Q39025

SS
Gene name
MPK5 (At4g11330, F8L21.120)
Protein name
Mitogen-activated protein kinase 5
Names
AtMPK5 , MAP kinase 5 , EC 2.7.11.24
Species
Arabidopsis thaliana (Mouse-ear cress)
KEGG Pathway
ath:AT4G11330
EC number
2.7.11.24: Protein-serine/threonine kinases
Protein Class

Descriptions

MAPK1 encodes Mitogen-activated protein kinase 1, and is involved in diverse cellular responses by phosphorylating cytoplasmic and nuclear proteins. Unlike other MAP kinases, MAPK1 activates via dual phosphorylation of Thr-186 and Tyr-193 within the activation lip, and mutations at Q105 alone were sufficient for autoactivation. Additionally, in the inactive form, L76, Q10 and I87 form a structural unit that restrains intramolecular autophosphorylation by preventing interactions between activation lip residues and the catalytic base (D149) needed for phosphoryl transfer. Mutations at L76, I87 and additionally S153 increased basal specific activity via autophosphorylation of Thr-186 and Tyr-193.

Autoinhibitory domains (AIDs)

92-171 (Autoinhibitory region) SS

Target domain

42-329 (Protein kinase domain)

Relief mechanism

PTM

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q39025

Entry ID Method Resolution Chain Position Source
AF-Q39025-F1 Predicted AlphaFoldDB

29 variants for Q39025

Variant ID(s) Position Change Description Diseaes Association Provenance
ENSVATH11500112 6 E>G No 1000Genomes
ENSVATH06616715 8 A>S No 1000Genomes
ENSVATH06616716 9 T>I No 1000Genomes
ENSVATH02786941 9 T>S No 1000Genomes
ENSVATH00490251 11 L>S No 1000Genomes
tmp_4_6892176_G_A 12 G>S No 1000Genomes
ENSVATH00490252 21 V>I No 1000Genomes
tmp_4_6892247_G_T 35 L>F No 1000Genomes
ENSVATH06616724 66 H>Y No 1000Genomes
ENSVATH14135051 75 G>A No 1000Genomes
tmp_4_6892633_T_G 79 D>E No 1000Genomes
tmp_4_6892873_G_C 136 R>P No 1000Genomes
tmp_4_6893062_A_G 174 N>D No 1000Genomes
tmp_4_6893254_A_G 238 M>V No 1000Genomes
ENSVATH06616738 254 K>R No 1000Genomes
tmp_4_6893306_T_G 255 L>R No 1000Genomes
tmp_4_6893443_G_A 269 E>K No 1000Genomes
ENSVATH06616746 274 A>E No 1000Genomes
ENSVATH14135053 276 A>T No 1000Genomes
ENSVATH06616749 318 I>M No 1000Genomes
ENSVATH00490255 326 Y>H No 1000Genomes
ENSVATH14135054 329 L>S No 1000Genomes
ENSVATH02786949 343 N>K No 1000Genomes
ENSVATH11500235 346 S>C No 1000Genomes
ENSVATH06616754 348 H>D No 1000Genomes
ENSVATH11500236 348 H>Q No 1000Genomes
tmp_4_6893838_G_A,C 375 S>N No 1000Genomes
tmp_4_6893838_G_A,C 375 S>T No 1000Genomes
tmp_4_6893843_T_A 377 I>K No 1000Genomes

No associated diseases with Q39025

4 regional properties for Q39025

Type Name Position InterPro Accession
domain Protein kinase domain 43 - 329 IPR000719
conserved_site Mitogen-activated protein (MAP) kinase, conserved site 78 - 181 IPR003527
active_site Serine/threonine-protein kinase, active site 165 - 177 IPR008271
binding_site Protein kinase, ATP binding site 49 - 73 IPR017441

Functions

Description
EC Number 2.7.11.24 Protein-serine/threonine kinases
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

2 GO annotations of cellular component

Name Definition
cytoplasm The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

3 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
MAP kinase activity Catalysis of the reaction
protein serine kinase activity Catalysis of the reactions

1 GO annotations of biological process

Name Definition
phosphorylation The process of introducing a phosphate group into a molecule, usually with the formation of a phosphoric ester, a phosphoric anhydride or a phosphoric amide.

