Q28DL4

SS
Gene name
traf6 (TNeu045k15.1)
Protein name
TNF receptor-associated factor 6
Names
EC 2.3.2.27 , E3 ubiquitin-protein ligase TRAF6 , RING-type E3 ubiquitin transferase TRAF6
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
KEGG Pathway
xtr:493524
EC number
2.3.2.27: Aminoacyltransferases
Protein Class

Descriptions

The Tumor Necrosis Factor (TNF) Receptor Associated Factor (TRAF) class of intracellular signal transducers is responsible for mediating many of the activation events initiated by TNF receptor (TNFR) and Toll-like/Interleukin-1, 17 and 18 receptor (TIR) families. ​​These receptors can, both directly and indirectly, activate TRAF6 via specific binding of proteins containing TRAF Interacting Motif (TIM) peptides to a groove on the carboxyl-terminal MATH domain. <br>TRAF6 is autoinhibited by an intramolecular interaction between the RING-Zinc (RZ) region and MATH domain. The interaction renders TRAF6 inactive and structurally closed, as well as incapable of auto-ubiquitination. RING-Zinc fingers region and the MATH domain are targets for ubiquitination. TRAF autoubiquitination in trans is a means of sustaining an open conformation active in downstream signaling.

Autoinhibitory domains (AIDs)

382-558 (MATH domain) SS

Target domain

72-275 (RZ domain)

Relief mechanism

Partner binding

Assay

Accessory elements

No accessory elements

References

Autoinhibited structure

Activated structure

1 structures for Q28DL4

Entry ID Method Resolution Chain Position Source
AF-Q28DL4-F1 Predicted AlphaFoldDB

No variants for Q28DL4

Variant ID(s) Position Change Description Diseaes Association Provenance
No variants for Q28DL4

No associated diseases with Q28DL4

13 regional properties for Q28DL4

Type Name Position InterPro Accession
domain C2 domain 157 - 276 IPR000008
domain Protein kinase domain 339 - 597 IPR000719
domain AGC-kinase, C-terminal 598 - 668 IPR000961
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 35 - 86 IPR002219-1
domain Protein kinase C-like, phorbol ester/diacylglycerol-binding domain 100 - 152 IPR002219-2
active_site Serine/threonine-protein kinase, active site 459 - 471 IPR008271
binding_site Protein kinase, ATP binding site 345 - 368 IPR017441
domain Protein kinase, C-terminal 622 - 658 IPR017892
domain Diacylglycerol/phorbol-ester binding 33 - 47 IPR020454-1
domain Diacylglycerol/phorbol-ester binding 49 - 58 IPR020454-2
domain Diacylglycerol/phorbol-ester binding 62 - 73 IPR020454-3
domain Diacylglycerol/phorbol-ester binding 139 - 151 IPR020454-4
domain Classical protein kinase C beta, catalytic domain 338 - 660 IPR034664

Functions

Description
EC Number 2.3.2.27 Aminoacyltransferases
Subcellular Localization
  • Cytoplasm
  • Cytoplasm, cell cortex
  • Nucleus
  • Lipid droplet
PANTHER Family
PANTHER Subfamily
PANTHER Protein Class
PANTHER Pathway Category No pathway information available

3 GO annotations of cellular component

Name Definition
cell cortex The region of a cell that lies just beneath the plasma membrane and often, but not always, contains a network of actin filaments and associated proteins.
lipid droplet An intracellular non-membrane-bounded organelle comprising a matrix of coalesced lipids surrounded by a phospholipid monolayer. May include associated proteins.
nucleus A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

4 GO annotations of molecular function

Name Definition
tumor necrosis factor receptor binding Binding to a tumor necrosis factor receptor.
ubiquitin protein ligase activity Catalysis of the transfer of ubiquitin to a substrate protein via the reaction X-ubiquitin + S -> X + S-ubiquitin, where X is either an E2 or E3 enzyme, the X-ubiquitin linkage is a thioester bond, and the S-ubiquitin linkage is an amide bond
ubiquitin-protein transferase activity Catalysis of the transfer of ubiquitin from one protein to another via the reaction X-Ub + Y --> Y-Ub + X, where both X-Ub and Y-Ub are covalent linkages.
zinc ion binding Binding to a zinc ion (Zn).

