Q4JF28

SS
Gene name
IRF3
Protein name
Interferon regulatory factor 3
Names
IRF-3
Species
Bos taurus (Bovine)
KEGG Pathway
bta:516979
EC number
Protein Class

Descriptions

IRF3 is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses and is present in an inactive cytoplasmic form. Human IRF3 (Q14653) contains a C-terminal region containing phosphorylation sites. Phosphorylation of the sites converts IRF3 into a constitutively active form. The C-terminal autoinhibitory region intramolecularly interact with N-terminal region overlapping with an atypical transactivation domain.

Autoinhibitory domains (AIDs)

376-417 (C-terminal autoinhibitory region) SS

Target domain

197-376 (IFN association domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q4JF28

Entry ID Method Resolution Chain Position Source
AF-Q4JF28-F1 Predicted AlphaFoldDB

222 variants for Q4JF28

Variant ID(s) Position Change Description Diseaes Association Provenance
rs471186362 8 I>M No Ensembl
rs444527760 8 I>R No Ensembl
rs440734683 11 W>C No Ensembl
rs473654788 14 S>F No Ensembl
rs473654788 14 S>Y No Ensembl
rs469003634 17 D>G No Ensembl
rs435939956 17 D>H No Ensembl
rs435939956 17 D>Y No Ensembl
rs456999663 19 G>A No Ensembl
rs432110717 20 E>A No Ensembl
rs446564060 24 V>G No Ensembl
rs434551145 25 A>S No Ensembl
rs467476527 27 L>P No Ensembl
rs449114000 29 E>A No Ensembl
rs481205824 30 S>R No Ensembl
rs450973387 32 T>A No Ensembl
rs450973387 32 T>P No Ensembl
rs459058418 33 R>P No Ensembl
rs473606098 34 F>L No Ensembl
rs461623444 34 F>S No Ensembl
rs475440035 35 R>P No Ensembl
rs431967132 38 W>L No Ensembl
rs471426591 39 K>Q No Ensembl
rs452911707 40 H>D No Ensembl
rs452911707 40 H>N No Ensembl
rs434514346 41 G>R No Ensembl
rs437108652 42 L>F No Ensembl
rs449075509 42 L>V No Ensembl
rs469395541 43 R>G No Ensembl
rs450758925 44 Q>P No Ensembl
rs458946689 45 D>E No Ensembl
rs477445399 45 D>G No Ensembl
rs446997976 46 A>G No Ensembl
rs443134320 47 Q>H No Ensembl
rs461588662 47 Q>P No Ensembl
rs476064654 48 Q>P No Ensembl
rs476064654 48 Q>R No Ensembl
rs464065458 49 E>G No Ensembl
rs438355704 50 D>G No Ensembl
rs471419928 51 F>V No Ensembl
rs434477130 52 G>C No Ensembl
rs434477130 52 G>R No Ensembl
rs455418704 54 F>L No Ensembl
rs470023519 55 Q>P No Ensembl
rs463156259 61 S>R No Ensembl
rs471245348 63 A>G No Ensembl
rs452851366 65 T>N No Ensembl
rs433528728 67 G>E No Ensembl
rs473028191 72 D>G No Ensembl
rs454604597 89 V>G No Ensembl
rs1114509873 91 R>C No Ensembl
rs469349502 136 E>* No Ensembl
rs457278220 137 D>E No Ensembl
rs432366085 140 E>G No Ensembl
rs717915109 141 K>N No Ensembl
rs465428358 147 D>A No Ensembl
rs446997939 147 D>E No Ensembl
rs480047377 160 S>F No Ensembl
rs520313532 162 K>N No Ensembl
rs1116702921 163 P>A No Ensembl
rs1118251781 166 F>L No Ensembl
rs1118263930 168 Q>L No Ensembl
rs468039177 169 S>G No Ensembl
rs436119778 170 P>H No Ensembl
rs482532463 170 P>T No Ensembl
rs464025449 172 S>A No Ensembl
rs477627801 183 L>I No Ensembl
rs477627801 183 L>V No Ensembl
rs459260419 185 E>D No Ensembl
rs440900785 186 N>T No Ensembl
rs473996538 187 P>A No Ensembl
rs462000884 188 L>M No Ensembl
rs443398532 193 A>G No Ensembl
rs476512649 194 N>D No Ensembl
rs476512649 194 N>H No Ensembl
rs451643775 195 E>A No Ensembl
rs447192038 198 W>G No Ensembl
rs480298834 199 E>A No Ensembl
rs468276086 199 E>D No Ensembl
rs480298834 199 E>G No Ensembl
rs482808361 200 F>C No Ensembl
rs449738379 200 F>I No Ensembl
rs449738379 200 F>L No Ensembl
rs449738379 200 F>V No Ensembl
rs478866145 201 E>G No Ensembl
rs439431760 201 E>K No Ensembl
rs478866145 201 E>V No Ensembl
rs441167836 202 V>A No Ensembl
rs441167836 202 V>E No Ensembl
rs441167836 202 V>G No Ensembl
rs474236178 203 T>S No Ensembl
rs476603678 204 A>G No Ensembl
rs443733870 204 A>S No Ensembl
rs433356907 205 F>L No Ensembl
rs433356907 205 F>V No Ensembl
rs435161261 206 Y>* No Ensembl
rs454372581 206 Y>C No Ensembl
