Q3UH66

SS
Gene name
Wnk2
Protein name
Serine/threonine-protein kinase WNK2
Names
Protein kinase lysine-deficient 2, Protein kinase with no lysine 2
Species
Mus musculus (Mouse)
KEGG Pathway
mmu:75607
EC number
2.7.11.1: Protein-serine/threonine kinases
Protein Class

Descriptions

WNK family protein kinases are large enzymes that contain the catalytic lysine in a unique position compared with all other protein kinases. Rat WNK1 (Q9JIH7) contains an autoinhibitory region C-terminal to the catalytic kinase domain.

Autoinhibitory domains (AIDs)

488-511 (Autoinhibitory region) SS

Target domain

195-453 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

341-362 (Activation loop from InterPro)

Target domain

195-453 (Protein kinase domain)

Relief mechanism

Assay

References

Autoinhibited structure

Activated structure

1 structures for Q3UH66

Entry ID Method Resolution Chain Position Source
AF-Q3UH66-F1 Predicted AlphaFoldDB

No variants for Q3UH66

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q3UH66

No associated diseases with Q3UH66

8 regional properties for Q3UH66

Type Name Position InterPro Accession
domain CARD domain 26 - 120 IPR001315-1
domain CARD domain 134 - 218 IPR001315-2
repeat Leucine-rich repeat 898 - 920 IPR001611-1
repeat Leucine-rich repeat 927 - 948 IPR001611-2
repeat Leucine-rich repeat 981 - 1005 IPR001611-3
domain NACHT nucleoside triphosphatase 293 - 462 IPR007111
domain NOD2, winged helix domain 546 - 587 IPR041075
domain NACHT, LRR and PYD domains-containing protein, helical domain HD2 603 - 757 IPR041267

Functions

Description
EC Number 2.7.11.1 Protein-serine/threonine kinases
Subcellular Localization
  • Cytoplasm
  • Cell membrane
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
cytosol The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
plasma membrane The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.

6 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
chloride channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a chloride channel.
potassium channel inhibitor activity Binds to and stops, prevents, or reduces the activity of a potassium channel.
protein serine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate.
protein serine/threonine kinase activity Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.
protein serine/threonine/tyrosine kinase activity Catalysis of the reactions: ATP + a protein serine = ADP + protein serine phosphate; ATP + a protein threonine = ADP + protein threonine phosphate; and ATP + a protein tyrosine = ADP + protein tyrosine phosphate.

10 GO annotations of biological process

Name Definition
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
ion homeostasis Any process involved in the maintenance of an internal steady state of ions within an organism or cell.
negative regulation of cell population proliferation Any process that stops, prevents or reduces the rate or extent of cell proliferation.
negative regulation of ERK1 and ERK2 cascade Any process that stops, prevents, or reduces the frequency, rate or extent of signal transduction mediated by the ERK1 and ERK2 cascade.
negative regulation of sodium ion transport Any process that decreases the frequency, rate or extent of the directed movement of sodium ions (Na+) into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore.
positive regulation of canonical Wnt signaling pathway Any process that increases the rate, frequency, or extent of the Wnt signaling pathway through beta-catenin, the series of molecular signals initiated by binding of a Wnt protein to a frizzled family receptor on the surface of the target cell, followed by propagation of the signal via beta-catenin, and ending with a change in transcription of target genes.
positive regulation of potassium ion import across plasma membrane Any process that activates or increases the frequency, rate or extent of potassium ion import across the plasma membrane.
positive regulation of sodium ion transmembrane transporter activity Any process that activates or increases the frequency, rate or extent of sodium ion transmembrane transporter activity.
protein autophosphorylation The phosphorylation by a protein of one or more of its own amino acid residues (cis-autophosphorylation), or residues on an identical protein (trans-autophosphorylation).
protein phosphorylation The process of introducing a phosphate group on to a protein.

