Descriptions
Ngef acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases. Ngef has the autoinhibitory helix which can regulate protein by phosphorylation. The substitution of Tyr 179 with Glu (Y179E) within the putative autoinhibitory helix activated Ngef to the same extent as removing this region by truncation, and likely Ngef does.
Autoinhibitory domains (AIDs)
Target domain |
264-448 (DH domain) |
Relief mechanism |
PTM |
Assay |
|
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q5BKC9
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q5BKC9-F1 | Predicted | AlphaFoldDB |
No variants for Q5BKC9
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q5BKC9 | |||||
No associated diseases with Q5BKC9
5 regional properties for Q5BKC9
5 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| glutamatergic synapse | A synapse that uses glutamate as a neurotransmitter. |
| growth cone | The migrating motile tip of a growing neuron projection, where actin accumulates, and the actin cytoskeleton is the most dynamic. |
| membrane | A lipid bilayer along with all the proteins and protein complexes embedded in it and attached to it. |
| postsynapse | The part of a synapse that is part of the post-synaptic cell. |
2 GO annotations of molecular function
| Name | Definition |
|---|---|
| ephrin receptor binding | Binding to an ephrin receptor. |
| guanyl-nucleotide exchange factor activity | Stimulates the exchange of GDP to GTP on a signaling GTPase, changing its conformation to its active form. Guanine nucleotide exchange factors (GEFs) act by stimulating the release of guanosine diphosphate (GDP) to allow binding of guanosine triphosphate (GTP), which is more abundant in the cell under normal cellular physiological conditions. |
6 GO annotations of biological process
| Name | Definition |
|---|---|
| cell differentiation | The cellular developmental process in which a relatively unspecialized cell, e.g. embryonic or regenerative cell, acquires specialized structural and/or functional features that characterize a specific cell. Differentiation includes the processes involved in commitment of a cell to a specific fate and its subsequent development to the mature state. |
| ephrin receptor signaling pathway | The series of molecular signals initiated by ephrin binding to its receptor, and ending with the regulation of a downstream cellular process, e.g. transcription. |
| negative regulation of dendritic spine morphogenesis | Any process that decreases the rate, frequency, or extent of dendritic spine morphogenesis, the process in which the anatomical structures of a dendritic spine are generated and organized. A dendritic spine is a protrusion from a dendrite and a specialized subcellular compartment involved in synaptic transmission. |
| nervous system development | The process whose specific outcome is the progression of nervous tissue over time, from its formation to its mature state. |
| regulation of GTPase activity | Any process that modulates the rate of GTP hydrolysis by a GTPase. |
| regulation of synapse pruning | Any process that modulates the frequency, rate or extent of synapse pruning. |
12 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q12774 | ARHGEF5 | Rho guanine nucleotide exchange factor 5 | Homo sapiens (Human) | EV |
| Q8IW93 | ARHGEF19 | Rho guanine nucleotide exchange factor 19 | Homo sapiens (Human) | SS |
| Q96DR7 | ARHGEF26 | Rho guanine nucleotide exchange factor 26 | Homo sapiens (Human) | SS |
| O94989 | ARHGEF15 | Rho guanine nucleotide exchange factor 15 | Homo sapiens (Human) | SS |
| A5YM69 | ARHGEF35 | Rho guanine nucleotide exchange factor 35 | Homo sapiens (Human) | PR |
| Q5VV41 | ARHGEF16 | Rho guanine nucleotide exchange factor 16 | Homo sapiens (Human) | PR |
| Q8N5V2 | NGEF | Ephexin-1 | Homo sapiens (Human) | EV |
| Q8BWA8 | Arhgef19 | Rho guanine nucleotide exchange factor 19 | Mus musculus (Mouse) | PR |
| Q3U5C8 | Arhgef16 | Rho guanine nucleotide exchange factor 16 | Mus musculus (Mouse) | PR |
| E9Q7D5 | Arhgef5 | Rho guanine nucleotide exchange factor 5 | Mus musculus (Mouse) | SS |
| Q5FWH6 | Arhgef15 | Rho guanine nucleotide exchange factor 15 | Mus musculus (Mouse) | SS |
| Q8CHT1 | Ngef | Ephexin-1 | Mus musculus (Mouse) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| METKNSEDQG | KPQRKSVSSL | WKSKHGPAEM | RPELPPETAK | ETQNEEPRCL | IPIQRNSLFN |
| 70 | 80 | 90 | 100 | 110 | 120 |
| RAMRHKHKAR | SMSERRANDQ | AGDLPETRKS | VNEPLAFNLP | QGRLPPWRTP | AQRGPGAQEA |
| 130 | 140 | 150 | 160 | 170 | 180 |
| SESSSTPGNG | TTPEECPALT | DSPTTLTEAL | QMIHPIPADS | WRNLIEQIGL | LYQEYRDKST |
| 190 | 200 | 210 | 220 | 230 | 240 |
| LQEIETRRQQ | DAEIQGNSDG | SQAGEDNAEE | EEEEEEEPAS | PPERRALPQI | CLLSNPHSRF |
| 250 | 260 | 270 | 280 | 290 | 300 |
| NLWQDLPEIQ | SSGVLDILQP | EETKLQEAMF | ELVTSEASYY | KSLNLLVSHF | MENERLKKIL |
| 310 | 320 | 330 | 340 | 350 | 360 |
| HPSEAHILFS | NVLDVMAVSE | RFLLELEHRM | EENIVISDVC | DIVYRYAADH | FSVYITYVSN |
| 370 | 380 | 390 | 400 | 410 | 420 |
| QTYQERTYKQ | LLQEKTAFRE | LIAQLELDPK | CKGLPLSSFL | ILPFQRITRL | KLLVQNILKR |
| 430 | 440 | 450 | 460 | 470 | 480 |
| VEEGSEREGT | ALDAHKELEM | VVKACNEGVR | KMSRTEQMIS | IQKKMEFKIK | SVPIISHSRW |
| 490 | 500 | 510 | 520 | 530 | 540 |
| LLKQGELQQM | SGPKTSRTLR | TKKLFREIYL | FLFNDLLVIC | RQIPGDKYQV | FDSAPRGLLR |
| 550 | 560 | 570 | 580 | 590 | 600 |
| VEELEDQGQT | LANVFILRLL | ENADDREATY | MLKASSQSEM | KRWMTSLAPN | RRTKFVSFTS |
| 610 | 620 | 630 | 640 | 650 | 660 |
| RLLDCPQVQC | VHPYVAQQPD | ELTLELADIL | NILEKTEDGW | IFGERLHDQE | RGWFPSSMTE |
| 670 | 680 | 690 | 700 | ||
| EILNPKIRSQ | NLKECFRVHK | MEDPQRSQNK | DRRKLGSRNR | Q |