Q5E9I6

SS
Gene name
ARF3
Protein name
ADP-ribosylation factor 3
Names
Species
Bos taurus (Bovine)
KEGG Pathway
bta:510994
EC number
Protein Class

Descriptions

Small GTPases couple their GDP/GTP structural cycle to cytosol/membrane alternation to function as versatile molecular switches in the cell. Membrane localization of their active, GTP-bound form is pivotal to their ability to propagate information, and this requires their post-translational modification by lipids.<br>Arf GTPases are modified by a myristate attached to their N-terminus, which is shielded by intramolecular interactions in their inactive state. The myristoylated N-terminus of Arf is autoinhibitory in solution and is displaced by membranes, priming Arf GTPases for activation by their GEFs. Replacement of the N-terminal myristate by a 6xHis-tag preserves autoinhibition, representing that membranes unlock the N-terminal region to facilitate subsequent activation.

Autoinhibitory domains (AIDs)

3-16 (N-terminal helix) SS

Target domain

16-141 (Small GTP-binding protein domain)

Relief mechanism

Ligand binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q5E9I6

Entry ID Method Resolution Chain Position Source
AF-Q5E9I6-F1 Predicted AlphaFoldDB

No variants for Q5E9I6

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q5E9I6

No associated diseases with Q5E9I6

1 regional properties for Q5E9I6

Type Name Position InterPro Accession
domain High mobility group box domain 48 - 118 IPR009071

Functions

Description
EC Number
Subcellular Localization
  • Golgi apparatus
  • Cytoplasm, perinuclear region
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
Golgi apparatus A membrane-bound cytoplasmic organelle of the endomembrane system that further processes the core oligosaccharides (e.g. N-glycans) added to proteins in the endoplasmic reticulum and packages them into membrane-bound vesicles. The Golgi apparatus operates at the intersection of the secretory, lysosomal, and endocytic pathways.
perinuclear region of cytoplasm Cytoplasm situated near, or occurring around, the nucleus.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

2 GO annotations of molecular function

Name Definition
GTP binding Binding to GTP, guanosine triphosphate.
GTPase activity Catalysis of the reaction

2 GO annotations of biological process

Name Definition
intracellular protein transport The directed movement of proteins in a cell, including the movement of proteins between specific compartments or structures within a cell, such as organelles of a eukaryotic cell.
vesicle-mediated transport A cellular transport process in which transported substances are moved in membrane-bounded vesicles; transported substances are enclosed in the vesicle lumen or located in the vesicle membrane. The process begins with a step that directs a substance to the forming vesicle, and includes vesicle budding and coating. Vesicles are then targeted to, and fuse with, an acceptor membrane.

19 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q0VC18 ARL4D ADP-ribosylation factor-like protein 4D Bos taurus (Bovine) PR
Q3SZF2 ARF4 ADP-ribosylation factor 4 Bos taurus (Bovine) SS
P84081 ARF2 ADP-ribosylation factor 2 Bos taurus (Bovine) SS
P84080 ARF1 ADP-ribosylation factor 1 Bos taurus (Bovine) SS
Q2KJ96 ARL5A ADP-ribosylation factor-like protein 5A Bos taurus (Bovine) SS
P61209 Arf1 ADP-ribosylation factor 1 Drosophila melanogaster (Fruit fly) SS
P84077 ARF1 ADP-ribosylation factor 1 Homo sapiens (Human) EV
P61204 ARF3 ADP-ribosylation factor 3 Homo sapiens (Human) EV
P49076 ARF1 ADP-ribosylation factor Zea mays (Maize) SS
P84078 Arf1 ADP-ribosylation factor 1 Mus musculus (Mouse) SS
P61205 Arf3 ADP-ribosylation factor 3 Mus musculus (Mouse) SS
P84079 Arf1 ADP-ribosylation factor 1 Rattus norvegicus (Rat) SS
P61206 Arf3 ADP-ribosylation factor 3 Rattus norvegicus (Rat) SS
P51823 ARF ADP-ribosylation factor 2 Oryza sativa subsp. japonica (Rice) SS
Q06396 Os01g0813400 ADP-ribosylation factor 1 Oryza sativa subsp. japonica (Rice) SS
Q10943 arf-1.2 ADP-ribosylation factor 1-like 2 Caenorhabditis elegans SS
P0DH91 ARF2-B ADP-ribosylation factor 2-B Arabidopsis thaliana (Mouse-ear cress) SS
P36397 ARF1 ADP-ribosylation factor 1 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LQC8 ARF2-A ADP-ribosylation factor 2-A Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
70 80 90 100 110 120
ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV
130 140 150 160 170 180
LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK
K