17 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q96J92 WNK4 Serine/threonine-protein kinase WNK4 Homo sapiens (Human) SS
Q9H4A3 WNK1 Serine/threonine-protein kinase WNK1 Homo sapiens (Human) SS
Q9BYP7 WNK3 Serine/threonine-protein kinase WNK3 Homo sapiens (Human) SS
Q9Y3S1 WNK2 Serine/threonine-protein kinase WNK2 Homo sapiens (Human) SS
Q99J45 Nrbp1 Nuclear receptor-binding protein Mus musculus (Mouse) PR
Q80XP9 Wnk3 Serine/threonine-protein kinase WNK3 Mus musculus (Mouse) SS
P83741 Wnk1 Serine/threonine-protein kinase WNK1 Mus musculus (Mouse) SS
Q80UE6 Wnk4 Serine/threonine-protein kinase WNK4 Mus musculus (Mouse) SS
Q6R2V0 WNK1 Serine/threonine-protein kinase WNK1 Sus scrofa (Pig) SS
Q9JIH7 Wnk1 Serine/threonine-protein kinase WNK1 Rattus norvegicus (Rat) EV
Q7TPK6 Wnk4 Serine/threonine-protein kinase WNK4 Rattus norvegicus (Rat) SS
X5M5N0 wnk-1 Serine/threonine-protein kinase WNK Caenorhabditis elegans SS
Q8RXE5 WNK10 Probable serine/threonine-protein kinase WNK10 Arabidopsis thaliana (Mouse-ear cress) PR
Q944Q0 WNK8 Serine/threonine-protein kinase WNK8 Arabidopsis thaliana (Mouse-ear cress) PR
Q9LVL5 WNK4 Probable serine/threonine-protein kinase WNK4 Arabidopsis thaliana (Mouse-ear cress) PR
Q9STK6 WNK3 Probable serine/threonine-protein kinase WNK3 Arabidopsis thaliana (Mouse-ear cress) PR
Q8LST2 WNK7 Probable serine/threonine-protein kinase WNK7 Arabidopsis thaliana (Mouse-ear cress) PR
10 20 30 40 50 60
MDGDGGRRDA PGALMEAGRG TGSAGMAEPR ARAARLGPQR FLRRSVVESD QEEPPGLEAA
70 80 90 100 110 120
ETPSAQPPQP LQRRVLLLCK TRRLIAERAR GRPAAPAPAA PAAPPGSPSV PSDPGPERAG
130 140 150 160 170 180
TQEPSPDPTT ASAAATQVPD GGPRQEEAPA PTQEDAGTTE AKPEPGRARK DEPEEEEDDE
190 200 210 220 230 240
DDLKAVATSL DGRFLKFDIE LGRGSFKTVY KGLDTETWVE VAWCELQDRK LTKLERQRFK
250 260 270 280 290 300
EEAEMLKGLQ HPNIVRFYDF WESSAKGKRC IVLVTELMTS GTLKTYLKRF KVMKPKVLRS
310 320 330 340 350 360
WCRQILKGLL FLHTRTPPII HRDLKCDNIF ITGPTGSVKI GDLGLATLKR ASFAKSVIGT
370 380 390 400 410 420
PEFMAPEMYE EHYDESVDVY AFGMCMLEMA TSEYPYSECQ NAAQIYRKVT CGIKPASFEK
430 440 450 460 470 480
VHDPEIKEII GECICKNKEE RYEIKDLLSH AFFAEDTGVR VELAEEDHGR KSTIALRLWV
490 500 510 520 530 540
EDPKKLKGKP KDNGAIEFTF DLEKETPDEV AQEMIDSGFF HESDVKIVAK SIRDRVALIQ
550 560 570 580 590 600
WRRERIWPAL QSQEPKDSGS PDKARGLPAP LQVQVTYHAQ SGQPGQPEPE EPEADQHLLP
610 620 630 640 650 660
PTLPASVTSL ASDSTFDSGQ GSTVYSDSQS SQQSMVLSSL VDTAPTPASC VCSPPVSEGP