96 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
P32485 HOG1 Mitogen-activated protein kinase HOG1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) PR
P16892 FUS3 Mitogen-activated protein kinase FUS3 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
P14681 KSS1 Mitogen-activated protein kinase KSS1 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SS
A5PKJ4 MAPK7 Mitogen-activated protein kinase 7 Bos taurus (Bovine) SS
P46196 MAPK1 Mitogen-activated protein kinase 1 Bos taurus (Bovine) SS
Q3T0N5 MAPK13 Mitogen-activated protein kinase 13 Bos taurus (Bovine) SS
Q5F3W3 MAPK6 Mitogen-activated protein kinase 6 Gallus gallus (Chicken) SS
P79996 MAPK9 Mitogen-activated protein kinase 9 Gallus gallus (Chicken) PR
Q95NE7 MAPK14 Mitogen-activated protein kinase 14 Pan troglodytes (Chimpanzee) SS
Q9N272 MAPK13 Mitogen-activated protein kinase 13 Pan troglodytes (Chimpanzee) SS
Q9W354 Erk7 Extracellular signal-regulated kinase 7 Drosophila melanogaster (Fruit fly) SS
O61443 p38b Mitogen-activated protein kinase p38b Drosophila melanogaster (Fruit fly) SS
O62618 p38a Mitogen-activated protein kinase p38a Drosophila melanogaster (Fruit fly) SS
P83100 p38c Putative mitogen-activated protein kinase 14C Drosophila melanogaster (Fruit fly) SS
P40417 rl Mitogen-activated protein kinase ERK-A Drosophila melanogaster (Fruit fly) SS
Q13164 MAPK7 Mitogen-activated protein kinase 7 Homo sapiens (Human) SS
Q8TD08 MAPK15 Mitogen-activated protein kinase 15 Homo sapiens (Human) SS
P31152 MAPK4 Mitogen-activated protein kinase 4 Homo sapiens (Human) SS
Q16659 MAPK6 Mitogen-activated protein kinase 6 Homo sapiens (Human) SS
P45983 MAPK8 Mitogen-activated protein kinase 8 Homo sapiens (Human) EV
P45984 MAPK9 Mitogen-activated protein kinase 9 Homo sapiens (Human) SS
P53779 MAPK10 Mitogen-activated protein kinase 10 Homo sapiens (Human) EV
P53778 MAPK12 Mitogen-activated protein kinase 12 Homo sapiens (Human) SS
O15264 MAPK13 Mitogen-activated protein kinase 13 Homo sapiens (Human) SS
Q15759 MAPK11 Mitogen-activated protein kinase 11 Homo sapiens (Human) SS
Q16539 MAPK14 Mitogen-activated protein kinase 14 Homo sapiens (Human) SS
P28482 MAPK1 Mitogen-activated protein kinase 1 Homo sapiens (Human) EV
P27361 MAPK3 Mitogen-activated protein kinase 3 Homo sapiens (Human) SS
Q9WVS8 Mapk7 Mitogen-activated protein kinase 7 Mus musculus (Mouse) SS
Q80Y86 Mapk15 Mitogen-activated protein kinase 15 Mus musculus (Mouse) SS
O08911 Mapk12 Mitogen-activated protein kinase 12 Mus musculus (Mouse) SS
P63085 Mapk1 Mitogen-activated protein kinase 1 Mus musculus (Mouse) SS
Q61532 Mapk6 Mitogen-activated protein kinase 6 Mus musculus (Mouse) SS
Q63844 Mapk3 Mitogen-activated protein kinase 3 Mus musculus (Mouse) SS
Q6P5G0 Mapk4 Mitogen-activated protein kinase 4 Mus musculus (Mouse) SS
