Q3UIZ8
SS
Gene name |
Mylk3 |
Protein name |
Myosin light chain kinase 3 |
Names |
EC 2.7.11.18 , Cardiac MyBP-C-associated Ca/CaM kinase , Cardiac MLCK |
Species |
Mus musculus (Mouse) |
KEGG Pathway |
mmu:213435 |
EC number |
2.7.11.18: Protein-serine/threonine kinases |
Protein Class |
|
Descriptions
Myosin light chain kinases (MLCK) are members of the family of Ca2+-calmodulin-dependent protein kinases. This enzyme contains an autoinhibitory pseudosubstrate sequence that blocks the substrate-binding site of the kinase. The autoinhibitory sequence makes an extensive network of contacts on the surface of the larger C-domain of the catalytic core extending towards the catalytic cleft with the CaM-binding sequence to block the regulatory light chain of sarcomeric myosin, but not ATP binding in the catalytic core N-domain.
Autoinhibitory domains (AIDs)
Target domain |
491-746 (Protein kinase domain) |
Relief mechanism |
Partner binding |
Assay |
|
Accessory elements
631-653 (Activation loop from InterPro)
Target domain |
491-746 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
631-653 (Activation loop from InterPro)
Target domain |
491-746 (Protein kinase domain) |
Relief mechanism |
|
Assay |
|
References
Autoinhibited structure
Activated structure
1 structures for Q3UIZ8
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q3UIZ8-F1 | Predicted | AlphaFoldDB |
56 variants for Q3UIZ8
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs251154222 | 14 | P>H | No | EVA | |
| rs3388998251 | 112 | A>V | No | EVA | |
| rs3388998472 | 150 | P>S | No | EVA | |
| rs36584349 | 153 | K>I | No | EVA | |
| rs33360271 | 158 | D>H | No | EVA | |
| rs36323526 | 175 | N>K | No | EVA | |
| rs38204699 | 187 | A>P | No | EVA | |
| rs37115963 | 192 | W>R | No | EVA | |
| rs3388990580 | 194 | E>G | No | EVA | |
| rs3388998422 | 239 | Q>* | No | EVA | |
| rs47034756 | 243 | P>R | No | EVA | |
| rs251440228 | 249 | R>G | No | EVA | |
| rs3389002220 | 254 | A>V | No | EVA | |
| rs3388973933 | 267 | A>S | No | EVA | |
| rs3388994883 | 271 | I>F | No | EVA | |
| rs37458824 | 284 | G>A | No | EVA | |
| rs38919758 | 287 | E>G | No | EVA | |
| rs48234972 | 292 | K>M | No | EVA | |
| rs3388998316 | 313 | L>P | No | EVA | |
| rs251317334 | 314 | G>E | No | EVA | |
| rs36610272 | 322 | I>T | No | EVA | |
| rs3399402960 | 325 | G>S | No | EVA | |
| rs3388973940 | 348 | Q>K | No | EVA | |
| rs3388991430 | 349 | S>I | No | EVA | |
| rs3388998019 | 352 | P>L | No | EVA | |
| rs37429337 | 352 | P>S | No | EVA | |
| rs3399364776 | 353 | I>N | No | EVA | |
| rs3388991497 | 356 | A>V | No | EVA | |
| rs3399351814 | 358 | A>T | No | EVA | |
| rs264616968 | 363 | T>I | No | EVA | |
| rs3388990614 | 389 | M>K | No | EVA | |
| rs37310997 | 391 | A>V | No | EVA | |
| rs3388991451 | 395 | I>L | No | EVA | |
| rs51858128 | 397 | S>P | No | EVA | |
| rs256143501 | 440 | A>T | No | EVA | |
| rs36314041 | 452 | L>V | No | EVA | |
| rs3388994884 | 482 | D>H | No | EVA | |
| rs3388994873 | 522 | I>V | No | EVA | |
| rs3388985252 | 540 | M>T | No | EVA | |
| rs37982779 | 544 | S>C | No | EVA | |
| rs3388994692 | 556 | E>D | No | EVA | |
| rs30530050 | 559 | S>N | No | EVA | |
| rs3388994694 | 595 | Q>* | No | EVA | |
| rs3388990628 | 659 | V>I | No | EVA | |
| rs3388973960 | 674 | V>L | No | EVA | |
| rs33212606 | 678 | T>I | No | EVA | |
| rs3389002209 | 688 | F>V | No | EVA | |
| rs3388979475 | 692 | T>R | No | EVA | |
| rs3388949198 | 698 | N>I | No | EVA | |
| rs32723361 | 747 | S>N | No | EVA | |
| rs3388995892 | 749 | L>M | No | EVA | |
