Q2R1M8

SS
Gene name
Os11g0599500 (LOC_Os11g38670)
Protein name
DEAD-box ATP-dependent RNA helicase 52C
Names
EC 3.6.4.13
Species
Oryza sativa subsp. japonica (Rice)
KEGG Pathway
osa:4350856
EC number
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement
Protein Class

Descriptions

DEAD-box proteins utilize ATP to bind and remodel RNA and RNA-protein complexes. The Ded1/DDX3 subfamily of DEAD-box proteins is of particular interest as their function during protein translation, are essential for viability, and are frequently altered in human malignancies. <br>There is a unique interdomain interaction between the two ATPase domains (DEAD box helicase domain and helicase domain) in which the C-terminal helicase domain clashes with the RNA-binding surface. Destabilizing this interaction accelerates RNA duplex unwinding, suggesting that it is present in solution and inhibitory for catalysis. The N-terminal extension to the DEAD box helicase interacts with DEAD box helicase domain and C-terminal helicase domain, and stabilizes the autoinhibited state of DDX3X.

Autoinhibitory domains (AIDs)

383-550 (C-terminal helicase domain) SS

Target domain

179-360 (DEAD box helicase domain)

Relief mechanism

Assay

179-360 (DEAD box helicase domain) SS

Target domain

383-550 (C-terminal helicase domain)

Relief mechanism

Assay

106-157 (N-terminal extension) SS

Target domain

179-360 (DEAD box helicase domain);383-550 (C-terminal helicase domain)

Relief mechanism

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q2R1M8

Entry ID Method Resolution Chain Position Source
AF-Q2R1M8-F1 Predicted AlphaFoldDB

No variants for Q2R1M8

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q2R1M8

No associated diseases with Q2R1M8

7 regional properties for Q2R1M8

Type Name Position InterPro Accession
domain Dbl homology (DH) domain 49 - 240 IPR000219
domain DEP domain 421 - 496 IPR000591-1
domain DEP domain 524 - 597 IPR000591-2
conserved_site Guanine-nucleotide dissociation stimulator, CDC24, conserved site 188 - 213 IPR001331
domain PDZ domain 625 - 706 IPR001478-1
domain PDZ domain 717 - 788 IPR001478-2
domain Pleckstrin homology domain 271 - 394 IPR001849

Functions

Description
EC Number 3.6.4.13 Acting on ATP; involved in cellular and subcellular movement
Subcellular Localization
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

1 GO annotations of cellular component

Name Definition
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
ATP binding Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.
ATP hydrolysis activity Catalysis of the reaction
mRNA binding Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.
RNA helicase activity Unwinding of an RNA helix, driven by ATP hydrolysis.