rs454372581 206 Y>F No Ensembl
rs466278644 206 Y>H No Ensembl
rs449764295 208 G>C No Ensembl
rs482669410 208 G>V No Ensembl
rs445828937 209 C>F No Ensembl
rs464266719 209 C>S No Ensembl
rs478827440 209 C>W No Ensembl
rs460431381 210 Q>* No Ensembl
rs441927538 211 V>F No Ensembl
rs480639800 211 V>G No Ensembl
rs441927538 211 V>I No Ensembl
rs462165297 212 F>L No Ensembl
rs443614023 213 Q>R No Ensembl
rs476468774 215 T>P No Ensembl
rs451704080 215 T>S No Ensembl
rs472643764 218 C>W No Ensembl
rs454403655 221 G>R No Ensembl
rs435936317 222 L>Q No Ensembl
rs435936317 222 L>R No Ensembl
rs467468053 225 V>G No Ensembl
rs468906341 225 V>L No Ensembl
rs437607696 226 G>V No Ensembl
rs445790334 230 G>E No Ensembl
rs433745996 231 D>A No Ensembl
rs433745996 231 D>G No Ensembl
rs466767684 233 M>V No Ensembl
rs448365043 234 L>V No Ensembl
rs462863846 235 P>A No Ensembl
rs482857189 237 Q>* No Ensembl
rs458065070 238 P>A No Ensembl
rs439706011 239 I>K No Ensembl
rs460654497 240 R>P No Ensembl
rs475273043 246 T>A No Ensembl
rs456841986 246 T>R No Ensembl
rs470616174 248 L>M No Ensembl
rs452216089 248 L>P No Ensembl
rs470616174 248 L>V No Ensembl
rs466686003 252 S>R No Ensembl
rs448144246 255 D>A No Ensembl
rs436203528 255 D>E No Ensembl
rs469222221 256 Y>* No Ensembl
rs450736164 257 V>L No Ensembl
rs482820067 258 Q>H No Ensembl
rs481828567 258 Q>K No Ensembl
rs458027910 259 R>G No Ensembl
rs446010134 260 V>G No Ensembl
rs479110882 263 C>F No Ensembl
rs475266305 269 A>G No Ensembl
rs442163966 269 A>S No Ensembl
rs470577158 271 W>R No Ensembl
rs452079358 273 A>G No Ensembl
rs439995841 274 G>R No Ensembl
rs473039196 275 Q>E No Ensembl
rs454632405 275 Q>P No Ensembl
rs454632405 275 Q>R No Ensembl
rs469247646 276 W>G No Ensembl
rs469247646 276 W>R No Ensembl
rs457072848 277 L>I No Ensembl
rs432206346 277 L>R No Ensembl
rs457072848 277 L>V No Ensembl
rs479077820 278 C>* No Ensembl
rs445972962 278 C>F No Ensembl
rs465108941 278 C>G No Ensembl
rs465108941 278 C>R No Ensembl
rs479077820 278 C>W No Ensembl
rs467010915 279 A>D No Ensembl
rs467010915 279 A>G No Ensembl
rs481664440 280 Q>H No Ensembl
rs448600767 280 Q>P No Ensembl
rs463197726 281 R>G No Ensembl
rs444648100 281 R>K No Ensembl
rs477654859 282 L>P No Ensembl
rs477654859 282 L>R No Ensembl
rs439961602 284 H>D No Ensembl
rs473060289 284 H>P No Ensembl
rs473060289 284 H>R No Ensembl
rs439961602 284 H>Y No Ensembl
rs442561317 285 C>* No Ensembl
rs454535799 285 C>G No Ensembl
rs454535799 285 C>R No Ensembl
rs475312542 286 H>P No Ensembl
rs432167356 287 V>E No Ensembl
rs432167356 287 V>G No Ensembl
rs456934709 287 V>L No Ensembl
rs433958787 288 Y>C No Ensembl
rs453116132 288 Y>D No Ensembl
rs467030755 290 A>G No Ensembl
rs448482052 291 I>V No Ensembl
rs481516234 292 G>S No Ensembl
rs477616487 293 E>D No Ensembl
rs451082041 293 E>G No Ensembl
rs459216077 294 E>G No Ensembl
rs479454197 295 L>P No Ensembl
rs440731785 295 L>V No Ensembl
rs475273909 299 C>G No Ensembl
rs438539972 302 K>E No Ensembl
rs471445289 302 K>N No Ensembl
rs434724189 307 V>G No Ensembl
rs474110693 308 P>A No Ensembl
rs436391641 317 N>H No Ensembl
rs469444856 318 L>P No Ensembl
rs432527569 321 F>L No Ensembl
rs465614401 324 D>H No Ensembl
rs447561004 327 T>P No Ensembl
rs480449228 330 E>D No Ensembl
rs462062017 331 G>V No Ensembl
rs442988817 334 R>H No Ensembl
rs442988817 334 R>L No Ensembl
rs439799855 339 T>P No Ensembl
rs439799855 339 T>S No Ensembl
rs472849202 341 W>R No Ensembl
rs454442452 342 F>L No Ensembl
rs474639860 343 C>* No Ensembl
rs435165852 343 C>F No Ensembl
rs474639860 343 C>W No Ensembl
rs437651038 346 Q>E No Ensembl
rs464266860 348 W>S No Ensembl
rs445758222 350 Q>H No Ensembl
rs480475867 351 D>A No Ensembl
rs466806425 357 R>M No Ensembl
rs447142067 372 V>L No Ensembl
rs457059922 388 D>N No Ensembl
rs1118272875 395 D>E No Ensembl
rs1118281959 395 D>H No Ensembl
rs1118279411 407 C>Y No Ensembl