Q9WUI1 Mapk11 Mitogen-activated protein kinase 11 Mus musculus (Mouse) SS
Q9Z1B7 Mapk13 Mitogen-activated protein kinase 13 Mus musculus (Mouse) SS
Q91Y86 Mapk8 Mitogen-activated protein kinase 8 Mus musculus (Mouse) PR
Q9WTU6 Mapk9 Mitogen-activated protein kinase 9 Mus musculus (Mouse) PR
P47811 Mapk14 Mitogen-activated protein kinase 14 Mus musculus (Mouse) SS
Q9Z2A6 Mapk15 Mitogen-activated protein kinase 15 Rattus norvegicus (Rat) SS
P0C865 Mapk7 Mitogen-activated protein kinase 7 Rattus norvegicus (Rat) SS
P27704 Mapk6 Mitogen-activated protein kinase 6 Rattus norvegicus (Rat) SS
P63086 Mapk1 Mitogen-activated protein kinase 1 Rattus norvegicus (Rat) SS
Q63538 Mapk12 Mitogen-activated protein kinase 12 Rattus norvegicus (Rat) SS
P49186 Mapk9 Mitogen-activated protein kinase 9 Rattus norvegicus (Rat) PR
P21708 Mapk3 Mitogen-activated protein kinase 3 Rattus norvegicus (Rat) SS
P70618 Mapk14 Mitogen-activated protein kinase 14 Rattus norvegicus (Rat) SS
Q9WTY9 Mapk13 Mitogen-activated protein kinase 13 Rattus norvegicus (Rat) SS
Q63454 Mapk4 Mitogen-activated protein kinase 4 Rattus norvegicus (Rat) SS
Q5ZCI1 MPK10 Mitogen-activated protein kinase 10 Oryza sativa subsp. japonica (Rice) SS
Q5VP69 MPK16 Mitogen-activated protein kinase 16 Oryza sativa subsp. japonica (Rice) SS
Q5SN53 MPK8 Mitogen-activated protein kinase 8 Oryza sativa subsp. japonica (Rice) SS
Q0E459 MPK13 Mitogen-activated protein kinase 13 Oryza sativa subsp. japonica (Rice) SS
Q6Z437 MPK3 Mitogen-activated protein kinase 3 Oryza sativa subsp. japonica (Rice) SS
Q10N20 MPK5 Mitogen-activated protein kinase 5 Oryza sativa subsp. japonica (Rice) SS
Q75KK8 MPK14 Mitogen-activated protein kinase 14 Oryza sativa subsp. japonica (Rice) SS
Q67C40 MPK7 Mitogen-activated protein kinase 7 Oryza sativa subsp. japonica (Rice) SS
Q6L5F7 MPK17 Mitogen-activated protein kinase 17 Oryza sativa subsp. japonica (Rice) SS
Q84UI5 MPK1 Mitogen-activated protein kinase 1 Oryza sativa subsp. japonica (Rice) SS
Q5VN19 MPK11 Mitogen-activated protein kinase 11 Oryza sativa subsp. japonica (Rice) SS
Q5Z859 MPK4 Mitogen-activated protein kinase 4 Oryza sativa subsp. japonica (Rice) SS
Q5Z9J0 MPK12 Mitogen-activated protein kinase 12 Oryza sativa subsp. japonica (Rice) SS
Q53N72 MPK15 Mitogen-activated protein kinase 15 Oryza sativa subsp. japonica (Rice) SS
Q336X9 MPK6 Mitogen-activated protein kinase 6 Oryza sativa subsp. japonica (Rice) SS
Q5J4W4 MPK2 Mitogen-activated protein kinase 2 Oryza sativa subsp. japonica (Rice) SS
Q11179 mapk-15 Mitogen-activated protein kinase 15 Caenorhabditis elegans SS
O44514 pmk-3 Mitogen-activated protein kinase pmk-3 Caenorhabditis elegans PR
G5EBT1 sma-5 Mitogen-activated protein kinase sma-5 Caenorhabditis elegans SS
Q17446 pmk-1 Mitogen-activated protein kinase pmk-1 Caenorhabditis elegans SS
Q8MXI4 pmk-2 Mitogen-activated protein kinase pmk-2 Caenorhabditis elegans SS