No GO annotations of biological process

Name Definition
No GO annotations for biological process

27 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q5W5U4 DDX4 Probable ATP-dependent RNA helicase DDX4 Bos taurus (Bovine) SS
Q6GVM6 DDX3Y ATP-dependent RNA helicase DDX3Y Pan troglodytes (Chimpanzee) SS
O15523 DDX3Y ATP-dependent RNA helicase DDX3Y Homo sapiens (Human) SS
Q9NQI0 DDX4 Probable ATP-dependent RNA helicase DDX4 Homo sapiens (Human) SS
O00571 DDX3X ATP-dependent RNA helicase DDX3X Homo sapiens (Human) EV
P16381 D1Pas1 Putative ATP-dependent RNA helicase Pl10 Mus musculus (Mouse) SS
Q61496 Ddx4 ATP-dependent RNA helicase DDX4 Mus musculus (Mouse) SS
Q62095 Ddx3y ATP-dependent RNA helicase DDX3Y Mus musculus (Mouse) SS
Q62167 Ddx3x ATP-dependent RNA helicase DDX3X Mus musculus (Mouse) SS
Q6GWX0 DDX4 Probable ATP-dependent RNA helicase DDX4 Sus scrofa (Pig) SS
Q6Z4K6 PL10B DEAD-box ATP-dependent RNA helicase 52B Oryza sativa subsp. japonica (Rice) SS
Q75HJ0 PL10A DEAD-box ATP-dependent RNA helicase 37 Oryza sativa subsp. japonica (Rice) SS
Q5QMN3 Os01g0197200 DEAD-box ATP-dependent RNA helicase 20 Oryza sativa subsp japonica (Rice) PR
Q5JK84 AIP2 DEAD-box ATP-dependent RNA helicase 15 Oryza sativa subsp japonica (Rice) PR
Q5VNM3 EIF4A3A Eukaryotic initiation factor 4A-III homolog A Oryza sativa subsp japonica (Rice) PR
Q0E2Z7 Os02g0201900 DEAD-box ATP-dependent RNA helicase 41 Oryza sativa subsp japonica (Rice) PR
Q10I26 EIF4A3B Eukaryotic initiation factor 4A-III homolog B Oryza sativa subsp japonica (Rice) PR
Q10RI7 Os03g0158200 DEAD-box ATP-dependent RNA helicase 38 Oryza sativa subsp japonica (Rice) PR
Q5Z6G5 Os06g0697200 DEAD-box ATP-dependent RNA helicase 35B Oryza sativa subsp japonica (Rice) PR
Q0JM17 AIP1 DEAD-box ATP-dependent RNA helicase 56 Oryza sativa subsp japonica (Rice) PR
Q5N7W4 Os01g0911100 DEAD-box ATP-dependent RNA helicase 30 Oryza sativa subsp japonica (Rice) PR
Q0E3X4 Os02g0150100 DEAD-box ATP-dependent RNA helicase 35A Oryza sativa subsp japonica (Rice) PR
O01836 glh-3 ATP-dependent RNA helicase glh-3 Caenorhabditis elegans PR
D0PV95 laf-1 ATP-dependent RNA helicase laf-1 Caenorhabditis elegans SS
Q84W89 RH37 DEAD-box ATP-dependent RNA helicase 37 Arabidopsis thaliana (Mouse-ear cress) SS
Q8LA13 RH11 DEAD-box ATP-dependent RNA helicase 11 Arabidopsis thaliana (Mouse-ear cress) SS
Q9M2F9 RH52 DEAD-box ATP-dependent RNA helicase 52 Arabidopsis thaliana (Mouse-ear cress) SS
10 20 30 40 50 60
MATPSRTSWA DVADADPAPA PAPAANGPAR PDRSSYVPPH LRNRGASSGG GAAAPPPSSS
70 80 90 100 110 120
SSSAPPPRAA PGLLAPRPAA AGMGRMGGGG GGGGFGGPRR WDREPNPFGN DGDAAAGAGD
130 140 150 160 170 180
EPEVFDAHQN TGINFDAYED IPVETSGREV PPPVGTFAEI DLGQALNDNI RRCKYVRPTP
190 200 210 220 230 240
VQRYAIPISL AGRDLMACAQ TGSGKTAAFC FPIISGIMRG PPAQRPQRGG MRTACPLALI
250 260 270 280 290 300
LSPTRELSMQ IHEEARKFSY QTGVRVVVAY GGAPINQQLR DLERGVDILV ATPGRLVDLL
310 320 330 340 350 360
ERARVSLQSI RYLALDEADR MLDMGFEPQV RRIVEQMDMP PPGARQTMLF SATFPKEIQR
370 380 390 400 410 420
MASDFLENYI FLAVGRVGSS TDLIVQRVEF VQEADKRSHL MDLLHAQRDS ATPGKPTLTL
430 440 450 460 470 480
VFVETKRGAD SLEHWLCMNG FPATSIHGDR NQQEREYALR SFKSGHTPIL VATDVAARGL
490 500 510 520 530 540
DIPHVAHVVN FDLPNDIDDY VHRIGRTGRA GKSGLATAFF NENNSSMARS LAELMQESNQ
550 560 570 580 590 600
EVPAWLSRYA ARPSYGGGGG RNRRSGGGSR FGGRDFRRDS SSGRGGGDYY GGGSSGGAGG
610 620
YGGSSAYGGG GYGGGAGAPS AWD