Q43128

SS
Gene name
AHA10 (At1g17260, F20D23.4)
Protein name
ATPase 10, plasma membrane-type
Names
EC 7.1.2.1 , Proton pump 10
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT1G17260
EC number
7.1.2.1: Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Protein Class

Descriptions

The plasma membrane H+-ATPase belongs to the superfamily of P-type ATPases. The H+-ATPase is responsible for the ATP-fuelled ejection of protons from the cell and is an important component in the generation of the trans-membrane H+ gradient in plant cells. The H+-ATPase AHA2 is controlled by an autoinhibitory C-terminal regulatory domain and activated by 14-3-3 proteins which bind to the part of the enzyme. In two regions of the C-terminus, alteration of the residues leads to increased pump activity. One region stretches from K863 to L885 and includes two residues (Q879 and R880) that are conserved between plant and fungal H+-ATPases. The other region, incorporating S904 to L919, is situated in an extension of the C-terminus unique to plant H+-ATPases.

Autoinhibitory domains (AIDs)

870-892 (Autoinhibitory domain I) SS

Target domain

317-545 (ATPase domain)

Relief mechanism

Partner binding

Assay

902-917 (Autoinhibitory domain II) SS

Target domain

317-545 (ATPase domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q43128

Entry ID Method Resolution Chain Position Source
AF-Q43128-F1 Predicted AlphaFoldDB

61 variants for Q43128

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH00028634 7 K>N No 1000Genomes
tmp_1_5904079_C_T 8 P>S No 1000Genomes
tmp_1_5904097_A_C 14 T>P No 1000Genomes
ENSVATH04589772 28 E>D No 1000Genomes
ENSVATH01062743 31 F>I No 1000Genomes
ENSVATH11385315 35 R>T No 1000Genomes
ENSVATH11385316 46 A>T No 1000Genomes
ENSVATH01062746 47 E>D No 1000Genomes
tmp_1_5904298_A_G 52 I>V No 1000Genomes
ENSVATH01062748 62 Q>R No 1000Genomes
ENSVATH11385318 64 N>K No 1000Genomes
ENSVATH01062749 65 R>K No 1000Genomes
ENSVATH01062750 67 V>L No 1000Genomes
tmp_1_5904479_C_T 83 A>V No 1000Genomes
ENSVATH01062760 102 E>Q No 1000Genomes
ENSVATH01062761 105 T>A No 1000Genomes
ENSVATH01062762 105 T>I No 1000Genomes
tmp_1_5904880_T_G 155 I>S No 1000Genomes
ENSVATH04589783 157 V>I No 1000Genomes
tmp_1_5904937_C_G 174 A>G No 1000Genomes
tmp_1_5905089_C_A 195 P>T No 1000Genomes
ENSVATH00028649 224 S>A No 1000Genomes
ENSVATH01062791 264 V>I No 1000Genomes
tmp_1_5905517_C_A 305 L>I No 1000Genomes
ENSVATH11385555 336 C>S No 1000Genomes
ENSVATH00028654 351 K>R No 1000Genomes
ENSVATH00028656 358 V>I No 1000Genomes
tmp_1_5906121_A_T 403 I>F No 1000Genomes
ENSVATH00028664 444 K>R No 1000Genomes
ENSVATH13911502 501 S>R No 1000Genomes
ENSVATH04589796 502 G>D No 1000Genomes
ENSVATH04589797 535 M>V No 1000Genomes
tmp_1_5906885_A_T 540 Y>F No 1000Genomes
ENSVATH04589798 546 L>I No 1000Genomes
tmp_1_5907115_A_G 587 K>R No 1000Genomes
tmp_1_5907192_G_A 613 A>T No 1000Genomes
ENSVATH11385562 635 I>V No 1000Genomes
ENSVATH04589812 668 T>I No 1000Genomes
ENSVATH04589813 686 I>M No 1000Genomes
ENSVATH13911503 704 P>S No 1000Genomes
ENSVATH00028682 729 L>S No 1000Genomes
tmp_1_5907918_T_G 743 F>V No 1000Genomes
ENSVATH11386071 756 N>K No 1000Genomes
ENSVATH01062870 759 Q>K No 1000Genomes
ENSVATH01062872 764 M>V No 1000Genomes
ENSVATH11386072 792 T>I No 1000Genomes
tmp_1_5908305_A_G 813 N>S No 1000Genomes
ENSVATH01062884 837 F>S No 1000Genomes
ENSVATH04589823 843 V>F No 1000Genomes
ENSVATH01062885 844 I>L No 1000Genomes
ENSVATH11386073 858 N>H No 1000Genomes
ENSVATH00028691 858 N>S No 1000Genomes
ENSVATH00028692 862 D>A No 1000Genomes
ENSVATH01062889 879 S>T No 1000Genomes
ENSVATH01062890 880 P>A No 1000Genomes
ENSVATH00028695 889 R>H No 1000Genomes
tmp_1_5908642_A_T 892 E>V No 1000Genomes
ENSVATH04589824 895 R>I No 1000Genomes
ENSVATH00028696 895 R>S No 1000Genomes
ENSVATH01062891 896 G>E No 1000Genomes
ENSVATH00028699 938 Q>P No 1000Genomes

No associated diseases with Q43128

3 regional properties for Q43128

Type Name Position InterPro Accession
domain Cation-transporting P-type ATPase, N-terminal 21 - 93 IPR004014
ptm P-type ATPase, phosphorylation site 337 - 343 IPR018303
domain P-type ATPase, haloacid dehalogenase domain 317 - 645 IPR044492