O44408 kgb-1 GLH-binding kinase 1 Caenorhabditis elegans PR
P39745 mpk-1 Mitogen-activated protein kinase mpk-1 Caenorhabditis elegans SS
O23236 MPK14 Mitogen-activated protein kinase 14 Arabidopsis thaliana (Mouse-ear cress) SS
Q39021 MPK1 Mitogen-activated protein kinase 1 Arabidopsis thaliana (Mouse-ear cress) SS
Q39022 MPK2 Mitogen-activated protein kinase 2 Arabidopsis thaliana (Mouse-ear cress) SS
Q39023 MPK3 Mitogen-activated protein kinase 3 Arabidopsis thaliana (Mouse-ear cress) SS
Q39024 MPK4 Mitogen-activated protein kinase 4 Arabidopsis thaliana (Mouse-ear cress) SS
Q39026 MPK6 Mitogen-activated protein kinase 6 Arabidopsis thaliana (Mouse-ear cress) SS
Q39027 MPK7 Mitogen-activated protein kinase 7 Arabidopsis thaliana (Mouse-ear cress) SS
Q84M93 MPK17 Mitogen-activated protein kinase 17 Arabidopsis thaliana (Mouse-ear cress) SS
Q8W4J2 MPK16 Mitogen-activated protein kinase 16 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C5C0 MPK18 Mitogen-activated protein kinase 18 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LM33 MPK8 Mitogen-activated protein kinase 8 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LUC3 MPK19 Mitogen-activated protein kinase 19 Arabidopsis thaliana (Mouse-ear cress) SS
Q9M1Z5 MPK10 Mitogen-activated protein kinase 10 Arabidopsis thaliana (Mouse-ear cress) SS
Q9SJG9 MPK20 Mitogen-activated protein kinase 20 Arabidopsis thaliana (Mouse-ear cress) SS
Q8GYQ5 MPK12 Mitogen-activated protein kinase 12 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LV37 MPK9 Mitogen-activated protein kinase 9 Arabidopsis thaliana (Mouse-ear cress) SS
Q9C9U4 MPK15 Mitogen-activated protein kinase 15 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LMM5 MPK11 Mitogen-activated protein kinase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q9LQQ9 MPK13 Mitogen-activated protein kinase 13 Arabidopsis thaliana (Mouse-ear cress) SS
P43294 MHK Serine/threonine-protein kinase MHK Arabidopsis thaliana (Mouse-ear cress) PR
O42376 mapk12 Mitogen-activated protein kinase 12 Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9DGE2 mapk14a Mitogen-activated protein kinase 14A Danio rerio (Zebrafish) (Brachydanio rerio) SS
Q9DGE1 mapk14b Mitogen-activated protein kinase 14B Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MAKEIESATD LGDTNIKGVL VHGGRYFQYN VYGNLFEVSN KYVPPIRPIG RGAYGFVCAA
70 80 90 100 110 120
VDSETHEEIA IKKIGKAFDN KVDAKRTLRE IKLLRHLEHE NVVVIKDIIR PPKKEDFVDV
130 140 150 160 170 180
YIVFELMDTD LHQIIRSNQS LNDDHCQYFL YQILRGLKYI HSANVLHRDL KPSNLLLNSN
190 200 210 220 230 240
CDLKITDFGL ARTTSETEYM TEYVVTRWYR APELLLNSSE YTSAIDVWSV GCIFAEIMTR
250 260 270 280 290 300
EPLFPGKDYV HQLKLITELI GSPDGASLEF LRSANARKYV KELPKFPRQN FSARFPSMNS
310 320 330 340 350 360
TAIDLLEKML VFDPVKRITV EEALCYPYLS ALHDLNDEPV CSNHFSFHFE DPSSTEEEIK
370
ELVWLESVKF NPLPSI