670 680 690 700 710 720
GLTHSLPTLG AFQQPATVPG LSVGPVPPPA RPPLLQQHFP ESSMSFTPVL PPPSTPVPTG
730 740 750 760 770 780
PSQPAPPVQQ PLPMAQPPTL PQVLAPQPMG TVQPVPSHLP PYLAPTSQVV APAQLKPLQM
790 800 810 820 830 840
PQPPLQPLAQ VPPQMPQMPV VPPITPLTGL DGLPQTLTDL PAANVAPVPP PQYFSPAVIL
850 860 870 880 890 900
PSLTTPLPTS PALPMQAVKL PHPPGTPLAV PCQTIVPNAP AAIPLLAVAP QGVAALSIHP
910 920 930 940 950 960
AVAQIPAQPV YPAAFPQMVP GDIPPSPHHT VQSLRATPPQ LASPVPPQPV QPSVIHLPEQ
970 980 990 1000 1010 1020
AAPTAASGTQ VLLGHPPSYT ADVAAPVSAV SLPPAVLSPP LPDTLLPTVP DLLPKVPSSL
1030 1040 1050 1060 1070 1080
APTVVAASQS APAQTSSLLL PTNPPLPTGP AVAGPCPAVQ LMVEVAQEEQ VSQDKPPGPP
1090 1100 1110 1120 1130 1140
QSSESFGGSD VTSGRDLSDS CEGTFGGGRL EGRTARKHHR RSTRARSRQE RASRPRLTIL
1150 1160 1170 1180 1190 1200
NVCNTGDKMV ECQLETHNHK MVTFKFDLDG DAPDEIATYM VEHDFILPAE RETFIEQMKD
1210 1220 1230 1240 1250 1260
VMDKAEDMLS EDTDADHGSD TGTSPPHLGT CGLATGEENR QSQANAPVYQ QNVLHTGKRW
1270 1280 1290 1300 1310 1320
FIICPVAEHP ATDTSESSPP LPLSSLQPEA SQDPAPYPDQ LSLTDKPSFP AAQQLLSQAG
1330 1340 1350 1360 1370 1380
SSNPPGGASA PLAPSSPPVT TVIPAAPATS TVPESAAGTA MQAGGPGTHQ GPASVHETLQ
1390 1400 1410 1420 1430 1440
PLAETRSAQC TAQPLSTGQG PCTPALEASR CSTGLGEPIS TREVSTQGEP LPASVPEPSP
1450 1460 1470 1480 1490 1500
PTGATQSVPG QPPPPLPITV GAISLAAPQL PSPPLGPTAP PPPPSALESD GEGPPPRVGF
1510 1520 1530 1540 1550 1560
VDNTIKSLDE KLRTLLYQEH VPTSSASAGT PMEASDRDFT LEPLRGDLPS ALSDKTPSLT
1570 1580 1590 1600 1610 1620
QQTQPSLEKS ETAPAGWALA QREQGASSPM TAESSSSNTL GCDSDAGQVA SDSSTAPSVP
1630 1640 1650 1660 1670 1680
QDASGSSVPT HMDPKDQNSS VPREALAAPM QSGPGSFTVG SPAQLRGARD SGSPHKRPGQ
1690 1700 1710 1720 1730 1740
QDNSSPAKTV GRFSVVSTQD EWTLASPHSL RYSAPPDVYL DEIPSSPEVK LAVRRVQTAS
1750 1760 1770 1780 1790 1800
SIEVGVEEPA SSDSGDERPR RRSQVQKQSS LPGTGGVASD FVKKATAFLH RSSRAGSLGP
1810 1820 1830 1840 1850 1860
ETPSRAGVKV PTISITSFHS QSSYISSDND SEFEDADIKK ELRSLREKHL KEISELQSQQ
1870 1880 1890 1900 1910 1920
KQEIEALYRR LGKPLPPNVG FFHTAPPMGR RRKTSKSKLK AGKLLNPLVQ QLKVVASSTG
1930 1940 1950 1960 1970 1980
HLSDSSRGPP TKDPRGTKAV QTQQPCSVRA SLSTDICSGL ASDGGGARGQ GWTVYHPTSE
1990 2000 2010 2020 2030 2040
RGAYKSSSKP RARFLSGPVS VSIWSALKRL CLGKEHSSSL YDSPGSSTSS LAPGPEPGPQ
2050 2060 2070 2080 2090 2100
PTLHVQAQVN NSNNKKGTFT DDLHKLVDEW TTKTVGAAQV KPTLNQLKQT QKLHDMEASG
2110 2120 2130 2140
DARATSVPRA AVGASCLAPA PGPLSTTATP GATPALPVPI PDPESEKPD