| rs3399376307 | 761 | R>G | No | EVA | |
| rs3388967522 | 764 | Q>H | No | EVA | |
| rs3399429878 | 779 | F>RSLLTAVTTWKCFLK* | No | EVA | |
| rs49163301 | 783 | T>A | No | EVA | |
| rs3388990555 | 785 | V>A | No | EVA |
No associated diseases with Q3UIZ8
Functions
| Description | ||
|---|---|---|
| EC Number | 2.7.11.18 | Protein-serine/threonine kinases |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
3 GO annotations of cellular component
| Name | Definition |
|---|---|
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| cytosol | The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
3 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| calmodulin-dependent protein kinase activity | Calmodulin-dependent catalysis of the reactions |
| myosin light chain kinase activity | Catalysis of the reaction |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| cardiac myofibril assembly | The process whose specific outcome is the progression of the cardiac myofibril over time, from its formation to the mature structure. A cardiac myofibril is a myofibril specific to cardiac muscle cells. |
| cellular response to interleukin-1 | Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an interleukin-1 stimulus. |
| intracellular signal transduction | The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell. |
| positive regulation of apoptotic process | Any process that activates or increases the frequency, rate or extent of cell death by apoptotic process. |
| positive regulation of sarcomere organization | Any process that increases the rate, frequency or extent of myofibril assembly by organization of muscle actomyosin into sarcomeres. The sarcomere is the repeating unit of a myofibril in a muscle cell, composed of an array of overlapping thick and thin filaments between two adjacent Z discs. |
| protein phosphorylation | The process of introducing a phosphate group on to a protein. |
| regulation of vascular permeability involved in acute inflammatory response | Any process that modulates the extent to which blood vessels can be pervaded by fluid contributing to an acute inflammatory response. |
| sarcomere organization | The myofibril assembly process that results in the organization of muscle actomyosin into sarcomeres. The sarcomere is the repeating unit of a myofibril in a muscle cell, composed of an array of overlapping thick and thin filaments between two adjacent Z discs. |
| sarcomerogenesis | The process in which sarcomeres are added in series within a fiber. |
105 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| A4IFM7 | MYLK2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Bos taurus (Bovine) | SS |
| Q0V7M1 | PSKH1 | Serine/threonine-protein kinase H1 | Bos taurus (Bovine) | SS |
| Q0VD22 | STK33 | Serine/threonine-protein kinase 33 | Bos taurus (Bovine) | PR |
| Q96NX5 | CAMK1G | Calcium/calmodulin-dependent protein kinase type 1G | Homo sapiens (Human) | SS |
| O15075 | DCLK1 | Serine/threonine-protein kinase DCLK1 | Homo sapiens (Human) | EV |
| Q14012 | CAMK1 | Calcium/calmodulin-dependent protein kinase type 1 | Homo sapiens (Human) | EV |
| Q9UIK4 | DAPK2 | Death-associated protein kinase 2 | Homo sapiens (Human) | EV |
| O94768 | STK17B | Serine/threonine-protein kinase 17B | Homo sapiens (Human) | PR |
| Q86YV6 | MYLK4 | Myosin light chain kinase family member 4 | Homo sapiens (Human) | SS |
| Q16566 | CAMK4 | Calcium/calmodulin-dependent protein kinase type IV | Homo sapiens (Human) | SS |
| P11801 | PSKH1 | Serine/threonine-protein kinase H1 | Homo sapiens (Human) | SS |