No associated diseases with Q4JF28

2 regional properties for Q4JF28

Type Name Position InterPro Accession
domain Interferon regulatory factor, DNA-binding domain 1 - 112 IPR001346
domain Interferon regulatory factor-3 197 - 376 IPR019471

Functions

Description
EC Number
Subcellular Localization
  • Cytoplasm
  • Nucleus
  • Mitochondrion
  • Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect
  • When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm
  • Recruited to mitochondria via TOMM70:HSP90AA1 upon Sendai virus infection
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
mitochondrion A semiautonomous, self replicating organelle that occurs in varying numbers, shapes, and sizes in the cytoplasm of virtually all eukaryotic cells. It is notably the site of tissue respiration.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

7 GO annotations of molecular function

Name Definition
DNA-binding transcription activator activity, RNA polymerase II-specific A DNA-binding transcription factor activity that activates or increases transcription of specific gene sets transcribed by RNA polymerase II.
DNA-binding transcription factor activity, RNA polymerase II-specific A DNA-binding transcription factor activity that modulates the transcription of specific gene sets transcribed by RNA polymerase II.
DNA-binding transcription repressor activity, RNA polymerase II-specific A DNA-binding transcription factor activity that represses or decreases the transcription of specific gene sets transcribed by RNA polymerase II.
promoter-specific chromatin binding Binding to a section of chromatin that is associated with gene promoter sequences of DNA.
protein domain specific binding Binding to a specific domain of a protein.
protein homodimerization activity Binding to an identical protein to form a homodimer.
RNA polymerase II cis-regulatory region sequence-specific DNA binding Binding to a specific upstream regulatory DNA sequence (transcription factor recognition sequence or binding site) located in cis relative to the transcription start site (i.e., on the same strand of DNA) of a gene transcribed by RNA polymerase II.

