chromatin in response to replication stress
  • Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm
  • The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent
  • Associates with PML body when acetylated (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

7 GO annotations of cellular component

Name Definition
cyclin/CDK positive transcription elongation factor complex A transcription elongation factor complex that facilitates the transition from abortive to productive elongation by phosphorylating the CTD domain of the large subunit of DNA-directed RNA polymerase II, holoenzyme. Contains a cyclin and a cyclin-dependent protein kinase catalytic subunit.
cytoplasmic ribonucleoprotein granule A ribonucleoprotein granule located in the cytoplasm.
mediator complex A protein complex that interacts with the carboxy-terminal domain of the largest subunit of RNA polymerase II and plays an active role in transducing the signal from a transcription factor to the transcriptional machinery. The mediator complex is required for activation of transcription of most protein-coding genes, but can also act as a transcriptional corepressor. The Saccharomyces complex contains several identifiable subcomplexes: a head domain comprising Srb2, -4, and -5, Med6, -8, and -11, and Rox3 proteins; a middle domain comprising Med1, -4, and -7, Nut1 and -2, Cse2, Rgr1, Soh1, and Srb7 proteins; a tail consisting of Gal11p, Med2p, Pgd1p, and Sin4p; and a regulatory subcomplex comprising Ssn2, -3, and -8, and Srb8 proteins. Metazoan mediator complexes have similar modular structures and include homologs of yeast Srb and Med proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.
P-TEFb complex A dimeric positive transcription elongation factor complex b that comprises a cyclin-dependent kinase containing the catalytic subunit, Cdk9, and a regulatory subunit, cyclin T.
PML body A class of nuclear body; they react against SP100 auto-antibodies (PML, promyelocytic leukemia); cells typically contain 10-30 PML bodies per nucleus; alterations in the localization of PML bodies occurs after viral infection.
transcription elongation factor complex Any protein complex that interacts with RNA polymerase II to increase (positive transcription elongation factor) or reduce (negative transcription elongation factor) the rate of transcription elongation.

10 GO annotations of molecular function

Name Definition
7SK snRNA binding Binding to a 7SK small nuclear RNA (7SK snRNA).
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chromatin binding Binding to chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.
cyclin-dependent protein serine/threonine kinase activity Cyclin-dependent catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein kinase binding Binding to a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
RNA polymerase II cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.
RNA polymerase II CTD heptapeptide repeat kinase activity Catalysis of the reaction: ATP + RNA polymerase II large subunit CTD heptapeptide repeat (YSPTSPS) = ADP + H+ + phosphorylated RNA polymerase II.
transcription coactivator binding Binding to a transcription coactivator, a protein involved in positive regulation of transcription via protein-protein interactions with transcription factors and other proteins that positively regulate transcription. Transcription coactivators do not bind DNA directly, but rather mediate protein-protein interactions between activating transcription factors and the basal transcription machinery.

15 GO annotations of biological process

Name Definition
cellular response to cytokine stimulus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cytokine stimulus.
DNA repair The process of restoring DNA after damage. Genomes are subject to damage by chemical and physical agents in the environment (e.g. UV and ionizing radiations, chemical mutagens, fungal and bacterial toxins, etc.) and by free radicals or alkylating agents endogenously generated in metabolism. DNA is also damaged because of errors during its replication. A variety of different DNA repair pathways have been reported that include direct reversal, base excision repair, nucleotide excision repair, photoreactivation, bypass, double-strand break repair pathway, and mismatch repair pathway.
phosphorylation of RNA polymerase II C-terminal domain serine 2 residues involved in positive regulation of transcription elongation from RNA polymerase II promoter Any phosphorylation of RNA polymerase II C-terminal domain serine 2 residues that is involved in positive regulation of transcription elongation from RNA polymerase II promoter.
phosphorylation of RNA polymerase II C-terminal domain serine 5 residues involved in positive regulation of transcription elongation from RNA polymerase II promoter Any phosphorylation of RNA polymerase II C-terminal domain serine 5 residues that is involved in positive regulation of transcription elongation from RNA polymerase II promoter.
positive regulation by host of viral transcription Any process in which a host organism activates or increases the frequency, rate or extent of viral transcription, the synthesis of either RNA on a template of DNA or DNA on a template of RNA.
positive regulation of mRNA 3'-UTR binding Any process that activates or increases the frequency, rate or extent of mRNA 3'-UTR binding.
positive regulation of protein localization to chromatin Any process that activates or increases the frequency, rate or extent of protein localization to chromatin.
positive regulation of transcription by RNA polymerase II Any process that activates or increases the frequency, rate or extent of transcription from an RNA polymerase II promoter.
protein phosphorylation The process of introducing a phosphate group on to a protein.
regulation of DNA repair Any process that modulates the frequency, rate or extent of DNA repair.
regulation of histone modification Any process that modulates the frequency, rate or extent of the covalent alteration of a histone.
regulation of mRNA 3'-end processing Any process that modulates the frequency, rate or extent of mRNA 3'-end processing, any process involved in forming the mature 3' end of an mRNA molecule.
regulation of muscle cell differentiation Any process that modulates the frequency, rate or extent of muscle cell differentiation.
replication fork processing The process in which a DNA replication fork that has stalled is restored to a functional state and replication is restarted. The stalling may be due to DNA damage, DNA secondary structure, bound proteins, dNTP shortage, or other causes.
transcription by RNA polymerase II The synthesis of RNA from a DNA template by RNA polymerase II (RNAP II), originating at an RNA polymerase II promoter. Includes transcription of messenger RNA (mRNA) and certain small nuclear RNAs (snRNAs).

8 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q2TBL8 CDK10 Cyclin-dependent kinase 10 Bos taurus (Bovine) PR
Q5ZKN1 CDK9 Cyclin-dependent kinase 9 Gallus gallus (Chicken) PR
P50750 CDK9 Cyclin-dependent kinase 9 Homo sapiens (Human) PR
Q99J95 Cdk9 Cyclin-dependent kinase 9 Mus musculus (Mouse) PR
Q641Z4 Cdk9 Cyclin-dependent kinase 9 Rattus norvegicus (Rat) PR
F4I114 At1g09600 Probable serine/threonine-protein kinase At1g09600 Arabidopsis thaliana (Mouse-ear cress) PR
Q9ZVM9 At1g54610 Probable serine/threonine-protein kinase At1g54610 Arabidopsis thaliana (Mouse-ear cress) PR
Q6GLD8 cdk9 Cyclin-dependent kinase 9 Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) PR
10 20 30 40 50 60
MAKQYDSVEC PFCDEVTKYE KLAKIGQGTF GEVFKAKHRK TGQKVALKKV LMENEKEGFP
70 80 90 100 110 120
ITALREIKIL QLLKHENVVN LIEICRTKAS PYNRCKGSIY LVFDFCEHDL AGLLSNVLVK
130 140 150 160 170 180
FTLSEIKRVM QMLLNGLYYI HRNKILHRDM KAANVLITRD GVLKLADFGL ARAFSLAKNS
190 200 210 220 230 240
QPNRYTNRVV TLWYRPPELL LGERDYGPPI DLWGAGCIMA EMWTRSPIMQ GNTEQHQLAL
250 260 270 280 290 300
ISQLCGSITP EVWPNVDKYE LFEKVELVKG QKRKVKDRLK AYVRDPYALD LIDKLLVLDP
310 320 330 340 350 360
AQRIDSDDAL NHDFFWSDPM PSDLKGMLST HLTSMFEYLA PPRRKGSQIT QQSTNQSRNP
370
ATTNQTEFER VF