Q47152

EV
Gene name
rayT (TnpAREP, yafM, b0228, JW0218)
Protein name
REP-associated tyrosine transposase
Names
Species
Escherichia coli (strain K12)
KEGG Pathway
ecj:JW0218, eco:b0228,
EC number
Protein Class
REP-ASSOCIATED TYROSINE TRANSPOSASE (PTHR36966)

Descriptions

TnpAREP is a transposase. TnpAREP binds repetitive extragenic palindromic sequences (REP) and cleaves bacterial interspersed mosaic elements (BIMEs) both upstream and downstream of the REP sequence. 
TnpAREP is in an autoinhibited state in the crystal, as it appears to be physically blocking access of a ssDNA cleavage substrate to the active site.
This autoinhibition is likely evolved to prevent REPs from sweeping through genomes.

Autoinhibitory domains (AIDs)

130-165 (C-terminal extension) EV

Target domain

9-119 (Transposase domain)

Relief mechanism

Ligand binding

Assay

Structural analysis

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

2 structures for Q47152

Entry ID Method Resolution Chain Position Source
4ER8 X-ray 260 A A 1-165 PDB
AF-Q47152-F1 Predicted AlphaFoldDB

No variants for Q47152

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q47152

No associated diseases with Q47152

No regional properties for Q47152

Type Name Position InterPro Accession
No domain, repeats, and functional sites for Q47152

Functions

Description
EC Number
Subcellular Localization
PANTHER Family PTHR36966 REP-ASSOCIATED TYROSINE TRANSPOSASE
PANTHER Subfamily PTHR36966:SF1 REP-ASSOCIATED TYROSINE TRANSPOSASE
PANTHER Protein Class viral or transposable element protein
PANTHER Pathway Category No pathway information available

No GO annotations of cellular component

Name Definition
No GO annotations for cellular component

7 GO annotations of molecular function

Name Definition
bubble DNA binding Binding to DNA segment that contains a bubble. A bubble occurs when DNA contains a region of unpaired, single-stranded DNA flanked on both sides by regions of paired, double-stranded DNA.
DNA binding Any molecular function by which a gene product interacts selectively and non-covalently with DNA (deoxyribonucleic acid).
DNA hairpin binding Binding to a DNA region containing a hairpin. A hairpin structure forms when a DNA strand folds back on itself and intrachain base pairing occurs between inverted repeat sequences.
sequence-specific DNA binding Binding to DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding.
single-stranded DNA binding Binding to single-stranded DNA.
single-stranded DNA endodeoxyribonuclease activity Catalysis of the hydrolysis of ester linkages within a single-stranded deoxyribonucleic acid molecule by creating internal breaks.
transposase activity Catalysis of the transposition of transposable elements or transposons. Transposases are involved in recombination required for transposition and are site-specific for the transposon/transposable element.

2 GO annotations of biological process

Name Definition
DNA recombination Any process in which a new genotype is formed by reassortment of genes resulting in gene combinations different from those that were present in the parents. In eukaryotes genetic recombination can occur by chromosome assortment, intrachromosomal recombination, or nonreciprocal interchromosomal recombination. Interchromosomal recombination occurs by crossing over. In bacteria it may occur by genetic transformation, conjugation, transduction, or F-duction.
DNA transposition A type of transposition in which a transposable element (transposon) is moved to another part of a genome, either by a cut-and-paste mechanism or a replicative mechanism.

No homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
No homologous proteins
10 20 30 40 50 60
MSEYRRYYIK GGTWFFTVNL RNRRSQLLTT QYQMLRHAII KVKRDRPFEI NAWVVLPEHM
70 80 90 100 110 120
HCIWTLPEGD DDFSSRWREI KKQFTHACGL KNIWQPRFWE HAIRNTKDYR HHVDYIYINP
130 140 150 160
VKHGWVKQVS DWPFSTFHRD VARGLYPIDW AGDVTDFSAG ERIIS