7 GO annotations of biological process

Name Definition
innate immune response Innate immune responses are defense responses mediated by germline encoded components that directly recognize components of potential pathogens.
intracellular signal transduction The process in which a signal is passed on to downstream components within the cell, which become activated themselves to further propagate the signal and finally trigger a change in the function or state of the cell.
lipopolysaccharide-mediated signaling pathway The series of molecular signals initiated by the binding of a lipopolysaccharide (LPS) to a receptor on the surface of a target cell, and ending with the regulation of a downstream cellular process, e.g. transcription. Lipopolysaccharides are major components of the outer membrane of Gram-negative bacteria, making them prime targets for recognition by the immune system.
protein K63-linked ubiquitination A protein ubiquitination process in which a polymer of ubiquitin, formed by linkages between lysine residues at position 63 of the ubiquitin monomers, is added to a protein. K63-linked ubiquitination does not target the substrate protein for degradation, but is involved in several pathways, notably as a signal to promote error-free DNA postreplication repair.
regulation of apoptotic process Any process that modulates the occurrence or rate of cell death by apoptotic process.
regulation of canonical NF-kappaB signal transduction Any process that modulates I-kappaB kinase/NF-kappaB signaling.
tumor necrosis factor-mediated signaling pathway The series of molecular signals initiated by tumor necrosis factor binding to its receptor on the surface of a cell, and ending with the regulation of a downstream cellular process, e.g. transcription.

7 homologous proteins in AiPD

UniProt AC Gene Name Protein Name Species Evidence Code
Q3ZCC3 TRAF6 TNF receptor-associated factor 6 Bos taurus (Bovine) SS
Q9Y4K3 TRAF6 TNF receptor-associated factor 6 Homo sapiens (Human) EV
P70196 Traf6 TNF receptor-associated factor 6 Mus musculus (Mouse) SS
A7XUJ6 TRAF6 TNF receptor-associated factor 6 Sus scrofa (Pig) SS
B5DF45 Traf6 TNF receptor-associated factor 6 Rattus norvegicus (Rat) SS
B6CJY5 TRAF6 TNF receptor-associated factor 6 Macaca mulatta (Rhesus macaque) SS
Q6IWL4 traf6 TNF receptor-associated factor 6 Danio rerio (Zebrafish) (Brachydanio rerio) SS
10 20 30 40 50 60
MSILNCRPSF DGVDTDDACC GAMASACCVN TKEDGESPSA GSPSGTPQSL VLEDVQGYDV
70 80 90 100 110 120
EFDPPLESKY ECPICLMALR EAVQTPCGHR FCKACILKSI RDAGHKCPVD NESLMENQLF
130 140 150 160 170 180
PDNFAKREIL SLRVKCPSQG CTETMELRHL ERHLVRCDFA GVECSQCQSS FPKYSLQKHK
190 200 210 220 230 240
FEECPRRQIF CENCAVAMAL EDKLNHDQTC PLAYVTCEYC QTNLIREQMP AHYSMDCTMA
250 260 270 280 290 300
PIPCMYYEFG CTEKMQRNDL ARHLQEFTQA HMRMMAQTLR SFSSSVTPTS HMPDISFCDP
310 320 330 340 350 360
SQFEPAPPSV ATVHSTHTPS QNDCTQETRN LRETIEQLEG RLVRQDHQIR ELIAKMETQC
370 380 390 400 410 420
TYVNELKHTI RSLDNRLGEM ESQQCSGIFI WRINNFNSLL KNQEEERPVV IHSQGFYTGK
430 440 450 460 470 480
PGYKLCLRLH LQLPSAQRCA NYISLFVHTM QGEYDSLLPW PLQGTIRLSI LDQSEGVAMQ
490 500 510 520 530 540
DQEEVMDTKP ELLAFQRPTV ARNPKGFGYV TFMHLQALKQ RQYVKNDTLL VRCSVTTHLD
550
LISPRREGFQ PRSGDGAL