Q2NL22
PR
Gene name |
EIF4A3 (DDX48) |
Protein name |
Eukaryotic initiation factor 4A-III |
Names |
ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic translation initiation factor 4A isoform 3 |
Species |
Bos taurus (Bovine) |
KEGG Pathway |
bta:515145 |
EC number |
3.6.4.13: Acting on ATP; involved in cellular and subcellular movement |
Protein Class |
|
Descriptions
The autoinhibited protein was predicted that may have potential autoinhibitory elements via cis-regPred.
Autoinhibitory domains (AIDs)
Target domain |
|
Relief mechanism |
|
Assay |
cis-regPred |
Accessory elements
No accessory elements
Autoinhibited structure
Activated structure
1 structures for Q2NL22
| Entry ID | Method | Resolution | Chain | Position | Source |
|---|---|---|---|---|---|
| AF-Q2NL22-F1 | Predicted | AlphaFoldDB |
26 variants for Q2NL22
| Variant ID(s) | Position | Change | Description | Diseaes Association | Provenance |
|---|---|---|---|---|---|
| rs448711494 | 3 | A>G | No | EVA | |
| rs466511037 | 21 | E>K | No | EVA | |
| rs432135549 | 23 | M>I | No | EVA | |
| rs452274815 | 24 | T>P | No | EVA | |
| rs452274815 | 24 | T>S | No | EVA | |
| rs475653098 | 35 | D>E | No | EVA | |
| rs437868135 | 38 | P>R | No | EVA | |
| rs454854406 | 48 | D>A | No | EVA | |
| rs463503704 | 57 | G>V | No | EVA | |
| rs480371578 | 64 | I>T | No | EVA | |
| rs449204193 | 66 | Q>* | No | EVA | |
| rs466042172 | 78 | V>L | No | EVA | |
| rs479961198 | 81 | Q>L | No | EVA | |
| rs439290099 | 139 | A>T | No | EVA | |
| rs482767508 | 147 | G>R | No | EVA | |
| rs441850212 | 150 | I>F | No | EVA | |
| rs461996805 | 151 | R>M | No | EVA | |
| rs478926997 | 158 | H>D | No | EVA | |
| rs451462732 | 205 | Y>N | No | EVA | |
| rs471441328 | 217 | I>L | No | EVA | |
| rs132659221 | 258 | V>G | No | EVA | |
| rs433473259 | 294 | T>A | No | EVA | |
| rs464845231 | 295 | E>G | No | EVA | |
| rs481703705 | 297 | M>K | No | EVA | |
| rs450533747 | 299 | E>G | No | EVA | |
| rs462060146 | 329 | R>L | No | EVA |
No associated diseases with Q2NL22
3 regional properties for Q2NL22
Functions
| Description | ||
|---|---|---|
| EC Number | 3.6.4.13 | Acting on ATP; involved in cellular and subcellular movement |
| Subcellular Localization |
|
|
| PANTHER Family | ||
| PANTHER Subfamily | ||
| PANTHER Protein Class | ||
| PANTHER Pathway Category | No pathway information available | |
7 GO annotations of cellular component
| Name | Definition |
|---|---|
| catalytic step 2 spliceosome | A spliceosomal complex that contains three snRNPs, including U5, bound to a splicing intermediate in which the first catalytic cleavage of the 5' splice site has occurred. The precise subunit composition differs significantly from that of the catalytic step 1, or activated, spliceosome, and includes many proteins in addition to those found in the associated snRNPs. |
| cytoplasm | The contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures. |
| exon-exon junction complex | A multi-subunit complex deposited by the spliceosome upstream of messenger RNA exon-exon junctions. The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay. |
| nuclear speck | A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy. |
| nucleolus | A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome. |
| nucleus | A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent. |
| U2-type catalytic step 1 spliceosome | A spliceosomal complex that is formed by the displacement of the U1 and U4 snRNPs from the precatalytic spliceosome; the U2, U5 and U6 snRNPs remain associated with the mRNA. This complex, sometimes called the activated spliceosome, is the catalytically active form of the spliceosome, and includes many proteins in addition to those found in the U2, and U5 and U6 snRNPs. |
6 GO annotations of molecular function
| Name | Definition |
|---|---|
| ATP binding | Binding to ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator. |
| ATP hydrolysis activity | Catalysis of the reaction: ATP + H2O = ADP + H+ phosphate. ATP hydrolysis is used in some reactions as an energy source, for example to catalyze a reaction or drive transport against a concentration gradient. |
| mRNA binding | Binding to messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns. |
| poly(A) binding | Binding to a sequence of adenylyl residues in an RNA molecule, such as the poly(A) tail, a sequence of adenylyl residues at the 3' end of eukaryotic mRNA. |
