Q4R6G4

SS
Gene name
SLC9A3R1 (NHERF, NHERF1, QtsA-18078)
Protein name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
Names
NHERF-1, Ezrin-radixin-moesin-binding phosphoprotein 50, EBP50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1
Species
Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
KEGG Pathway
mcf:101926883
EC number
Protein Class

Descriptions

The mammalian Na+/H+ exchange regulatory factor 1 (NHERF1) is a multidomain scaffolding protein essential for regulating the intracellular trafficking and macromolecular assembly of transmembrane ion channels and receptors. NHERF1 is held in an autoinhibited state through intramolecular interactions between PDZ2 and the CT domain that also includes a C-terminal PDZ-binding motif (-SNL). A central feature of NHERF1 is its binding to ezrin and to other ezrin-radixin-moesin (ERM) proteins, forming a communication bridge between plasma membrane proteins and the actin cytoskeleton ezrin modulates the PDZ domains of NHERF1 to assemble multiprotein complexes in a cooperative fashion. Upon binding to ezrin, the intramolecular domain-domain interaction is released.

Autoinhibitory domains (AIDs)

242-329 (CT domain, EB domain, and PDZ-binding motif) SS

Target domain

14-94 (PDZ1 domain); 150-240 (PDZ2 domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q4R6G4

Entry ID Method Resolution Chain Position Source
AF-Q4R6G4-F1 Predicted AlphaFoldDB

No variants for Q4R6G4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q4R6G4

No associated diseases with Q4R6G4

3 regional properties for Q4R6G4

Type Name Position InterPro Accession
domain PDZ domain 14 - 94 IPR001478-1
domain PDZ domain 154 - 234 IPR001478-2
domain EBP50, C-terminal 235 - 358 IPR015098

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Apical cell membrane
  • Endomembrane system ; Peripheral membrane protein
  • Cell projection, filopodium
  • Cell projection, ruffle
  • Cell projection, microvillus
  • Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner
  • Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast
  • Present in lipid rafts of T-cells
  • Colocalizes with CFTR at the midpiece of sperm tail (By similarity)
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

10 GO annotations of cellular component

Name Definition
apical plasma membrane The region of the plasma membrane located at the apical end of the cell.
cell periphery The part of a cell encompassing the cell cortex, the plasma membrane, and any external encapsulating structures.
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
endomembrane system A collection of membranous structures involved in transport within the cell. The main components of the endomembrane system are endoplasmic reticulum, Golgi bodies, vesicles, cell membrane and nuclear envelope. Members of the endomembrane system pass materials through each other or though the use of vesicles.
filopodium Thin, stiff, actin-based protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal or dendritic growth cone, or a dendritic shaft.
membrane A lipid bilayer along with all the proteins and protein complexes embedded in it an attached to it.
microvillus Thin cylindrical membrane-covered projections on the surface of an animal cell containing a core bundle of actin filaments. Present in especially large numbers on the absorptive surface of intestinal cells.
microvillus membrane The portion of the plasma membrane surrounding a microvillus.
ruffle Projection at the leading edge of a crawling cell; the protrusions are supported by a microfilament meshwork.
sperm midpiece The highly organized segment of the sperm flagellum which begins at the connecting piece and is characterized by the presence of 9 outer dense fibers (ODFs) that lie outside each of the 9 outer axonemal microtubule doublets and by a sheath of mitochondria that encloses the ODFs and the axoneme; the midpiece terminates about one-fourth of the way down the sperm flagellum at the annulus, which marks the beginning of the principal piece.

2 GO annotations of molecular function

Name Definition
chloride channel regulator activity Binds to and modulates the activity of a chloride channel.
molecular adaptor activity The binding activity of a molecule that brings together two or more molecules through a selective, non-covalent, often stoichiometric interaction, permitting those molecules to function in a coordinated way.

10 GO annotations of biological process

Name Definition
bile acid secretion The regulated release of bile acid, composed of any of a group of steroid carboxylic acids occurring in bile, by a cell or a tissue.
glutathione transport The directed movement of glutathione, the tripeptide glutamylcysteinylglycine, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
negative regulation of cell motility Any process that stops, prevents, or reduces the frequency, rate or extent of cell motility.
negative regulation of phosphatidylinositol 3-kinase signaling Any process that stops, prevents, or reduces the frequency, rate or extent of signal transduction mediated by the phosphatidylinositol 3-kinase cascade.
negative regulation of platelet-derived growth factor receptor signaling pathway Any process that stops, prevents, or reduces the frequency, rate or extent of the platelet-derived growth factor receptor signaling pathway.
negative regulation of protein kinase B signaling Any process that stops, prevents, or reduces the frequency, rate or extent of protein kinase B signaling, a series of reactions mediated by the intracellular serine/threonine kinase protein kinase B.
regulation of protein kinase activity Any process that modulates the frequency, rate or extent of protein kinase activity.
renal absorption A renal system process in which water, ions, glucose and proteins are taken up from the collecting ducts, glomerulus and proximal and distal loops of the nephron. In non-mammalian species, absorption may occur in related structures (e.g. protein absorption is observed in nephrocytes in Drosophila, see PMID:23264686).
renal phosphate ion absorption A renal system process in which phosphate ions are taken up from the collecting ducts and proximal and distal loops of the nephron. In non-mammalian species, absorption may occur in related structures.
Wnt signaling pathway The series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell and ending with a change in cell state.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSADAAAGAP LPRLCCLEKG PNGYGFHLHG EKGKLGQYIR LVEPGSPAEK AGLLAGDRLV
70 80 90 100 110 120
EVNGENVEKE THQQVVSRIR AALNAVRLLV VDPETDEQLQ KLGVQVREEL LRAQETPGQA
130 140 150 160 170 180
EPAAAAEAQG AGNENEPREA DKSHPEQRKL RPRLCTMKKG PSGYGFNLHS DKSKPGQFIR
190 200 210 220 230 240
SVDPDSPAEA SGLRAQDRIV EVNGVCMEGK QHGDVVSAIR AGGDETKLLV VDRETDEFFK
250 260 270 280 290 300
KCKVTPSQEH LNGPLPEPFT NGEIQKENSR EALAEAASES PRPTLVRSAS SDTSEELNSQ
310 320 330 340 350
DSPPKQDSTA PSSTSSSDPI LDFNISLAMA KERAHQKRSS KRAPQMDWSK KNELFSNL