Functions

Description
EC Number 7.1.2.1 Hydron translocation linked to the hydrolysis of a nucleoside triphosphate
Subcellular Localization
  • Membrane; Multi-pass membrane protein
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
plant-type vacuole membrane The lipid bilayer surrounding a vacuole that retains the same shape regardless of cell cycle phase. The membrane separates its contents from the cytoplasm of the cell. An example of this component is found in Arabidopsis thaliana.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
plasmodesma A fine cytoplasmic channel, found in all higher plants, that connects the cytoplasm of one cell to that of an adjacent cell.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
ATPase-coupled monoatomic cation transmembrane transporter activity Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction
metal ion binding Binding to a metal ion.
P-type ion transporter activity Enables the transfer of a solute or solutes from one side of a membrane to the other according to the reaction
P-type proton-exporting transporter activity Enables the transfer of protons from one side of a membrane to the other according to the reaction

5 GO annotations of biological process

Name Definition
proanthocyanidin biosynthetic process The chemical reactions and pathways resulting in the formation of proanthocyanidin.
proton export across plasma membrane The directed movement of hydrogen ions (protons) from inside a cell, across the plasma membrane and into the extracellular region.
seed coat development The process whose specific outcome is the progression of the seed coat over time, from its formation to the mature structure.
vacuolar acidification Any process that reduces the pH of the vacuole, measured by the concentration of the hydrogen ion.
vacuole organization A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a vacuole.

15 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P83970 ha1 Plasma membrane ATPase Triticum aestivum (Wheat) SS
Q80XR2 Atp2c1 Calcium-transporting ATPase type 2C member 1 Mus musculus (Mouse) PR
A7L9Z8 Atp2c2 Calcium-transporting ATPase type 2C member 2 Mus musculus (Mouse) PR
Q7XPY2 Os04g0656100 Plasma membrane ATPase Oryza sativa subsp. japonica (Rice) SS
P20431 AHA3 ATPase 3, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P20649 AHA1 ATPase 1, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV11 AHA11 ATPase 11, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9LY32 AHA7 ATPase 7, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2A0 AHA8 ATPase 8, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SH76 AHA6 ATPase 6, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJB3 AHA5 ATPase 5, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
Q9SU58 AHA4 ATPase 4, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P19456 AHA2 ATPase 2, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) EV
Q42556 AHA9 ATPase 9, plasma membrane-type Arabidopsis thaliana (Mouse-ear cress) SS
P22180 LHA1 Plasma membrane ATPase 1 Solanum lycopersicum (Tomato) (Lycopersicon esculentum) SS
10 20 30 40 50 60
MAEDLDKPLL DPDTFNRKGI DLGILPLEEV FEYLRTSPQG LLSGDAEERL KIFGPNRLEE
70 80 90 100 110 120
KQENRFVKFL GFMWNPLSWV MEAAALMAIA LANSQSLGPD WEDFTGIVCL LLINATISFF
130 140 150 160 170 180
EENNAGNAAA ALMARLALKT RVLRDGQWQE QDASILVPGD IISIKLGDII PADARLLEGD
190 200 210 220 230 240
PLKIDQSVLT GESLPVTKKK GEQVFSGSTC KQGEIEAVVI ATGSTTFFGK TARLVDSTDV
250 260 270 280 290 300
TGHFQQVLTS IGNFCICSIA VGMVLEIIIM FPVQHRSYRI GINNLLVLLI GGIPIAMPTV
310 320 330 340 350 360
LSVTLAIGSH RLSQQGAITK RMTAIEEMAG MDVLCCDKTG TLTLNSLTVD KNLIEVFVDY
370 380 390 400 410 420
MDKDTILLLA GRASRLENQD AIDAAIVSML ADPREARANI REIHFLPFNP VDKRTAITYI
430 440 450 460 470 480
DSDGKWYRAT KGAPEQVLNL CQQKNEIAQR VYAIIDRFAE KGLRSLAVAY QEIPEKSNNS
490 500 510 520 530 540
PGGPWRFCGL LPLFDPPRHD SGETILRALS LGVCVKMITG DQLAIAKETG RRLGMGTNMY
550 560 570 580 590 600
PSSSLLGHNN DEHEAIPVDE LIEMADGFAG VFPEHKYEIV KILQEMKHVV GMTGDGVNDA
610 620 630 640 650 660
PALKKADIGI AVADATDAAR SSADIVLTDP GLSVIISAVL TSRAIFQRMR NYTVYAVSIT
670 680 690 700 710 720
IRIVLGFTLL ALIWEYDFPP FMVLIIAILN DGTIMTISKD RVRPSPTPES WKLNQIFATG
730 740 750 760 770 780
IVIGTYLALV TVLFYWIIVS TTFFEKHFHV KSIANNSEQV SSAMYLQVSI ISQALIFVTR
790 800 810 820 830 840
SRGWSFFERP GTLLIFAFIL AQLAATLIAV YANISFAKIT GIGWRWAGVI WLYSLIFYIP
850 860 870 880 890 900
LDVIKFVFHY ALSGEAWNLV LDRKTAFTYK KDYGKDDGSP NVTISQRSRS AEELRGSRSR
910 920 930 940
ASWIAEQTRR RAEIARLLEV HSVSRHLESV IKLKQIDQRM IRAAHTV