Q48F24
Gene name |
wspR (PSPPH_3875) |
Protein name |
diguanylate cyclase |
Names |
EC 2.7.7.65 |
Species |
Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6) (Pseudomonas syringae pv. phaseolicola (strain 1448A / Race 6)) |
KEGG Pathway |
psp:PSPPH_3875 |
EC number |
2.7.7.-: Nucleotidyltransferases |
Protein Class |
REGULATORY COMPONENTS OF SENSORY TRANSDUCTION SYSTEM (PTHR45138) |
Descriptions
Response regulator diguanylate cyclase (WspR) is a protein that plays a key role in receiving signals from a membrane-spanning methyl-accepting chemotaxis protein, ultimately controlling biofilm formation, cytotoxicity and other adaptive phenotypes. Autoinhibition of WspR is mediated by c-di-GMP binding to an inhibitory site at the GGDEF domain, restricting the mobility of rigid GGDEF domains. This results in distinct oligomeric states that contribute to both activation and autoinhibition. The helical stalk motif plays a crucial role in facilitating tetramerization necessary for activation, and its interactions with c-di-GMP stabilize the inactive dimer state.
Autoinhibitory domains (AIDs)
Target domain |
249-253 (Inhibitory site on GGDEF domain) |
Relief mechanism |
PTM |
Assay |
Structural analysis, Split protein assay, Mutagenesis experiment |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q48F24
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q48F24-F1 | Predicted | AlphaFoldDB |
No variants for Q48F24
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| No variants for Q48F24 | |||||
No associated diseases with Q48F24
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.7.- | Nucleotidyltransferases |
| Subcellular Localization |
|
|
| PANTHER Family | PTHR45138 | REGULATORY COMPONENTS OF SENSORY TRANSDUCTION SYSTEM |
| PANTHER Subfamily | PTHR45138:SF9 | DIGUANYLATE CYCLASE DGCM-RELATED |
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
No GO annotations of cellular component
| Name | Definition |
|---|---|
| No GO annotations for cellular component |
No GO annotations of molecular function
| Name | Definition |
|---|---|
| No GO annotations for molecular function |
1 GO annotations of biological process
| Name | Definition |
|---|---|
| phosphorelay signal transduction system | A conserved series of molecular signals found in prokaryotes and eukaryotes; involves autophosphorylation of a histidine kinase and the transfer of the phosphate group to an aspartate that then acts as a phospho-donor to response regulator proteins. |
No homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| No homologous proteins | ||||
| 10 | 20 | 30 | 40 | 50 | 60 |
| MHDLQTDEIK | TPDENTAMVL | LVDDQAMIGE | AVRRGLAGHE | SIDFHFCADP | HQAIAQAVQI |
| 70 | 80 | 90 | 100 | 110 | 120 |
| KPTVILQDLV | MPGLDGLTLV | REYRSNPLTR | DIPIIVLSTK | EDPLIKSAAF | SAGANDYLVK |
| 130 | 140 | 150 | 160 | 170 | 180 |
| LPDNIELVAR | IRYHSRSYMT | LLQRDEAYRA | LRVSQQQLLD | TNLVLQRLMN | SDGLTGLSNR |
| 190 | 200 | 210 | 220 | 230 | 240 |
| RHFDEYLELE | WRRAIRDQAQ | LSLLMIDVDY | FKAYNDNFGH | LEGDEALRQV | AKAIRNSCSR |
| 250 | 260 | 270 | 280 | 290 | 300 |
| PSDLPARYGG | EEFAMVLPNT | SPGGARLLAE | KLRQSVSGMN | IPHIAPTPGS | SLTVSIGVAT |
| 310 | 320 | 330 | |||
| VTPQVGMHSR | QLILDADKGL | YLAKNNGRNQ | VAAG |