| Q8N568 | DCLK2 | Serine/threonine-protein kinase DCLK2 | Homo sapiens (Human) | PR |
| Q9UEE5 | STK17A | Serine/threonine-protein kinase 17A | Homo sapiens (Human) | SS |
| O75962 | TRIO | Triple functional domain protein | Homo sapiens (Human) | EV |
| Q9H1R3 | MYLK2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Homo sapiens (Human) | EV |
| Q8WWF8 | CAPSL | Calcyphosin-like protein | Homo sapiens (Human) | PR |
| P53355 | DAPK1 | Death-associated protein kinase 1 | Homo sapiens (Human) | EV |
| Q6P2M8 | PNCK | Calcium/calmodulin-dependent protein kinase type 1B | Homo sapiens (Human) | SS |
| Q9BYT3 | STK33 | Serine/threonine-protein kinase 33 | Homo sapiens (Human) | PR |
| Q8NCB2 | CAMKV | CaM kinase-like vesicle-associated protein | Homo sapiens (Human) | SS |
| O43293 | DAPK3 | Death-associated protein kinase 3 | Homo sapiens (Human) | PR |
| Q8IU85 | CAMK1D | Calcium/calmodulin-dependent protein kinase type 1D | Homo sapiens (Human) | SS |
| Q32MK0 | MYLK3 | Myosin light chain kinase 3 | Homo sapiens (Human) | SS |
| Q8BG48 | Stk17b | Serine/threonine-protein kinase 17B | Mus musculus (Mouse) | PR |
| Q6PGN3 | Dclk2 | Serine/threonine-protein kinase DCLK2 | Mus musculus (Mouse) | PR |
| Q8VDF3 | Dapk2 | Death-associated protein kinase 2 | Mus musculus (Mouse) | EV |
| Q6P8Y1 | Capsl | Calcyphosin-like protein | Mus musculus (Mouse) | PR |
| P08414 | Camk4 | Calcium/calmodulin-dependent protein kinase type IV | Mus musculus (Mouse) | SS |
| Q9JLM8 | Dclk1 | Serine/threonine-protein kinase DCLK1 | Mus musculus (Mouse) | SS |
| Q80YE7 | Dapk1 | Death-associated protein kinase 1 | Mus musculus (Mouse) | SS |
| Q62407 | Speg | Striated muscle-specific serine/threonine-protein kinase | Mus musculus (Mouse) | SS |
| Q0KL02 | Trio | Triple functional domain protein | Mus musculus (Mouse) | SS |
| Q924X7 | Stk33 | Serine/threonine-protein kinase 33 | Mus musculus (Mouse) | PR |
| O54784 | Dapk3 | Death-associated protein kinase 3 | Mus musculus (Mouse) | PR |
| P07934 | Phkg1 | Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform | Mus musculus (Mouse) | SS |
| Q9DB30 | Phkg2 | Phosphorylase b kinase gamma catalytic chain, liver/testis isoform | Mus musculus (Mouse) | PR |
| P28652 | Camk2b | Calcium/calmodulin-dependent protein kinase type II subunit beta | Mus musculus (Mouse) | SS |
| P11798 | Camk2a | Calcium/calmodulin-dependent protein kinase type II subunit alpha | Mus musculus (Mouse) | PR |
| Q923T9 | Camk2g | Calcium/calmodulin-dependent protein kinase type II subunit gamma | Mus musculus (Mouse) | SS |
| Q6PHZ2 | Camk2d | Calcium/calmodulin-dependent protein kinase type II subunit delta | Mus musculus (Mouse) | SS |
| Q8VCR8 | Mylk2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Mus musculus (Mouse) | SS |
| Q91YA2 | Pskh1 | Serine/threonine-protein kinase H1 | Mus musculus (Mouse) | SS |
| Q9QYK9 | Pnck | Calcium/calmodulin-dependent protein kinase type 1B | Mus musculus (Mouse) | PR |
| Q91VB2 | Camk1g | Calcium/calmodulin-dependent protein kinase type 1G | Mus musculus (Mouse) | SS |
| Q8BW96 | Camk1d | Calcium/calmodulin-dependent protein kinase type 1D | Mus musculus (Mouse) | SS |
| Q3UHL1 | Camkv | CaM kinase-like vesicle-associated protein | Mus musculus (Mouse) | SS |
| Q91YS8 | Camk1 | Calcium/calmodulin-dependent protein kinase type 1 | Mus musculus (Mouse) | SS |
| Q7TNJ7 | Camk1g | Calcium/calmodulin-dependent protein kinase type 1G | Rattus norvegicus (Rat) | SS |
| P20689 | Mylk2 | Myosin light chain kinase 2, skeletal/cardiac muscle | Rattus norvegicus (Rat) | SS |