15 GO annotations of biological process

Name Definition
cellular response to virus Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus from a virus.
defense response to virus Reactions triggered in response to the presence of a virus that act to protect the cell or organism.
immune system process Any process involved in the development or functioning of the immune system, an organismal system for calibrated responses to potential internal or invasive threats.
lipopolysaccharide-mediated signaling pathway The series of molecular signals initiated by the binding of a lipopolysaccharide (LPS) to a receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. Lipopolysaccharides are major components of the outer membrane of Gram-negative bacteria, making them prime targets for recognition by the immune system.
positive regulation of interferon-alpha production Any process that activates or increases the frequency, rate, or extent of interferon-alpha production.
positive regulation of interferon-beta production Any process that activates or increases the frequency, rate, or extent of interferon-beta production.
positive regulation of type I interferon production Any process that activates or increases the frequency, rate, or extent of type I interferon production. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families.
positive regulation of type I interferon-mediated signaling pathway Any process that increases the rate, frequency or extent of a type I interferon-mediated signaling pathway.
programmed necrotic cell death A necrotic cell death process that results from the activation of endogenous cellular processes, such as signaling involving death domain receptors or Toll-like receptors.
regulation of apoptotic process Any process that modulates the occurrence or rate of cell death by apoptotic process.
regulation of inflammatory response Any process that modulates the frequency, rate or extent of the inflammatory response, the immediate defensive reaction (by vertebrate tissue) to infection or injury caused by chemical or physical agents.
regulation of transcription by RNA polymerase II Any process that modulates the frequency, rate or extent of transcription mediated by RNA polymerase II.
response to exogenous dsRNA Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an exogenous double-stranded RNA stimulus.
TRIF-dependent toll-like receptor signaling pathway The series of molecular signals initiated by a ligand binding to a toll-like receptor where the TRIF adaptor mediates transduction of the signal. Toll-like receptors directly bind pattern motifs from a variety of microbial sources to initiate an innate immune response.
type I interferon signaling pathway The series of molecular signals initiated by type I interferon binding to its receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. Type I interferons include the interferon-alpha, beta, delta, episilon, zeta, kappa, tau, and omega gene families.

4 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q58DJ0 IRF5 Interferon regulatory factor 5 Bos taurus (Bovine) SS
Q14653 IRF3 Interferon regulatory factor 3 Homo sapiens (Human) EV
P70671 Irf3 Interferon regulatory factor 3 Mus musculus (Mouse) SS
Q764M6 IRF3 Interferon regulatory factor 3 Sus scrofa (Pig) SS
10 20 30 40 50 60
MGTQKPRILP WLISQLDRGE LEGVAWLGES RTRFRIPWKH GLRQDAQQED FGIFQAWAVA
70 80 90 100 110 120
SGAYTPGKDK PDLPTWKRNF RSALNRKEVL RLAEDHSKDS QDPHKIYEFV NSGVRDIPEP
130 140 150 160 170 180
DTSQDNGRHN TSDTQEDTLE KLLSDMDLSP GGPSNLTMAS EKPPQFLQSP DSDIPALCPN
190 200 210 220 230 240
SGLSENPLKQ LLANEEDWEF EVTAFYRGCQ VFQQTVFCPG GLRLVGSEAG DRMLPGQPIR
250 260 270 280 290 300
LPDPATSLTD KSVTDYVQRV LSCLGGGLAL WRAGQWLCAQ RLGHCHVYWA IGEELLPSCG
310 320 330 340 350 360
HKPDGEVPKD REGGVFNLGP FITDLITFIE GSRRSPLYTL WFCVGQSWPQ DQPWIKRLVM
370 380 390 400 410
VKVVPMCLRV LVDIARQGGA SSLENTVDLH ISNSDPLSLT PDQYMACLQD LAEDMDF