| RNA binding | Binding to an RNA molecule or a portion thereof. |
| RNA helicase activity | Unwinding of an RNA helix, driven by ATP hydrolysis. |
9 GO annotations of biological process
| Name | Definition |
|---|---|
| embryonic cranial skeleton morphogenesis | The process in which the anatomical structures of the cranial skeleton are generated and organized during the embryonic phase. |
| mRNA splicing, via spliceosome | The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced. |
| mRNA transport | The directed movement of mRNA, messenger ribonucleic acid, into, out of or within a cell, or between cells, by means of some agent such as a transporter or pore. |
| negative regulation of translation | Any process that stops, prevents, or reduces the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA. |
| nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | The nonsense-mediated decay pathway for nuclear-transcribed mRNAs degrades mRNAs in which an amino-acid codon has changed to a nonsense codon; this prevents the translation of such mRNAs into truncated, and potentially harmful, proteins. |
| positive regulation of translation | Any process that activates or increases the frequency, rate or extent of the chemical reactions and pathways resulting in the formation of proteins by the translation of mRNA or circRNA. |
| regulation of alternative mRNA splicing, via spliceosome | Any process that modulates the frequency, rate or extent of alternative splicing of nuclear mRNAs. |
| regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay | Any process that modulates the frequency, rate or extent of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay. |
| rRNA processing | Any process involved in the conversion of a primary ribosomal RNA (rRNA) transcript into one or more mature rRNA molecules. |
18 homologous proteins in AiPD
| UniProt AC | Gene Name | Protein Name | Species | Evidence Code |
|---|---|---|---|---|
| Q12099 | FAL1 | ATP-dependent RNA helicase FAL1 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | PR |
| Q0IIK5 | DDX1 | ATP-dependent RNA helicase DDX1 | Bos taurus (Bovine) | PR |
| Q3SZ65 | EIF4A2 | Eukaryotic initiation factor 4A-II | Bos taurus (Bovine) | PR |
| Q3T147 | DDX39B | Spliceosome RNA helicase DDX39B | Bos taurus (Bovine) | PR |
| Q5W5U4 | DDX4 | Probable ATP-dependent RNA helicase DDX4 | Bos taurus (Bovine) | SS |
| Q2TBP1 | DDX25 | ATP-dependent RNA helicase DDX25 | Bos taurus (Bovine) | PR |
| Q3ZBV2 | DDX19A | ATP-dependent RNA helicase DDX19A | Bos taurus (Bovine) | SS |
| Q5ZM36 | EIF4A3 | Eukaryotic initiation factor 4A-III | Gallus gallus (Chicken) | PR |
| Q9VHS8 | CG7483 | Eukaryotic initiation factor 4A-III | Drosophila melanogaster (Fruit fly) | PR |
| P38919 | EIF4A3 | Eukaryotic initiation factor 4A-III | Homo sapiens (Human) | PR |
| Q91VC3 | Eif4a3 | Eukaryotic initiation factor 4A-III | Mus musculus (Mouse) | PR |
| A6M931 | EIF4A3 | Eukaryotic initiation factor 4A-III | Sus scrofa (Pig) | PR |
| Q3B8Q2 | Eif4a3 | Eukaryotic initiation factor 4A-III | Rattus norvegicus (Rat) | PR |
| Q10I26 | EIF4A3B | Eukaryotic initiation factor 4A-III homolog B | Oryza sativa subsp japonica (Rice) | PR |
| Q5VNM3 | EIF4A3A | Eukaryotic initiation factor 4A-III homolog A | Oryza sativa subsp japonica (Rice) | PR |
| Q94A52 | EIF4A3 | Eukaryotic initiation factor 4A-III homolog | Arabidopsis thaliana (Mouse-ear cress) | PR |
| B7ZTW1 | eif4a3 | Eukaryotic initiation factor 4A-III | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) | PR |
| Q7ZVA6 | eif4a3 | Eukaryotic initiation factor 4A-III | Danio rerio (Zebrafish) (Brachydanio rerio) | PR |
| 10 | 20 | 30 | 40 | 50 | 60 |
| MAATATMATS | GSARKRLLKE | EDMTKVEFET | SEEVDVTPTF | DTMGLREDLL | RGIYAYGFEK |
| 70 | 80 | 90 | 100 | 110 | 120 |
| PSAIQQRAIK | QIIKGRDVIA | QSQSGTGKTA | TFSISVLQCL | DIQVRETQAL | ILAPTRELAV |
| 130 | 140 | 150 | 160 | 170 | 180 |
| QIQKGLLALG | DYMNVQCHAC | IGGTNVGEDI | RKLDYGQHVV | AGTPGRVFDM | IRRRSLRTRA |
| 190 | 200 | 210 | 220 | 230 | 240 |
| IKMLVLDEAD | EMLNKGFKEQ | IYDVYRYLPP | ATQVVLISAT | LPHEILEMTN | KFMTDPIRIL |
| 250 | 260 | 270 | 280 | 290 | 300 |
| VKRDELTLEG | IKQFFVAVER | EEWKFDTLCD | LYDTLTITQA | VIFCNTKRKV | DWLTEKMREA |
| 310 | 320 | 330 | 340 | 350 | 360 |
| NFTVSSMHGD | MPQKERESIM | KEFRSGASRV | LISTDVWARG | LDVPQVSLII | NYDLPNNREL |
| 370 | 380 | 390 | 400 | 410 | |
| YIHRIGRSGR | YGRKGVAINF | VKNDDIRILR | DIEQYYSTQI | DEMPMNVADL | I |