| F1M0Z1 | Trio | Triple functional domain protein | Rattus norvegicus (Rat) | SS |
| Q91XS8 | Stk17b | Serine/threonine-protein kinase 17B | Rattus norvegicus (Rat) | PR |
| O08875 | Dclk1 | Serine/threonine-protein kinase DCLK1 | Rattus norvegicus (Rat) | PR |
| O70150 | Pnck | Calcium/calmodulin-dependent protein kinase type 1B | Rattus norvegicus (Rat) | PR |
| Q63092 | Camkv | CaM kinase-like vesicle-associated protein | Rattus norvegicus (Rat) | SS |
| Q63638 | Speg | Striated muscle-specific serine/threonine-protein kinase | Rattus norvegicus (Rat) | SS |
| O88764 | Dapk3 | Death-associated protein kinase 3 | Rattus norvegicus (Rat) | PR |
| P13234 | Camk4 | Calcium/calmodulin-dependent protein kinase type IV | Rattus norvegicus (Rat) | SS |
| Q63450 | Camk1 | Calcium/calmodulin-dependent protein kinase type 1 | Rattus norvegicus (Rat) | EV |
| P97924 | Kalrn | Kalirin | Rattus norvegicus (Rat) | SS |
| Q5MPA9 | Dclk2 | Serine/threonine-protein kinase DCLK2 | Rattus norvegicus (Rat) | PR |
| E9PT87 | Mylk3 | Myosin light chain kinase 3 | Rattus norvegicus (Rat) | SS |
| Q5VQQ5 | CPK2 | Calcium-dependent protein kinase 2 | Oryza sativa subsp. japonica (Rice) | SS |
| Q8LPZ7 | CPK3 | Calcium-dependent protein kinase 3 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6K968 | CPK6 | Calcium-dependent protein kinase 6 | Oryza sativa subsp. japonica (Rice) | SS |
| P53684 | CPK7 | Calcium-dependent protein kinase 7 | Oryza sativa subsp japonica (Rice) | PR |
| Q75GE8 | CPK8 | Calcium-dependent protein kinase 8 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6I5I8 | CPK16 | Calcium-dependent protein kinase 16 | Oryza sativa subsp. japonica (Rice) | SS |
| Q6AVM3 | CCAMK | Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase | Oryza sativa subsp japonica (Rice) | PR |
| Q84SL0 | CPK20 | Calcium-dependent protein kinase 20 | Oryza sativa subsp. japonica (Rice) | SS |
| P53682 | CPK23 | Calcium-dependent protein kinase 23 | Oryza sativa subsp japonica (Rice) | PR |
| Q9TXJ0 | cmk-1 | Calcium/calmodulin-dependent protein kinase type 1 | Caenorhabditis elegans | PR |
| O44997 | dapk-1 | Death-associated protein kinase dapk-1 | Caenorhabditis elegans | SS |
| Q95QC4 | zyg-8 | Serine/threonine-protein kinase zyg-8 | Caenorhabditis elegans | PR |
| P28583 | Calcium-dependent protein kinase SK5 | Glycine max (Soybean) (Glycine hispida) | PR | |
| Q9M9V8 | CPK10 | Calcium-dependent protein kinase 10 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q39016 | CPK11 | Calcium-dependent protein kinase 11 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FX86 | CRK8 | CDPK-related kinase 8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SSF8 | CPK30 | Calcium-dependent protein kinase 30 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38872 | CPK6 | Calcium-dependent protein kinase 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q7XJR9 | CPK16 | Calcium-dependent protein kinase 16 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SIQ7 | CPK24 | Calcium-dependent protein kinase 24 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SJ61 | CPK25 | Calcium-dependent protein kinase 25 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9ZV15 | CPK20 | Calcium-dependent protein kinase 20 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| P93759 | GK-1 | Calcium-dependent protein kinase 14 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8S8S2 | LPEAT2 | Lysophospholipid acyltransferase LPEAT2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38870 | CPK2 | Calcium-dependent protein kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9LJL9 | CRK2 | CDPK-related kinase 2 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SG12 | CRK6 | CDPK-related kinase 6 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9SCS2 | CRK5 | CDPK-related kinase 5 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q8W4I7 | CPK13 | Calcium-dependent protein kinase 13 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q6NLQ6 | CPK32 | Calcium-dependent protein kinase 32 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9ZSA4 | CPK27 | Calcium-dependent protein kinase 27 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38869 | CPK4 | Calcium-dependent protein kinase 4 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q42479 | CPK3 | Calcium-dependent protein kinase 3 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q1PE17 | CPK18 | Calcium-dependent protein kinase 18 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q06850 | CPK1 | Calcium-dependent protein kinase 1 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q9FMP5 | CPK17 | Calcium-dependent protein kinase 17 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q38873 | CPK7 | Calcium-dependent protein kinase 7 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q3E9C0 | CPK34 | Calcium-dependent protein kinase 34 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q42438 | CPK8 | Calcium-dependent protein kinase 8 | Arabidopsis thaliana (Mouse-ear cress) | PR |
| Q42396 | CPK12 | Calcium-dependent protein kinase 12 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q9FKW4 | CPK28 | Calcium-dependent protein kinase 28 | Arabidopsis thaliana (Mouse-ear cress) | SS |
| Q1LUA6 | trio | Triple functional domain protein | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| Q7SY49 | camkv | CaM kinase-like vesicle-associated protein | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| A8C984 | mylk3 | Myosin light chain kinase 3 | Danio rerio (Zebrafish) (Brachydanio rerio) | SS |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MSGVSEEDPE | GLAPQGLPAL | GGACLATMDK | KLNVLTEKVD | RLLHFQEDVT | EKLQCVCQGM |
| 70 | 80 | 90 | 100 | 110 | 120 |
| DHLEQDLHRL | EASRELSLAG | SGSTPPTTAQ | AAWPEVLELV | RAVRQEGAQH | GARLEALFKM |
| 130 | 140 | 150 | 160 | 170 | 180 |
| VVAVDRAITL | VGSTFQNSKV | ADFIMQGTVP | GRKGSLADGP | EENKEQAEVA | GVKPNHVLTT |
| 190 | 200 | 210 | 220 | 230 | 240 |
| GGVQADASRT | LWEESQKEDI | PVRTVEGLPL | IINTSLKGAD | LTQAGASLRQ | GVEVLGPGQV |
| 250 | 260 | 270 | 280 | 290 | 300 |
| PLPTEAESRL | PETASENTGA | TLELSVAIDR | ISEVLTSLKM | SQGGGQETSS | SKPDCWLSEE |
| 310 | 320 | 330 | 340 | 350 | 360 |
| AMRLSSGPLP | QPLGPLTPDS | DIHSGDALPR | IPINMQEMAT | PGELLETQSG | SPIGSAEAPG |
| 370 | 380 | 390 | 400 | 410 | 420 |
| LGTVLEDQIP | KGARPFPPLP | KRSSNNGGMS | AEEEIGSGAE | PMRGPSLATR | DWRDETVGTT |
| 430 | 440 | 450 | 460 | 470 | 480 |
| DLQQGIDPGA | VSPEPGKDHA | AQGPGRTEAG | RLSSAAEAAI | VVLDDSAAPP | APFEHRVVSI |
| 490 | 500 | 510 | 520 | 530 | 540 |
| KDTLISAGYT | VSQHEVLGGG | RFGQVHRCTE | RSTGLALAAK | IIKVKNVKDR | EDVKNEVNIM |
| 550 | 560 | 570 | 580 | 590 | 600 |
| NQLSHVNLIQ | LYDAFESKSS | FTLIMEYVDG | GELFDRITDE | KYHLTELDVV | LFTRQICEGV |
| 610 | 620 | 630 | 640 | 650 | 660 |
| HYLHQHYILH | LDLKPENILC | VSQTGHQIKI | IDFGLARRYK | PREKLKVNFG | TPEFLAPEVV |
| 670 | 680 | 690 | 700 | 710 | 720 |
| NYEFVSFPTD | MWSVGVITYM | LLSGLSPFLG | ETDAETMNFI | VNCSWDFDAD | TFKGLSEEAK |
| 730 | 740 | 750 | 760 | 770 | 780 |
| DFVSRLLVKE | KSCRMSATQC | LKHEWLSHLP | AKASGSNVRL | RSQQLLQKYM | AQSKWKKHFH |
| 790 | |||||
| VVTAVNRLRK | FPTCP |